LOCUS       sequence-411187-        4978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Consensus OR8 engineered from human OR8 family members.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ikegami K, de March CA, Nagai MH, Ghosh S, Do M, Sharma R, Bruguera 
            ES, Lu YE, Fukutani Y, Vaidehi N, Yohda M, Matsunami H
  TITLE     Structural instability and divergence from conserved residues 
            underlie intracellular retention of mammalian odorant receptors.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2957-2967. doi: 
            10.1073/pnas.1915520117. Epub 2020 Jan 23.
  PUBMED    31974307
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915520117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-02-11- 11"; volume: "117"; issue: "6"; pages: 
            "2957-2967"
COMMENT     Sequence Label: OR8-consensus
FEATURES             Location/Qualifiers
     source          1..4978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        109..488
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        489..692
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     643..663
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     689..713
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          828..960
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     943..962
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1005..1023
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2063..2184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2074..2093)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2128..2147
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2365..2820
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2452..2471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2662..2683
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2887..2906)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3006..3028
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3066..3084)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3152..3256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3257..3325,3326..4117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3475..3494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4288..4876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4777..4796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
       61 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttgg cattgattat
      121 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      181 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      241 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      301 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      361 tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      421 agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      481 ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac
      541 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      601 aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc
      661 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa
      721 gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa
      781 cagtctcgaa cttaagctgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg
      841 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
      901 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
      961 gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat
     1021 aggctagcat gaatggcaca gaaggcccta acttctacgt gcccttctcc aatgcgacgg
     1081 gtgtggtacg cgaattcacg cgtatggccg caagaaacca cacgactgtg acggagttca
     1141 tactcacagg actcaccgac cacccagagt tgcaaatgcc actctttctt ctctttctcg
     1201 gcatatatct tgtcacagtc gtcgggaact tggggatgat tacgctgatc cgattggatt
     1261 ctcaattgca cacaccaatg tactttttcc ttagtaacct tagctttatt gatctttgct
     1321 atagttcagt tattaccccc aaaatgctcg tcaattttct tagtaagaag aatatcatat
     1381 ctttcgttgg atgcatgaca caactctttt tctttctttt cttcgttatc agcgaatgct
     1441 atcttttgtc ttcaatggcc tacgatcgct atgtggccat ctgtaatccc ctgctgtata
     1501 tggtgattat gagtccaaga gtctgcctcc tcctcgtggc gggcccatac gtctatgggt
     1561 ttcttggcgc actcgtccat acggggtgta tgtttcgact ttcattttgc gattctaaca
     1621 taataaatca tttcttctgc gacatccttc cccttttggc tctgtcatgc agtgacactt
     1681 atatcaatga acttgttatt ttcatcattg ctggtataaa ccttcttgta tccctgctca
     1741 cagtgttggt ttcttatgcc tttatacttt cttctatcct taggattaac agcacggaag
     1801 gtcgctccaa agcattcagc acctgttcca gtcatttgct ggctgtaact atattctacg
     1861 gaagtttgat cttcatgtat ctgaagcctt ctagttccca ctctttggat caggacaagg
     1921 tcgccagcgt tttctatact atcgtcattc cgatgcttaa tccactgatc tactctcttc
     1981 gcaataaaga cgttaaaaac gctttgaaaa agacacttat ccgaaggcaa ttcagttaag
     2041 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     2101 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     2161 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     2221 cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     2281 gataaggatc cgggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     2341 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     2401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2521 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2581 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2701 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2761 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     2821 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     2881 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     2941 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     3001 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     3061 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     3121 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3481 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3781 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4921 gccttttgct cacatggctc gacagatctt caatattggc cattagccat attattca
//
