LOCUS       sequence-411173-       11953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial double
            HA tag in domain II and a mutation in the P loop of Domain I which 
            reduces trafficking to the cell surface.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11953)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 11953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: Cav2.2 HA EIA (E314A) pCAGGS
FEATURES             Location/Qualifiers
     source          1..11953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          625..1641
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1565..1587
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1649..1668
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3435..3461
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3465..3491
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(8971..8990)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9036..9091
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9090..9109)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9452..9468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9452..9468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9465..9487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9476..9492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9500..9506,9507..9524,9525..9530))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9545..9566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9625..9820
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9671..9806
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9826..9960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9863..9882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9917..9936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10028..10045)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10199..10787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10279..10298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10958..11749,11750..11818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11581..11600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11819..11923)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggggcgsgn gcsaggcgga gaggtgcggc ggcagccaat
      541 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
      601 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
      661 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
      721 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
      781 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
      841 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc
      901 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt
      961 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg
     1021 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg
     1081 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
     1141 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
     1201 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg
     1261 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
     1321 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
     1381 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
     1441 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     1501 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     1561 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     1621 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     1681 tttggcaaag aattcctcga gggtaccggg ccccccctcg aggtcgaagc ttgatcatgg
     1741 tccgcttcgg ggacgagctg ggcggccgct atgggggcgc gggcggcgcg gagcgggccc
     1801 ggggcggcgg ggccggcggg gctggcggcc cgggccccgg ggggctgccg ccgggccagc
     1861 gggtcctcta caaacagtcg atcgcgcagc gcgcgcggac catggcgctg tacaacccca
     1921 tcccggtcaa gcagaactgc ttcaccgtca accgctcgct cttcgtcttc agcgaggaca
     1981 acgtcgtccg caaatacgcc aagcgcatca ccgagtggcc tccgttcgag tacatgatcc
     2041 tggccaccat catcgccaac tgcatcgtgc tggccctgga gcagcacctc cctgacgggg
     2101 acaagacgcc tatgtccgag cgcctggatg acaccgagcc ctacttcatt gggatcttct
     2161 gcttcgaggc ggggatcaag atcctggccc tgggctttgt tctgcacaag ggctcctatc
     2221 tgcggaacgg ctggaacgtc atggacttcg tggtagtcct cacggggatt cttgccacag
     2281 ccggaaccga cttcgacctg cgaacgctga gggctgtgcg tgtgctaagg cccctgaagc
     2341 tggtgtctgg gattccgagc ttgcaagtgg tgctcaagtc catcatgaag gccatggtcc
     2401 cgctgctgca gatcgggctg ctgctcttct tcgccatcct catgtttgcc atcatcggcc
     2461 tcgagttcta catgggcaag ttccacaagg cctgcttccc caacagcaca gacccagatc
     2521 ccgtgggtga cttcccctgt ggcaaggagg ccccggcccg gctctgtgag ggagacacgg
     2581 agtgccggga gtactgggcc ggacccaact ttggtatcac caactttgac aacatcctgt
     2641 ttgccatctt gaccgtgttc cagtgcatca ccatggcggg ctggactgac atcctctaca
     2701 atacaaacga tgcagccggc aacacgtgga actggctcta cttcatccct ctcatcatca
     2761 tcggctcctt cttcatgctc aacctggtgc tgggtgtgct ctcaggagag tttgctaagg
     2821 agcgcgagag agtggagaac cgccgcgcct tcctgaagct ccggcggcag cagcagattg
     2881 agcgggagct caacgggtac ctggagtgga tcttcaaggc tgaggaagtc atgctggctg
     2941 aggaggacag gaacgcagag gagaagtccc ctctggatgc agtgttgaag agagcagctg
     3001 ccaagaagag cagaagcgac ctgatccagg ccgaggaggg ggagggccgg ctcacaggcc
     3061 tctgtgctcc cggttccccg ttcgcccgtg ccagcctgaa aagcgggaag acggagagct
     3121 cgtcgtactt ccggaggaag gagaagatgt tccgcttctt catccggcgc atggtgaagg
     3181 cacagagctt ctactggacg gtgctgtgtg tggtggcact gaacacactg tgcgtggcca
     3241 tggtgcacta caaccagccg cagcggctca ccacggcatt gtactttgca gagtttgttt
     3301 tcctgggtct cttcctcaca gagatgtccc tgaagatgta tggcctgggg cccaggagct
     3361 acttccggtc ctccttcaac tgttttgact ttggggtcat cgtgggcagt atctttgaag
     3421 tggtctgggc tgcttacccc tacgacgtcc ccgactacgc cggctacccg tacgacgtcc
     3481 cggactacgc cggcgtcgac gtcaagccag ggacctcctt cgggatcagt gttctgcggg
     3541 ctctgcgctt actgagaatc ttcaaggtca ccaaatactg gaactcccta cggaacctgg
     3601 tggtgtctct gctcaactcc atgaagtcca tcatcagcct cctcttcctg ctcttcctgt
     3661 tcattgttgt ctttgctctg ctggggatgc agctgtttgg aggccagttc aactttaaag
     3721 acgagacccc aacgaccaac tttgacacct tccctgctgc tatcctcacc gtcttccaga
     3781 tcctgaccgg agaggactgg aatgcggtca tgtaccacgg gatcgagtcg caaggtggcg
     3841 tcagcagagg aatgttctcc tccttctact tcattgtcct cactctgttt gggaactaca
     3901 ccctgctgaa tgtcttcctg gccattgctg tggacaacct tgccaatgct caggagctga
     3961 ccaaggatga agaggagatg gaagaagcag ccaatcagaa actcgccttg cagaaggcca
     4021 aggaagtggc tgaagtcagc cccatgtctg ctgcgaacat ctccattgcc gccaggcagc
     4081 agaactcggc caaagcgcgc tcggtgtggg agcagcgagc cagccagctg cggctgcaga
     4141 acctgagggc cagctgcgag gcgctgtaca gcgagatgga ccccgaggag aggctgcgct
     4201 acgccaccgc gcgccacctg cggcccgacg tgaagacgca cctggaccgg ccgctggtgg
     4261 ttgagcccgg ccgcgacgct ccgcggggcc ccccgggggg gaagtcccgg ccggatggct
     4321 cggaggcccc cgagggtgcg gatcccccgc gcaggcatca ccggcaccga gacaaggaca
     4381 aggctcccgc cacggtcccc tcggcggggg aacaggacag agccgaagct ctgagggcag
     4441 agggcgggga gctggggccc cgcgaggagc gggggcggcc acgccgcagc cgcagcaagg
     4501 aggccccggg cgccccggag gtgcgcagcg atcgcggccg tggcccgtgt cctgaaggtg
     4561 gtcggcgcca ccaccggcgc gggtcaccgg aggaggcagc tgagagggag ccccgccgcc
     4621 accgcgcgca ccggcacggg cccgacccgg gcaaggaggg cccagcgtcc gggaccaggg
     4681 gcgagcggcg cgcacgacac cgcacgggcc cccgggcgtg cccccgggag gctgagagca
     4741 gcgaggagcc tgcgcggcgg caccgggctc ggcacaaggc gccgcccacc caggagaccg
     4801 cggagaagga caaggaggct gccgagaagg ggggcgaggc cacggaggcc gagaaggaca
     4861 aggaggctcg gaaccaccag cccaaggagc tgccctgtga cctggaggcc atcggcatgc
     4921 tgggagtggg cgctgtgcat acgctgccca gcacctgtct gcagaaggtg gaggagcagc
     4981 cagaggatgc ggacaaccag cggaacgtca cccgcatggg tagtcagccc ccggacacca
     5041 gcaccactgt gcacattcct gtgacgctga ccggcccccc cggggagacc acggtagtgc
     5101 ccagtggaaa cgtggacctg gaaagtcaag cagaggggaa gaaggaggtg gaaaccagcg
     5161 atgtgatgag gagcggccct cggcccattg tcccgtacag ttccatgttc tgtttaagcc
     5221 caaccaacct gctgcgccgc tgctgccact acattgtgac catgcgatac ttcgagatgg
     5281 tcatcctcgt ggtcatcgcc ctgagcagca tcgccctggc tgccgaggac cccgtgagaa
     5341 cagactctcc caggaataat gctctgaagt acatggacta catcttcaca ggcgtcttca
     5401 ccttcgagat ggtgataaag atgatcgatt tgggactgct gctacaccct ggagcctatt
     5461 tccgggatct gtggaacatt ctggacttca ttgtggtcag tggggccctg gtggcatttg
     5521 ctttctcagg atccaaaggg aaagacatca gcaccatcaa atccctgaga gtcctgcgtg
     5581 tgctgcggcc actcaagacc atcaagcggc tgcccaagct caaggccgtg tttgactgtg
     5641 tggtgaactc actgaaaaac gtcctcaaca tcctgatcgt ctacatgctc ttcatgttca
     5701 tctttgccgt cattgccgtg cagctcttca aagggaagtt cttctactgc acggacgaat
     5761 ccaaggagct ggagcgtgac tgcaggggtc agtatttgga ttatgagaag gaggaagtgg
     5821 aagctcaacc gaggcagtgg aagaaatatg acttccacta tgacaacgtg ctctgggctc
     5881 tgctaacgct cttcacggtg tccacagggg aagggtggcc catggtgctg aaacactctg
     5941 tggatgccac ctacgaggag cagggcccga gccccgggta ccgcatggag ctctccatct
     6001 tctacgtggt ctactttgtg gtctttccct tcttctttgt caacatcttc gtggccttga
     6061 tcatcatcac cttccaggag cagggggaca aggtgatgtc cgagtgcagc ctggagaaga
     6121 acgagagggc ttgcatcgac tttgccatca gcgccagacc cctgacgcgg tacatgcccc
     6181 agaacaagca gtcgttccag tataagacgt ggacgttcgt ggtgtcccca ccttttgagt
     6241 acttcatcat ggctatgatc gccctcaaca ctgtggtgct gatgatgaag ttctacgatg
     6301 cgccgtacga gtatgagcta atgctgaaat gcctgaacat cgtgttcacg tccatgttct
     6361 ccatggagtg tgtgctgaag atcatcgcct tcggggtgct gaactatttc agagatgcct
     6421 ggaatgtctt tgactttgtc acggtgttgg gaagtattac tgatattttg gtaacagaga
     6481 ttgcgaacaa cttcatcaac ctcagcttcc tgcgcctgtt ccgggccgcg cggctcatca
     6541 agctgctgcg ccagggctac accatccgca tcctgctttg gaccttcgtg cagtccttca
     6601 aggcgctgcc ctacgtgtgt ctcctcatcg ccatgctctt cttcatctac gccatcattg
     6661 gcatgcaggt ttttgggaac atcgccctgg acgatgacac cagcatcaac aggcacaata
     6721 acttccggac gtttctgcag gccttgatgt tgctgttcag gagcgccacg ggggaggcct
     6781 ggcacgagat aatgctgtcg tgcctcagca gccgggcctg cgacgagcac tccaatgcca
     6841 gcgagtgcgg cagcgacttt gcctactttt acttcgtctc cttcatcttc ctctgctcct
     6901 ttctgatgtt gaacctcttt gtggctgtga tcatggacaa ttttgagtac cttacacggg
     6961 actcttccat cttagggcct caccacctgg atgagttcat ccgggtctgg gctgaatacg
     7021 acccggccgc gtgtgggcgc atcagttaca gtgacatgtt tgagatgctg aagcacatgt
     7081 ccccacccct ggggctgggg aagaagtgcc ctgctcgagt cgcgtacaag cgcctggtcc
     7141 gcatgaacat gcccatctcc agtgaggaca tgacggtgca cttcacatcc acgctgatgg
     7201 ctctcattcg gacagcgctg gacatcaagc tggcaccagc tgggaccaag cagcaccagt
     7261 gcgacgcaga gctgaggaag gagatttcct gtgtgtgggc caatctgccc cagaagaccc
     7321 tggacttact ggtaccaccc cataagcctg acgagatgac ggtgggcaaa gtctatgcag
     7381 ctctgatgat cttcgacttc tacaaacaga acaaaaccag cagagaccag actcagcaag
     7441 cccccggagg cctgtcccag ctgggccccg tgtccctgtt ccacccgctg aaggccacgc
     7501 tggagcagac tcagccggcg ctgcgtggag cccgggcttt cctgcggcag aagagttctg
     7561 cctccctgag caacggcggg gccgtacaaa cccaggagag cggcatcaag gaatcggttt
     7621 cttggggcac tcagaggacc caggatgtgc tttgcgaggc cagggcaccc ctggagcggg
     7681 gccactcagc tgagatccca gtcgggcagc ctgggacact ggctgtagat gtccagatgc
     7741 agaacatgac tctgagtggc cctgacgccg agccgcagcc agggctggag agccagggcc
     7801 gagccgcctc catgccccgc ctggccgcag aaacacagcc cgcgcctgac gccagcccca
     7861 tgaagcgctc catctccacg ctggccccgc gcccccacac ggcccggctc ggcagcaccg
     7921 cgctggaccg cccagccccc agccaggccc cgcaccatca ccaccaccgc tgccaccgcc
     7981 gcagggacag gaagcagcgg tccctggaga agggtcccag cctgtctgct gacacagatg
     8041 gggcccccga cagcacggtg gggccaggac tgcccacggg ggagggcccc cccggctgca
     8101 ggcgggagcg ggagcgcagg caggagcggg gccggtccca ggagcggagg cagccctcgt
     8161 cctcctcctc ggagaagcat cgcttctact cctgtgaccg cttcgggggc cgcgaacccc
     8221 cacagcccaa gccctccctc agtagtcacc ccacgtcgcc gacggccggg caggagccgg
     8281 gaccccaccc gcaggggagt ggttcggttc acgggagccc cctgctgtcc acatctggtg
     8341 ctagcacccc gggccggggg cggaggcagc tcccccagac ccccttgacc ccccgcccca
     8401 gcgtcaccta caagacggcc aactcctcgc ctgtccactt cgccggggca ccgagcggcc
     8461 tccccgcctt ctcccccggc cggctcagcc ggggcctctc agaacacaac gccctgctgc
     8521 agagggaccc gctcagccgg cccctggcgc ccggctcgcg catcggctcc gacccctacc
     8581 tggggcagcg gctggacagc gaggcgcccg cccgtgccct gcccgaggac gcgcccgcct
     8641 ttgaggagac cgcggccagc aactcgggcc gctcctcccg gacctcgtac gtgtcctcac
     8701 tgacctcgca gcccccgccg ctgcgccgcg tgcccaacgg ctaccactgc actctggggc
     8761 tgggcggggg cggccgggcg cggcgcggct gccaccaccc cgaccgcgac cgccggtgct
     8821 agactagttc tagcaagctt cgacgaattc gcatgcggcc gctagctcga ggtaccgata
     8881 tcgagctcaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     8941 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     9001 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     9061 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     9121 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     9181 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     9241 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     9301 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     9361 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     9421 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9481 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9541 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9601 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
     9661 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9721 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9781 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     9841 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9901 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9961 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    10021 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    10081 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    10141 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    10201 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    10261 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    10321 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10381 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10441 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10501 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10561 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10621 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10681 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10741 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10801 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10861 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10921 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10981 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    11041 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    11101 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    11161 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    11221 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    11281 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    11341 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11401 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11461 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11521 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11581 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11641 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11701 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11761 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11821 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11881 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11941 tgccacctgg gtc
//
