LOCUS       sequence-411183-        4975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Consensus OR10 engineered from human OR10 family members.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ikegami K, de March CA, Nagai MH, Ghosh S, Do M, Sharma R, Bruguera 
            ES, Lu YE, Fukutani Y, Vaidehi N, Yohda M, Matsunami H
  TITLE     Structural instability and divergence from conserved residues 
            underlie intracellular retention of mammalian odorant receptors.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2957-2967. doi: 
            10.1073/pnas.1915520117. Epub 2020 Jan 23.
  PUBMED    31974307
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915520117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-02-11- 11"; volume: "117"; issue: "6"; pages: 
            "2957-2967"
COMMENT     Sequence Label: OR10-consensus
FEATURES             Location/Qualifiers
     source          1..4975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        118..497
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        498..701
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     652..672
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     698..722
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          837..969
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     952..971
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1014..1032
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2032..2043)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2069..2190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2080..2099)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2134..2153
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2371..2826
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2458..2477)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2668..2689
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2893..2912)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3012..3034
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3072..3090)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3158..3262
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3263..3331,3332..4123)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3481..3500)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4294..4882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4783..4802
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tattattcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
       61 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttggc
      121 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      181 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      241 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      301 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      361 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      421 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      481 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      541 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      601 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      661 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      721 tcactagaag ctttattgcg gtagtttatc acagttaaat tgctaacgca gtcagtgctt
      781 ctgacacaac agtctcgaac ttaagctgca gaagttggtc gtgaggcact gggcaggtaa
      841 gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga
      901 gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc
      961 tctccacagg tgtccactcc cagttcaatt acagctctta aggctagagt acttaatacg
     1021 actcactata ggctagcatg aatggcacag aaggccctaa cttctacgtg cccttctcca
     1081 atgcgacggg tgtggtacgc gaattcacgc gtatgcgaga gaatcaaacg ctcgtcaccg
     1141 agtttatatt gctggggttc tcctccctcc ctgaattgca agctctcttg tttctgctct
     1201 ttctcctgat ctacctgttg acgttgctgg ggaatctgct cataatactt gtcatctggg
     1261 tcgattcaca tctccatacc cccatgtact tttttctgtg caacctttct ttcagcgaga
     1321 tatgctatac cacggtcatt gtgccaaaga tgctggcgga tcttctctca cctgatcgaa
     1381 cgattagctt cctggggtgt gccacacaga tgttcttctt tttcttcttt gggagcacgg
     1441 agtgcttcct gcttacggta atggcctatg acagatatgt ggctatttgc catccgctgc
     1501 gatacccggt tatcatgagt cccaggcttt gtgcccagct tgtagcaggt agctgggctt
     1561 caggcttcct cgtatccctt gtgcaaacga gcctgatttt tcacttgccc ttttgcgggc
     1621 cgaatgagat caaccacttt ttttgcgata taccccctgt tctcaaactg gcttgtgcgg
     1681 acactacagt caatgagatt gtcatattcg ttgtgacgat tctcgttctg cttggaccgt
     1741 tcctgcttat actgctgagt tacgtcttta ttgtagccgc tattctcaag ataccatcag
     1801 cagagggtcg aagaaaagcc tttagcacgt gcgctagcca tctcaccgta gttatcttgc
     1861 attacggctg cgcgtcattt atatacctga gacctaagtc taactacagc cccgaagaca
     1921 agctggtatc agtgacctat acagtactca caccgctcct gaaccccatt atatactctc
     1981 ttaggaataa agaagtgaag aatgcactga agcgccttct tggaaggaag ctcgcccttc
     2041 aataagcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     2101 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     2161 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     2221 caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     2281 aaaatcgata aggatccggg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     2341 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     2401 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     2461 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2521 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2581 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     2641 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     2701 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     2761 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     2821 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     2881 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     2941 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3001 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3061 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3121 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3181 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3241 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3301 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3361 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3421 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3481 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     3541 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3601 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3661 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3721 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3781 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3841 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3901 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3961 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4021 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4081 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4141 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4201 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4261 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4321 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4381 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4441 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4501 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4561 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4621 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4681 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4741 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4801 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4861 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4921 ttgctggcct tttgctcaca tggctcgaca gatcttcaat attggccatt agcca
//
