LOCUS       sequence-411162-       16773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 
            of PNRSV (Pch12 isolate) in transfected cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16773)
  AUTHORS   Cui H, Wang A
  TITLE     An efficient viral vector for functional genomic studies of Prunus 
            fruit trees and its induced resistance to Plum pox virus via 
            silencing of a host factor gene.
  JOURNAL   Plant Biotechnol J. 2016 Aug 27. doi: 10.1111/pbi.12629.
  PUBMED    27565765
REFERENCE   2  (bases 1 to 16773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2016 Aug 27. doi: 10.1111/pbi.12629."
COMMENT     Sequence Label: pCaRNA1&2
FEATURES             Location/Qualifiers
     source          1..16773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..36
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(51..56,57..74,75..81)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    89..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     94..116
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     113..129
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     113..129
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        538..583
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_RNA        3939..3990
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     polyA_signal    4063..4239
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(4248..4264)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5044..5089
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_RNA        7704..7755
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     polyA_signal    7828..8004
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(8013..8029)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8232..8256
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9557..10186
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10615..11688
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11754..11948
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12184..12206)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12292..12432
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12347..12366
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12447..12464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12618..13206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12698..12717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13293..14087)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    14512..14536
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    14614..14788
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(14829..15854)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(15921..16597)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
       61 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      121 agctatgaca tgattacgaa ttctgaagct tgcatgcctg caggtcaaca tggtggagca
      181 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc agagggctat
      241 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
      301 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
      361 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
      421 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      481 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      541 agacccttcc tctatataag gaagttcatt tcatttggag agggttttta gttcgtggtt
      601 gaactattta ttatggatcc agaatctcta tcacagttgc ttgtttctac cttacagaaa
      661 caatgcgctg atgaaacctc gacagtgggg aaggtattct ccgagagagg tgtagctctg
      721 gttgagcgat ctattccgaa ggccgaaggt gaacgactta atataagttt tgccctgagt
      781 gccgagcaga agtcattgtt gtctcgaaat tttcctggta gagacataca ttttgcacaa
      841 cgagactctt cgagtcatag ttttgcagcg gctcataggt tgttagaaac cgattatatt
      901 tataagtgct tcggcacgac tgaagaggcg gtgattgatc ttgggggaaa tttcgtttct
      961 cacataaagc agaaaaggta taatgtgcat agctgttgtc cgcttctaga tgaccgcgat
     1021 ggtgccagat ttaccgaacg tttgatctca ttgaagacct acttgcgaac tcacaaggag
     1081 gaaagacacg aggcagacta ttgcgaatgc cgcttcgagg agtgcccgag acgggccgac
     1141 tatgtaatgg ccgtgcacgc tgttagcgac ttgcctatca cagatctttg tgccgcattg
     1201 actaggaaag ggacaaagaa gatgattttg tccataatga tggatccgaa tatgttgttg
     1261 agggatgttg gtgaaatacc gaacttcaac gttcgttggg agatagacag aaccgaggat
     1321 atcataaggt tcgattttat cgatgccccg tgtttgggat accaacacaa gttttctgta
     1381 ttacagcaat atctcaccac gaatgctgtt atagtgggag ataaagcagc ttatcgcgtt
     1441 gagcgaaaaa gcgatttcgg cggtgtgttc attgtggata taacggccgt tgctgggtac
     1501 aagccgggca tggtagtggg tggtactaga tcttgcgcgt ggtctactct aatccgaaac
     1561 aaaaccgttg ttcatactgt cgacggtgag gatcattggt ggtatgatgt tactcgcaga
     1621 tcgaagatcc ttgtggacac taaggtattg acgaaagtgt tggaagcaag tttccgtcaa
     1681 tttaaaccga atgtggagcc tgagtcgatg attcagaaca tcgcgacgat gttgtcttct
     1741 tcgacgaatt acactgtgat taacggtgtt acactccaag caggtgagag tctaccttat
     1801 ggcgattatg tagccatagc caccactatt tacgtgagga ccaagaggat gtatgattcg
     1861 ataagaacca acatcgatag attaaacgaa acccgatcag tcccgataaa ggacgtagca
     1921 cagggtgaag tttacggtaa gttgggtaat gctttaggca gtttgtttgc gccgactgcg
     1981 actacccaag ttattgcagg tccgaaaggt agagaggatg tttatgagtt gcatccgaag
     2041 tcttggtctg acgaagttag aaattttttc tattgttgtg ttggtagaca aggagtcgga
     2101 catactctac tgagcgaccc ggatttgttc gtaccactcg aagttgtttt acagaccaag
     2161 tggactggtg gttcggtctt gactgtaaaa actctgtttg acgatgtagt caaaccagaa
     2221 tacgatagag tggaagagga acttcagcgc gtgcgagatg acagagataa gtcagagaaa
     2281 ctacagaaag cgatcttgac cgtcgcgaag tggatagaag cccatcccga tggaaagtta
     2341 cccaagggtt taggcgaggt agcagcgcta gttccagata tggtagattt gcgtgagtcc
     2401 gtcgtagctg tcgaaccaaa ggccgttgtg aataagtatg catctgaaat tcaagaagct
     2461 attacttatt acgagctaga agctgacatt tccgcgaaga aattgaggtc tgtgggtgaa
     2521 cactgtaatt ggtcgaagaa atttatttcg actatttggg cgggcgatga gtcccggcgt
     2581 gtgtatattc cacagaccga cagttggtta ggaccaccca atgttccgaa accagaaccg
     2641 actggtcaat acgaaagggg gatgactgct gatgggtatg tgctcatggc ctggagtggt
     2701 tccagtggtc aaaaatttga cggtccatta caagtcgacg ctgtgtgtcg tagagaattg
     2761 tcgcgttacc ccgcgatatt tttcgataag tcgtgcgagt tcgccgccaa cttaaggttg
     2821 ttaccggcat tacgagaagc actcactgta gaggcgaagt ttacgcggaa gttgatggat
     2881 ggcgtagctg gatgtggaaa aaccacgaag atcctcaacg aatgtaagat gtgtaacgat
     2941 cagcctgatc ttgttttgac tagcaacagg tcctcggcga tggagctgag ggagaaacta
     3001 ccaggtagtc aattattacg atccactcga gttaggacgt cagactcata tttgatgaat
     3061 ccgaagagac cttcatcggt tcgagtgatt tttgacgagt gtttcttaca acacgctggt
     3121 tgcgtttacg cggctgctag tctagcagga gctgaggagt taatattgtt tggtgatacg
     3181 aaacaaattc cctttgtatc aaggattcct catttccgat taaaggacca tttagtgtcg
     3241 gctgacgaaa aggtgatgtc gaatttgact taccgatgtc ctgctgacgc cactatggct
     3301 ctttcgaaat ggttttaccg aaggaacgtg aaaacagcaa acaccacact taggagtatg
     3361 agtgttaagc cgattgtgtc tgtcagtcaa atcgaccggg actttgattt gtatatgacg
     3421 catacacagg cggagaaaca cacactgata gccagtggtg tagttccgag agataaggtt
     3481 ttcacttcag ctgaagcaca agggaaaacc gagggtagag ccgcgctagt tcgattgtca
     3541 agaactagta tgagtctgtt tactggtaag gacccgctga tgggtccttg tcatagtttg
     3601 gtagctctgt ctcgatttaa gagacagttt gtttacttca ctgtggctga tacggattca
     3661 gacgatttga ttgctaaggc aatcagagat gttagtaaca gtagtgacga tgcagtcagt
     3721 agttacatac atcgagatca taaaatttag ggtgagatga acacacccga ttcccagatt
     3781 atggaagaac cgtaccaccg tgtggattta tcggttatct cttttgaagt tgtttcctta
     3841 cttcagtgtt ctgagagatt acctgcgttt gcagatgcct acaacgagaa gttgtagatg
     3901 ccccgttagg gaagcggtac ctctagagga tccgataccc tgtcaccgga tgtgttttcc
     3961 ggtctgatga gtccgtgagg acgaaacagg actgtcctgc aggagctcga attcggtacg
     4021 ctgaaatcac cagtctctct ctacaaatct atctctctct attttctcca taaataatgt
     4081 gtgagtagtt tcccgataag ggaaattagg gttcttatag ggtttcgctc atgtgttgag
     4141 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     4201 taattcctaa aaccaaaatc cagtactaaa atccagatca gcttggcact ggccgtcgtt
     4261 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct aagcttgcat
     4321 gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
     4381 cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
     4441 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     4501 atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctcta
     4561 ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa aaagaagacg
     4621 ttccaaccac gtcttcaaag caagtggatt gatgtgatgc cgcctgcagg tcaacatggt
     4681 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
     4741 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     4801 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
     4861 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
     4921 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     4981 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     5041 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagaggg tttttgtact
     5101 cgtggttgag ttacaatgaa tcctctaaat cagctattgt cacatgggtg ttctgctatt
     5161 tcctcagctt tacatagaga tgttgcttac acccagatat ctgatgagtg ttttgattct
     5221 catttcactc agatttccac cgaatacttt cttactactt ccgctaggaa attgtttgaa
     5281 gcgtacatgg tgacgatgtt tcttaagaag catatgagtt atgtcttcga ggactttata
     5341 ggatccgaga tgggtccgta cgtccagacc agatggcgga tcgtttttga tctggacgag
     5401 gtcgaagaag aatatgtcta caaacccgac ttctatcatt acgatgttta tgattacgaa
     5461 atgggtgtgg atatacctgc atcgataatg gacgatattc cattctgtga agatgcagaa
     5521 gcagtgacgt gtcgcaattg tggtagtttc gatgagatag aaacggtgta ccaagcagcg
     5581 acgtcttctg acgagtcatg taccaacgtt tgtgatgttg atgcccctca cgataccatc
     5641 aagtggtctg acgaacccga tttgatggtt gatgacccat cgaagttgca cgatttggac
     5701 gatgaagttc cgccgttgca attagagact gttggtcggg tagtgcagtc cgtggagcct
     5761 ttggtcccgg acattaactc gttcgagacg aatcagttgc acttagtgaa ggaagtggta
     5821 ttggacagat ttgagtccat acttgaatgt cacgaaaagg tattggctag aaacgattgg
     5881 aagtctctag ctatgtatgt accgactggt gagattccat cagttagtga catattggaa
     5941 attccggtaa aacacacgca accggagttc attcagatgg caatagatga gatgttgcca
     6001 ggagtttcag atcttgatga taggtttttc caagaaatag ttgaaacgtc tgacattaat
     6061 ctggagatag acagagcatc tatcgatatg tcagtgttca acgactggac tccgaagtcg
     6121 ggtaggctga atggactgtt ccaaacaggt aatatctcga aaagagtacc gactttccgc
     6181 gaagcgtctc tcgctattaa gaagcggaat ttgaatgtcc ctgatttgca gcaagttttg
     6241 catgaggacg atgaagctag attaatagct aataagttca taaacaccgt tatcgatccg
     6301 aataaattgg ctcagtttcc aggatacata tccgaaggtg aaatgggtta tttcaataag
     6361 tatctatcag ggaaagctgt acccgatgat gcttttgtgg atccatgtgc gttggtctcg
     6421 atggacaagt atcgacatat gattaagaca caacttaaac ctgtcgaaga tacgtctcaa
     6481 ttgttcgaga gacctttagc ggcaactatt acttatcatg acaaaggaaa ggttatgtca
     6541 acatcaccga tattcctgat gatgtgtaat cgtttgctgc tgtgtttgaa cgataagatt
     6601 tccattcctt cgggaaaaca tcatcagttg ttctcgttgg atccctttgc gtttgaaatg
     6661 acgaaagagt ttaaagagat tgatttttcg aaattcgata agtctcagca aaggttacat
     6721 cacctgattc aatttcacat tttcaccgct ttaggtgctc cgaaagattt cctcgatatg
     6781 tggtttggat cacacgagat atcgcacatc cgtgatggac cttgtggtat cggatttagt
     6841 gtgaattacc agaggcgtac aggagatgca tgtacgtatt tgggtaatac tattattact
     6901 ttgtctgccc tggcgtacat gtatgacttg ctggatccga acgtgacgtt tgtcatagct
     6961 tcaggagacg atagtcttat aggttcaatt aaacctctgg accgatctga agagtttaaa
     7021 tttgtcacgt tgttcaattt tgaagcgaaa tttccacata accaaccttt tgtgtgcagt
     7081 aaatttttat gtttactgcc tactgtgagt gggggaaaga aggttctggc agttccgaat
     7141 gctttaaagt tgaatataaa actaggtgtt aaggatctgg caccatgtgt ttttgatgct
     7201 tggtataaat cctggttgga tttaatttgg tacttcgaca attacctggt tgtgtcgact
     7261 atgaaagact atatcagcca taggtattta agaagacaga catgttacca agaaggtgcg
     7321 atgttggctt ataggacgat attctcgagt aaagagaaat gtttgaagtc cttgtttggt
     7381 atcacttcca aagatctgga agcgatgttg cccatgagta agaatgggaa aggctctaat
     7441 agagccgagt gttcaaagaa taacaaaaca tctagacctc cgaagagtac tagatgtagg
     7501 aattctgcga actagaacga gttgaacgcg ttcgaatttc ctcgaataga gaagaatcgt
     7561 accaccgtgt gggtttgcga ttagttccta gagagttgct tccggctctc gagttcatag
     7621 gtacttacct gctttgcaga tgcctacgac gtgaagtcgt agatgccccg atagggaagc
     7681 ggtacctcta gaggatccga taccctgtca ccggatgtgt tttccggtct gatgagtccg
     7741 tgaggacgaa acaggactgt cctgcaggag ctcgaattcg gtacgctgaa atcaccagtc
     7801 tctctctaca aatctatctc tctctatttt ctccataaat aatgtgtgag tagtttcccg
     7861 ataagggaaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     7921 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     7981 aaatccagta ctaaaatcca gatcagcttg gcactggccg tcgttttaca acgtcgtgac
     8041 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     8101 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     8161 ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa
     8221 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     8281 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     8341 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     8401 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     8461 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     8521 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     8581 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     8641 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     8701 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     8761 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     8821 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     8881 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     8941 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     9001 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     9061 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     9121 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     9181 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     9241 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     9301 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     9361 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     9421 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     9481 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     9541 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     9601 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     9661 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     9721 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     9781 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     9841 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     9901 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     9961 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
    10021 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
    10081 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
    10141 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
    10201 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
    10261 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
    10321 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
    10381 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
    10441 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
    10501 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
    10561 ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
    10621 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
    10681 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
    10741 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
    10801 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
    10861 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
    10921 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
    10981 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
    11041 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
    11101 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
    11161 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
    11221 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
    11281 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
    11341 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
    11401 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
    11461 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
    11521 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
    11581 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
    11641 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
    11701 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
    11761 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
    11821 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
    11881 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
    11941 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
    12001 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
    12061 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
    12121 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
    12181 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    12241 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
    12301 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
    12361 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    12421 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    12481 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    12541 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    12601 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    12661 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    12721 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    12781 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    12841 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    12901 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    12961 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    13021 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    13081 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    13141 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    13201 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    13261 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
    13321 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    13381 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    13441 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    13501 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    13561 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    13621 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    13681 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    13741 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    13801 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    13861 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    13921 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    13981 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    14041 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    14101 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    14161 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    14221 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
    14281 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
    14341 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
    14401 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
    14461 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
    14521 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
    14581 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
    14641 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
    14701 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
    14761 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg
    14821 catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
    14881 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
    14941 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
    15001 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
    15061 tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
    15121 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
    15181 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
    15241 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
    15301 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
    15361 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
    15421 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
    15481 gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt
    15541 tcaggcaggt cttgcaacgt gacaccctgt gaacggcggg agatgcaata ggtcaggctc
    15601 tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
    15661 cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
    15721 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
    15781 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
    15841 tcaggctttt tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt
    15901 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
    15961 gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata
    16021 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
    16081 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
    16141 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
    16201 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
    16261 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
    16321 acgagagtgt cgtgctccac catgttcaca tcaatccact tgctttgaag acgtggttgg
    16381 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
    16441 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
    16501 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
    16561 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
    16621 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg
    16681 ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    16741 cacgacaggt ttcccgactg gaaagcgggc agt
//
