LOCUS       sequence-41170-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1182
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcggaagt tcctatacct tttgaagaat aggaacttcg
       61 gcgcgccgga gcagctctgt gccgggggct gggtggtctg gcaggatcaa ggaactggct
      121 aggaccgctg cctgctcccc tcacacactg gtgctgccgg tgctcctgca aagagagaaa
      181 ccgagtggag gggatctagg aaggctgcag tcactcagtc ccagctccct gataaaggct
      241 ctgggagggt ctaggggagc tgaggggagg ggctgagagt caccaatccc agggtggctg
      301 gaggggaggc ggggaggctg taggggggag gaggatgcag acagtagcca cacacccaga
      361 cacacacata ccccacagac ccagcagtga gaccatttcc taacagcacc cgagctggct
      421 gctgtgtatg gtttgtgtac agtttgagct ggggtcctga aatgtttgtc tcccacccca
      481 tccgagaact tagactgtac tgacagaagt cactggctac acagggaccc gtgttcatcc
      541 caaagtgagg acagcagctg ggtctcaagg cctctttatt acacgagatg ctaatgggac
      601 tcattaaaag cttctcatta cccagctaat tacagggctg tgtgggcaaa gaacatgggc
      661 cagaacccag ggttgggagc atttggtagg atgaggagag aagtgggaag ctgtttacat
      721 gcaaatggca tgactggttc attcctgtga
//
