LOCUS       sequence-411181-        4975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Consensus OR4 engineered from human OR4 family members.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ikegami K, de March CA, Nagai MH, Ghosh S, Do M, Sharma R, Bruguera 
            ES, Lu YE, Fukutani Y, Vaidehi N, Yohda M, Matsunami H
  TITLE     Structural instability and divergence from conserved residues 
            underlie intracellular retention of mammalian odorant receptors.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2957-2967. doi: 
            10.1073/pnas.1915520117. Epub 2020 Jan 23.
  PUBMED    31974307
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915520117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-02-11- 11"; volume: "117"; issue: "6"; pages: 
            "2957-2967"
COMMENT     Sequence Label: OR4-consensus
FEATURES             Location/Qualifiers
     source          1..4975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        121..500
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        501..704
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     655..675
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     701..725
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          840..972
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     955..974
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1017..1035
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2072..2193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2083..2102)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2137..2156
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2374..2829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2461..2480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2671..2692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2896..2915)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3015..3037
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3075..3093)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3161..3265
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3266..3334,3335..4126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3484..3503)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4297..4885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4786..4805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccatattatt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
       61 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
      121 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      181 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      241 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      301 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      361 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      421 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      481 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      541 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      601 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      661 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      721 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      781 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      841 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
      901 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
      961 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat
     1021 acgactcact ataggctagc atgaatggca cagaaggccc taacttctac gtgcccttct
     1081 ccaatgcgac gggtgtggta cgcgaattca cgcgtatgat ggagaatcat agtaacgtga
     1141 cagagttcat tctgttgggt ctcacccagt cacctgagct gcagctcttc cttttcgtcg
     1201 tcttcctcct tttttacgta gcgactgttt tggggaactt gctcatcgta gtcaccgtga
     1261 catcagaccc ccatctccat tctcccatgt attttttgct ggcgaatctt agctttatag
     1321 atatctgtta ttctagtgta actgcaccga aaatgatagt ggatttgctc tccgaaagga
     1381 aaacaatatc atttaatggt tgcatgacac agatattttt cctccacttc tttgggggtg
     1441 cagagatgtt cctccttgta gttatggctt atgatagata cgtggctatc tgtaaaccac
     1501 tgcattatct gactataatg aaccgccggg tttgtgtact tcttgttgct gttgcctggg
     1561 taggaggttt ccttcattca atcatccaac tggccttcat cgtacaactg ccattttgtg
     1621 gtccaaatgt aatagatagt ttcttttgtg acttgccgcc tgttttgaaa ttggcttgta
     1681 ccgatacgta tgtcctcggg ttgcttgtaa ttgcaaactc tggactcatt agtttgctgt
     1741 gctttcttct tcttctcatt tcttatgtcg tcattctcgt atctctgcgc agccattcca
     1801 gcgagggccg gagtaaggca ctctccacct gcactagtca cataactgtg gtgatactgt
     1861 tcttcggccc atgtatcttc atctataccc gccctttcac aacctttcct atggataaat
     1921 ttgtggctgt cttctatact gtcatcacgc ccctcctcaa cccccttatc tacacgctta
     1981 gaaacaagga gatgaaagcc gcaatgcgaa agctttggtc caggaaggta atatctagcc
     2041 ggaaataagc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     2101 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     2161 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     2221 tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     2281 ggtaaaatcg ataaggatcc gggctggcgt aatagcgaag aggcccgcac cgatcgccct
     2341 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
     2401 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2461 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2521 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2581 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     2641 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     2701 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     2761 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     2821 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     2881 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     2941 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     3001 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     3061 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     3121 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3181 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3241 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3301 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3361 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3421 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3481 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3541 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3601 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3661 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3721 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3781 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3841 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3901 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3961 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4021 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4081 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4141 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4201 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4441 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4681 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4921 cttttgctgg ccttttgctc acatggctcg acagatcttc aatattggcc attag
//
