LOCUS       sequence-411196-       12617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial 
            pHfluorin tag in domain II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12617)
  TITLE     Dolphin Lab Plasmids 2023
REFERENCE   2  (bases 1 to 12617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Cav2.2 pHfluorin pCAGGS
FEATURES             Location/Qualifiers
     source          1..12617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          110..1126
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1050..1072
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1134..1153
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2965..3675
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2992..3020)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3615..3635
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(9191..9210)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9256..9311
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9310..9329)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9672..9688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9672..9688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9685..9707)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9696..9712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9720..9726,9727..9744,9745..9750))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9765..9786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9845..10040
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9891..10026
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10046..10180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10083..10102)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10137..10156
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10248..10265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10419..11007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10499..10518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11178..11969,11970..12038))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11801..11820
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12039..12143)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        12174..12553
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
       61 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
      121 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      181 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
      241 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      301 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
      361 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
      421 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
      481 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
      541 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
      601 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
      661 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
      721 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
      781 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
      841 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
      901 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
      961 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     1021 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     1081 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1141 gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgagggta ccgggccccc
     1201 cctcgaggtc gaagcttgat catggtccgc ttcggggacg agctgggcgg ccgctatggg
     1261 ggcgcgggcg gcgcggagcg ggcccggggc ggcggggccg gcggggctgg cggcccgggc
     1321 cccggggggc tgccgccggg ccagcgggtc ctctacaaac agtcgatcgc gcagcgcgcg
     1381 cggaccatgg cgctgtacaa ccccatcccg gtcaagcaga actgcttcac cgtcaaccgc
     1441 tcgctcttcg tcttcagcga ggacaacgtc gtccgcaaat acgccaagcg catcaccgag
     1501 tggcctccgt tcgagtacat gatcctggcc accatcatcg ccaactgcat cgtgctggcc
     1561 ctggagcagc acctccctga cggggacaag acgcctatgt ccgagcgcct ggatgacacc
     1621 gagccctact tcattgggat cttctgcttc gaggcgggga tcaagatcct ggccctgggc
     1681 tttgttctgc acaagggctc ctatctgcgg aacggctgga acgtcatgga cttcgtggta
     1741 gtcctcacgg ggattcttgc cacagccgga accgacttcg acctgcgaac gctgagggct
     1801 gtgcgtgtgc taaggcccct gaagctggtg tctgggattc cgagcttgca ggtggtgctc
     1861 aagtccatca tgaaggccat ggtcccgctg ctgcagatcg ggctgctgct cttcttcgcc
     1921 atcctcatgt ttgccatcat cggcctcgag ttctacatgg gcaagttcca caaggcctgc
     1981 ttccccaaca gcacagaccc agatcccgtg ggtgacttcc cctgtggcaa ggaggccccg
     2041 gcccggctct gtgagggaga cacggagtgc cgggagtact gggccggacc caactttggt
     2101 atcaccaact ttgacaacat cctgtttgcc atcttgaccg tgttccagtg catcaccatg
     2161 gagggctgga ctgacatcct ctacaataca aacgatgcag ccggcaacac gtggaactgg
     2221 ctctacttca tccctctcat catcatcggc tccttcttca tgctcaacct ggtgctgggt
     2281 gtgctctcag gagagtttgc taaggagcgc gagagagtgg agaaccgccg cgccttcctg
     2341 aagctccggc ggcagcagca gattgagcgg gagctcaacg ggtacctgga gtggatcttc
     2401 aaggctgagg aagtcatgct ggctgaggag gacaggaacg cagaggagaa gtcccctctg
     2461 gatgcagtgt tgaagagagc agctgccaag aagagcagaa gcgacctgat ccaggccgag
     2521 gagggggagg gccggctcac aggcctctgt gctcccggtt ccccgttcgc ccgtgccagc
     2581 ctgaaaagcg ggaagacgga gagctcgtcg tacttccgga ggaaggagaa gatgttccgc
     2641 ttcttcatcc ggcgcatggt gaaggcacag agcttctact ggacggtgct gtgtgtggtg
     2701 gcactgaaca cactgtgcgt ggccatggtg cactacaacc agccgcagcg gctcaccacg
     2761 gcattgtact ttgcagagtt tgttttcctg ggtctcttcc tcacagagat gtccctgaag
     2821 atgtatggcc tggggcccag gagctacttc cggtcctcct tcaactgttt tgactttggg
     2881 gtcatcgtgg gcagtatctt tgaagtggtc tgggctgctg tcgactccgg tagcactagc
     2941 ggcggaagcg gcgggacagg tggaagtaaa ggagaagaac ttttcactgg agttgtccca
     3001 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
     3061 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     3121 cctgttcctt ggccaacact tgtcactact ttaacttatg gtgttcaatg cttttcaaga
     3181 tacccagatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     3241 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     3301 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     3361 ggaaacattc ttggacacaa attggaatac aactataacg atcaccaggt gtacatcatg
     3421 gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttagacacaa cattgaagat
     3481 ggaggcgttc aactagcaga ccattatcaa caaaatactc caattggcga tgggcccgtc
     3541 cttttaccag acaaccatta cctgtttaca acttctactc tttcgaaaga tcccaacgaa
     3601 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     3661 gatgaactat acaaaagcgg aggcacaggt ggcagcggcg gaaccggagg ctccgtcgac
     3721 gtcaagccag ggacctcctt cgggatcagt gttctgcggg ctctgcgctt actgagaatc
     3781 ttcaaggtca ccaaatactg gaactcccta cggaacctgg tggtgtctct gctcaactcc
     3841 atgaagtcca tcatcagcct cctcttcctg ctcttcctgt tcattgttgt ctttgctctg
     3901 ctggggatgc agctgtttgg aggccagttc aactttaaag acgagacccc aacgaccaac
     3961 tttgacacct tccctgctgc tatcctcacc gtcttccaga tcctgaccgg agaggactgg
     4021 aatgcggtca tgtaccacgg gatcgagtcg caaggtggcg tcagcagagg aatgttctcc
     4081 tccttctact tcattgtcct cactctgttt gggaactaca ccctgctgaa tgtcttcctg
     4141 gccattgctg tggacaacct tgccaatgct caggagctga ccaaggatga agaggagatg
     4201 gaagaagcag ccaatcagaa actcgccttg cagaaggcca aggaagtggc tgaagtcagc
     4261 cccatgtctg ctgcgaacat ctccattgcc gccaggcagc agaactcggc caaagcgcgc
     4321 tcggtgtggg agcagcgagc cagccagctg cggctgcaga acctgagggc cagctgcgag
     4381 gcgctgtaca gcgagatgga ccccgaggag aggctgcgct acgccaccgc gcgccacctg
     4441 cggcccgacg tgaagacgca cctggaccgg ccgctggtgg ttgagcccgg ccgcgacgct
     4501 ccgcggggcc ccccgggggg gaagtcccgg ccggatggct cggaggcccc cgagggtgcg
     4561 gatcccccgc gcaggcatca ccggcaccga gacaaggaca aggctcccgc cacggtcccc
     4621 tcggcggggg aacaggacag agccgaagct ctgagggcag agggcgggga gctggggccc
     4681 cgcgaggagc gggggcggcc acgccgcagc cgcagcaagg aggccccggg cgccccggag
     4741 gtgcgcagcg atcgcggccg tggcccgtgt cctgaaggtg gtcggcgcca ccaccggcgc
     4801 gggtcaccgg aggaggcagc tgagagggag ccccgccgcc accgcgcgca ccggcacggg
     4861 cccgacccgg gcaaggaggg cccagcgtcc gggaccaggg gcgagcggcg cgcacgacac
     4921 cgcacgggcc cccgggcgtg cccccgggag gctgagagca gcgaggagcc tgcgcggcgg
     4981 caccgggctc ggcacaaggc gccgcccacc caggagaccg cggagaagga caaggaggct
     5041 gccgagaagg ggggcgaggc cacggaggcc gagaaggaca aggaggctcg gaaccaccag
     5101 cccaaggagc tgccctgtga cctggaggcc atcggcatgc tgggagtggg cgctgtgcat
     5161 acgctgccca gcacctgtct gcagaaggtg gaggagcagc cagaggatgc ggacaaccag
     5221 cggaacgtca cccgcatggg tagtcagccc ccggacacca gcaccactgt gcacattcct
     5281 gtgacgctga ccggcccccc cggggagacc acggtagtgc ccagtggaaa cgtggacctg
     5341 gaaagtcaag cagaggggaa gaaggaggtg gaaaccagcg atgtgatgag gagcggccct
     5401 cggcccattg tcccgtacag ttccatgttc tgtttaagcc caaccaacct gctgcgccgc
     5461 tgctgccact acattgtgac catgcgatac ttcgagatgg tcatcctcgt ggtcatcgcc
     5521 ctgagcagca tcgccctggc tgccgaggac cccgtgagaa cagactctcc caggaataat
     5581 gctctgaagt acatggacta catcttcaca ggcgtcttca ccttcgagat ggtgataaag
     5641 atgatcgatt tgggactgct gctacaccct ggagcctatt tccgggatct gtggaacatt
     5701 ctggacttca ttgtggtcag tggggccctg gtggcatttg ctttctcagg atccaaaggg
     5761 aaagacatca gcaccatcaa atccctgaga gtcctgcgtg tgctgcggcc actcaagacc
     5821 atcaagcggc tgcccaagct caaggccgtg tttgactgtg tggtgaactc actgaaaaac
     5881 gtcctcaaca tcctgatcgt ctacatgctc ttcatgttca tctttgccgt cattgccgtg
     5941 cagctcttca aagggaagtt cttctactgc acggacgaat ccaaggagct ggagcgtgac
     6001 tgcaggggtc agtatttgga ttatgagaag gaggaagtgg aagctcaacc gaggcagtgg
     6061 aagaaatatg acttccacta tgacaacgtg ctctgggctc tgctaacgct cttcacggtg
     6121 tccacagggg aagggtggcc catggtgctg aaacactctg tggatgccac ctacgaggag
     6181 cagggcccga gccccgggta ccgcatggag ctctccatct tctacgtggt ctactttgtg
     6241 gtctttccct tcttctttgt caacatcttc gtggccttga tcatcatcac cttccaggag
     6301 cagggggaca aggtgatgtc cgagtgcagc ctggagaaga acgagagggc ttgcatcgac
     6361 tttgccatca gcgccagacc cctgacgcgg tacatgcccc agaacaagca gtcgttccag
     6421 tataagacgt ggacgttcgt ggtgtcccca ccttttgagt acttcatcat ggctatgatc
     6481 gccctcaaca ctgtggtgct gatgatgaag ttctacgatg cgccgtacga gtatgagcta
     6541 atgctgaaat gcctgaacat cgtgttcacg tccatgttct ccatggagtg tgtgctgaag
     6601 atcatcgcct tcggggtgct gaactatttc agagatgcct ggaatgtctt tgactttgtc
     6661 acggtgttgg gaagtattac tgatattttg gtaacagaga ttgcgaacaa cttcatcaac
     6721 ctcagcttcc tgcgcctgtt ccgggccgcg cggctcatca agctgctgcg ccagggctac
     6781 accatccgca tcctgctttg gaccttcgtg cagtccttca aggcgctgcc ctacgtgtgt
     6841 ctcctcatcg ccatgctctt cttcatctac gccatcattg gcatgcaggt ttttgggaac
     6901 atcgccctgg acgatgacac cagcatcaac aggcacaata acttccggac gtttctgcag
     6961 gccttgatgt tgctgttcag gagcgccacg ggggaggcct ggcacgagat aatgctgtcg
     7021 tgcctcagca gccgggcctg cgacgagcac tccaatgcca gcgagtgcgg cagcgacttt
     7081 gcctactttt acttcgtctc cttcatcttc ctctgctcct ttctgatgtt gaacctcttt
     7141 gtggctgtga tcatggacaa ttttgagtac cttacacggg actcttccat cttagggcct
     7201 caccacctgg atgagttcat ccgggtctgg gctgaatacg acccggccgc gtgtgggcgc
     7261 atcagttaca gtgacatgtt tgagatgctg aagcacatgt ccccacccct ggggctgggg
     7321 aagaagtgcc ctgctcgagt cgcgtacaag cgcctggtcc gcatgaacat gcccatctcc
     7381 agtgaggaca tgacggtgca cttcacatcc acgctgatgg ctctcattcg gacagcgctg
     7441 gacatcaagc tggcaccagc tgggaccaag cagcaccagt gcgacgcaga gctgaggaag
     7501 gagatttcct gtgtgtgggc caatctgccc cagaagaccc tggacttact ggtaccaccc
     7561 cataagcctg acgagatgac ggtgggcaaa gtctatgcag ctctgatgat cttcgacttc
     7621 tacaaacaga acaaaaccag cagagaccag actcagcaag cccccggagg cctgtcccag
     7681 ctgggccccg tgtccctgtt ccacccgctg aaggccacgc tggagcagac tcagccggcg
     7741 ctgcgtggag cccgggcttt cctgcggcag aagagttctg cctccctgag caacggcggg
     7801 gccgtacaaa cccaggagag cggcatcaag gaatcggttt cttggggcac tcagaggacc
     7861 caggatgtgc tttgcgaggc cagggcaccc ctggagcggg gccactcagc tgagatccca
     7921 gtcgggcagc ctgggacact ggctgtagat gtccagatgc agaacatgac tctgagtggc
     7981 cctgacgccg agccgcagcc agggctggag agccagggcc gagccgcctc catgccccgc
     8041 ctggccgcag aaacacagcc cgcgcctgac gccagcccca tgaagcgctc catctccacg
     8101 ctggccccgc gcccccacac ggcccggctc ggcagcaccg cgctggaccg cccagccccc
     8161 agccaggccc cgcaccatca ccaccaccgc tgccaccgcc gcagggacag gaagcagcgg
     8221 tccctggaga agggtcccag cctgtctgct gacacagatg gggcccccga cagcacggtg
     8281 gggccaggac tgcccacggg ggagggcccc cccggctgca ggcgggagcg ggagcgcagg
     8341 caggagcggg gccggtccca ggagcggagg cagccctcgt cctcctcctc ggagaagcat
     8401 cgcttctact cctgtgaccg cttcgggggc cgcgaacccc cacagcccaa gccctccctc
     8461 agtagtcacc ccacgtcgcc gacggccggg caggagccgg gaccccaccc gcaggggagt
     8521 ggttcggttc acgggagccc cctgctgtcc acatctggtg ctagcacccc gggccggggg
     8581 cggaggcagc tcccccagac ccccttgacc ccccgcccca gcgtcaccta caagacggcc
     8641 aactcctcgc ctgtccactt cgccggggca ccgagcggcc tccccgcctt ctcccccggc
     8701 cggctcagcc ggggcctctc agaacacaac gccctgctgc agagggaccc gctcagccgg
     8761 cccctggcgc ccggctcgcg catcggctcc gacccctacc tggggcagcg gctggacagc
     8821 gaggcgcccg cccgtgccct gcccgaggac gcgcccgcct ttgaggagac cgcggccagc
     8881 aactcgggcc gctcctcccg gacctcgtac gtgtcctcac tgacctcgca gcccccgccg
     8941 ctgcgccgcg tgcccaacgg ctaccactgc actctggggc tgggcggggg cggccgggcg
     9001 cggcgcggct gccaccaccc cgaccgcgac cgccggtgct agactagttc tagcaagctt
     9061 cgacgaattc gcatgcggcc gctagctcga ggtaccgata tcgagctcaa ttcactcctc
     9121 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     9181 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     9241 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     9301 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     9361 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     9421 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     9481 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     9541 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     9601 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg
     9661 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9721 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9781 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     9841 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9901 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9961 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
    10021 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
    10081 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10141 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
    10201 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    10261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    10321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    10381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    10441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    10501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    10561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    10621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    10681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    11041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    11101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    11161 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    11221 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    11281 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    11341 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    11401 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    11461 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    11521 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    11581 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    11641 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    11701 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11761 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11821 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11881 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11941 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    12001 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    12061 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    12121 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
    12181 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
    12241 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
    12301 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
    12361 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
    12421 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
    12481 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
    12541 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
    12601 ctccccaccc ccaattt
//
