LOCUS       sequence-41183-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Lee K, Gjorevski N, Boghaert E, Radisky DC, Nelson CM
  TITLE     Snail1, Snail2, and E47 promote mammary epithelial branching 
            morphogenesis.
  JOURNAL   EMBO J. 2011 May 24;30(13):2662-74. doi: 10.1038/emboj.2011.159.
  PUBMED    21610693
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011"; journalName: "EMBO J"; date: "2011-05-24- 24";
            volume: "30"; issue: "13"; pages: "2662-74"
COMMENT     Sequence Label: p3xFLAG-EYFP
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..103
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(122..124,125..127,128..844)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMXELYKS"
     primer_bind     complement(167..188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(428..447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     775..796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agcagagctc gtttagtgnc cgtcagaatt aaccatggac tacaaagacc atgacggtga
       61 ttataaagat catgacatcg attacaagga tgacgatgac aagcttgcgg ccgcgaattc
      121 aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      181 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      241 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      301 cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
      361 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      421 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      481 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      541 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      601 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      661 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      721 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      781 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggncgagc tgtacaagtc
      841 ctgaggatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg gncctggaag
      901 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
      961 actaggtgtc cttctatata tnatggggtg ngggggg
//
