LOCUS       sequence-411143-        9380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for biosynthetic pathway. pR-phiC31 integrase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
  TITLE     Cross-kingdom expression of synthetic genetic elements promotes 
            discovery of metabolites in the human microbiome.
  JOURNAL   Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 
            10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
  PUBMED    35366417
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.008"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1487-1505.e14"
COMMENT     Sequence Label: pPath-pR-integrase
FEATURES             Location/Qualifiers
     source          1..9380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(34..52)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(35..52)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    55..88
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        344..687
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             718..1527
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDVSD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(785..804)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1533..1730
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             1867..1884
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1937..1955)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1951..1998
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     2017..2036
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2017..2035
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2074..2519
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(2520..2606,2607..3941)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        4141..4232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4233..4301,4302..5093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      5097..5136
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            5283..5391
                     /note="incP origin of transfer"
     rep_origin      5410..5798
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             join(7596..9380,1..33)
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 gcccaggacg gcacggaaga cgtagcggcg taatctatag tgtcacctaa atgggtgcca
       61 gggcgtgccc gtgggctccc cgggcgcgta tagggataac agggtaatac gtcaaattct
      121 atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
      181 acaactttga aaaggctgtt ttctgtattt aaggttttag aatgcaagga acagtgaatt
      241 ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaaagaaa
      301 ttgcatacct ttgttcctcg gttatatgtt tgctcatctg caagacatgg aggcccagaa
      361 taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta
      421 catttagccc atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac
      481 ggcgcgaagc aaaaattacg gcacctcgct gcagacctgc gagcagggaa acgctcccct
      541 cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga
      601 tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc
      661 tcacatcaca tccgaacata aacaaccaaa aatttgtaat taagaaggag tgattacatg
      721 ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt ccaacatgga tgctgattta
      781 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg
      841 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
      901 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
      961 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggc
     1021 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1081 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     1141 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgtg
     1201 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     1261 aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     1321 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     1381 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     1441 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     1501 catttgatgc tcgatgagtt tttctaatca gtactgacaa taaaaagatt cttgttttca
     1561 agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta
     1621 gcgtgattta tatttttttt cgcctcgaca tcatctgtcc agatgcgaag ttaagtgcgc
     1681 agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctctagcata
     1741 accccgcggg gcctctttcg gggatctcgc ggggtttttt gctgaaagaa gcttcaaata
     1801 aaacgaaagg ctcagtcgaa agactgggcc tttcgttatg ttgttgtcgc tgcggccgca
     1861 ctcgagcacc accaccacca ccactgggat ccggctgcta acaaagcccg aaaggaagct
     1921 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     1981 gtcttgaggg gttttttgct gaaggccatc atggcctaat acgactcact atagggagat
     2041 cctgcaggcc aatgtgatgg acactgagac ctccacatat acctgccgtt cactattatt
     2101 tagtgaaatg agatattatg atattttctg aattgtgatt aaaaaggcaa ctttatgccc
     2161 atgcaacaga aactataaaa aatacagaga atgaaaagaa acagatagat tttttagttc
     2221 tttaggcccg tagtctgcaa atccttttat gattttctat caaacaaaag aggaaaatag
     2281 accagttgca atccaaacga gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt
     2341 ttgttactga taaagcaggc aagacctaaa atgtgtaaag ggcaaagtgt atactttggc
     2401 gtcacccctt acatatttta ggtctttttt tattgtgcgt aactaacttg ccatcttcaa
     2461 acaggagggc tggaagaagc agaccgctaa cacagtacat aaaaaaggag acatgaacga
     2521 tgaacatcaa aaagtttgca aaacaagcaa cagtattaac ctttactacc gcactgctgg
     2581 caggaggcgc aactcaagcg tttgcgaaag aaacgaacca aaagccatat aaggaaacat
     2641 acggcatttc acatattaca cgccatgata tgctgcaaat ccctgaacag caaaaaaatg
     2701 aaaaatatca agttcctgag ttcgattcgt ccacaattaa aaatatctct tctgcaaaag
     2761 gcctggacgt ttgggacagc tggccattac aaaacgctga cggcactgtc gcaaactatc
     2821 acggctacca catcgtcttt gcattagccg gagatcctaa aaatgcggat gacacatcga
     2881 tttacatgtt ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa aacgctggcc
     2941 gcgtctttaa agacagcgac aaattcgatg caaatgattc tatcctaaaa gaccaaacac
     3001 aagaatggtc aggttcagcc acatttacat ctgacggaaa aatccgttta ttctacactg
     3061 atttctccgg taaacattac ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag
     3121 catcagacag ctctttgaac atcaacggtg tagaggatta taaatcaatc tttgacggtg
     3181 acggaaaaac gtatcaaaat gtacagcagt tcatcgatga aggcaactac agctcaggcg
     3241 acaaccatac gctgagagat cctcactacg tagaagataa aggccacaaa tacttagtat
     3301 ttgaagcaaa cactggaact gaagatggct accaaggcga agaatcttta tttaacaaag
     3361 catactatgg caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg
     3421 ataaaaaacg cacggctgag ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg
     3481 attacacact gaaaaaagtg atgaaaccgc tgattgcatc taacacagta acagatgaaa
     3541 ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta cctgttcact gactcccgcg
     3601 gatcaaaaat gacgattgac ggcattacgt ctaacgatat ttacatgctt ggttatgttt
     3661 ctaattcttt aactggccca tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg
     3721 atcttgatcc taacgatgta acctttactt actcacactt cgctgtacct caagcgaaag
     3781 gaaacaatgt cgtgattaca agctatatga caaacagagg attctacgca gacaaacaat
     3841 caacgtttgc gccaagtttc ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag
     3901 acagcatcct tgaacaagga caattaacag ttaacaaata aggtctctag agccaccaca
     3961 gtggttaatt aaggcctgtg aggccggacc aaaacgaaaa aaggcccccc tttcgggagg
     4021 cctcttttct ggaatttggt accgaggggt gccagggcgt gccccagggc tccccgggcg
     4081 cgtataatcg atttaaatta gtagcccgcc taatgagcgg gctttttttt aattccccta
     4141 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4201 aaatgcttca ataatattga aaaaggaaga gtatgagcat tcagcatttt cgtgtggcgc
     4261 tgattccgtt ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa accctggtga
     4321 aagtgaaaga tgcggaagat caactgggtg cgcgcgtggg ctatattgaa ctggatctga
     4381 acagcggcaa aattctggaa tcttttcgtc cggaagaacg ttttccgatg atgagcacct
     4441 ttaaagtgct gctgtgcggt gcggttctga gccgtgtgga tgcgggccag gaacaactgg
     4501 gccgtcgtat tcattatagc cagaacgatc tggtggaata tagcccggtg accgaaaaac
     4561 atctgaccga tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc atgagcgata
     4621 acaccgcggc gaacctgctg ctgacgacca ttggcggtcc gaaagaactg accgcgtttc
     4681 tgcataacat gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa ctgaacgaag
     4741 cgattccgaa cgatgaacgt gataccacca tgccggcagc aatggcgacc accctgcgta
     4801 aactgctgac gggtgagctg ctgaccctgg caagccgcca gcaactgatt gattggatgg
     4861 aagcggataa agtggcgggt ccgctgctgc gtagcgcgct gccggctggc tggtttattg
     4921 cggataaaag cggtgcgggc gaacgtggca gccgtggcat tattgcggcg ctgggcccgg
     4981 atggtaaacc gagccgtatt gtggtgattt ataccaccgg cagccaggcg acgatggatg
     5041 aacgtaaccg tcagattgcg gaaattggcg cgagcctgat taaacattgg taaaccgata
     5101 caattaaagg ctccttttgg agcctttttt tttggacgac ccttgtcctt ttccgctgca
     5161 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga
     5221 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag
     5281 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct
     5341 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc
     5401 cggcgcgccg atctgaagat cagcagttca acctgttgat agtacgtact aagctctcat
     5461 gtttcacgta ctaagctctc atgtttaacg tactaagctc tcatgtttaa cgaactaaac
     5521 cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat gtttcacgta
     5581 ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa tagcctctaa
     5641 ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagcct ttaaggttta
     5701 acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga gcctctcaaa
     5761 gcaattttga gtgacacagg aacacttaac ggctgacatg gggcgcgccc aggtcagtca
     5821 cagtggagcc tagcactcac tcagcgtgac ggctcagagc agaattcacg agccagaaat
     5881 agtaactttt gcctaaatca caaattgcaa aatttaattg cttgcaaaag gtcacatgct
     5941 tataatcaac ttttttaaaa atttaaaata cttttttatt ttttattttt aaacataaat
     6001 gaaataattt atttattgtt tatgattacc gaaacataaa acctgctcaa gaaaaagaaa
     6061 ctgttttgtc cttggaaaaa aagcactacc taggagcggc caaaatgccg gcttacattt
     6121 tatgttagct ggtggactga cgccagaaaa tgttggtgat gcgcttagat taaatggcgt
     6181 tattggtgtt gatgtaagcg gaggtgtgga gacaaatggt gtaaaagact ctaacaaaat
     6241 agcaaatttc gtcaaaaatg ctaagaaata ggttattact gagtagtatt tatttaagta
     6301 ttgtttgtgc acttgcctgc aagccttttg aaaagcaagc ataaaagatc taaacataaa
     6361 atctgtaaaa taacaagatg taaagataat gctaaatcat ttggcttttt gattgattgt
     6421 acaggaaaat atacatcgca gggggttgac ttttaccatt tcaccgcaat ggaattaaac
     6481 ttgttgaaga gaatgttcac aggcgcatac gctacaatga cccgattctt gctagccgaa
     6541 ttccagtcag gctgctagca ccagagctac gtgaccgcag gactagctcc agctgagcga
     6601 cagcggcaag aaagccatcc acgccgaaaa ccccgcttcg gcggggtttt gccgcatctt
     6661 gccgtaactg acaaattaac cgaaggtttc ttccggccac tgggatgcaa tgaccttacc
     6721 aacaacgcta caggattcgt tgcacgggat cattgggtat tgcggattca gcggctgcag
     6781 gaaaacttgg ccagagtcgc ggatcagctt cttaaaggtg aattcatcac cacccaggcg
     6841 cgcaatgcaa aagtctcccg gctcaaccgc ctgctcagga tcaaccagga tcagcatacc
     6901 atccggaaat gacggcttgc tgcccgtcgg ggccgtcatg ctgttgcctt ctacttccag
     6961 ccaaaacgcg ctatcgctcg cttttttggt agtcgatacc caacgctcag cgtcaccttt
     7021 ggtgaaggta cgcagttccg ggctaaacat acctgcttga acgtggctga agaccgggta
     7081 ttcgtattca gagcgcagag acggttgcat gctaaccgct tcatacattt catagatttc
     7141 acgagctata gaaggagaga actcctcgac ggaaactttc agaatcttgg tcagcagtgc
     7201 ggcgttgtac gcgttcagcg cattaatacc gttaaacaga gcgccaacgc cgctctgacc
     7261 catgcccatc ttgtcggcta cgctttcctg agacagaccc agctcattct ttttcttctc
     7321 atagattgct ttcagacgac gtgcgtcctc tagctgctct tgtgtaagcg gctttttctt
     7381 cgttgacatt ttgatcctcc tttatatgga ggtactaaat cggaacgtta aatctatcac
     7441 cgcaagggat aaatatctaa caccgtgcgt gttgactatt ttacctctgg cggtgataat
     7501 ggttgcatca aatttgcgcg ccacattatt attcatacct ttgtggaccg tattacaaag
     7561 agaagtgtta aatcaaacaa aaaggaggat taatcatgga cacgtacgcg ggtgcttacg
     7621 accgtcagtc gcgcgagcgc gaaaattcga gcgcagcaag cccagcgaca cagcgtagcg
     7681 ccaacgaaga caaggcggcc gaccttcagc gcgaagtcga gcgcgacggg ggccggttca
     7741 ggttcgtcgg gcatttcagc gaagcgccgg gcacgtcggc gttcgggacg gcggagcgcc
     7801 cggagttcga acgcatcctg aacgaatgcc gcgccgggcg gctcaacatg atcattgtct
     7861 atgacgtgtc gcgcttctcg cgcctgaagg tcatggacgc gattccgatt gtctcggaat
     7921 tgctcgccct gggcgtgacg attgtttcca ctcaggaagg cgtcttccgg cagggaaacg
     7981 tcatggacct gattcacctg attatgcggc tcgacgcgtc gcacaaagaa tcttcgctga
     8041 agtcggcgaa gattctcgac acgaagaacc ttcagcgcga attgggcggg tacgtcggcg
     8101 ggaaggcgcc ttacggcttc gagcttgttt cggagacgaa ggagatcacg cgcaacggcc
     8161 gaatggtcaa tgtcgtcatc aacaagcttg cgcactcgac cactcccctt accggaccct
     8221 tcgagttcga gcccgacgta atccggtggt ggtggcgtga gatcaagacg cacaaacacc
     8281 ttcccttcaa gccgggcagt caagccgcca ttcacccggg cagcatcacg gggctttgta
     8341 agcgcatgga cgctgacgcc gtgccgaccc ggggcgagac gattgggaag aagaccgctt
     8401 caagcgcctg ggacccggca accgttatgc gaatccttcg ggacccgcgt attgcgggct
     8461 tcgccgctga ggtgatctac aagaagaagc cggacggcac gccgaccacg aagattgagg
     8521 gttaccgcat tcagcgcgac ccgatcacgc tccggccggt cgagcttgat tgcggaccga
     8581 tcatcgagcc cgctgagtgg tatgagcttc aggcgtggtt ggacggcagg gggcgcggca
     8641 aggggctttc ccgggggcaa gccattctgt ccgccatgga caagctgtac tgcgagtgtg
     8701 gcgccgtcat gacttcgaag cgcggggaag aatcgatcaa ggactcttac cgctgccgtc
     8761 gccggaaggt ggtcgacccg tccgcacctg ggcagcacga aggcacgtgc aacgtcagca
     8821 tggcggcact cgacaagttc gttgcggaac gcatcttcaa caagatcagg cacgccgaag
     8881 gcgacgaaga gacgttggcg cttctgtggg aagccgcccg acgcttcggc aagctcactg
     8941 aggcgcctga gaagagcggc gaacgggcga accttgttgc ggagcgcgcc gacgccctga
     9001 acgcccttga agagctgtac gaagaccgcg cggcaggcgc gtacgacgga cccgttggca
     9061 ggaagcactt ccggaagcaa caggcagcgc tgacgctccg gcagcaaggg gcggaagagc
     9121 ggcttgccga acttgaagcc gccgaagccc cgaagcttcc ccttgaccaa tggttccccg
     9181 aagacgccga cgctgacccg accggcccta agtcgtggtg ggggcgcgcg tcagtagacg
     9241 acaagcgcgt gttcgtcggg ctcttcgtag acaagatcgt tgtcacgaag tcgactacgg
     9301 gcagggggca gggaacgccc atcgagaagc gcgcttcgat cacgtgggcg aagccgccga
     9361 ccgacgacga cgaagacgac
//
