LOCUS       sequence-41140-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   O'Leary R, Reilly J, Hanson HH, Kang S, Lou N, Phillips GR
  TITLE     A variable cytoplasmic domain segment is necessary for 
            gamma-protocadherin trafficking and tubulation in the 
            endosome/lysosome pathway.
  JOURNAL   Mol Biol Cell. 2011 Sep 14.
  PUBMED    21917590
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2011 Sep 14."
COMMENT     Sequence Label: VCDstub-Delta-741-752-GFP
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      333..342
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(339..341,342..344,345..857)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(384..405)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(645..664)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acctgacact ctacctggtg gtggcagtgg ctgtggtctc ctgcatcttc ctggcctttg
       61 tcattgtgct gctggccctt aggcttcgac gttggtactc atcacgccta caggcttcag
      121 gaaatggatt ggctggcata ccagcctctc actttgtggg tctagatgtt tcacttaccg
      181 caggctctcg gaagagtcac ctgatcttcc cacaacccaa ctacgcagac acgctcatca
      241 gccaggagag ctgtgggaaa agcgagcctc ttataatacc tcaagattta cttgaaacga
      301 aagaagaccc cacgctgcct cagggaccgg tcgccaccat ggtgagcaag ggcgaggagc
      361 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      421 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      481 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      541 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      601 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      661 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      721 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      781 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      841 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag caga
//
