LOCUS       sequence-41123-e        8234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Karpova TS, Kim MJ, Spriet C, Nalley K, Stasevich TJ, Kherrouche Z, 
            Heliot L, McNally JG
  TITLE     Concurrent fast and slow cycling of a transcriptional activator at 
            an endogenous promoter.
  JOURNAL   Science. 2008 Jan 25;319(5862):466-9.
  PUBMED    18218898
REFERENCE   2  (bases 1 to 8234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2008-01-25"; volume: "319(5862)"; pages: "466-9"
COMMENT     Sequence Label: pTSK368
FEATURES             Location/Qualifiers
     source          1..8234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             375..395
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             435..1148
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1155..1868
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1875..2591
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2598..3272
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNS*GTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(3482..3501)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3483..3501)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3508..3525)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3508..3524)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3517..3539)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3669..4124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3756..3775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3966..3987
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4385..5044)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(5045..5232)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(5377..5396)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5496..5518
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5556..5574)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5632..5736
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5737..5805,5806..6597)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5955..5974)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6768..7356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7510..7527
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(7680..7685,7686..7703,7704..7710)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7718..7734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7723..7745
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7742..7758
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7742..7758
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7779..7797
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     7826..7842
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7827..7843
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7883..8233
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
ORIGIN
        1 gatcccgaat ggcttctaac tttactcagt tcgttctcgt cgacaatggc ggaactggcg
       61 acgtgactgt cgccccaagc aacttcgcta acgggatcgc tgaatggatc agctctaact
      121 cgcgttcaca ggcttacaaa gtaacctgta gcgttcgtca gagctctgcg cagaatcgca
      181 aatacaccat caaagtcgag gtgcctaaag gcgcctggcg ttcgtactta aatatggaac
      241 taaccattcc aattttcgcc acgaattccg actgcgagct tattgttaag gcaatgcaag
      301 gtctcctaaa agatggaaac ccgattccct cagcaatcgc agcaaactcc ggcatctacg
      361 atgagctgta caagccaaaa aagaagagaa aggtagcggc cgccgctgct gccgctgcag
      421 ctgctgcagc tgcgatgtct aaaggtgaag aattattcac tggtgttgtc ccaattttgg
      481 ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg
      541 atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc
      601 catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg agatacccag
      661 atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa
      721 gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag
      781 gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca
      841 ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc atggctgaca
      901 aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa gatggttctg
      961 ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac
     1021 cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac gaaaagagag
     1081 accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat
     1141 tgtacaaaga attcatgtct aaaggtgaag aattattcac tggtgttgtc ccaattttgg
     1201 ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg
     1261 atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc
     1321 catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg agatacccag
     1381 atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa
     1441 gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag
     1501 gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca
     1561 ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc atggctgaca
     1621 aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa gatggttctg
     1681 ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac
     1741 cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac gaaaagagag
     1801 accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat
     1861 tgtacaaatc tagaatgtct aaaggtgaag aattattcac tggtgttgtc ccaattttgg
     1921 ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg
     1981 atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc
     2041 catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg agatacccag
     2101 atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa
     2161 gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag
     2221 gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca
     2281 ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc atggctgaca
     2341 aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa gatggttctg
     2401 ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac
     2461 cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac gaaaagagag
     2521 accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat
     2581 tgtacaaata accgcggatg tctgttatta atttcacagg tagttctggt ccattggtga
     2641 aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact
     2701 tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga
     2761 aaatttcaag tcttgtaaaa gcatataaaa atagttaagg cactccgaaa tacttggttg
     2821 gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg
     2881 atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc
     2941 cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac
     3001 agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt
     3061 tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt
     3121 ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg
     3181 ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa
     3241 tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt
     3301 attgtttgtg cacttgcctg caggcctttt gaaaagcaag cataaaagat ctaaacataa
     3361 aatctgtaaa ataacaagat gtaaagataa tgctaaatca tttggctttt tgattgattg
     3421 tacaggaaaa tatacatcgc agggggttga cttttaccat ttcaccgcga gctccaattc
     3481 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     3541 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccccttcg ccagctggcg
     3601 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3661 atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3721 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3781 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3841 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3901 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3961 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4021 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4081 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcctgat gcggtatttt
     4141 ctccttacgc atctgtgcgg tatttcacac cgcatatgat ccgtcgagtt caagagaaaa
     4201 aaaaagaaaa agcaaaaaga aaaaaggaaa gcgcgcctcg ttcagaatga cacgtataga
     4261 atgatgcatt accttgtcat cttcagtatc atactgttcg tatacatact tactgacatt
     4321 cataggtata catatataca catgtatata tatcgtatgc tgcagcttta aataatcggt
     4381 gtcactacat aagaacacct ttggtggagg gaacatcgtt ggtaccattg ggcgaggtgg
     4441 cttctcttat ggcaaccgca agagccttga acgcactctc actacggtga tgatcattct
     4501 tgcctcgcag acaatcaacg tggagggtaa ttctgctagc ctctgcaaag ctttcaagaa
     4561 aatgcgggat catctcgcaa gagagatctc ctactttctc cctttgcaaa ccaagttcga
     4621 caactgcgta cggcctgttc gaaagatcta ccaccgctct ggaaagtgcc tcatccaaag
     4681 gcgcaaatcc tgatccaaac ctttttactc cacgcgccag tagggcctct ttaaaagctt
     4741 gaccgagagc aatcccgcag tcttcagtgg tgtgatggtc gtctatgtgt aagtcaccaa
     4801 tgcactcaac gattagcgac cagccggaat gcttggccag agcatgtatc atatggtcca
     4861 gaaaccctat acctgtgtgg acgttaatca cttgcgattg tgtggcctgt tctgctactg
     4921 cttctgcctc tttttctggg aagatcgagt gctctatcgc taggggacca ccctttaaag
     4981 agatcgcaat ctgaatcttg gtttcatttg taatacgctt tactagggct ttctgctctg
     5041 tcatctttgc cttcgtttat cttgcctgct cattttttag tatattcttc gaagaaatca
     5101 cattacttta tataatgtat aattcattat gtgataatgc caatcgctaa gaaaaaaaaa
     5161 gagtcatccg ctaggtggaa aaaaaaaaat gaaaatcatt accgaggcat aaaaaaatat
     5221 agagtgtact agaggaggcc aagagtaata gaaaaagaaa attgcgggaa aggactgtgt
     5281 tatgacttcc ctgactaatg ccgtgttcaa acgatacctg gcagtgactc ctagcgctca
     5341 ccaagctctt aaaacggaat tatggtgcac tctcagtaca atctgctctg atgccgcata
     5401 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5461 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5521 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     5581 ggttaatgtc atgataataa gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5641 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5701 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5761 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5821 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5881 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5941 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6001 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6061 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6121 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6181 ttttttcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6241 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6301 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6361 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6421 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6481 ccagatggta agccctcccg tatcgtagtt atctacacga cgggcagtca ggcaactatg
     6541 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6601 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6661 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6721 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6781 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6841 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6901 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6961 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7021 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7081 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7141 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7201 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccagggggg
     7261 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7321 ttgtgatgct cgtcaggggg gccgagccta tggaaaaacg ccagcaacgc ggccttttta
     7381 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7441 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7501 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7561 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7621 gcgggcagtg agcgcaacgc aattaatgtg agttacctca ctcattaggc accccaggct
     7681 ttacacttta tgcttccggc tcctatgttg tgtggaattg tgagcggata acaatttcac
     7741 acaggaaaca gctatgacca tgattacgcc aagctcggaa ttaaccctca ctaaagggaa
     7801 caaaagctgg gtaccgggcc ccccctcgag gtcgacggta tcgatatctt cggatgcaag
     7861 ggttcgaatc ccttagctct cattattttt tgctttttct cttgaggtca catgatcgca
     7921 aaatggcaaa tggcacgtga agctgtcgat attggggaac tgtggtggtt ggcaaatgac
     7981 taattaagtt agtcaaggcg ccatcctcat gaaaactgtg taacataata accgaagtgt
     8041 cgaaaaggtg gcaccttgtc caattgaaca cgctcgatga aaaaaataag atatatataa
     8101 ggttaagtaa agcgtctgtt agaaaggaag tttttccttt ttcttgctct cttgtctttt
     8161 catctactat ttccttcgtg taatacaggg tcgtcagata catagataca attctattac
     8221 ccccatccat acag
//
