LOCUS       sequence-412224-       12407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with a N-terminal GFP 
            tag and a mutation (W391A) in the ?1-interacting domain (AID) in the
            I-II loop of Cav2.2 that prevents Beta subunit binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12407)
  AUTHORS   Waithe D, Ferron L, Page KM, Chaggar K, Dolphin AC
  TITLE     Beta-subunits promote the expression of Ca(V)2.2 channels by 
            reducing their proteasomal degradation.
  JOURNAL   J Biol Chem. 2011 Mar 18;286(11):9598-611. doi: 
            10.1074/jbc.M110.195909. Epub 2011 Jan 13.
  PUBMED    21233207
REFERENCE   2  (bases 1 to 12407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.195909"; journalName: "J Biol Chem"; date: 
            "2011-03-18- 18"; volume: "286"; issue: "11"; pages: "9598-611"
COMMENT     Sequence Label: GFP Cav2.2 W391A pRK5
FEATURES             Location/Qualifiers
     source          1..12407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        1020..1399
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1400..1603
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1554..1574
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1600..1624
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1834..1852
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1834..1851
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1865..1884
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1960..2673
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1960..2673
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1990..2018)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2613..2633
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     regulatory      9713..9722
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9726..9860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9763..9782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9817..9836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        9929..10286
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9929..10120
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9952..9972)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10137..10272
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10306..10323)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10306..10322)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10315..10337)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10535..10990
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10622..10641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10832..10853
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11007..11026)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11126..11148
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11186..11204)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11272..11376
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11377..11445,11446..12237)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11595..11614)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga catgattacg aattaattcg agctcgcccg
     1021 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     1081 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1141 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1201 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1261 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1321 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     1381 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     1441 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     1501 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     1561 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     1621 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     1681 cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca agagtgacgt
     1741 aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac gcggctacaa
     1801 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaataacatc
     1861 cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct cggttctatc
     1921 gattgaattc ctcgagggta ccgagctcgg atctaaaaaa tgagtaaagg agaagaactt
     1981 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     2041 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     2101 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
     2161 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
     2221 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     2281 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     2341 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     2401 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     2461 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     2521 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     2581 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     2641 gggattacac atggcatgga tgaactatac aaaatggtcc gcttcgggga cgagctgggc
     2701 ggccgctatg ggggcgcggg cggcgcggag cgggcccggg gcggcggggc cggcggggct
     2761 ggcggcccgg gccccggggg gctgccgccg ggccagcggg tcctctacaa acagtcgatc
     2821 gcgcagcgcg cgcggaccat ggcgctgtac aaccccatcc cggtcaagca gaactgcttc
     2881 accgtcaacc gctcgctctt cgtcttcagc gaggacaacg tcgtccgcaa atacgccaag
     2941 cgcatcaccg agtggcctcc gttcgagtac atgatcctgg ccaccatcat cgccaactgc
     3001 atcgtgctgg ccctggagca gcacctccct gacggggaca agacgcctat gtccgagcgc
     3061 ctggatgaca ccgagcccta cttcattggg atcttctgct tcgaggcggg gatcaagatc
     3121 ctggccctgg gctttgttct gcacaagggc tcctatctgc ggaacggctg gaacgtcatg
     3181 gacttcgtgg tagtcctcac ggggattctt gccacagccg gaaccgactt cgacctgcga
     3241 acgctgaggg ctgtgcgtgt gctaaggccc ctgaagctgg tgtctgggat tccgagcttg
     3301 caggtggtgc tcaagtccat catgaaggcc atggtcccgc tgctgcagat cgggctgctg
     3361 ctcttcttcg ccatcctcat gtttgccatc atcggcctcg agttctacat gggcaagttc
     3421 cacaaggcct gcttccccaa cagcacagac ccagatcccg tgggtgactt cccctgtggc
     3481 aaggaggccc cggcccggct ctgtgaggga gacacggagt gccgggagta ctgggccgga
     3541 cccaactttg gtatcaccaa ctttgacaac atcctgtttg ccatcttgac cgtgttccag
     3601 tgcatcacca tggagggctg gactgacatc ctctacaata caaacgatgc agccggcaac
     3661 acgtggaact ggctctactt catccctctc atcatcatcg gctccttctt catgctcaac
     3721 ctggtgctgg gtgtgctctc aggagagttt gctaaggagc gcgagagagt ggagaaccgc
     3781 cgcgccttcc tgaagctccg gcggcagcag cagattgagc gggagctcaa cgggtacctg
     3841 gaggcgatct tcaaggctga ggaagtcatg ctggctgagg aggacaggaa cgcagaggag
     3901 aagtcccctc tggatgcagt gttgaagaga gcagctgcca agaagagcag aagcgacctg
     3961 atccaggccg aggaggggga gggccggctc acaggcctct gtgctcccgg ttccccgttc
     4021 gcccgtgcca gcctgaaaag cgggaagacg gagagctcgt cgtacttccg gaggaaggag
     4081 aagatgttcc gcttcttcat ccggcgcatg gtgaaggcac agagcttcta ctggacggtg
     4141 ctgtgtgtgg tggcactgaa cacactgtgc gtggccatgg tgcactacaa ccagccgcag
     4201 cggctcacca cggcattgta ctttgcagag tttgttttcc tgggtctctt cctcacagag
     4261 atgtccctga agatgtatgg cctggggccc aggagctact tccggtcctc cttcaactgt
     4321 tttgactttg gggtcatcgt gggcagtatc tttgaagtgg tctgggctgc tgtcaagcca
     4381 gggacctcct tcgggatcag tgttctgcgg gctctgcgct tactgagaat cttcaaggtc
     4441 accaaatact ggaactccct acggaacctg gtggtgtctc tgctcaactc catgaagtcc
     4501 atcatcagcc tcctcttcct gctcttcctg ttcattgttg tctttgctct gctggggatg
     4561 cagctgtttg gaggccagtt caactttaaa gacgagaccc caacgaccaa ctttgacacc
     4621 ttccctgctg ctatcctcac cgtcttccag atcctgaccg gagaggactg gaatgcggtc
     4681 atgtaccacg ggatcgagtc gcaaggtggc gtcagcagag gaatgttctc ctccttctac
     4741 ttcattgtcc tcactctgtt tgggaactac accctgctga atgtcttcct ggccattgct
     4801 gtggacaacc ttgccaatgc tcaggagctg accaaggatg aagaggagat ggaagaagca
     4861 gccaatcaga aactcgcctt gcagaaggcc aaggaagtgg ctgaagtcag ccccatgtct
     4921 gctgcgaaca tctccattgc cgccaggcag cagaactcgg ccaaagcgcg ctcggtgtgg
     4981 gagcagcgag ccagccagct gcggctgcag aacctgaggg ccagctgcga ggcgctgtac
     5041 agcgagatgg accccgagga gaggctgcgc tacgccaccg cgcgccacct gcggcccgac
     5101 gtgaagacgc acctggaccg gccgctggtg gttgagcccg gccgcgacgc tccgcggggc
     5161 cccccggggg ggaagtcccg gccggatggc tcggaggccc ccgagggtgc ggatcccccg
     5221 cgcaggcatc accggcaccg agacaaggac aaggctcccg ccacggtccc ctcggcgggg
     5281 gaacaggaca gagccgaagc tctgagggca gagggcgggg agctggggcc ccgcgaggag
     5341 cgggggcggc cacgccgcag ccgcagcaag gaggccccgg gcgccccgga ggtgcgcagc
     5401 gatcgcggcc gtggcccgtg tcctgaaggt ggtcggcgcc accaccggcg cgggtcaccg
     5461 gaggaggcag ctgagaggga gccccgccgc caccgcgcgc accggcacgg gcccgacccg
     5521 ggcaaggagg gcccagcgtc cgggaccagg ggcgagcggc gcgcacgaca ccgcacgggc
     5581 ccccgggcgt gcccccggga ggctgagagc agcgaggagc ctgcgcggcg gcaccgggct
     5641 cggcacaagg cgccgcccac ccaggagacc gcggagaagg acaaggaggc tgccgagaag
     5701 gggggcgagg ccacggaggc cgagaaggac aaggaggctc ggaaccacca gcccaaggag
     5761 ctgccctgtg acctggaggc catcggcatg ctgggagtgg gcgctgtgca tacgctgccc
     5821 agcacctgtc tgcagaaggt ggaggagcag ccagaggatg cggacaacca gcggaacgtc
     5881 acccgcatgg gtagtcagcc cccggacacc agcaccactg tgcacattcc tgtgacgctg
     5941 accggccccc ccggggagac cacggtagtg cccagtggaa acgtggacct ggaaagtcaa
     6001 gcagagggga agaaggaggt ggaaaccagc gatgtgatga ggagcggccc tcggcccatt
     6061 gtcccgtaca gttccatgtt ctgtttaagc ccaaccaacc tgctgcgccg ctgctgccac
     6121 tacattgtga ccatgcgata cttcgagatg gtcatcctcg tggtcatcgc cctgagcagc
     6181 atcgccctgg ctgccgagga ccccgtgaga acagactctc ccaggaataa tgctctgaag
     6241 tacatggact acatcttcac aggcgtcttc accttcgaga tggtgataaa gatgatcgat
     6301 ttgggactgc tgctacaccc tggagcctat ttccgggatc tgtggaacat tctggacttc
     6361 attgtggtca gtggggccct ggtggcattt gctttctcag gatccaaagg gaaagacatc
     6421 agcaccatca aatccctgag agtcctgcgt gtgctgcggc cactcaagac catcaagcgg
     6481 ctgcccaagc tcaaggccgt gtttgactgt gtggtgaact cactgaaaaa cgtcctcaac
     6541 atcctgatcg tctacatgct cttcatgttc atctttgccg tcattgccgt gcagctcttc
     6601 aaagggaagt tcttctactg cacggacgaa tccaaggagc tggagcgtga ctgcaggggt
     6661 cagtatttgg attatgagaa ggaggaagtg gaagctcaac cgaggcagtg gaagaaatat
     6721 gacttccact atgacaacgt gctctgggct ctgctaacgc tcttcacggt gtccacaggg
     6781 gaagggtggc ccatggtgct gaaacactct gtggatgcca cctacgagga gcagggcccg
     6841 agccccgggt accgcatgga gctctccatc ttctacgtgg tctactttgt ggtctttccc
     6901 ttcttctttg tcaacatctt cgtggccttg atcatcatca ccttccagga gcagggggac
     6961 aaggtgatgt ccgagtgcag cctggagaag aacgagaggg cttgcatcga ctttgccatc
     7021 agcgccagac ccctgacgcg gtacatgccc cagaacaagc agtcgttcca gtataagacg
     7081 tggacgttcg tggtgtcccc accttttgag tacttcatca tggctatgat cgccctcaac
     7141 actgtggtgc tgatgatgaa gttctacgat gcgccgtacg agtatgagct aatgctgaaa
     7201 tgcctgaaca tcgtgttcac gtccatgttc tccatggagt gtgtgctgaa gatcatcgcc
     7261 ttcggggtgc tgaactattt cagagatgcc tggaatgtct ttgactttgt cacggtgttg
     7321 ggaagtatta ctgatatttt ggtaacagag attgcgaaca acttcatcaa cctcagcttc
     7381 ctgcgcctgt tccgggccgc gcggctcatc aagctgctgc gccagggcta caccatccgc
     7441 atcctgcttt ggaccttcgt gcagtccttc aaggcgctgc cctacgtgtg tctcctcatc
     7501 gccatgctct tcttcatcta cgccatcatt ggcatgcagg tttttgggaa catcgccctg
     7561 gacgatgaca ccagcatcaa caggcacaat aacttccgga cgtttctgca ggccttgatg
     7621 ttgctgttca ggagcgccac gggggaggcc tggcacgaga taatgctgtc gtgcctcagc
     7681 agccgggcct gcgacgagca ctccaatgcc agcgagtgcg gcagcgactt tgcctacttt
     7741 tacttcgtct ccttcatctt cctctgctcc tttctgatgt tgaacctctt tgtggctgtg
     7801 atcatggaca attttgagta ccttacacgg gactcttcca tcttagggcc tcaccacctg
     7861 gatgagttca tccgggtctg ggctgaatac gacccggccg cgtgtgggcg catcagttac
     7921 agtgacatgt ttgagatgct gaagcacatg tccccacccc tggggctggg gaagaagtgc
     7981 cctgctcgag tcgcgtacaa gcgcctggtc cgcatgaaca tgcccatctc cagtgaggac
     8041 atgacggtgc acttcacatc cacgctgatg gctctcattc ggacagcgct ggacatcaag
     8101 ctggcaccag ctgggaccaa gcagcaccag tgcgacgcag agctgaggaa ggagatttcc
     8161 tgtgtgtggg ccaatctgcc ccagaagacc ctggacttac tggtaccacc ccataagcct
     8221 gacgagatga cggtgggcaa agtctatgca gctctgatga tcttcgactt ctacaaacag
     8281 aacaaaacca gcagagacca gactcagcaa gcccccggag gcctgtccca gctgggcccc
     8341 gtgtccctgt tccacccgct gaaggccacg ctggagcaga ctcagccggc gctgcgtgga
     8401 gcccgggctt tcctgcggca gaagagttct gcctccctga gcaacggcgg ggccgtacaa
     8461 acccaggaga gcggcatcaa ggaatcggtt tcttggggca ctcagaggac ccaggatgtg
     8521 ctttgcgagg ccagggcacc cctggagcgg ggccactcag ctgagatccc agtcgggcag
     8581 cctgggacac tggctgtaga tgtccagatg cagaacatga ctctgagtgg ccctgacgcc
     8641 gagccgcagc cagggctgga gagccagggc cgagccgcct ccatgccccg cctggccgca
     8701 gaaacacagc ccgcgcctga cgccagcccc atgaagcgct ccatctccac gctggccccg
     8761 cgcccccaca cggcccggct cggcagcacc gcgctggacc gcccagcccc cagccaggcc
     8821 ccgcaccatc accaccaccg ctgccaccgc cgcagggaca ggaagcagcg gtccctggag
     8881 aagggtccca gcctgtctgc tgacacagat ggggcccccg acagcacggt ggggccagga
     8941 ctgcccacgg gggagggccc ccccggctgc aggcgggagc gggagcgcag gcaggagcgg
     9001 ggccggtccc aggagcggag gcagccctcg tcctcctcct cggagaagca tcgcttctac
     9061 tcctgtgacc gcttcggggg ccgcgaaccc ccacagccca agccctccct cagtagtcac
     9121 cccacgtcgc cgacggccgg gcaggagccg ggaccccacc cgcaggggag tggttcggtt
     9181 cacgggagcc ccctgctgtc cacatctggt gctagcaccc cgggccgggg gcggaggcag
     9241 ctcccccaga cccccttgac cccccgcccc agcgtcacct acaagacggc caactcctcg
     9301 cctgtccact tcgccggggc accgagcggc ctccccgcct tctcccccgg ccggctcagc
     9361 cggggcctct cagaacacaa cgccctgctg cagagggacc cgctcagccg gcccctggcg
     9421 cccggctcgc gcatcggctc cgacccctac ctggggcagc ggctggacag cgaggcgccc
     9481 gcccgtgccc tgcccgagga cgcgcccgcc tttgaggaga ccgcggccag caactcgggc
     9541 cgctcctccc ggacctcgta cgtgtcctca ctgacctcgc agcccccgcc gctgcgccgc
     9601 gtgcccaacg gctaccactg cactctgggg ctgggcgggg gcggccgggc gcggcgcggc
     9661 tgccaccacc ccgaccgcga ccgccggtgc tagactagtt ctagcaagct tggccgccat
     9721 ggcccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9781 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9841 tatcttatca tgtctggatc gggaattaat tcggcgcagc accatggcct gaaataacct
     9901 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
     9961 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    10021 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
    10081 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
    10141 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    10201 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    10261 tttttggagg cctaggcttt tgcaaaaagc tgttaacagc ttggcactgg ccgtcgtttt
    10321 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    10381 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    10441 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
    10501 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
    10561 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    10621 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    10681 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    10741 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    10801 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    10861 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    10921 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    10981 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
    11041 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
    11101 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
    11161 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
    11221 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
    11281 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    11341 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    11401 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    11461 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    11521 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    11581 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    11641 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    11701 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    11761 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    11821 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    11881 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    11941 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    12001 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    12061 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    12121 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    12181 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    12241 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    12301 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    12361 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
