LOCUS       sequence-411294-        1638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insect expression of full-length human DCAF11. N-terminal 6X His tag
            with TEV cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pFBOH-MHL_DCAF11:1-546
FEATURES             Location/Qualifiers
     source          1..1638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(851..865)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     CDS             complement(1258..1269)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgggatcgc ggaacagcag cagtgcagga tccgggtccg gagacccctc cgagggcttg
       61 ccccgaagag gggctggcct gcgtcggagt gaggaagagg aagaagagga tgaagatgtg
      121 gatctggccc aggtactggc ctatctcctc cgcagaggcc aagtgaggtt ggtgcaggga
      181 ggaggtgcag caaatttaca attcattcag gccctcttgg actcagagga agagaatgac
      241 agagcttggg atggtcgtct tggggatcga tacaacccac ctgtggatgc tacccctgac
      301 acccgggagc tggaattcaa tgagatcaag acacaagtgg aactggccac agggcagctg
      361 gggcttaggc gggccgccca gaagcacagc tttcctcgaa tgttgcacca gagagaacgg
      421 ggcctctgcc atcggggaag cttctccctt ggagaacagt ctcgagtgat atctcacttc
      481 ttgcccaatg atctgggctt cactgatagc tactctcaga aggctttctg tggcatctac
      541 agcaaagatg gtcaaatatt catgtctgct tgccaagacc agacaatccg actctatgac
      601 tgccgatatg gccgtttcca taaattcaag agcatcaagg cccgcgatgt aggctggagc
      661 gtcttggatg tggccttcac ccctgatggg aaccacttcc tctactctag ctggtctgat
      721 tacattcata tctgcaatat ctatggtgag ggagatacac acactgccct ggatctcagg
      781 ccagatgagc gtcgctttgc tgtcttctcc attgctgtct cctcagatgg acgagaagta
      841 ctaggagggg ccaatgatgg ctgcctgtat gtctttgacc gagaacagaa ccggcgcacc
      901 cttcagattg agtcccatga ggatgatgtg aatgcagtgg cctttgctga tataagctcc
      961 caaatcctgt tctctggggg agatgatgcc atctgcaaag tgtgggatcg acgcaccatg
     1021 cgggaggatg accccaagcc tgtgggtgca ctggctggac accaggatgg catcaccttc
     1081 attgacagca agggtgatgc ccggtatctg atctccaact ctaaagacca gaccatcaaa
     1141 ctctgggata tccgacgctt ttccagccgg gaaggcatgg aagcttcacg ccaggctgcc
     1201 acacagcaaa actgggacta tcggtggcag caagtgccca aaaaagcctg gcggaagctg
     1261 aagctcccag gggacagctc cttgatgacc taccggggcc acggagtgct gcacaccctc
     1321 atccgctgcc ggttctcccc cattcatagc actggccagc agttcatcta cagtggctgc
     1381 tccactggca aagtggttgt gtacgacctt ctaagtggcc acattgtgaa gaagctgacc
     1441 aaccacaagg cctgtgtgcg tgacgtcagt tggcacccct ttgaagagaa gattgtcagc
     1501 agttcgtggg acgggaacct gcgtctgtgg cagtaccgcc aggctgagta cttccaggat
     1561 gacatgccag aatctgagga atgtgccagc gcccctgccc cagtgcccca atcctctaca
     1621 cccttttcct caccccag
//
