LOCUS       sequence-41122-e        8515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Karpova TS, Kim MJ, Spriet C, Nalley K, Stasevich TJ, Kherrouche Z, 
            Heliot L, McNally JG
  TITLE     Concurrent fast and slow cycling of a transcriptional activator at 
            an endogenous promoter.
  JOURNAL   Science. 2008 Jan 25;319(5862):466-9.
  PUBMED    18218898
REFERENCE   2  (bases 1 to 8515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2008-01-25"; volume: "319(5862)"; pages: "466-9"
COMMENT     Sequence Label: pTSK241
FEATURES             Location/Qualifiers
     source          1..8515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1105..1818
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1825..2538
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2545..3261
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3268..3942
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNS*GTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(4152..4171)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4153..4171)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4178..4195)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4178..4194)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4187..4209)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4339..4794
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4426..4445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4636..4657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5055..5714)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(5715..5902)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(6047..6066)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6166..6188
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6226..6244)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6302..6406
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6407..6475,6476..7267)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6625..6644)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7438..8026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8180..8197
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(8350..8355,8356..8373,8374..8380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8388..8404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8393..8415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8412..8428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8412..8428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8449..8467
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     8496..8512
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8497..8513
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtagacgtat taaagctcaa tgatcctaca agacattaag taaaaagggg aagcgatctt
       61 aaatccacat gaatttcctt tctctttttc ttcttcttct gttccctttt tctttcttct
      121 tccttcaacg tctacgtaaa tataatgtat aacatctcca ctttcctttc ccttatagcg
      181 ttatgttacc tgtttaaact ctaaattctt catctactac tgctgttact acatgtatat
      241 atgttcattt tttttttttt ttttcaccac ggaaaacgta aaaatgagag catttgtaac
      301 aaataaagga gaactgttca atcaagtgac atacaaaaac tctctccaac tctacaatgg
      361 atcccgaata aacacacata aataaacaaa atggtcgtaa ttaacggggt caaatatgcc
      421 tgtgaaacgt gtatcagggg tcacagggcg gcgcagtgta ctcacactga tggtccgcta
      481 cagatgatca gacgcaaggg aagaccatcg accacatgtg gccattgtaa agagctgaga
      541 agaaccaaga acttcaaccc atccggtggg tgcatgtgtg cctctgcacg acggccagct
      601 gttggcagca aggaagatga aacacgatgt cgttgtgatg agggtgaacc ttgtaaatgt
      661 cataccaaga ggaaaagcag ccggaaatca aagggagggt catgccacag aagggcaaat
      721 gatgaagcag cgcatgtcaa tggtctcggt attgcagatc tggacgttct tttgggccta
      781 aatggtcgct cgtcggatgt agacatgaca accacattgc cgagtttgaa gccacctctg
      841 caaaacggag aaattaaggc cgacagcatt gacaatcttg atttggcttc cctcgatccg
      901 cttgagcaaa gccctagtat atctatggaa cctgttagta tcaatgaaac aggaagcgca
      961 tatacaacta cgaacacagc actaaacgat attgacattc cattctccat caatgagttg
     1021 aacgagctat acaaacaagt atcttcgcat aactcacatt cacaagcggc cgccgctgct
     1081 gccgctgcag ctgctgcagc tgcgatgtct aaaggtgaag aattattcac tggtgttgtc
     1141 ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa
     1201 ggtgaaggtg atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa
     1261 ttgccagttc catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg
     1321 agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat
     1381 gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc
     1441 aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa
     1501 gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc
     1561 atggctgaca aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa
     1621 gatggttctg ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca
     1681 gtcttgttac cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac
     1741 gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt
     1801 atggatgaat tgtacaaaga attcatgtct aaaggtgaag aattattcac tggtgttgtc
     1861 ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa
     1921 ggtgaaggtg atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa
     1981 ttgccagttc catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg
     2041 agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat
     2101 gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc
     2161 aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa
     2221 gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc
     2281 atggctgaca aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa
     2341 gatggttctg ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca
     2401 gtcttgttac cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac
     2461 gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt
     2521 atggatgaat tgtacaaatc tagaatgtct aaaggtgaag aattattcac tggtgttgtc
     2581 ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa
     2641 ggtgaaggtg atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa
     2701 ttgccagttc catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg
     2761 agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat
     2821 gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc
     2881 aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa
     2941 gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc
     3001 atggctgaca aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa
     3061 gatggttctg ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca
     3121 gtcttgttac cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac
     3181 gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt
     3241 atggatgaat tgtacaaata accgcggatg tctgttatta atttcacagg tagttctggt
     3301 ccattggtga aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc
     3361 gatgctgact tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt
     3421 attgcaagga aaatttcaag tcttgtaaaa gcatataaaa atagttaagg cactccgaaa
     3481 tacttggttg gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat
     3541 tacggcattg atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc
     3601 ctcggtttgc cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt
     3661 gcagcttcac agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca
     3721 ggtgaacttt tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa
     3781 agcttacatt ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga
     3841 ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac
     3901 tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat
     3961 ttatttaagt attgtttgtg cacttgcctg caggcctttt gaaaagcaag cataaaagat
     4021 ctaaacataa aatctgtaaa ataacaagat gtaaagataa tgctaaatca tttggctttt
     4081 tgattgattg tacaggaaaa tatacatcgc agggggttga cttttaccat ttcaccgcga
     4141 gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     4201 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccccttcg
     4261 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     4321 tgaatggcga atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4381 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4441 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4501 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4561 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4621 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4681 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4741 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcctgat
     4801 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatgat ccgtcgagtt
     4861 caagagaaaa aaaaagaaaa agcaaaaaga aaaaaggaaa gcgcgcctcg ttcagaatga
     4921 cacgtataga atgatgcatt accttgtcat cttcagtatc atactgttcg tatacatact
     4981 tactgacatt cataggtata catatataca catgtatata tatcgtatgc tgcagcttta
     5041 aataatcggt gtcactacat aagaacacct ttggtggagg gaacatcgtt ggtaccattg
     5101 ggcgaggtgg cttctcttat ggcaaccgca agagccttga acgcactctc actacggtga
     5161 tgatcattct tgcctcgcag acaatcaacg tggagggtaa ttctgctagc ctctgcaaag
     5221 ctttcaagaa aatgcgggat catctcgcaa gagagatctc ctactttctc cctttgcaaa
     5281 ccaagttcga caactgcgta cggcctgttc gaaagatcta ccaccgctct ggaaagtgcc
     5341 tcatccaaag gcgcaaatcc tgatccaaac ctttttactc cacgcgccag tagggcctct
     5401 ttaaaagctt gaccgagagc aatcccgcag tcttcagtgg tgtgatggtc gtctatgtgt
     5461 aagtcaccaa tgcactcaac gattagcgac cagccggaat gcttggccag agcatgtatc
     5521 atatggtcca gaaaccctat acctgtgtgg acgttaatca cttgcgattg tgtggcctgt
     5581 tctgctactg cttctgcctc tttttctggg aagatcgagt gctctatcgc taggggacca
     5641 ccctttaaag agatcgcaat ctgaatcttg gtttcatttg taatacgctt tactagggct
     5701 ttctgctctg tcatctttgc cttcgtttat cttgcctgct cattttttag tatattcttc
     5761 gaagaaatca cattacttta tataatgtat aattcattat gtgataatgc caatcgctaa
     5821 gaaaaaaaaa gagtcatccg ctaggtggaa aaaaaaaaat gaaaatcatt accgaggcat
     5881 aaaaaaatat agagtgtact agaggaggcc aagagtaata gaaaaagaaa attgcgggaa
     5941 aggactgtgt tatgacttcc ctgactaatg ccgtgttcaa acgatacctg gcagtgactc
     6001 ctagcgctca ccaagctctt aaaacggaat tatggtgcac tctcagtaca atctgctctg
     6061 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6121 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6181 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     6241 tatttttata ggttaatgtc atgataataa gacgtcaggt ggcacttttc ggggaaatgt
     6301 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6361 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6421 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6481 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6541 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6601 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6661 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6721 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6781 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6841 gctaaccgct ttttttcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6901 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6961 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7021 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7081 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7141 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cgggcagtca
     7201 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7261 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7321 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7381 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7441 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7501 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7561 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7621 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7681 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7741 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7801 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     7861 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7921 tccagggggg aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7981 gcgtcgattt ttgtgatgct cgtcaggggg gccgagccta tggaaaaacg ccagcaacgc
     8041 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8101 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8161 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8221 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8281 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttacctca ctcattaggc
     8341 accccaggct ttacacttta tgcttccggc tcctatgttg tgtggaattg tgagcggata
     8401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcggaa ttaaccctca
     8461 ctaaagggaa caaaagctgg gtaccgggcc ccccctcgag gtcgacggta tcgat
//
