LOCUS       sequence-411536-        4521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DCAF1. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_DCAF1:1-1507
FEATURES             Location/Qualifiers
     source          1..4521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1212..1223)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgactacag tagtggtaca tgtggactcc aaagctgagc tcactaccct gctggagcag
       61 tgggaaaagg aacatggcag tgggcaggac atggtaccta tccttaccag gatgtctcaa
      121 ttgattgaaa aagaaactga agagtatcgt aaaggggatc cagacccatt tgatgatcga
      181 catcctggtc gagctgatcc agagtgtatg ctgggccact tgctgagaat actcttcaag
      241 aatgatgatt tcatgaatgc actggtgaat gcatatgtga tgacaagccg agagccccct
      301 ttaaacactg cagcttgcag actcctatta gacatcatgc cagggctgga aactgctgtc
      361 gtctttcaag aaaaggaggg aattgtcgag aatcttttca aatgggcccg agaggccgat
      421 caaccattga ggacatattc tactggactg ttaggaggtg ctatggaaaa tcaagacatt
      481 gctgccaact atagagatga aaattcacag ctggtggcaa tagtgcttcg aagactgagg
      541 gagctacagc tacaggaagt ggctttgcgg caggaaaaca agcgtcccag tccacggaag
      601 ctctcttctg aacccctttt gcctctggat gaggaggctg tggatatgga ctatggtgac
      661 atggctgtag atgtagtgga tggagaccaa gaggaagctt ctggagacat ggagatctcc
      721 tttcatcttg attcaggcca caagactagt agcagagtga actcaacaac caaacctgag
      781 gatggaggat taaagaaaaa caagtcagca aaacagggtg acagagagaa ctttaggaaa
      841 gccaagcaaa agttgggttt ctcatcttct gatccagatc gcatgtttgt tgagctgtct
      901 aatagcagtt ggtcagaaat gtctccctgg gtgattggca ccaattatac cctttatcct
      961 atgactcctg ctatcgagca gcgactcatt ctccaatatt tgacccctct aggagaatat
     1021 caggagctac ttcccatatt catgcaactt ggatcacggg agctgatgat gttctatatt
     1081 gacctgaagc aaactaatga tgtcctgctt acatttgagg cactaaagca cctagcatct
     1141 ctcctgctcc ataacaaatt tgccacagaa tttgttgcgc atggtggagt acagaaatta
     1201 ctggaaatac ctcgtccttc tatggctgca accggtgtgt ctatgtgttt gtattaccta
     1261 tcctacaatc aggatgccat ggaaagagtt tgcatgcatc cccacaatgt tctgtctgat
     1321 gtggtgaact ataccctgtg gttaatggag tgttctcatg cttcaggatg ctgccatgct
     1381 accatgtttt tttcaatttg cttctcattt cgggccgtct tggagctctt tgaccgctat
     1441 gatggtcttc gtcgtctggt gaacttgatc agtactttgg agattctaaa tttggaagat
     1501 cagggtgcac ttctgagtga tgatgaaata tttgctagcc gccaaactgg gaaacatacc
     1561 tgcatggcct tgcgcaaata ctttgaggct cacctggcca ttaaattgga acaagtgaag
     1621 cagtcacttc agaggactga gggtggcatt cttgtccacc cacaaccccc gtacaaggca
     1681 tgctcatata ctcatgaaca gattgtggaa atgatggaat ttttgataga atatggccca
     1741 gcgcagctat attgggaacc agctgaagtt ttcctcaaac tttcttgtgt gcaactcttg
     1801 ttgcagctta tttctattgc ctgcaattgg aagacctatt atgcaaggaa tgacactgtg
     1861 cgctttgctt tggatgtcct ggctattctt actgtggtgc caaaaatcca gctccagttg
     1921 gcagaatcag tggacgtgtt ggatgaggct ggatctacag tctctactgt aggtatcagc
     1981 attattttgg gagtggctga gggtgagttc ttcatccatg atgctgaaat tcagaagtca
     2041 gcacttcaga ttatcatcaa ttgtgtgtgt ggcccagata accgaatatc cagtattggt
     2101 aaatttatct ctggtactcc tcggagaaag ctgcctcaga accctaaaag cagtgagcac
     2161 accctggcca agatgtggaa tgtggttcag tccaacaacg gcatcaaggt gctcctgtcc
     2221 ttactgtcca ttaagatgcc catcacagat gcagaccaaa tccgggccct ggcctgcaaa
     2281 gccctagtgg gcctgtctcg cagtagcact gtccggcaga tcatcagtaa actgcccctt
     2341 ttcagcagct gccagatcca gcagctgatg aaggagcctg tgctgcagga caagcgcagt
     2401 gaccatgtca agttctgcaa gtatgctgct gaactcattg aacgggtgtc aggaaaacca
     2461 cttctcattg gcactgatgt ttccctagca cgactgcaga aagcagatgt tgttgcccag
     2521 tcaaggatct ccttccctga gaaagagctg cttttgttga tacgaaacca tcttatttct
     2581 aaagggcttg gagaaacagc aaccgtgctg acaaaagagg ctgacctgcc catgactgct
     2641 gcctcccatt cttctgcctt taccccagtc actgctgctg cttctcctgt ctctctaccc
     2701 cgaacccctc gtatcgctaa tggcattgca actcgtctgg gcagccatgc tgctgtgggt
     2761 gcctctgcgc cttctgcccc tactgctcat cctcagccac ggccccccca gggtccgcta
     2821 gctctgcccg gcccatctta tgcaggcaac tcccctttga ttggtagaat cagttttatc
     2881 agagagaggc catcaccctg caatggcagg aaaatcagag tgttgcggca gaagtcggac
     2941 catggtgcct acagccaaag cccagccata aaaaaacagc tggacagaca tcttccttcc
     3001 ccacctacgc tggacagtat aatcacagag tatcttagag aacaacatgc tcgctgcaag
     3061 aatccagttg ccacctgccc acctttctcc ctctttactc ctcaccaatg tcctgagcca
     3121 aaacagaggc ggcaagcgcc aataaacttt acgtcaaggc taaaccgcag ggcatcattt
     3181 ccaaagtatg gaggggtgga tggcggatgc tttgataggc accttatctt tagcagattc
     3241 cgtcctattt cagtgttccg ggaagccaat gaagatgaga gtggcttcac ctgctgtgca
     3301 ttctcagcac gggagcggtt cctgatgctt ggcacctgca cagggcagct gaagctctat
     3361 aatgtgttta gtggacagga ggaggccagc tataactgtc acaactcagc catcacacat
     3421 cttgaacctt ccagggatgg gtccttgctg ctgacatctg ctacttggag ccagcctttg
     3481 tctgcacttt ggggaatgaa gtcagtattt gatatgaagc attccttcac agaagatcac
     3541 tatgttgagt tcagtaagca ctcccaggat cgggtcatcg gcacaaaagg agacattgcc
     3601 cacatttatg atattcagac tggcaacaag ctgttgactc tgtttaaccc agatcttgcc
     3661 aacaactaca agaggaactg tgccaccttt aatcctacag atgatcttgt cttaaatgat
     3721 ggcgtcctct gggatgtccg ctctgcacag gccatccaca agtttgacaa gttcaatatg
     3781 aacatcagtg gtgttttcca tccaaatgga ctggaggtga tcattaatac tgagatttgg
     3841 gaccttcgaa cttttcatct tttgcatact gttcccgctc tggatcagtg tcgcgtggtg
     3901 ttcaatcaca cgggaacagt gatgtatgga gctatgttgc aggcagatga tgaagatgac
     3961 ttaatggaag agaggatgaa aagccccttt gggtcatcct tccgaacatt taatgcaact
     4021 gactacaaac ctatagcaac cattgatgtg aaacggaaca tctttgacct gtgtacagac
     4081 accaaagact gctatcttgc tgtcattgag aatcaaggca gcatggatgc cctgaacatg
     4141 gacacagtat gcaggctgta tgaagtgggc aggcagcgtc tggcagagga tgaggatgaa
     4201 gaggaggacc aggaagagga agaacaggag gaagaagatg atgatgaaga tgatgatgac
     4261 accgatgatt tagatgagct tgacactgac cagttgctgg aggcggagtt ggaggaggac
     4321 gacaataatg agaacgcagg ggaagatggg gacaatgact tctctccctc tgatgaggag
     4381 ctagcaaacc ttctagagga gggagaggac ggggaggatg aagactctga tgcagatgag
     4441 gaggtggaac tgatcctggg ggacactgac agctctgaca actctgattt ggaagatgac
     4501 atcatcttat ctctgaatga g
//
