LOCUS       sequence-412150-        8657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent neurotensin (NTS) 
            sensor GRAB_NTS1.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Wang H, Qian T, Zhao Y, Zhuo Y, Wu C, Osakada T, Chen P, Chen Z, Ren
            H, Yan Y, Geng L, Fu S, Mei L, Li G, Wu L, Jiang Y, Qian W, Zhang L,
            Peng W, Xu M, Hu J, Jiang M, Chen L, Tang C, Zhu Y, Lin D, Zhou JN, 
            Li Y
  TITLE     A tool kit of highly selective and sensitive genetically encoded 
            neuropeptide sensors.
  JOURNAL   Science. 2023 Nov 17;382(6672):eabq8173. doi: 
            10.1126/science.abq8173. Epub 2023 Nov 17.
  PUBMED    37972184
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-11-17"; volume: "382(6672):eabq8173. doi"; pages: " 
            10.1126/science.abq8173. Epub 2023 Nov 17"
COMMENT     Sequence Label: pDisplay-GRAB_NTS1.0
FEATURES             Location/Qualifiers
     source          1..8657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     722..781
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    785..809
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1915..1936
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2042..2063)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2303..2322)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    join(2856..3429,3430..3432,3433..3441)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3023..3040)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3249..3268
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3442..4149
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3587..3605)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3855..3874
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4207..4236
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4240..4386
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4408..4425)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4414..4638
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4770..5358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4850..4869)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5687..5734
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5759..5778
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5966..6760)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6077..6096)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6687..6706
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6859..6878)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6957..7148
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7105..7125
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7208..7999,8000..8068))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7831..7850
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8069..8173)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8200..8655
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8287..8306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8497..8518
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcac
      721 catggagaca gacacactcc tgctatgggt actgctgctc tgggttccag gttccactgg
      781 tgacacaagt ttgtacaaaa aagttggcac caccggtcgc ctcaacagct ccgcgccggg
      841 aaccccgggc acgccggccg ccgacccctt ccagcgggcg caggccggac tggaggaggc
      901 gctgctggcc ccgggcttcg gcaacgcttc gggcaacgcg tcggagcgcg tcctggcggc
      961 acccagcagc gagctggacg tgaacaccga catctactcc aaggtgctgg tgaccgccgt
     1021 gtacctggcg ctcttcgtgg tgggcacggt gggcaacacg gtgacggcgt tcacgctggc
     1081 gcggaagaag tcgctgcaga gcctgcagag cacggtgcat taccacctgg gcagcctggc
     1141 gctgtccgac ctgctcaccc tgctgctggc catgcccgtg gagctgtaca acttcatctg
     1201 ggtgcaccac ccctgggcct tcggcgacgc cggctgccgc ggctactact tcctgcgcga
     1261 cgcctgcacc tacgccacgg ccctcaacgt ggccagcctg agtgtggagc gctacctggc
     1321 catctgccac cccttcaagg ccaagaccct catgtcccga agccgcacca agaagttcat
     1381 cagcgccatc tggctcgcct cggccctgct ggcggtgcct atgctgttca ccatgggcga
     1441 gcagaaccgc agcgccgacg gccagcacgc cggcggcctg gtgtgcaccc ccaccatcca
     1501 cactgccacc gtcaaggtcg tcatacaggt caacaccttc atgtccttca tattccccat
     1561 ggtggtcatc tcggtcctga acaccatcat cgccaacaag ctgaccgtca tggtacgcca
     1621 ggcggccgag cagctcaacg gcgcccctgg cgagcccgcg ccggccgggc cgcgcgacac
     1681 cgacgcgctg gacctggagg agggaggaaa cgtctatatc aaggccgaca agcagaagaa
     1741 cggcatcaag gcggcgttca atatccgcca caacatcgag gacggcggcg tgcagctcgc
     1801 ctaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1861 ctacctgagc gtgcagtcca aactttcgaa agaccccaac gagaagcgcg atcacatggt
     1921 cctgctggag ttcgtgaccg ccgccgggat cacttggggc atggacgagc tgtacaaggg
     1981 cggtaccgga gggagcatgg tgagaaaggg cgaggagctg ttcaccgggg tggtgcccat
     2041 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgggcg gcgagggtga
     2101 gggcgatgcc accgagggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2161 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2221 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca
     2281 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2341 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2401 caacatcctg gggcacaagc tggagtacaa caccggagca gcagcacgct ggcgcgggcg
     2461 ggagcctaat agggtccagg ccctgcggca cggcgtgcgc gtcctacgtg cagtggtcat
     2521 cgcctttgtg gtctgctggc tgccctacca cgtgcggcgc ctcatgttct gctacatctc
     2581 ggatgagcag tggactccgt tcctctatga cttctaccac tacttctaca tggtgaccaa
     2641 cgcactcttc tacgtcagct ccaccatcaa ccccatcctg tacaacctcg tctctgccaa
     2701 cttccgccac atcttcctgg ccacactggc ctgcctctgc ccggtgtggc ggcgcaggag
     2761 gaagaggcca gccttctcga ggaaggccga cagcgtgtcc agcaaccaca ccctctccag
     2821 caatgccacc cgcgagacgc tgtactaggc tagcgcccct ctccctcccc cccccctaac
     2881 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     2941 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3001 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3061 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3121 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaacgcca cgtgtataag
     3181 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     3241 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     3301 cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga
     3361 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     3421 gatgataata tggccacaac catggtgagc aagggcgagg aggataacat ggccatcatc
     3481 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     3541 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     3601 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     3661 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     3721 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     3781 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     3841 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     3901 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     3961 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     4021 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     4081 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     4141 ctgtacaagg atggtaaaaa gaagaaaaag aagtcaaaga caaagtgtgt aattatgtaa
     4201 gtcgacgaac aaaaactcat ctcagaagag gatctgaatg ctgtgggcca ggacacgcag
     4261 gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg tggtgatctc agccatcctg
     4321 gccctggtgg tgctcaccat catctccctt atcatcctca tcatgctttg gcagaagaag
     4381 ccacgttagg cggccgctcg agatcagcct cgactgtgcc ttctagttgc cagccatctg
     4441 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4501 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4561 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4621 atgcggtggg ctctatggct tctgaggcgg aaagaaccag tggcggtaat acggttatcc
     4681 acagaatcgg gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4741 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4801 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4861 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4921 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4981 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5041 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacgc
     5101 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5161 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5221 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5281 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5341 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5401 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5461 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaacctgag
     5521 gctatggcag ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc acccgaactt
     5581 ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt
     5641 ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac aaacgaccca
     5701 acaccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt
     5761 gtctccttcc gtgtttcagt tagcctcccc ctagggtggg cgaagaactc cagcatgaga
     5821 tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt
     5881 tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg
     5941 gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     6001 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     6061 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     6121 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     6181 gcaagcaggc atcgccatag gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga
     6241 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     6301 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     6361 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     6421 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     6481 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     6541 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg
     6601 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     6661 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     6721 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     6781 tctcttgatc gatctttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     6841 aatagctcag aggccgggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     6901 gcggagaatg ggcggaactg ggcggagtta ggggcggaat gggcggagtt aggggcggga
     6961 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     7021 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     7081 ctggggagcc tggggacttt ccacacccta actgacacac attccacagc tggttctttc
     7141 cgcctcagga ctcttccttt ttcaataaat caatctaaag tatatatgag taaacttggt
     7201 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7261 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7321 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7381 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7441 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7501 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7561 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7621 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7681 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7741 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7801 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7861 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7921 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7981 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8041 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8101 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8161 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     8221 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8281 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8341 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8401 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8461 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8521 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8581 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     8641 taacgcttac aatttac
//
