LOCUS       sequence-41142-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Hartley RW, Both V, Hebert EJ, Homerova D, Jucovic M, Nazarov V, 
            Rybajlak I, Sevcik J
  TITLE     Barstar inhibits extracellular ribonucleases of Streptomyces and 
            allows their production from recombinant genes.
  JOURNAL   Protein Peptide Letters 1996 3:225-231
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Peptide Letters 1996 3:225-231"
COMMENT     Sequence Label: pMT1100
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(32..37,38..53,54..60)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    68..84
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     73..95
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     sig_peptide     129..191
                     /codon_start=1
                     /product="signal sequence of E. coli alkaline phosphatase 
                     (Inouye et al., 1982)"
                     /label=phoA signal sequence
                     /translation="MKQSTIALALLPLLFTPVTKA"
ORIGIN
        1 gtattgagct gagctactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg
      181 tgacaaaagc cgcatcggtg aaggcggtgg gcagggtctg ttactcggcg ctgccctccc
      241 aggcgcacga caccctcgac ctcatagacg agggcggacc gttcccgtac tcccaggacg
      301 gcgtcgtctt ccagaaccgc gagggcctgc tgcccgccca cagcacgggc tactaccacg
      361 agtacaccgt catcaccccc ggctcgccca cgcgcggcgc ccgccggatc atcaccggtc
      421 agcagtggca ggaggactac tacaccgccg accactacgc ctccttccgc cgggtcgact
      481 tcgcctgcta acgatttcac tttccggctc tagagctcta gagtccggtc caatcagccc
      541 gcctaatgag cgggcttttt ttttgcagcc gtccgagaca ggaggacatc gtccagctga
      601 aaccggggca gaatccggcc atttctgaag agaaaaatgg ta
//
