LOCUS       sequence-411189-        4981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Consensus OR6 engineered from human OR6 family members.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ikegami K, de March CA, Nagai MH, Ghosh S, Do M, Sharma R, Bruguera 
            ES, Lu YE, Fukutani Y, Vaidehi N, Yohda M, Matsunami H
  TITLE     Structural instability and divergence from conserved residues 
            underlie intracellular retention of mammalian odorant receptors.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2957-2967. doi: 
            10.1073/pnas.1915520117. Epub 2020 Jan 23.
  PUBMED    31974307
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915520117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-02-11- 11"; volume: "117"; issue: "6"; pages: 
            "2957-2967"
COMMENT     Sequence Label: OR6-consensus
FEATURES             Location/Qualifiers
     source          1..4981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        111..490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        491..694
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     645..665
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     691..715
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          830..962
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     945..964
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1007..1025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2068..2189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2079..2098)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2133..2152
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2370..2825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2457..2476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2667..2688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2892..2911)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3011..3033
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3071..3089)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3157..3261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3262..3330,3331..4122)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3480..3499)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4293..4881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4782..4801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata
       61 tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt ggcattgatt
      121 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      181 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      241 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      301 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      361 tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      421 ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      481 tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc
      541 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      601 tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag
      661 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcactag
      721 aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg cttctgacac
      781 aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      841 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      901 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
      961 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1021 ataggctagc atgaatggca cagaaggccc taacttctac gtgcccttct ccaatgcgac
     1081 gggtgtggta cgcgaattca cgcgtatggg taaccataca cgggtcaccg aattcatact
     1141 gcttggtttt cccgacgacc ctgaattgca agtcctgctc ttccttctcc tcttgttgac
     1201 ctatcttctg accttgacgg gaaaccttct gataattact ctggtgctcc ttgactctca
     1261 ccttcataca ccaatgtact tcttcttgcg aaatctgagt tttctggaga tatggtacac
     1321 aaccgtgact attccaaaaa tgctcgctaa tattcttacc ggagacaaga caatctcatt
     1381 caatggctgc atgacacaat tgtatttctt tatattcctg ggctgcactg agtttttcct
     1441 ccttgcggca atgtcctatg atcgctacgt tgcgatttgt aagccacttc attatcccac
     1501 gatcatgtcc tcccgcctct gtacccagtt ggtcctcgga tcctgggtag caggatttct
     1561 cataatcttg cctcccctca ttcttatttc tcaactcccg ttctgcggct ctaacgtcat
     1621 aaaccatttt ttttgcgata tctctccact tctccagctt gcatgtaccg acacctccct
     1681 ccttgaattg gttgattttc tcctggccgt ggtgaccctg cttgtcacct tgcttcttgt
     1741 tatcctttcc tatacctata ttatcaggac catcttgcga ataccgtccg ctcaaggtcg
     1801 gaagaaggct ttctcaacct gcgcgtccca tttgaccgtg gtatccatct tttatggaag
     1861 ctgcatcttt atgtacgtta gacccagcgc caaagaaagc gtcaacctga ataaaggtgt
     1921 ggctgtactg tacactgtgg tcaccccact tttgaaccca tttatctata cactcaggaa
     1981 taaagaggta aagcaggcgc ttaaaaagac cctcaaaaaa atcaaattcc tgtccaagaa
     2041 gtaagcggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     2101 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     2161 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     2221 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     2281 aaatcgataa ggatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     2341 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
     2401 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     2461 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     2521 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     2581 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     2641 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     2701 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     2761 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     2821 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     2881 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     2941 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3001 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3061 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     3121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3181 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3241 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3301 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3361 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3421 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3481 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     3541 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3601 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3661 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3721 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3781 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3841 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3901 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3961 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4021 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4081 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4141 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4201 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4261 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4321 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4381 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4441 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4501 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4561 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4621 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4681 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4741 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4801 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4861 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4921 tgctggcctt ttgctcacat ggctcgacag atcttcaata ttggccatta gccatattat
     4981 t
//
