LOCUS       sequence-41138-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   O'Leary R, Reilly J, Hanson HH, Kang S, Lou N, Phillips GR
  TITLE     A variable cytoplasmic domain segment is necessary for 
            gamma-protocadherin trafficking and tubulation in the 
            endosome/lysosome pathway.
  JOURNAL   Mol Biol Cell. 2011 Sep 14.
  PUBMED    21917590
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2011 Sep 14."
COMMENT     Sequence Label: VCDstub-GFP
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      369..378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(375..377,378..380,381..839)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(420..441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(681..700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acctgacact ctacctggtg gtggcagtgg ctgtggtctc ctgcatcttc ctggcctttg
       61 tcattgtgct gctggccctt aggcttcgac gttggtactc atcacgccta caggcttcag
      121 gaaatggatt ggctggcata ccagcctctc actttgtggg tctagatggg gtgcaagctt
      181 tcctgcagac ctactcccag gaagtttcac ttaccgcagg ctctcggaag agtcacctga
      241 tcttcccaca acccaactac gcagacacgc tcatcagcca ggagagctgt gggaaaagcg
      301 agcctcttat aatacctcaa gatttacttg aaacgaaaga agaccccacg ctgcctcagg
      361 gaccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      421 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      481 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      541 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      661 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      721 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      781 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      841 ac
//
