LOCUS       sequence-411918-        6270 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of full-length human NUP214. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_NUP214:1-2090
FEATURES             Location/Qualifiers
     source          1..6270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      488..497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3146..3157
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgggagacg agatggatgc catgattccc gagcgggaga tgaaggattt tcagtttaga
       61 gcgctaaaga aggtgagaat ctttgactcc cctgaggaat tgcccaagga acgctcgagt
      121 ctgcttgctg tgtccaacaa atatggtctg gtcttcgctg gtggagccag tggcttgcag
      181 atttttccta ctaaaaatct tcttattcaa aataaacccg gagatgatcc caacaaaata
      241 gttgataaag tccaaggctt gctagttcct atgaaattcc caatccatca cctggccttg
      301 agctgtgata acctcacact ctctgcgtgc atgatgtcca gtgaatatgg ttccattatt
      361 gctttttttg atgttcgcac attctcaaat gaggctaaac agcaaaaacg cccatttgcc
      421 tatcataagc ttttgaaaga tgcaggaggc atggtgattg atatgaagtg gaaccccact
      481 gtcccctcca tggtggcagt ttgtctggct gatggtagta ttgctgtcct gcaagtcacg
      541 gaaacagtga aagtatgtgc aactcttcct tccacggtag cagtaacctc tgtgtgctgg
      601 agccccaaag gaaagcagct ggcagtggga aaacagaatg gaactgtggt ccagtatctt
      661 cctactttgc aggaaaaaaa agtcattcct tgtcctccgt tttatgagtc agatcatcct
      721 gtcagagttc tggatgtgct gtggattggt acctacgtct tcgccatagt gtatgctgct
      781 gcagatggga ccctggaaac gtctccagat gtggtgatgg ctctactacc gaaaaaagaa
      841 gaaaagcacc cagagatatt tgtgaacttt atggagccct gttatggcag ctgcacggag
      901 agacagcatc attactacct cagttacatt gaggaatggg atttagtgct ggcagcatct
      961 gcggcttcaa cagaagttag tatccttgct cgacaaagtg atcagattaa ttgggaatct
     1021 tggctactgg aggattctag tcgagctgaa ttgcctgtga cagacaagag tgatgactcc
     1081 ttgcccatgg gagttgtcgt agactataca aaccaagtgg aaatcaccat cagtgatgaa
     1141 aagactcttc ctcctgctcc agttctcatg ttactttcaa cagatggtgt gctttgtcca
     1201 ttttatatga ttaatcaaaa tcctggggtt aagtctctca tcaaaacacc agagcgactt
     1261 tcattagaag gagagcgaca gcccaagtca ccaggaagta ctcccactac cccaacctcc
     1321 tctcaagccc cacagaaact ggatgcttct gcagctgcag cccctgcctc tctgccacct
     1381 tcatcacctg ctgctcccat tgccactttt tctttgcttc ctgctggtgg agcccccact
     1441 gtgttctcct ttggttcttc atctttgaag tcatcggcta cggtcactgg ggagccccct
     1501 tcatattcca gtggctccga cagctccaaa gcagccccag gccctggccc atcaaccttc
     1561 tcttttgttc ccccttctaa agcctcccta gcccccaccc ctgcagcgtc tcctgtggct
     1621 ccatcagctg cttcattctc ctttggatca tctggtttta agcctaccct ggaaagcaca
     1681 ccagtgccaa gtgtgtctgc tccaaatata gcaatgaagt cctccttccc accctcaacc
     1741 tctgctgtca aagtcaacct tagtgaaaag tttactgctg cagctacctc tactcctgtt
     1801 agtagctccc agagcgcacc cccgatgtcg ccattctctt ctgcctccaa gccagctgct
     1861 tctggaccac tcagccaccc cacacctctc tcagcaccac ctagttccgt gccattgaag
     1921 tcctcagtct tgccctcacc atcaggacga tctgctcagg gcagttcaag cccagtgccc
     1981 tcaatggtac agaaatcacc caggataacc cctccagcgg caaagccagg ctctccccag
     2041 gcaaagtcac ttcagcctgc tgttgcagaa aagcagggac atcagtggaa agattcagat
     2101 cctgtaatgg ctggaattgg ggaggagatt gcacactttc agaaggagtt ggaagagtta
     2161 aaagcccgaa cttccaaagc ctgtttccaa gtgggcactt ctgaggagat gaagatgctg
     2221 cgaacagaat cagatgactt gcataccttt cttttggaga ttaaagagac cacagagtcg
     2281 cttcatggag atataagtag cctgaaaaca actttacttg agggctttgc tggtgttgag
     2341 gaagccagag aacaaaatga aagaaatcgt gactctggtt atctgcattt gctttataaa
     2401 agaccactgg atcccaagag tgaagctcag cttcaggaaa ttcggcgcct tcatcagtat
     2461 gtgaaatttg ctgtccaaga tgtgaatgat gttctagact tggagtggga tcagcatctg
     2521 gaacaaaaga aaaaacaaag gcacctgctt gtgccagagc gagagacact gtttaacacc
     2581 ctagccaaca atcgggaaat catcaaccaa cagaggaaga ggctgaatca cctggtggat
     2641 agtcttcagc agctccgcct ttacaaacag acttccctgt ggagcctgtc ctcggctgtt
     2701 ccttcccaga gcagcattca cagttttgac agtgacctgg aaagcctgtg caatgctttg
     2761 ttgaaaacca ccatagaatc tcacaccaaa tccttgccca aagtaccagc caaactgtcc
     2821 cccatgaaac aggcacaact gagaaacttc ttggccaaga ggaagacccc accagtgaga
     2881 tccactgctc cagccagcct gtctcgatca gcctttctgt ctcagagata ttatgaagac
     2941 ttggatgaag tcagctcaac gtcatctgtc tcccagtctc tggagagtga agatgcacgg
     3001 acgtcctgta aagatgacga ggcagtggtt caggcccctc ggcacgcccc cgtggttcgc
     3061 actccttcca tccagcccag tctcttgccc catgcagcac cttttgctaa atctcacctg
     3121 gttcatggtt cttcacctgg tgtgatggga acttcagtgg ctacatctgc tagcaaaatt
     3181 attcctcaag gggccgatag cacaatgctt gccacgaaaa ccgtgaaaca tggtgcacct
     3241 agtccttccc accccatctc agccccgcag gcagctgccg cagcagcact caggcggcag
     3301 atggccagtc aggcaccagc tgtaaacact ttgactgaat caacgttgaa gaatgtccct
     3361 caagtggtaa atgtgcagga attgaagaat aaccctgcaa ccccttctac agccatgggt
     3421 tcttcagtgc cctactccac agccaaaaca cctcacccag tgttgacccc agtggctgct
     3481 aaccaagcca agcaggggtc tctaataaat tcccttaagc catctgggcc tacaccagca
     3541 tccggtcagt tatcatctgg tgacaaagct tcagggacag ccaagataga aacagctgtg
     3601 acttcaaccc catctgcttc tgggcagttc agcaagcctt tctcattttc tccatcaggg
     3661 actggcttta attttgggat aatcacacca acaccgtctt ctaatttcac tgctgcacaa
     3721 ggggcaacac cctccactaa agagtcaagc cagccggacg cattctcatc tggtggggga
     3781 agcaaacctt cttatgaggc cattcctgaa agctcacctc cctcaggaat cacatccgca
     3841 tcaaacacca ccccaggaga acctgccgca tctagcagca gacctgtggc accttctgga
     3901 actgctcttt ccaccacctc tagtaagctg gaaaccccac cgtccaagct gggagagctt
     3961 ctgtttccaa gttctttggc tggagagact ctgggaagtt tttcaggact gcgggttggc
     4021 caagcagatg attctacaaa accaaccaat aaggcttcat ccacaagcct aactagtacc
     4081 cagccaacca agacgtcagg cgtgccctca gggtttaatt ttactgcccc cccggtgtta
     4141 gggaagcaca cggagccccc tgtgacatcc tctgcaacca ccacctcagt agcaccacca
     4201 gcagccacca gcacttcctc aactgccgtt tttggcagtc tgccagtcac cagtgcagga
     4261 tcctctgggg tcatcagttt tggtgggaca tctctaagtg ctggcaagac tagtttttca
     4321 tttggaagcc aacagaccaa tagcacagtg cccccatctg ccccaccacc aactacagct
     4381 gccactcccc ttccaacatc attccccaca ttgtcatttg gtagcctcct gagttcagca
     4441 actaccccct ccctgcctat gtccgctggc agaagcacag aagaggccac ttcatcagct
     4501 ttgcctgaga agccaggtga cagtgaggtc tcagcatcag cagcctcact tctagaggag
     4561 caacagtcag cccagcttcc ccaggctcct ccgcaaactt ctgactctgt taaaaaagaa
     4621 cctgttcttg cccagcctgc agtcagcaac tctggcactg cagcatctag tactagtctt
     4681 gtagcacttt ctgcagaggc taccccagcc accacggggg tccctgatgc caggacggag
     4741 gcagtaccac ctgcttcctc cttttctgtg cctgggcaga ctgctgtcac agcagctgct
     4801 atctcaagtg caggccctgt ggccgtcgaa acatcaagta cccccatagc ctccagcacc
     4861 acgtccattg ttgctcccgg cccatctgca gaggcagcag catttggtac cgtcacttct
     4921 ggctcatccg tctttgctca gcctcctgct gccagttcta gctcagcttt caaccagctc
     4981 accaacaaca cagccactgc cccctctgcc acgcccgtgt ttgggcaagt ggcagccagc
     5041 accgcaccaa gtctgtttgg gcagcagact ggtagcacag ccagcacagc agctgccaca
     5101 ccacaggtca gcagctcagg gtttagcagc ccagcttttg gtaccacagc cccaggggtc
     5161 tttggacaga caaccttcgg gcaggcctca gtctttgggc agtcggcgag cagtgctgca
     5221 agtgtctttt ccttcagtca gcctgggttc agttccgtgc ctgccttcgg tcagcctgct
     5281 tcctccactc ccacatccac cagtggaagt gtctttggtg ccgcctcaag taccagtagc
     5341 tccagttcct tctcatttgg acagtcttct cccaacacag gaggggggct gtttggccaa
     5401 agcaacgctc ctgcttttgg gcagagtcct ggctttggac agggaggctc tgtctttggt
     5461 ggtacctcag ctgccaccac aacagcagca acctctgggt tcagcttttg ccaagcttca
     5521 ggttttgggt ctagtaatac tggttctgtg tttggtcaag cagccagtac tggtggaata
     5581 gtctttggcc agcaatcatc ctcttccagt ggtaacgtgt ttgggtctgg aaacactgga
     5641 agagggggag gtttcttcag tggccttgga ggaaaaccca gtcaggatgc agccaacaaa
     5701 aacccattca gctcggccag tgggggcttt ggatccacag ctacctcaaa tacctctaac
     5761 ctatttggaa acagtggggc caagacattt ggtggatttg ccagctcgtc gtttggagag
     5821 cagaaaccca ctggcacttt cagctctgga ggaggaagtg tggcatccca aggctttggg
     5881 ttttcctctc caaacaaaac aggtggcttc ggtgctgctc cagtgtttgg cagccctcct
     5941 acttttgggg gatcccctgg gtttggaggg gtgccagcat tcggttcagc cccagccttt
     6001 acaagccctc tgggctcgac gggaggcaaa gtgttcggag agggcactgc agctgccagc
     6061 gcaggaggat tcgggtttgg gagcagcagc aacaccacat ccttcggcac gctcgcgagt
     6121 cagaatgccc ccactttcgg atcactgtcc caacagactt ctggttttgg gacccagagt
     6181 agcggattct ctggttttgg atcaggcaca ggagggttca gctttgggtc aaataactcg
     6241 tctgtccagg gttttggtgg ctggcgaagc
//
