LOCUS       sequence-411184-        4975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Consensus OR2 engineered from human OR2 family members.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ikegami K, de March CA, Nagai MH, Ghosh S, Do M, Sharma R, Bruguera 
            ES, Lu YE, Fukutani Y, Vaidehi N, Yohda M, Matsunami H
  TITLE     Structural instability and divergence from conserved residues 
            underlie intracellular retention of mammalian odorant receptors.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2957-2967. doi: 
            10.1073/pnas.1915520117. Epub 2020 Jan 23.
  PUBMED    31974307
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915520117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-02-11- 11"; volume: "117"; issue: "6"; pages: 
            "2957-2967"
COMMENT     Sequence Label: OR2-consensus
FEATURES             Location/Qualifiers
     source          1..4975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        116..495
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        496..699
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     650..670
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     696..720
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          835..967
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     950..969
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1012..1030
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2067..2188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2078..2097)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2132..2151
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2369..2824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2456..2475)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2666..2687
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2891..2910)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3010..3032
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3070..3088)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3156..3260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3261..3329,3330..4121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3479..3498)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4292..4880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4781..4800
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
       61 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttggcat
      121 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      181 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      241 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      301 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      361 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      421 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
      481 atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt
      541 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      601 caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc
      661 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
      721 actagaagct ttattgcggt agtttatcac agttaaattg ctaacgcagt cagtgcttct
      781 gacacaacag tctcgaactt aagctgcaga agttggtcgt gaggcactgg gcaggtaagt
      841 atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga
      901 agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc
      961 tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac ttaatacgac
     1021 tcactatagg ctagcatgaa tggcacagaa ggccctaact tctacgtgcc cttctccaat
     1081 gcgacgggtg tggtacgcga attcacgcgt atggaagaaa atcaaacaag tagcactgac
     1141 tttattctcc tcggcttgtt tgaccatccc cggcttgaac tcctcctgtt tgtcctcata
     1201 ctcctcatat accttttggc cctccttggc aacgggctca tcattctgct tatccacctg
     1261 gattcaaggc ttcacacgcc catgtacttc ttcctctcac aacttagctt gatggatttg
     1321 tgctacactt ccacgaccgt tcctaaaatg ctggtaaacc tgctgtctgg cgacaagacg
     1381 attagtttcg ctggttgcgg tgcacagctc ttcctttatc tcactttggg cggcaccgag
     1441 tgcttgctcc ttgccgttat ggcatatgac agatatgtag caatctgtca ccccttgcgc
     1501 taccccgtat tgatgaaccc tagagtttgc ctcctgcttg ccgcgggttc ttggctgctg
     1561 ggatcccttg actcccttat ccacacagtg ttgacgctct ctctgccctt ttgtggcagt
     1621 agggaaatca accatttctt ttgtgaagtg cccgcgctgc tcaagcttgc ttgtgctgat
     1681 acatccttga acgaaactgt tatgttcgta tgctgtgtgc tcatgctcct catacctctg
     1741 tctctcatcc tcgtaagtta tggcagaatc ttgcttgctg tcttgcgaat gcaatccgcc
     1801 gagggtcgca gaaaagcttt ttcaacatgt tcttctcatc tgacagtggt gggattgttc
     1861 tatggagctg cgatctacat gtatttgcag cctaaatcct accattctcc agagcaggat
     1921 aaggtggtaa gcttgttcta caccatattg acgccgatgc tcaacccctt gatatactca
     1981 cttcgcaata aggaagttaa gggggctctg cggcgagtgc ttggtaaatg taggagcctt
     2041 taagcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     2101 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2161 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2221 ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     2281 aatcgataag gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     2341 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     2401 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     2461 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     2521 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     2581 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     2641 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     2701 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2761 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     2821 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     2881 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     2941 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3001 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3061 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3121 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3181 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3241 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3301 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3361 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3421 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3481 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3541 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3601 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3661 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3721 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3781 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     3841 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3901 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3961 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4021 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4081 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4141 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4201 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4261 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4321 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4381 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4441 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4501 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4561 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4621 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4681 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4741 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4801 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4861 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4921 gctggccttt tgctcacatg gctcgacaga tcttcaatat tggccattag ccata
//
