LOCUS       sequence-41136-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   O'Leary R, Reilly J, Hanson HH, Kang S, Lou N, Phillips GR
  TITLE     A variable cytoplasmic domain segment is necessary for 
            gamma-protocadherin trafficking and tubulation in the 
            endosome/lysosome pathway.
  JOURNAL   Mol Biol Cell. 2011 Sep 14.
  PUBMED    21917590
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2011 Sep 14."
COMMENT     Sequence Label: Delta-741-752-Delta-const-GFP
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      334..343
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(340..342,343..345,346..858)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(385..406)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(646..665)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacctgacac tctacctggt ggtggcagtg gctgtggtct cctgcatctt cctggccttt
       61 gtcattgtgc tgctggccct taggcttcga cgttggtact catcacgcct acaggcttca
      121 ggaaatggat tggctggcat accagcctct cactttgtgg gtctagatgt ttcacttacc
      181 gcaggctctc ggaagagtca cctgatcttc ccacaaccca actacgcaga cacgctcatc
      241 agccaggaga gctgtgggaa aagcgagcct cttataatac ctcaagattt acttgaaacg
      301 aaagaagacc ccacgctgcc tcagggaccg gtcgccacca tggtgagcaa gggcgaggag
      361 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      421 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      481 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      541 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      601 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      661 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      721 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      781 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      841 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaaca
//
