LOCUS       sequence-411190-        4984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Consensus OR51 engineered from human OR51 family members.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ikegami K, de March CA, Nagai MH, Ghosh S, Do M, Sharma R, Bruguera 
            ES, Lu YE, Fukutani Y, Vaidehi N, Yohda M, Matsunami H
  TITLE     Structural instability and divergence from conserved residues 
            underlie intracellular retention of mammalian odorant receptors.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2957-2967. doi: 
            10.1073/pnas.1915520117. Epub 2020 Jan 23.
  PUBMED    31974307
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915520117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-02-11- 11"; volume: "117"; issue: "6"; pages: 
            "2957-2967"
COMMENT     Sequence Label: OR51-consensus
FEATURES             Location/Qualifiers
     source          1..4984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        113..492
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        493..696
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     647..667
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     693..717
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          832..964
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     947..966
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1009..1027
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2073..2194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2084..2103)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2138..2157
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2375..2830
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2462..2481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2672..2693
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2897..2916)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3016..3038
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3076..3094)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3162..3266
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3267..3335,3336..4127)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3485..3504)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4298..4886
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4787..4806
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
       61 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttggcattga
      121 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      181 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      241 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      301 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      361 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      421 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
      481 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
      541 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      601 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
      661 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcact
      721 agaagcttta ttgcggtagt ttatcacagt taaattgcta acgcagtcag tgcttctgac
      781 acaacagtct cgaacttaag ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc
      841 aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga
      901 ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc
      961 acaggtgtcc actcccagtt caattacagc tcttaaggct agagtactta atacgactca
     1021 ctataggcta gcatgaatgg cacagaaggc cctaacttct acgtgccctt ctccaatgcg
     1081 acgggtgtgg tacgcgaatt cacgcgtatg agcataaaca actcaacgtc atccgcggcg
     1141 acatttcttc tcacaggaat acccgggctt gaagcagcgc acatctggat cagcatccct
     1201 ttttgtttta tgtatttgat cgcgatattg ggcaatggaa ccatcctctt tatcattagg
     1261 actgagccgt cattgcacga acccatgtac tattttctgt ctatgttggc tgctacggat
     1321 ctgggtctca gcttgagcac ccttccgacg gtacttggga tattttggtt taatgcgagg
     1381 gaaatcagtt ttaatgcatg ctttgcacag atgttcttca tacacgggtt cagtttcatg
     1441 gagagcagcg tgctccttgc catggcgttc gacaggtttg ttgcgatatg caatcccctg
     1501 cgctacgcct ctatactcac aaatactaga gtggcgaaaa tcggccttgc gatactgacc
     1561 cggagcttct tgctcatact tccgcttccc tttctgttga agcgacttcc gtattgccac
     1621 tctaacgtat tgagtcactc ttactgcctg caccaggatg tgatgaaact ggcatgcgcc
     1681 gatattcgat tcaacagcat ctatggactg tttgtcgttc tgtctaccat gggattggat
     1741 agcttgctca tactgttctc ctatattctc atactgaaga ccgtcctggg aatcgctagt
     1801 agggaagaaa ggctgaaagc attgaacact tgcgttagcc acatctgtgc ggtgctcgta
     1861 ttttacgtcc ccatgatagg tctctcactc gttcatagat ttggtaagca tgtgccacct
     1921 gttgttcacg tactgatggc caacgtgtac ctcctcgtgc ctcccgttat gaatcccatt
     1981 atatacagcg tgaaaacaaa acagattcgg aagcgcattc ttagactgtt ctcactgaag
     2041 aaaatataag cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     2101 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2161 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2221 gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     2281 tggtaaaatc gataaggatc cgggctggcg taatagcgaa gaggcccgca ccgatcgccc
     2341 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     2401 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2461 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2521 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2581 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2641 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2701 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2761 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2821 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2881 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     2941 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     3001 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     3061 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     3121 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3181 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     3241 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     3301 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     3361 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     3421 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     3481 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     3541 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     3601 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     3661 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3721 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3781 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3841 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3901 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3961 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4021 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4081 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4141 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4201 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4261 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4321 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4381 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4441 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4501 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4561 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4621 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4681 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4741 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4801 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4861 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4921 ccttttgctg gccttttgct cacatggctc gacagatctt caatattggc cattagccat
     4981 atta
//
