LOCUS       sequence-411390-        2322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of full-length human RFWD3. C-terminal NanoLuc 
            luciferase tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pNLF1-C_RFWD3:1-774
FEATURES             Location/Qualifiers
     source          1..2322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggctcatg aagcaatgga atatgatgtt caggtgcagt taaatcatgc cgaacaacag
       61 ccagctcctg ctggcatggc cagcagccaa gggggaccag ccctcctcca gcctgttcct
      121 gctgatgtgg tcagcagcca gggggtacca tccatcctcc agccagctcc tgctgaggtg
      181 atcagcagcc aagcgacacc acccctgctc cagcctgctc cgcaactgtc tgttgacctg
      241 acagaagtgg aggtcttggg agaagacact gtggagaaca tcaatccaag aacttcagaa
      301 caacataggc agggatctga tggtaatcac accatcccag catcttcgtt gcattcaatg
      361 accaacttca tcagcggact gcagagactt catggcatgc tggaattcct gagaccttca
      421 tcttcaaacc acagtgtagg gccaatgaga acaagaagga gggtatctgc ttcacggagg
      481 gcaagagccg gagggtctca gaggacagac agtgccaggt tgagagcacc attggatgct
      541 tactttcagg tgagcaggac ccagcctgac ttgccagcta ccacttatga ttcagagact
      601 aggaatcctg tatctgaaga gttgcaggtg tctagtagtt ctgattctga cagtgacagc
      661 tctgcagagt atggaggggt tgttgaccag gcagaggaat ctggagctgt cattttagaa
      721 gagcaactag caggtgtctc agcagagcaa gaagttacat gtatcgatgg aggcaagacc
      781 ctccccaaac agccatctcc ccagaagtct gagcctctgc taccttctgc ttctatggat
      841 gaggaagaag gggacacttg tacaatatgt ctggaacagt ggaccaatgc tggggaccac
      901 cggctctcag cattacgctg tgggcatctc tttgggtata ggtgcatttc cacgtggctt
      961 aaaggacaag tacgaaaatg tccccagtgc aacaagaaag ccaggcacag tgacattgtc
     1021 gtcctttatg cccgaaccct gagagctttg gacactagtg aacaggagcg catgaaaagt
     1081 tccctactga aggaacagat gctaaggaaa caggccgagt tagaatcagc acagtgccga
     1141 ctccaactgc aggtcctcac tgataagtgc actaggcttc aaaggcgtgt tcaggacttg
     1201 caaaaactta cgtcacatca aagtcagaat ttacagcaac ccaggggctc ccaagcatgg
     1261 gtcctgagct gctcaccctc cagccagggc cagcacaagc acaagtacca cttccaaaag
     1321 accttcacag tatctcaggc aggaaactgc cggatcatgg catactgtga tgctctgagc
     1381 tgcctggtga tatcacagcc ttctcctcag gcctcttttc ttccaggctt tggtgttaag
     1441 atgttgagta ctgccaacat gaagagcagt cagtacattc cgatgcatgg caaacagatc
     1501 cgtggactgg cgtttagcag ttacctcaga ggcttgctac tctctgcttc cctagacaac
     1561 actattaaac tgaccagcct ggagacaaat accgtggtcc agacttataa tgctggacgt
     1621 cctgtctgga gctgttgctg gtgtcttgat gaggctaact acatctatgc tggactggcc
     1681 aatggttcaa ttctggtata tgacgtgcga aacacgagca gtcatgtgca ggagttagta
     1741 gctcagaaag ccagatgccc actggtctcc ctgtcataca tgcccagagc tgcctcagct
     1801 gcatttccat atggtggggt gctggctgga accttggagg atgcttcatt ctgggaacag
     1861 aaaatggact tttctcattg gcctcatgtg ctgcccttgg agccaggggg ctgcatagac
     1921 tttcagacag agaacagctc ccggcactgt cttgtgacct acaggcctga taaaaatcac
     1981 accaccatac gaagtgtgct gatggaaatg tcctaccgac tggatgacac tggaaatcca
     2041 atctgctcct gccagcctgt acatacattt tttggaggac ctacttgcaa actattgacc
     2101 aaaaatgcca ttttccaaag cccagagaat gatggcaaca tcctggtgtg tactggggat
     2161 gaagcagcaa attctgccct gctgtgggat gctgccagtg gctcgttgct ccaggaccta
     2221 cagaccgatc agcctgtgtt ggacatctgc ccatttgagg tgaaccgtaa cagctacttg
     2281 gctaccttaa cagagaagat ggtccacatc tataagtggg ag
//
