LOCUS       sequence-41114-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00444
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      604..613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             670..687
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             676..693
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 atggatacga caccaatctt ccagtccagc ttctccatcc gatcgctgct ctccgtggac
       61 aaaaaggagg agtcccccat ttcgaagcac aactcaggaa gcagcttcag ctcatgcccg
      121 agctccagtt ccaactccag ctcggattcg atggctgcaa agagcaatgc aaagccggct
      181 ttcacctaca gcgccctcat agtgatggcc atctggagca gctccgagaa gcgtttgacc
      241 ctaagcggga tctgcaagtg gatcgcggac aacttcccgt actatcgcac ccgcaagagc
      301 gtctggcaga actcgatccg gcacaacctg agtctcaatc cgttcttcgt tcgggtgccg
      361 cgagctctcg atgatcctgg acgtggccac tactgggcac tcgatccgta tgccgaggat
      421 ctgtccattg gcgagacgac gggacgcctg cgccgcagca attggcagca gaatacggtg
      481 gcgagaccca aggtcacagg tcatccctat cagcgaatgc catattatgg gcacgggcac
      541 ggtaacggcc catatatcaa ggcacacagc gcctacttcc ccataatgga ccatcaacac
      601 catgccgcca tggtgcagca ctaccaggcc atgatgcaca gataccagat gatgccacat
      661 cctcaccatc accagcatca gcatcagcat cagcatcctc attctcattt cattcagcaa
      721 tcaaagcccc ttcatatcca ggagccatat catcataccc gctaccatct tcaccaagag
//
