LOCUS       sequence-411144-       15196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BGC08 pathway. pT7-native CDS sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15196)
  AUTHORS   Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
  TITLE     Cross-kingdom expression of synthetic genetic elements promotes 
            discovery of metabolites in the human microbiome.
  JOURNAL   Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 
            10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
  PUBMED    35366417
REFERENCE   2  (bases 1 to 15196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.008"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1487-1505.e14"
COMMENT     Sequence Label: pPath-BGC08-pT7native
FEATURES             Location/Qualifiers
     source          1..15196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6688..6779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6780..6848,6849..7640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      7644..7683
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            7830..7938
                     /note="incP origin of transfer"
     rep_origin      7957..8345
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             10143..11960
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     promoter        complement(11961..11979)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11962..11979)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    11982..12015
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        12271..12614
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             12645..13454
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDVSD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12712..12731)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      13460..13657
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             13794..13811
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(13864..13882)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13878..13925
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     13944..13963
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13944..13962
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctcaagttt tgagcagatt taagggaagt aaataaatga agttcgatga agaatattac
       61 aaagaagtga taaaaaagta caattataat cctttattgt ttaataagat tgattttcaa
      121 ttgccaggta tttctaaaaa tgaaatgcaa ttaatgtggc tgtgtagtta ccattcttta
      181 aaaaatagga aagttaactg tgaaaatact ctatttacaa caggaatagg tctctcaggt
      241 gttccacata tgggtacaat atcacaaata attcgtgcta ttaagttaca acgaggaggc
      301 aataaagttc aaatagtgtt gggagatata gatgcgtata caggcagaca tatttcatgg
      361 acacaaacac agcatttaaa ggaaaaatat agaaatttta tattaaaatt aggtttcaat
      421 tctcaaacag gtatactaag agctcaaagt gataaaagtg tatttttgac tcaagcaaag
      481 attgcagggt attttaaaga tgaaattgct gatgaatctg aagaagattt gcacgactta
      541 tatgttaaag ataacaaagt tgaatctaaa atgacgttta atagaaaata ttcgttatct
      601 ttaatgtgtg cagattttat tgaaccatta ttaatggata attttacgaa tatagtagtt
      661 tttttaggac ttgatgaaca taaatacgtt agagcgtcaa aacaagttat ggatttaatg
      721 aaacaagaat ttaaagtttt agattccaaa aaaatatatg ctttatattc gcctttaatt
      781 agaggtttta atggctttcc taagatgtcg aaaagcttta cagaatcctc tttaagtatt
      841 caaaatacgc cttttgaaat agaacataaa attatgaata aaaaagaaga tgagatgcct
      901 cttcaactaa atgtaaacta tcaattaatg aaaggtttaa tggatatatc cgaagaaaaa
      961 gcaaagcagg ttcattcaga ttataaaaac caaaatgtag gatggattgt agataaagaa
     1021 gaattattaa aaagagtaat agaaattaga gatatgtggt atgaatgata aatttaacca
     1081 agttaactta attagtaaac agttacagca aattgtaaat gacaaaaaat atgacatttt
     1141 agaactaaag gatattcttt attatttaat gtcaagtgat aaaaaatatt cagatgcatt
     1201 cttaagttct ttattaatgg gattagaact tgtaaacatg ccgagtaaag acataaaaac
     1261 tttgattgat gtaataatta gaattaatca ttataatcct ttctcagttt ccttatctaa
     1321 atatcctaat attacttgcg tagcgggttc tggaaaaaaa tattataaaa ctttgaatat
     1381 tacaacttta gctacaattg tagcttcggg attaggagca gtaatcgtta aaccaacttc
     1441 caagggtgtt acttcacgtt taggttcaga agatttgctt gaatatttaa aacttccaat
     1501 aactaattct ataactaaag ctgaggcatt tatttctaaa aattctattg catttttttc
     1561 tatagaaaat ttgattccca agtttgatgc aatctatgga ggacgacagt taacaatatc
     1621 tccattaagt aatgctttgc cggggttaat gtcgcccata tcatgccaaa atatatattt
     1681 tggattaagt tctaattatc ataacaaggc tcttaattta ttgcgatcat atggaattgg
     1741 aggcaatata tcaatatcta gtacaaaatt aaacgaagga tatttagatg aattaacatt
     1801 tagcagtggt tctaagctga cttttgaatc ttctaataat tcaacaatta ttttagaagg
     1861 tattactttt gacaaagctg atttaaataa tgcaaggtca acaaataata taactaataa
     1921 tataaataaa attatttcag aaataaaaag tacagaagtt acggactata tgaaaatagt
     1981 atgcttgaac gctgctggta ttttgcttac tgcaggagtg tataaaaata tcagagtagc
     2041 gtatgaacaa gcaaagttat atgtacaaaa gggtaaatta ttagatagaa taaatgcatt
     2101 aaggggtaaa caattatgag tctatttgaa cagttattga atttaaagca gagtccaaaa
     2161 gtagttgtta aggattgtaa taatgattac tctgggaggc aattattaga aaaagtaggt
     2221 aaaattcaaa atgtactact acacctgggg gttaaaaatg gagataaaat tcctgtaata
     2281 gtggagcctt ctatagatgc aattgcttta gtgtatgcta ttggattctt gggtgccgta
     2341 tatgttccca tctcccctaa atcgcccaaa aaacgtatta attttattat aaaagatttg
     2401 agtgctaaat tggtacttac aaatcagaat tatctattgt tagataataa tataaatatg
     2461 tttacaataa aagatctact aagtatgaca atcaaggata aaacgcctac agttatgtct
     2521 agacgtcaaa atgatactgc ttatattatt tatacatctg gaagcacagg taaccctaaa
     2581 ggggtagaag tatcttttga taatttagag tcatttattc attctttcaa catttgtttc
     2641 ccaagttccc aaaattcaac ttacttatta aatacagctt tacaatttga cgtatcaatt
     2701 tctgaattat atggatggat ttctcaagga gcggttcttc aattactttc agcagaacaa
     2761 ttaaaagatt taaaaaactt accactttat gtaaagaaat atcatattac tcatttatgt
     2821 gcggcaccat ctacattaca agtcatggat aatagccgac tagatcttct tgacgagagt
     2881 agtttatcat tcctattaat tgcaggtgaa gaatttcctg tttctttggc aaagagactt
     2941 aaaaagcttg taaaaagtgg gaaagtatat aattgttatg gtccaacaga agcaacagtt
     3001 tatgcgttat atcataaagt tactatgaaa gacatattac aaggaaagat acctatcgga
     3061 cgcccatttc ctaacgctac tattaagttg ttacctcaga aaagaaaaaa ttttgaatta
     3121 cttattggtg gatatggagt gtccaaaggc tatttgaatt taaaaagaaa aaatagctac
     3181 agctttgttg agctagatgg agaaagattt tataaaactg gagatcttgt aaaatatgac
     3241 caagatatga ataatattat ttttgttgga agaaaagatt ttcaattaga aataaatagt
     3301 attcgggtag agccaagtga aattgaaggt gtaattaatc aagttgataa agtgattgac
     3361 agtttagttg tcatgaataa ggggatatta acgtgtattt acacaacgtt taagcattct
     3421 atagttgatt ctaacagtat taaactgaaa atttcagaaa aactaccgaa atatatgatt
     3481 cctaagcggt ggtacagagt ttcagagttt cctttaacta taaatggaaa aattgataga
     3541 aaaacgttgt taaagacctt ttctaatccc attaataatt ctgatactga agataaatta
     3601 ataagtaaaa ttgaacaaat ttgtgatatt tctaatttat ccgaaaatga caatattttt
     3661 gatcttggta ttgattcgtt gatgaccgtt gaaatagaaa ttatgctaga aaaacattac
     3721 cattcgaaaa taccaacagg ttttttatat gaacatccaa cagttgaaaa aataaaacgt
     3781 tattttaaag atgaatctaa gcttaaattc aataatattg aacaaatttt gaaaaagata
     3841 acagaacgtt acgtaattga taaagataaa ttgattatat attcaaatga taaaaatgtt
     3901 gaaaacaagt tgcaaaagca tctttataac ttatctttat tcaaagaaat taaaatcaat
     3961 gatatttcaa tttttaacaa cgaaaacaat tgtgaaacgg ttgttgatgg tggaaaaata
     4021 aacgaagata ggaactatga atcttcaata tttcaaaaag tatatactac tattaaactt
     4081 ccaagtttta ctgtagttag attttctatc caagatgatc tagtagggag taaaacaaaa
     4141 acattaatta ctcgattaat atatacaaca gaagcttttc gtacgaaaat tattaaaact
     4201 actgatggaa attatattag tacggtttat acagatctta atataagaag taacatcgaa
     4261 gatataacat atttgtctaa agaaaagcaa gttaattata taaataagaa aattggaaaa
     4321 tatgaaaacc aaattttatc taaattaaca agaaactatt tgtataaaat tttgtctttt
     4381 agaactagtt cccaaaatat tgaattagta attatttgtc atcataatat ttgtgatgga
     4441 gcaagcgaac aattgctaaa caagaaaatt gggcaactct taaataatca atcagtgact
     4501 gatttgaaga tgaaagatta tttagaaaca ataaaaatga attctgccat tgaaaacata
     4561 cttaatgatc ctgtaattaa agaaataggt aaaatgaaaa accagatagg taatgagaag
     4621 ctgacttgga gtgaaaaaag attagtagga gttgaaaaat taaataattt tcaaaaaatg
     4681 atgatggtag ccaattatgt tacaaaagaa tatttaaaag aaagcgggca tcaatttatg
     4741 acatttcaaa tgctgtttaa ttttagaaag gtaaataaca aagatttttc aactttgatt
     4801 aatgattgtc atgaaacatt aactttcttt agaaggaagt cagatacaga tattcagttt
     4861 gttaataatt tgttttatag attaaatgat tttcactata atcgtggaat aagtaccggt
     4921 tatgccattt ataataattt tcctgcattt aataatgaac agcttcaaat acaaaaaatt
     4981 ttggaagaag ctccaattaa tattgactat ataggtgaag tggaaacaaa gaacctagat
     5041 agtaaagcaa aagcgattat taatttgcga aagcaattaa aagatcttaa acatcaaatt
     5101 aggtttactg cattttctag caaaaatgag atgtttattt accgtatctc aggagaacag
     5161 acatggcaat attgaaaatt aatgtaagtg atactatccc tctatctcaa gatataacta
     5221 agagattaat aaatgttaat tatgtaggta tttgtggatc agataagcaa aaattagatg
     5281 atccacctat taaatattta ggacatgaaa taattggaaa ggatatggat aacggaaaat
     5341 ttgttgtaat aaatccaaat attacatgtg gtaaatgtta tagttgccta aatggttatg
     5401 aaaatttatg tagcaaagaa aaggctatag ggactaatgt gaatggtggt tttcaaggtt
     5461 ggttacgagt tccatctact aatctagaat atatagaaaa accagatatt aagtatatat
     5521 ttacagatcc tttagcagtt gttctgcatg gtatgtcaat gataaaaatt ttatctaatt
     5581 ataaagttat tgtattaggc aatggtagta tagcaaaaat attagtatgg cgattaaatt
     5641 tgttaggtat caagccagat gttatgtgca gatctggaat ttggaataat aaggaattaa
     5701 ccatatcgaa tttatataaa ttgacggaaa aagtatcgct taattactac gatatagctt
     5761 ttgagtgtat aggttttaaa cagtcggaaa ctatagaaaa atcattagga atgattagac
     5821 caaaaggaga atttatttgt ttcggtgttt ttccagagga ctattctcca acattaaaag
     5881 ttagaaggtt atttgaaaaa gaaataacta tattgggagt tcgatcattt acgaaaaaag
     5941 attttaaata cgccaaaaac atattagatc ttcatggtag taaattaata agtagattat
     6001 gtataagcgt tgtatctagt gaaaacataa atgctattat gaatgatata gaagatagaa
     6061 atattgataa agtaattgtt agaatgaagt gatagtttta aagattgcgt tttttgtaat
     6121 attatctcta agaatcttaa aagtaatatt attcatgaaa ataaaggtgc cattgttata
     6181 atgtcggata atcctgaagt agagggacat atgcttgttt ttgcaaaaaa gcattttcct
     6241 acattggaac tggcagatca gagttcttgg tactatattg gtgaaatatt aaatgaatta
     6301 ggaaagagac taaggtccca aggatatgat ggatataatt tattgagtgc taacggtgta
     6361 tctgcaggtc aatccgttaa acattttcac tttcatgtca ttccgagaaa gaagaatgat
     6421 ggcattaatg catggccaaa ttttgaaacg aaacctaata ataccagtga tttttttgaa
     6481 aaagtaaaat tttagagagc caccacagtg gttaattaag gcctgtgagg ccggaccaaa
     6541 acgaaaaaag gccccccttt cgggaggcct cttttctgga atttggtacc gaggggtgcc
     6601 agggcgtgcc ccagggctcc ccgggcgcgt ataatcgatt taaattagta gcccgcctaa
     6661 tgagcgggct tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt
     6721 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6781 tgagcattca gcattttcgt gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg
     6841 tgtttgcgca tccggaaacc ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc
     6901 gcgtgggcta tattgaactg gatctgaaca gcggcaaaat tctggaatct tttcgtccgg
     6961 aagaacgttt tccgatgatg agcaccttta aagtgctgct gtgcggtgcg gttctgagcc
     7021 gtgtggatgc gggccaggaa caactgggcc gtcgtattca ttatagccag aacgatctgg
     7081 tggaatatag cccggtgacc gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt
     7141 gcagcgcggc gattaccatg agcgataaca ccgcggcgaa cctgctgctg acgaccattg
     7201 gcggtccgaa agaactgacc gcgtttctgc ataacatggg cgatcatgtg acccgtctgg
     7261 atcgttggga accggaactg aacgaagcga ttccgaacga tgaacgtgat accaccatgc
     7321 cggcagcaat ggcgaccacc ctgcgtaaac tgctgacggg tgagctgctg accctggcaa
     7381 gccgccagca actgattgat tggatggaag cggataaagt ggcgggtccg ctgctgcgta
     7441 gcgcgctgcc ggctggctgg tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc
     7501 gtggcattat tgcggcgctg ggcccggatg gtaaaccgag ccgtattgtg gtgatttata
     7561 ccaccggcag ccaggcgacg atggatgaac gtaaccgtca gattgcggaa attggcgcga
     7621 gcctgattaa acattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt
     7681 ggacgaccct tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt
     7741 tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac
     7801 tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg
     7861 agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct
     7921 gctctgcgag gctggccgta ggccggccgg cgcgccgatc tgaagatcag cagttcaacc
     7981 tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg tttaacgtac
     8041 taagctctca tgtttaacga actaaaccct catggctaac gtactaagct ctcatggcta
     8101 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat aaaattaata
     8161 taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa aaagaatata
     8221 taaggctttt aaagccttta aggtttaacg gttgtggaca acaagccagg gatgtaacgc
     8281 actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac acttaacggc
     8341 tgacatgggg cgcgcccagg tcagtcacag tggagcctag cactcactca gcgtgacggc
     8401 tcagagcaga attcacgagc cagaaatagt aacttttgcc taaatcacaa attgcaaaat
     8461 ttaattgctt gcaaaaggtc acatgcttat aatcaacttt tttaaaaatt taaaatactt
     8521 ttttattttt tatttttaaa cataaatgaa ataatttatt tattgtttat gattaccgaa
     8581 acataaaacc tgctcaagaa aaagaaactg ttttgtcctt ggaaaaaaag cactacctag
     8641 gagcggccaa aatgccggct tacattttat gttagctggt ggactgacgc cagaaaatgt
     8701 tggtgatgcg cttagattaa atggcgttat tggtgttgat gtaagcggag gtgtggagac
     8761 aaatggtgta aaagactcta acaaaatagc aaatttcgtc aaaaatgcta agaaataggt
     8821 tattactgag tagtatttat ttaagtattg tttgtgcact tgcctgcaag ccttttgaaa
     8881 agcaagcata aaagatctaa acataaaatc tgtaaaataa caagatgtaa agataatgct
     8941 aaatcatttg gctttttgat tgattgtaca ggaaaatata catcgcaggg ggttgacttt
     9001 taccatttca ccgcaatgga attaaacttg ttgaagagaa tgttcacagg cgcatacgct
     9061 acaatgaccc gattcttgct agccgaattc cagtcaggct gctagcacca gagctacgtg
     9121 accgcaggac tagctccagc tgagcgacag cggcaagaaa gccatccacg ccgaaaaccc
     9181 cgcttcggcg gggttttgcc gcatcttgcc gtaactgaca aattaaccga aggtttcttc
     9241 cggccactgg gatgcaatga ccttaccaac aacgctacag gattcgttgc acgggatcat
     9301 tgggtattgc ggattcagcg gctgcaggaa aacttggcca gagtcgcgga tcagcttctt
     9361 aaaggtgaat tcatcaccac ccaggcgcgc aatgcaaaag tctcccggct caaccgcctg
     9421 ctcaggatca accaggatca gcataccatc cggaaatgac ggcttgctgc ccgtcggggc
     9481 cgtcatgctg ttgccttcta cttccagcca aaacgcgcta tcgctcgctt ttttggtagt
     9541 cgatacccaa cgctcagcgt cacctttggt gaaggtacgc agttccgggc taaacatacc
     9601 tgcttgaacg tggctgaaga ccgggtattc gtattcagag cgcagagacg gttgcatgct
     9661 aaccgcttca tacatttcat agatttcacg agctatagaa ggagagaact cctcgacgga
     9721 aactttcaga atcttggtca gcagtgcggc gttgtacgcg ttcagcgcat taataccgtt
     9781 aaacagagcg ccaacgccgc tctgacccat gcccatcttg tcggctacgc tttcctgaga
     9841 cagacccagc tcattctttt tcttctcata gattgctttc agacgacgtg cgtcctctag
     9901 ctgctcttgt gtaagcggct ttttcttcgt tgacattttg atcctccttt atatggaggt
     9961 actaaatcgg aacgttaaat ctatcaccgc aagggataaa tatctaacac cgtgcgtgtt
    10021 gactatttta cctctggcgg tgataatggt tgcatcaaat ttgcgcgcca cattattatt
    10081 catacctttg tggaccgtat tacaaagaga agtgttaaat caaacaaaaa ggaggattaa
    10141 tcatggacac gtacgcgggt gcttacgacc gtcagtcgcg cgagcgcgaa aattcgagcg
    10201 cagcaagccc agcgacacag cgtagcgcca acgaagacaa ggcggccgac cttcagcgcg
    10261 aagtcgagcg cgacgggggc cggttcaggt tcgtcgggca tttcagcgaa gcgccgggca
    10321 cgtcggcgtt cgggacggcg gagcgcccgg agttcgaacg catcctgaac gaatgccgcg
    10381 ccgggcggct caacatgatc attgtctatg acgtgtcgcg cttctcgcgc ctgaaggtca
    10441 tggacgcgat tccgattgtc tcggaattgc tcgccctggg cgtgacgatt gtttccactc
    10501 aggaaggcgt cttccggcag ggaaacgtca tggacctgat tcacctgatt atgcggctcg
    10561 acgcgtcgca caaagaatct tcgctgaagt cggcgaagat tctcgacacg aagaaccttc
    10621 agcgcgaatt gggcgggtac gtcggcggga aggcgcctta cggcttcgag cttgtttcgg
    10681 agacgaagga gatcacgcgc aacggccgaa tggtcaatgt cgtcatcaac aagcttgcgc
    10741 actcgaccac tccccttacc ggacccttcg agttcgagcc cgacgtaatc cggtggtggt
    10801 ggcgtgagat caagacgcac aaacaccttc ccttcaagcc gggcagtcaa gccgccattc
    10861 acccgggcag catcacgggg ctttgtaagc gcatggacgc tgacgccgtg ccgacccggg
    10921 gcgagacgat tgggaagaag accgcttcaa gcgcctggga cccggcaacc gttatgcgaa
    10981 tccttcggga cccgcgtatt gcgggcttcg ccgctgaggt gatctacaag aagaagccgg
    11041 acggcacgcc gaccacgaag attgagggtt accgcattca gcgcgacccg atcacgctcc
    11101 ggccggtcga gcttgattgc ggaccgatca tcgagcccgc tgagtggtat gagcttcagg
    11161 cgtggttgga cggcaggggg cgcggcaagg ggctttcccg ggggcaagcc attctgtccg
    11221 ccatggacaa gctgtactgc gagtgtggcg ccgtcatgac ttcgaagcgc ggggaagaat
    11281 cgatcaagga ctcttaccgc tgccgtcgcc ggaaggtggt cgacccgtcc gcacctgggc
    11341 agcacgaagg cacgtgcaac gtcagcatgg cggcactcga caagttcgtt gcggaacgca
    11401 tcttcaacaa gatcaggcac gccgaaggcg acgaagagac gttggcgctt ctgtgggaag
    11461 ccgcccgacg cttcggcaag ctcactgagg cgcctgagaa gagcggcgaa cgggcgaacc
    11521 ttgttgcgga gcgcgccgac gccctgaacg cccttgaaga gctgtacgaa gaccgcgcgg
    11581 caggcgcgta cgacggaccc gttggcagga agcacttccg gaagcaacag gcagcgctga
    11641 cgctccggca gcaaggggcg gaagagcggc ttgccgaact tgaagccgcc gaagccccga
    11701 agcttcccct tgaccaatgg ttccccgaag acgccgacgc tgacccgacc ggccctaagt
    11761 cgtggtgggg gcgcgcgtca gtagacgaca agcgcgtgtt cgtcgggctc ttcgtagaca
    11821 agatcgttgt cacgaagtcg actacgggca gggggcaggg aacgcccatc gagaagcgcg
    11881 cttcgatcac gtgggcgaag ccgccgaccg acgacgacga agacgacgcc caggacggca
    11941 cggaagacgt agcggcgtaa tctatagtgt cacctaaatg ggtgccaggg cgtgcccgtg
    12001 ggctccccgg gcgcgtatag ggataacagg gtaatacgtc aaattctatc ataattgtgg
    12061 tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa
    12121 ggctgttttc tgtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
    12181 ttataattag cttcttgggg tatctttaaa tactgtagaa aaagaaattg catacctttg
    12241 ttcctcggtt atatgtttgc tcatctgcaa gacatggagg cccagaatac cctccttgac
    12301 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
    12361 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
    12421 aattacggca cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
    12481 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
    12541 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
    12601 gaacataaac aaccaaaaat ttgtaattaa gaaggagtga ttacatgggt aaggaaaaga
    12661 ctcacgtttc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
    12721 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg
    12781 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
    12841 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
    12901 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggcaaa acagcattcc
    12961 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
    13021 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
    13081 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgtgagt gattttgatg
    13141 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat
    13201 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
    13261 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
    13321 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
    13381 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
    13441 atgagttttt ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt
    13501 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
    13561 tttttttcgc ctcgacatca tctgtccaga tgcgaagtta agtgcgcaga aagtaatatc
    13621 atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctc tagcataacc ccgcggggcc
    13681 tctttcgggg atctcgcggg gttttttgct gaaagaagct tcaaataaaa cgaaaggctc
    13741 agtcgaaaga ctgggccttt cgttatgttg ttgtcgctgc ggccgcactc gagcaccacc
    13801 accaccacca ctgggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
    13861 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
    13921 ttttgctgaa ggccatcatg gcctaatacg actcactata gggagatcct gcaggccaat
    13981 gtgatggaca ctaaaataga aaattaaaaa tttaggaagt ttaaaaatga aaaaagaaag
    14041 aaaaaatatt ttccttttgc ttagtgctaa ggtgatttct aatattggag ataatttttt
    14101 tgattttagt aataatactt ggatagcatc tctaggtgca aaaggtcaga aaatacttac
    14161 tatatatcaa ataacggaat caataaccgg tattgtatta aatgtgtttg gtggagtgtt
    14221 tgctgataac tataatagaa aaaaaattat taccataact gacttaatag ctggtgtagc
    14281 atgtttagtc ttaagtatag tatatcctac caaatttttt gtttttgcaa tgatattggt
    14341 aaatataata ctagctatac tttcatcttt tagatcaccg gcatataaat caattattcc
    14401 aaatacagtt cacaaagata aaatagtaaa agtaaattct aatttagaaa tttgtaatca
    14461 acttgttagt gttagttcgc cagtaatttc attgattgct tataaagttt ttgggatatg
    14521 tggagcactt ttgatagatg gaatatcttt tttaatatca gcacttctaa ataactgttt
    14581 acaagtaatc cataaaaaaa gtgacgaaaa tattaggcat ataaaaaata aaattattaa
    14641 gaacattatt gaaggtattg aatacattac taaaaattca caagtagcaa tattacttat
    14701 tgtctcatca gctgttaatt ttttcttagc tggatataat tactcttttc cttttgttaa
    14761 taaagctttt ggaacaggaa atagtacata tactatttta ttagtaagtc aatctttagg
    14821 tggaattatc ggaggaatta ttagtcccaa attgactaaa aacgattcta ttagtcattt
    14881 gcttaatcta ttaacatttt gtggattaag catcgcagga atctatttat tgtttcaatg
    14941 gtatcattcc ttattaacag tttttgtttt tatgttttta tttcatatgt tgctaacagc
    15001 atacaatatt gattttttta gcataatcca aacaaaagta gatgataagt atctagggag
    15061 agtgtttagt gtagtcttta ctgttgccat tatttttatg cctataggga cagccgtatt
    15121 ttcgtattat tcaaaagctt atgtggtaga aaattacata ataattggta ttgggataat
    15181 aatcgtttca attgta
//
