LOCUS       sequence-411968-        2157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human NWD2 fragment S944 to S1662. 
            N-terminal HiBiT tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_NWD2:944-1662
FEATURES             Location/Qualifiers
     source          1..2157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      913..922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccattgtac cactgcattc atccatggat gtgacataca gcccagagcg tcttccctta
       61 tcatccagtc acctgcatgt cacagagatc ctgcctacct gtaaccccag cactgtcctc
      121 acagctttag aaaatggttc catcagcacc tgggatgtag agactcgaca gctactcagg
      181 caaatcacca cagcccagtc tgtcatcctg ggcatgaaac tcaccagtga tgaaaagtac
      241 cttgtggtgg ctacaacaaa taacaccttg ttgatttatg acaatgtcaa ttcttgcctc
      301 ctgtctgaag tagaaatcaa ggggaccaag catggaagca gcgccaccta catcaatgga
      361 tttacactgt ccgccaacca cgcccttgca tggctcgaag ccagcaaaga tgtcactgtc
      421 atcgatctgc tgtacggatg gccgctttac cagttccact gctggtatga agtgacgtgc
      481 gtccagtgct ccctggatgg tctgtatgct ttctgtggcc aatacctgaa cacaaccacc
      541 atatttcatt tagggagtgg agaaaagtta tgtacagtga catcagaatt ttcaggtgga
      601 tttgtgaagt ttcttcttat cttggacaca gctcaggaaa tggtcatggt agacagtgaa
      661 ggaagtcttt ctgtttggaa tactgaggac atttccagcc cccagctgac tgatgacttt
      721 gattgccgaa gagaagacag tgaggtggtc agcattgagc tttcagaaga ccaaagtgca
      781 gttctgatct gtaaagccct cagcattgag ctcttagata ctggtctgtg gaaggtggct
      841 gaaaagttca gagccaagca caacgaacgc tttatatctg ccgtgctgtc taaaaatgga
      901 gattgtatca tcgccaccat ggaaaatacc tcagctgtgt ttttttggag gcgggacaca
      961 ggacagtgta tggcaagctt gcaggaaatc tcaggttcca ttgttaagtt agtgaaatcc
     1021 agtcaccaca atatgctact gtctttatca accagtggtg ttctttccat ttgggacata
     1081 gatataatca cagctatgtc caacatagat aagactggaa aacccatcca aagtctgttg
     1141 ttgcctgcta gaggggaaat catttactcc ctggatggat ccgattgtgt tcataagtgg
     1201 aacttcagca gtggcttcat cgaagcagta ttcaagcatg aaggaatagt tgaacactgt
     1261 gtgttaacat ccaccggaga tataatggtg acatcagatg acaaaagcag ccagtatgtc
     1321 tggcacacca gcagtggtga aaaccttttt cgaattaacg ggcagagaat atctcagctg
     1381 ctgattacac acaatgacca gtttgtggtc tcgctctgtg aggaaaatgc ctccagggtt
     1441 tggaggctcg ccacaggcca cagggtctgc aacattctga ccactttgca gaatgccttt
     1501 attacctccg caaatacctt cgtggtggga atgactaaaa gcaaagtgtt ggcagtcagt
     1561 ctttggacag gaagtatcac caagaaattt tgctgtgaag atgggaccac catcgtgaat
     1621 tttaaattaa tccctgactg tcctgatatc atcgtgttta tcacatcggc cgagactgtg
     1681 aacatctgga gtctgacaga tgaagtgatc tgtcggcgcg tgcaacttcc aaacaacttc
     1741 ttgaaaaatc tggaggactt tgaaatttct cccaatggaa agctaggcat tatagccagg
     1801 ggagatgaaa acatcaatgt gctagattta tacagtggta aattgcgggt ggttcacgcc
     1861 tctgggatca tctggcggca gaggttgtct cgggatggtc gctacctggt atacatttgt
     1921 ttccgaaatg gggaggagga ggatgaaaat ggtgcaatat tcagtttaat tgtaatgaga
     1981 ctggcagatg gcaaaaatat cggtgcttgt tccctttaca aaacaccaac tttccttgca
     2041 ctctcccaga ggcacctgaa catcattgtg ggctttgatg atgggagtat agggatctac
     2101 acggtagtag accgtgtaga tgctgcactg aaaatcaaaa ttgccacttc aaatagc
//
