LOCUS       sequence-411174-       12008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial double
            HA tag in domain II and a mutation in the P loop of Domain IV which 
            reduces trafficking to the cell surface.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 12008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: Cav2.2 HA EIVA (E1658A) pCAGGS
FEATURES             Location/Qualifiers
     source          1..12008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1704..1723
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3490..3516
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3520..3546
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9026..9045)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9091..9146
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9145..9164)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9507..9523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9507..9523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9520..9542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9531..9547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9555..9561,9562..9579,9580..9585))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9600..9621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9680..9875
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9726..9861
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9881..10015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9918..9937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9972..9991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10083..10100)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10254..10842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10334..10353)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11013..11804,11805..11873))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11636..11655
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11874..11978)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggsggg gggggggggg gsgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcststgm ymrscrksty yakrscyyyk bywamcmwkn ttcatgcctt
     1681 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     1741 caaagaattc ctcgagggta ccgggccccc cctcgaggtc gaagcttgat catggtccgc
     1801 ttcggggacg agctgggcgg ccgctatggg ggcgcgggcg gcgcggagcg ggcccggggc
     1861 ggcggggccg gcggggctgg cggcccgggc cccggggggc tgccgccggg ccagcgggtc
     1921 ctctacaaac agtcgatcgc gcagcgcgcg cggaccatgg cgctgtacaa ccccatcccg
     1981 gtcaagcaga actgcttcac cgtcaaccgc tcgctcttcg tcttcagcga ggacaacgtc
     2041 gtccgcaaat acgccaagcg catcaccgag tggcctccgt tcgagtacat gatcctggcc
     2101 accatcatcg ccaactgcat cgtgctggcc ctggagcagc acctccctga cggggacaag
     2161 acgcctatgt ccgagcgcct ggatgacacc gagccctact tcattgggat cttctgcttc
     2221 gaggcgggga tcaagatcct ggccctgggc tttgttctgc acaagggctc ctatctgcgg
     2281 aacggctgga acgtcatgga cttcgtggta gtcctcacgg ggattcttgc cacagccgga
     2341 accgacttcg acctgcgaac gctgagggct gtgcgtgtgc taaggcccct gaagctggtg
     2401 tctgggattc cgagcttgca agtggtgctc aagtccatca tgaaggccat ggtcccgctg
     2461 ctgcagatcg ggctgctgct cttcttcgcc atcctcatgt ttgccatcat cggcctcgag
     2521 ttctacatgg gcaagttcca caaggcctgc ttccccaaca gcacagaccc agatcccgtg
     2581 ggtgacttcc cctgtggcaa ggaggccccg gcccggctct gtgagggaga cacggagtgc
     2641 cgggagtact gggccggacc caactttggt atcaccaact ttgacaacat cctgtttgcc
     2701 atcttgaccg tgttccagtg catcaccatg gagggctgga ctgacatcct ctacaataca
     2761 aacgatgcag ccggcaacac gtggaactgg ctctacttca tccctctcat catcatcggc
     2821 tccttcttca tgctcaacct ggtgctgggt gtgctctcag gagagtttgc taaggagcgc
     2881 gagagagtgg agaaccgccg cgccttcctg aagctccggc ggcagcagca gattgagcgg
     2941 gagctcaacg ggtacctgga gtggatcttc aaggctgagg aagtcatgct ggctgaggag
     3001 gacaggaacg cagaggagaa gtcccctctg gatgcagtgt tgaagagagc agctgccaag
     3061 aagagcagaa gcgacctgat ccaggccgag gagggggagg gccggctcac aggcctctgt
     3121 gctcccggtt ccccgttcgc ccgtgccagc ctgaaaagcg ggaagacgga gagctcgtcg
     3181 tacttccgga ggaaggagaa gatgttccgc ttcttcatcc ggcgcatggt gaaggcacag
     3241 agcttctact ggacggtgct gtgtgtggtg gcactgaaca cactgtgcgt ggccatggtg
     3301 cactacaacc agccgcagcg gctcaccacg gcattgtact ttgcagagtt tgttttcctg
     3361 ggtctcttcc tcacagagat gtccctgaag atgtatggcc tggggcccag gagctacttc
     3421 cggtcctcct tcaactgttt tgactttggg gtcatcgtgg gcagtatctt tgaagtggtc
     3481 tgggctgctt acccctacga cgtccccgac tacgccggct acccgtacga cgtcccggac
     3541 tacgccggcg tcgacgtcaa gccagggacc tccttcggga tcagtgttct gcgggctctg
     3601 cgcttactga gaatcttcaa ggtcaccaaa tactggaact ccctacggaa cctggtggtg
     3661 tctctgctca actccatgaa gtccatcatc agcctcctct tcctgctctt cctgttcatt
     3721 gttgtctttg ctctgctggg gatgcagctg tttggaggcc agttcaactt taaagacgag
     3781 accccaacga ccaactttga caccttccct gctgctatcc tcaccgtctt ccagatcctg
     3841 accggagagg actggaatgc ggtcatgtac cacgggatcg agtcgcaagg tggcgtcagc
     3901 agaggaatgt tctcctcctt ctacttcatt gtcctcactc tgtttgggaa ctacaccctg
     3961 ctgaatgtct tcctggccat tgctgtggac aaccttgcca atgctcagga gctgaccaag
     4021 gatgaagagg agatggaaga agcagccaat cagaaactcg ccttgcagaa ggccaaggaa
     4081 gtggctgaag tcagccccat gtctgctgcg aacatctcca ttgccgccag gcagcagaac
     4141 tcggccaaag cgcgctcggt gtgggagcag cgagccagcc agctgcggct gcagaacctg
     4201 agggccagct gcgaggcgct gtacagcgag atggaccccg aggagaggct gcgctacgcc
     4261 accgcgcgcc acctgcggcc cgacgtgaag acgcacctgg accggccgct ggtggttgag
     4321 cccggccgcg acgctccgcg gggccccccg ggggggaagt cccggccgga tggctcggag
     4381 gcccccgagg gtgcggatcc cccgcgcagg catcaccggc accgagacaa ggacaaggct
     4441 cccgccacgg tcccctcggc gggggaacag gacagagccg aagctctgag ggcagagggc
     4501 ggggagctgg ggccccgcga ggagcggggg cggccacgcc gcagccgcag caaggaggcc
     4561 ccgggcgccc cggaggtgcg cagcgatcgc ggccgtggcc cgtgtcctga aggtggtcgg
     4621 cgccaccacc ggcgcgggtc accggaggag gcagctgaga gggagccccg ccgccaccgc
     4681 gcgcaccggc acgggcccga cccgggcaag gagggcccag cgtccgggac caggggcgag
     4741 cggcgcgcac gacaccgcac gggcccccgg gcgtgccccc gggaggctga gagcagcgag
     4801 gagcctgcgc ggcggcaccg ggctcggcac aaggcgccgc ccacccagga gaccgcggag
     4861 aaggacaagg aggctgccga gaaggggggc gaggccacgg aggccgagaa ggacaaggag
     4921 gctcggaacc accagcccaa ggagctgccc tgtgacctgg aggccatcgg catgctggga
     4981 gtgggcgctg tgcatacgct gcccagcacc tgtctgcaga aggtggagga gcagccagag
     5041 gatgcggaca accagcggaa cgtcacccgc atgggtagtc agcccccgga caccagcacc
     5101 actgtgcaca ttcctgtgac gctgaccggc ccccccgggg agaccacggt agtgcccagt
     5161 ggaaacgtgg acctggaaag tcaagcagag gggaagaagg aggtggaaac cagcgatgtg
     5221 atgaggagcg gccctcggcc cattgtcccg tacagttcca tgttctgttt aagcccaacc
     5281 aacctgctgc gccgctgctg ccactacatt gtgaccatgc gatacttcga gatggtcatc
     5341 ctcgtggtca tcgccctgag cagcatcgcc ctggctgccg aggaccccgt gagaacagac
     5401 tctcccagga ataatgctct gaagtacatg gactacatct tcacaggcgt cttcaccttc
     5461 gagatggtga taaagatgat cgatttggga ctgctgctac accctggagc ctatttccgg
     5521 gatctgtgga acattctgga cttcattgtg gtcagtgggg ccctggtggc atttgctttc
     5581 tcaggatcca aagggaaaga catcagcacc atcaaatccc tgagagtcct gcgtgtgctg
     5641 cggccactca agaccatcaa gcggctgccc aagctcaagg ccgtgtttga ctgtgtggtg
     5701 aactcactga aaaacgtcct caacatcctg atcgtctaca tgctcttcat gttcatcttt
     5761 gccgtcattg ccgtgcagct cttcaaaggg aagttcttct actgcacgga cgaatccaag
     5821 gagctggagc gtgactgcag gggtcagtat ttggattatg agaaggagga agtggaagct
     5881 caaccgaggc agtggaagaa atatgacttc cactatgaca acgtgctctg ggctctgcta
     5941 acgctcttca cggtgtccac aggggaaggg tggcccatgg tgctgaaaca ctctgtggat
     6001 gccacctacg aggagcaggg cccgagcccc gggtaccgca tggagctctc catcttctac
     6061 gtggtctact ttgtggtctt tcccttcttc tttgtcaaca tcttcgtggc cttgatcatc
     6121 atcaccttcc aggagcaggg ggacaaggtg atgtccgagt gcagcctgga gaagaacgag
     6181 agggcttgca tcgactttgc catcagcgcc agacccctga cgcggtacat gccccagaac
     6241 aagcagtcgt tccagtataa gacgtggacg ttcgtggtgt ccccaccttt tgagtacttc
     6301 atcatggcta tgatcgccct caacactgtg gtgctgatga tgaagttcta cgatgcgccg
     6361 tacgagtatg agctaatgct gaaatgcctg aacatcgtgt tcacgtccat gttctccatg
     6421 gagtgtgtgc tgaagatcat cgccttcggg gtgctgaact atttcagaga tgcctggaat
     6481 gtctttgact ttgtcacggt gttgggaagt attactgata ttttggtaac agagattgcg
     6541 aacaacttca tcaacctcag cttcctgcgc ctgttccggg ccgcgcggct catcaagctg
     6601 ctgcgccagg gctacaccat ccgcatcctg ctttggacct tcgtgcagtc cttcaaggcg
     6661 ctgccctacg tgtgtctcct catcgccatg ctcttcttca tctacgccat cattggcatg
     6721 caggtttttg ggaacatcgc cctggacgat gacaccagca tcaacaggca caataacttc
     6781 cggacgtttc tgcaggcctt gatgttgctg ttcaggagcg ccacgggggc ggcctggcac
     6841 gagataatgc tgtcgtgcct cagcagccgg gcctgcgacg agcactccaa tgccagcgag
     6901 tgcggcagcg actttgccta cttttacttc gtctccttca tcttcctctg ctcctttctg
     6961 atgttgaacc tctttgtggc tgtgatcatg gacaattttg agtaccttac acgggactct
     7021 tccatcttag ggcctcacca cctggatgag ttcatccggg tctgggctga atacgacccg
     7081 gccgcgtgtg ggcgcatcag ttacagtgac atgtttgaga tgctgaagca catgtcccca
     7141 cccctggggc tggggaagaa gtgccctgct cgagtcgcgt acaagcgcct ggtccgcatg
     7201 aacatgccca tctccagtga ggacatgacg gtgcacttca catccacgct gatggctctc
     7261 attcggacag cgctggacat caagctggca ccagctggga ccaagcagca ccagtgcgac
     7321 gcagagctga ggaaggagat ttcctgtgtg tgggccaatc tgccccagaa gaccctggac
     7381 ttactggtac caccccataa gcctgacgag atgacggtgg gcaaagtcta tgcagctctg
     7441 atgatcttcg acttctacaa acagaacaaa accagcagag accagactca gcaagccccc
     7501 ggaggcctgt cccagctggg ccccgtgtcc ctgttccacc cgctgaaggc cacgctggag
     7561 cagactcagc cggcgctgcg tggagcccgg gctttcctgc ggcagaagag ttctgcctcc
     7621 ctgagcaacg gcggggccgt acaaacccag gagagcggca tcaaggaatc ggtttcttgg
     7681 ggcactcaga ggacccagga tgtgctttgc gaggccaggg cacccctgga gcggggccac
     7741 tcagctgaga tcccagtcgg gcagcctggg acactggctg tagatgtcca gatgcagaac
     7801 atgactctga gtggccctga cgccgagccg cagccagggc tggagagcca gggccgagcc
     7861 gcctccatgc cccgcctggc cgcagaaaca cagcccgcgc ctgacgccag ccccatgaag
     7921 cgctccatct ccacgctggc cccgcgcccc cacacggccc ggctcggcag caccgcgctg
     7981 gaccgcccag cccccagcca ggccccgcac catcaccacc accgctgcca ccgccgcagg
     8041 gacaggaagc agcggtccct ggagaagggt cccagcctgt ctgctgacac agatggggcc
     8101 cccgacagca cggtggggcc aggactgccc acgggggagg gcccccccgg ctgcaggcgg
     8161 gagcgggagc gcaggcagga gcggggccgg tcccaggagc ggaggcagcc ctcgtcctcc
     8221 tcctcggaga agcatcgctt ctactcctgt gaccgcttcg ggggccgcga acccccacag
     8281 cccaagccct ccctcagtag tcaccccacg tcgccgacgg ccgggcagga gccgggaccc
     8341 cacccgcagg ggagtggttc ggttcacggg agccccctgc tgtccacatc tggtgctagc
     8401 accccgggcc gggggcggag gcagctcccc cagaccccct tgaccccccg ccccagcgtc
     8461 acctacaaga cggccaactc ctcgcctgtc cacttcgccg gggcaccgag cggcctcccc
     8521 gccttctccc ccggccggct cagccggggc ctctcagaac acaacgccct gctgcagagg
     8581 gacccgctca gccggcccct ggcgcccggc tcgcgcatcg gctccgaccc ctacctgggg
     8641 cagcggctgg acagcgaggc gcccgcccgt gccctgcccg aggacgcgcc cgcctttgag
     8701 gagaccgcgg ccagcaactc gggccgctcc tcccggacct cgtacgtgtc ctcactgacc
     8761 tcgcagcccc cgccgctgcg ccgcgtgccc aacggctacc actgcactct ggggctgggc
     8821 gggggcggcc gggcgcggcg cggctgccac caccccgacc gcgaccgccg gtgctagact
     8881 agttctagca agcttcgacg aattcgcatg cggccgctag ctcgaggtac cgatatcgag
     8941 ctcaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
     9001 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
     9061 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     9121 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     9181 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     9241 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     9301 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     9361 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     9421 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc
     9481 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     9541 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     9601 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9661 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     9721 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9781 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     9841 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     9901 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9961 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
    10021 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    10081 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    10141 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    10201 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10261 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10321 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10381 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10441 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10501 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10561 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10621 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10681 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10741 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10801 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    11041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    11101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    11161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    11221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    11281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    11341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    11401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    11461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    11521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    11581 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11641 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    11701 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11761 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11821 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11881 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11941 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    12001 cctgggtc
//
