LOCUS       sequence-41145-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Hsieh JM, Besserer GM, Madej MG, Bui HQ, Kwon S, Abramson J
  TITLE     Bridging the gap: a GFP-based strategy for overexpression and 
            purification of membrane proteins with intra and extracellular 
            C-termini.
  JOURNAL   Protein Sci. 2010 Apr;19(4):868-80.
  PUBMED    20196076
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2010-04"; volume: "19(4)"; pages: "868-80"
COMMENT     Sequence Label: pWarf(-)
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..68
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     657..677
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             730..753
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 aagaaggaga ctcgagatgt taagaattcc gggtacctgg atccttggaa gtcttgtttc
       61 agggaccatt cagcaaagga gaagactttt cactggagtt gtcccattct tgttgaatta
      121 gataaactca cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      181 cttgtcacta ctctgaccta tggtgttcaa tgcttttccc gttatccgga tcacatgaaa
      241 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg tactatatct
      301 ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt
      361 gttaatcgta tcgagttaaa gggtattgat tttaaagaag atggaaacat tctcggacac
      421 aaactagagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat
      481 ggaatcaaag ctaacttcaa aattcgccac aacattgaag atggttccgt tcaactagca
      541 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      601 tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc
      661 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaaaag
      721 cttgcggccc atcatcatca ccaccaccac cactga
//
