LOCUS       sequence-411906-        3192 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of full-length human LLGL1. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_LLGL1:1-1064
FEATURES             Location/Qualifiers
     source          1..3192
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2337..2345
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgatgaagt ttcggttccg gcggcaagga gcagatccgc agcgcgagaa actgaaacaa
       61 gagttgtttg cattcaataa gaccgttgag cacggctttc ccaaccagcc tagcgcgctc
      121 gcgtttgacc cggaattgcg catcatggcc ataggtaccc gttccggcgc ggttaagatt
      181 tacggtgccc caggcgtaga gtttactgga ctccatcgcg acgcggcaac cgtgacccag
      241 atgcacttcc tgactggtca aggtcgtttg ttgtcacttc tggacgattc cagtctccat
      301 ttgtgggaaa tagtgcacca taatggttgc gcccatctcg aagaagcgtt gtctttccaa
      361 ctcccgagca ggcctggatt tgacggtgct tcagccccac tcagtcttac aagagtaact
      421 gtcgtgcttc ttgtggcggc ttccgacatt gctgcattgg gaactgaagg cagttcagtc
      481 ttctttctcg acgtaaccac cctgaccctg ctggagggcc aaaccctcgc gccaggagag
      541 gttttgcgtt cggtaccgga tgattatcgt tgtggaaagg cacttggccc agttgaatcc
      601 cttcagggac acctgcgtga cccgacaaag atacttatcg gttatagtag aggcctcctt
      661 gtcatctgga accaggcttc gcaatgtgtt gatcacatat ttctcggcaa tcaacaactt
      721 gaatcgctct gttggggaag ggatagttcc acggtcgtct cgagtcattc ggatggatcc
      781 tacgccgtat ggtcagtaga cgccggatct ttcccgacat tgcagcccac ggtcgcgaca
      841 accccttacg gaccattccc gtgtaaagcc attaataaga ttttgtggag gaactgcgag
      901 tcaggcggac acttcatcat ttttagtggc ggtatgccgc gtgcgtccta cggtgaccgc
      961 cactgcgttt cagtactgag agctgaaaca ctcgtcacct tggactttac cagtagaatt
     1021 attgactttt tcactgtaca ctccacaaga cccgaagacg aattcgacga tccccaagcg
     1081 ctcgccgtac tgctggaaga ggagcttgtg gtactcgatc ttcaaacgcc cggttggccg
     1141 gctgttccag ccccctatct ggcccccctg catagtagcg cgatcacctg cagtgcccat
     1201 gttgcgagcg tcccagcgaa gctctgggcc agaattgtca gtgcgggaga acagcaatct
     1261 ccccagccgg tatcttccgc tcttagttgg ccaattacgg gcggtcgcaa cctggcgcag
     1321 gagccgtctc aacgcggttt gttgctgacc ggtcatgaag atggtacagt acgcttctgg
     1381 gatgcatctg gtgttgcttt gaggcccctc tacaaattgt caaccgccgg cctttttcag
     1441 acagattgtg aacatgcgga ttccctggca caagctgctg aggacgattg gcctcccttc
     1501 agaaaagtgg gctgtttcga cccctatagt gatgatccga ggcttggtgt acaaaaagtt
     1561 gccttgtgta aatataccgc gcagatggtg gtagcaggca ccgccggtca agtgttggtc
     1621 cttgagctga gcgatgttcc cgtggaacag gctgtttctg ttgcgatcat agaccttttg
     1681 caggaccgtg aaggattcac ctggaaggga catgaacgtc tgtcgccgcg cacaggtcct
     1741 cttccatggc cggctggttt ccaaccccgc gtcctcgtcc aatgtcttcc tccagccgcc
     1801 gttacagcag taacgctcca tactgaatgg tcactggtgg catttggaac atctcacggc
     1861 ttcggcctct tcgactacca gcgtaagtcc ccggtactgg cgagatgcac cttgcatccg
     1921 aatgattccc tcgcgatgga gggccctctc agcagagtga aatccctgaa gaaatcactc
     1981 cgtcagtcct ttcgtaggat acgcaagagt agagtatctg gaaaaaagcg cgcggccaac
     2041 gctagttcca aacttcagga agccaatgcc caactcgcgg aacaagcctg tccccacgat
     2101 gtcgaaatga ctccagtcca gcgcaggatt gaaccacgct cagctgacga ctccctttcc
     2161 ggtgttgtta ggtgcctgta ctttgcggat acatttttgc gcgatggcgc ccatcacggc
     2221 ccgaccatgt gggcaggtac caactctggc agcgttttcg cttatgcgct tgaagtgccc
     2281 gctgccgcag tcggaggaga aaagaggcca gaacaggcgg tcgaggctgt gttgggtaag
     2341 gaggtacaac tcatgcaccg cgccccagta gttgcgattg cggtcctcga tggtcgcggc
     2401 cgtcctctcc cagaaccgta tgaggcttca agggacctcg ctcaggcccc ggacatgcaa
     2461 ggcggccacg ctgtacttat agcttcggaa gaacaattca aagtgttcac tcttccaaag
     2521 gtgagcgcca agaccaagtt caaattgacg gcacacgagg gctgcagagt tcgcaaagta
     2581 gcccttgcta ctttcgcgtc cgtagcttgc gaagactatg cagaaacctg cctggcgtgt
     2641 ttgaccaacc ttggtgacgt tcatgtcttt tctgtgcctg gactgcgccc tcaggtgcac
     2701 tactcatgta ttcgtaaaga agatatatcc ggcattgcct cctgcgtgtt cactcgccat
     2761 ggccagggct tttatttgat aagtcccagc gagttcgaga ggttctcgtt gtccgcgcgc
     2821 aacataacgg agcctctttg ttcgttggat atcaattggc ctagagacgc tacccaagcc
     2881 agttaccgta taagggagtc tcccaaactc tcacaggcca atggaacacc ttcgattctt
     2941 ttggctcctc agtccctcga cggtagccct gatccagcac atagtatggg acctgacacg
     3001 ccagagccgc ctgaggcggc cctttctcct atgagcatag attcagcaac gagtgcagat
     3061 acgactctcg atacaaccgg cgatgtgaca gtagaggatg tcaaggactt ccttggtagc
     3121 agtgaggaat ccgagaagaa tctgaggaac ctggctgaag atgaggccca cgcctgtgcc
     3181 atcctgatca aa
//
