LOCUS       sequence-41146-e        3859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Lo L, Anderson DJ
  TITLE     A cre-dependent, anterograde transsynaptic viral tracer for mapping 
            output pathways of genetically marked neurons.
  JOURNAL   Neuron. 2011 Dec 22;72(6):938-50.
  PUBMED    22196330
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2011-12-22"; volume: "72(6)"; pages: "938-50"
COMMENT     Sequence Label: pUC57-codon-modified HTK
FEATURES             Location/Qualifiers
     source          1..3859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1638..1654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1638..1654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1651..1673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1662..1678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1686..1692,1693..1710,1711..1716))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1731..1752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1869..1886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2040..2628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2120..2139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2799..3590,3591..3659))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3422..3441
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3660..3764)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3832..3850
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tatcggatcc ctgctctaga atggcatctt atccaggaca ccagcacgca
      481 tcagcttttg accaagccgc ccgatcaaga gggcactcca atagacgtac cgcacttagg
      541 cctaggagac aacaggaagc cacagaggta agacccgagc aaaaaatgcc aacacttttg
      601 cgggtgtata tcgatggacc acacggaatg ggaaagacta ctacaaccca gctcctcgtc
      661 gcgttaggga gtcgcgacga catcgtgtac gtgcctgagc caatgaccta ctggagagtt
      721 ttaggcgcat ctgagaccat cgccaacatc tatacgacac agcacaggtt ggatcagggg
      781 gaaatctctg cgggcgatgc tgcagtggtg atgacatctg cccagatcac gatgggtatg
      841 ccttacgcag tgacagacgc agtgctggcc ccacacatcg ggggagaggc aggaagtagt
      901 cacgcccccc caccagcctt gacactgatc ttcgaccgac acccaattgc tgccttgctg
      961 tgctacccag ccgcccgata cctcatgggt tctatgaccc ctcaggcggt gctcgctttc
     1021 gttgcgctca tcccacctac actgcccggg acaaatatcg tcctgggcgc ccttccagag
     1081 gatagacata tagatagact tgctaaacgg cagagaccag gcgagcgtct cgatctggca
     1141 atgctggcgg ccataaggag ggtgtatggc cttctcgcta acactgtccg ctatctacaa
     1201 tgcggcggct cctggaggga agattggggc cagctgagcg ggaccgcagt gcccccccag
     1261 ggggcagaac ctcagtccaa cgccggacct aggccccaca ttggagatac tctgtttact
     1321 ttgttccgtg ctccagagct gctcgcgccc aatggggatc tttacaacgt atttgcttgg
     1381 gccctggacg tgctcgctaa gcggcttcgt tcaatgcacg tgtttatctt ggattatgat
     1441 cagtcacctg caggatgccg ggatgccctg cttcagctga caagtggtat ggtgcagact
     1501 cacgttacaa cacccggttc catccccacc atatgtgatc tggcaagaac atttgcacgc
     1561 gagatgggtg aggcgaactg aaagcttggg gggcccgtcg actgcagagg cctgcatgca
     1621 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1681 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     1741 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1801 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1861 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1921 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1981 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2041 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2101 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2161 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2221 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2281 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2341 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2401 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2461 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2521 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2581 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2641 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2701 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2761 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2821 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2881 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2941 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3001 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3061 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3121 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3181 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3241 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3301 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3361 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3421 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3481 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3541 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3601 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3661 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3721 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3781 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3841 atcacgaggc cctttcgtc
//
