LOCUS       sequence-411185-        4975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Consensus OR1 engineered from human OR1 family members.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ikegami K, de March CA, Nagai MH, Ghosh S, Do M, Sharma R, Bruguera 
            ES, Lu YE, Fukutani Y, Vaidehi N, Yohda M, Matsunami H
  TITLE     Structural instability and divergence from conserved residues 
            underlie intracellular retention of mammalian odorant receptors.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2957-2967. doi: 
            10.1073/pnas.1915520117. Epub 2020 Jan 23.
  PUBMED    31974307
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915520117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-02-11- 11"; volume: "117"; issue: "6"; pages: 
            "2957-2967"
COMMENT     Sequence Label: OR1-consensus
FEATURES             Location/Qualifiers
     source          1..4975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        108..487
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        488..691
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     642..662
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     688..712
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          827..959
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     942..961
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1004..1022
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2059..2180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2070..2089)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2124..2143
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2361..2816
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2448..2467)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2658..2679
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2883..2902)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3002..3024
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3062..3080)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3148..3252
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3253..3321,3322..4113)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3471..3490)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4284..4872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4773..4792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
       61 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttggc attgattatt
      121 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      181 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      241 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      301 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      361 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      421 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      481 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
      541 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      601 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
      661 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcactagaag
      721 ctttattgcg gtagtttatc acagttaaat tgctaacgca gtcagtgctt ctgacacaac
      781 agtctcgaac ttaagctgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt
      841 tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt
      901 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg
      961 tgtccactcc cagttcaatt acagctctta aggctagagt acttaatacg actcactata
     1021 ggctagcatg aatggcacag aaggccctaa cttctacgtg cccttctcca atgcgacggg
     1081 tgtggtacgc gaattcacgc gtatggaagg agagaaccag tctagtgtat ctgagttcct
     1141 tctccttgga ctgtcctctc agccggagca acagaacctt ctgttcgcac tgttcctgtc
     1201 aatgtacctc gttaccgtcc tcggcaatct cctgataata cttgctattt cttccgattc
     1261 acatttgcac acacccatgt actttttcct gagtaacctg tcctttaccg acatttgttt
     1321 tagcagtgtc actgtcccca aaatgcttgt aaacatacaa acacagtcaa agagtattag
     1381 ctatgcaggt tgtttgacgc aaatgtactt cttcatagct tttgggaatc tggacagttt
     1441 tctcttggcg gtcatggcgt acgataggta cgtagcaatc tgtcatcctc ttcattacac
     1501 aacaatcatg tcaccgaggt tgtgcgttct cctcgtagcg ttgtcatggg ttttgtctaa
     1561 tttgcacgcc ctgctccaca ctttgcttat ggcgcggctc tctttttgcg cctccaatga
     1621 aatcccccac ttcttttgtg atttgagccc acttctcaag ctctcatgca gcgacacaca
     1681 cgttaacgag ctcgttatat ttacagaagg actgctcgta atagtcactc cgttcctctg
     1741 tatactggtc agctacgtca ggatagtatc caccgttctt aagatccctt cagcgaaggg
     1801 taagtggaaa gcattttcca cttgcggtag ccacctgacc gttgtttctt tgttctacgg
     1861 cactattatt ggggtctact ttcaaccgct gagtacctat tccgtcaagg atactgtcgc
     1921 cacggtgatg tatacggtag tcaccccgat gttgaaccca ttcatttata gtcttaggaa
     1981 caaagatatg aaaggcgctt tgcgaaagct gcttagtcgc agaaagtctt cttaagcggc
     2041 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     2101 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     2161 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     2221 gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
     2281 aggatccggg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2341 gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2401 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2461 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2521 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     2581 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2641 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     2701 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     2761 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg
     2821 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     2881 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     2941 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3001 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3061 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3121 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3181 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3241 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3301 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3361 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3421 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3481 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3541 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3601 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3661 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3721 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3781 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3841 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3901 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3961 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4021 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4081 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4141 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4201 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4261 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4321 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4381 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4441 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4501 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4561 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4621 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4681 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4741 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4801 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4861 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4921 tttgctcaca tggctcgaca gatcttcaat attggccatt agccatatta ttcat
//
