LOCUS       sequence-412165-        8723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) 
            control sensor GRAB_NPYmut in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Wang H, Qian T, Zhao Y, Zhuo Y, Wu C, Osakada T, Chen P, Chen Z, Ren
            H, Yan Y, Geng L, Fu S, Mei L, Li G, Wu L, Jiang Y, Qian W, Zhang L,
            Peng W, Xu M, Hu J, Jiang M, Chen L, Tang C, Zhu Y, Lin D, Zhou JN, 
            Li Y
  TITLE     A tool kit of highly selective and sensitive genetically encoded 
            neuropeptide sensors.
  JOURNAL   Science. 2023 Nov 17;382(6672):eabq8173. doi: 
            10.1126/science.abq8173. Epub 2023 Nov 17.
  PUBMED    37972184
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-11-17"; volume: "382(6672):eabq8173. doi"; pages: " 
            10.1126/science.abq8173. Epub 2023 Nov 17"
COMMENT     Sequence Label: pDisplay-GRAB_NPYmut
FEATURES             Location/Qualifiers
     source          1..8723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     737..796
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1975..1996
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2102..2123)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2363..2382)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    join(2922..3495,3496..3498,3499..3507)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3089..3106)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3315..3334
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3508..4215
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3653..3671)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3921..3940
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4273..4302
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4306..4452
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4474..4491)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4480..4704
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4836..5424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4916..4935)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5753..5800
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5825..5844
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6032..6826)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6143..6162)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6753..6772
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6925..6944)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        7023..7214
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7171..7191
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7274..8065,8066..8134))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7897..7916
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8135..8239)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8266..8721
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8353..8372)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8563..8584
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtaattcaac
      841 attattttcc caggttgaaa atcattcagt ccactctaat ttctcagaga agaatgccca
      901 gcttctggct tttgaaaatg atgattgtca tctgcccttg gccatgatat ttaccttagc
      961 tcttgcttat ggagctgtga tcattcttgg tgtctctgga aacctggcct tgatcataat
     1021 catcttgaaa caaaaggaga tgagaaatgt taccaacatc ctgattgtga acctttcctt
     1081 ctcagacttg cttgttgcca tcacgtgtct cccctttaca tttgtctaca cattaatgga
     1141 ccactgggtc ttcggtgagg cgatgtgtaa gttgaatcct tttgtgaggt gtgtttcaat
     1201 cactgtgtcc attttctctc tggttctcat tgctgtggaa cgacatcagc tgataatcaa
     1261 ccctcgaggg tggagaccaa ataatagaca tgcttatgta ggtattgctg tgatttgggt
     1321 ccttgctgtg gcttcttctt tgcctttcct gatctaccaa gtaatgactg atgagccgtt
     1381 ccaaaatgta acacttgatg cgtacaaaga caaatacgtg tgctttgatc aatttccatc
     1441 ggactctcat aggttgtctt ataccactct cctcttggtg ctgcagtatt ttggtccact
     1501 ttgttttata tttatttgct acttcaagat atatatacgc ctaaaaagga gaaacaacat
     1561 gatgccaccc agccgccggg gtccggacgc cgtcgccgcg ccgccggggg gcaccgagcg
     1621 caggcccaac ggtctgggcc ccgagcgcag cgcgggcccg gggggcgcag aggccgaacc
     1681 gctgcccacc cagctcaacg gcgcccctgg cgagcccgcg ccggccgggc cgcgcgacac
     1741 cgacgcgctg gacctggagg agggaggaaa cgtctatatc aaggccgaca agcagaagaa
     1801 cggcatcaag gcgaacttcg gtatccgcca caacatcgag gacggcggcg tgcagctcgc
     1861 ctaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1921 ctacctgagc gtgcagtcca aactttcgaa agaccccaac gagaagcgcg atcacatggt
     1981 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtaccgcgg
     2041 cggtaccgga gggagcatgg tgagaaaggg cgaggagctg ttcaccgggg tggtgcccat
     2101 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga
     2161 gggcgatgcc accgagggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2221 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2281 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca
     2341 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2401 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2461 caacatcctg gggcacaagc tggagtacaa caccggagca gcagcacgct ggcgcgggcg
     2521 gcagaacgaa accaaaagaa tcaatatcat gctgctctcc attgtggtag catttgcagt
     2581 ctgctggctc cctcttacca tctttaacac tgtgtttgat tggaatcatc agatcattgc
     2641 tacctgcaac cacaatctgt tattcctgct ctgccacctc acagcaatga tgtccacttg
     2701 tgtcaacccc atattttatg ggttcctgaa caaaaacttc cagagagact tgcagttctt
     2761 cttcaacttt tgtgatttcc ggtctcggga tgatgattat gaaacaatag ccatgtccac
     2821 gatgcacaca gatgtttcca aaacttcttt gaagcaagca agcccagtcg catttaaaaa
     2881 aatcaacaac aatgatgata atgaaaaaat ctaagctagc gcccctctcc ctcccccccc
     2941 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     3001 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     3061 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     3121 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     3181 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca acgccacgtg
     3241 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     3301 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     3361 aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt
     3421 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     3481 aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagga taacatggcc
     3541 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     3601 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     3661 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     3721 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     3781 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     3841 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     3901 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     3961 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     4021 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     4081 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     4141 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     4201 gacgagctgt acaaggatgg taaaaagaag aaaaagaagt caaagacaaa gtgtgtaatt
     4261 atgtaagtcg acgaacaaaa actcatctca gaagaggatc tgaatgctgt gggccaggac
     4321 acgcaggagg tcatcgtggt gccacactcc ttgcccttta aggtggtggt gatctcagcc
     4381 atcctggccc tggtggtgct caccatcatc tcccttatca tcctcatcat gctttggcag
     4441 aagaagccac gttaggcggc cgctcgagat cagcctcgac tgtgccttct agttgccagc
     4501 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4561 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4621 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4681 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagtggc ggtaatacgg
     4741 ttatccacag aatcggggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4801 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4861 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4921 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4981 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5041 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5101 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5161 agacgcgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5221 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5281 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5341 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5401 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5461 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5521 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5581 cctgaggcta tggcagggcc tgccgccccg acgttggctg cgagccctgg gccttcaccc
     5641 gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt
     5701 ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac
     5761 gacccaacac cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc
     5821 ggtattgtct ccttccgtgt ttcagttagc ctccccctag ggtgggcgaa gaactccagc
     5881 atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc
     5941 aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct
     6001 tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga
     6061 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     6121 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     6181 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     6241 tattcggcaa gcaggcatcg ccataggtca cgacgagatc ctcgccgtcg ggcatgctcg
     6301 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     6361 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     6421 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     6481 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     6541 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     6601 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg
     6661 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     6721 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     6781 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc
     6841 atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     6901 ttctggaata gctcagaggc cgggcctcgg cctctgcata aataaaaaaa attagtcagc
     6961 catggggcgg agaatgggcg gaactgggcg gagttagggg cggaatgggc ggagttaggg
     7021 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     7081 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     7141 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt
     7201 tctttccgcc tcaggactct tcctttttca ataaatcaat ctaaagtata tatgagtaaa
     7261 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7321 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7381 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7441 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7501 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7561 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7621 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7681 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7741 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7801 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7861 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7921 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7981 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8041 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8101 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8161 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8221 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     8281 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8341 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8401 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8461 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8521 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8581 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8641 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     8701 aaatattaac gcttacaatt tac
//
