LOCUS       sequence-412225-       12534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with a mutation (W391A) 
            in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that 
            prevents Beta subunit binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12534)
  AUTHORS   Leroy J, Richards MW, Butcher AJ, Nieto-Rostro M, Pratt WS, Davies 
            A, Dolphin AC
  TITLE     Interaction via a key tryptophan in the I-II linker of N-type 
            calcium channels is required for beta1 but not for palmitoylated 
            beta2, implicating an additional binding site in the regulation of 
            channel voltage-dependent properties.
  JOURNAL   J Neurosci. 2005 Jul 27;25(30):6984-96.
  PUBMED    16049174
REFERENCE   2  (bases 1 to 12534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2005-07-27"; volume: "25(30)"; pages: "6984-96"
COMMENT     Sequence Label: Cav2.2 W391A pcDNA3
FEATURES             Location/Qualifiers
     source          1..12534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1800..1819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1991..2370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2371..2574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2525..2545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2619..2638
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2619..2637
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9843..9861)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9844..9861)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9881..9898)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9887..10111
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10157..10585
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10244..10263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10454..10475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10594..10614)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10599..10928
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10779..10914
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10841..10860
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10995..11789
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11049..11068)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11659..11678
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11963..12084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12000..12019)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12054..12073
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12133..12149)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12133..12149)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12146..12168)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12157..12173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12181..12187,12188..12205,12206..12211))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12226..12247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12364..12381)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1801 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1861 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1921 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1981 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
     2581 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
     2641 acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcc
     2701 tcgagggtac cgggcccccc ctcgaggtcg aagcttgatc atggtccgct tcggggacga
     2761 gctgggcggc cgctatgggg gcgcgggcgg cgcggagcgg gcccggggcg gcggggccgg
     2821 cggggctggc ggcccgggcc ccggggggct gccgccgggc cagcgggtcc tctacaaaca
     2881 gtcgatcgcg cagcgcgcgc ggaccatggc gctgtacaac cccatcccgg tcaagcagaa
     2941 ctgcttcacc gtcaaccgct cgctcttcgt cttcagcgag gacaacgtcg tccgcaaata
     3001 cgccaagcgc atcaccgagt ggcctccgtt cgagtacatg atcctggcca ccatcatcgc
     3061 caactgcatc gtgctggccc tggagcagca cctccctgac ggggacaaga cgcctatgtc
     3121 cgagcgcctg gatgacaccg agccctactt cattgggatc ttctgcttcg aggcggggat
     3181 caagatcctg gccctgggct ttgttctgca caagggctcc tatctgcgga acggctggaa
     3241 cgtcatggac ttcgtggtag tcctcacggg gattcttgcc acagccggaa ccgacttcga
     3301 cctgcgaacg ctgagggctg tgcgtgtgct aaggcccctg aagctggtgt ctgggattcc
     3361 gagcttgcag gtggtgctca agtccatcat gaaggccatg gtcccgctgc tgcagatcgg
     3421 gctgctgctc ttcttcgcca tcctcatgtt tgccatcatc ggcctcgagt tctacatggg
     3481 caagttccac aaggcctgct tccccaacag cacagaccca gatcccgtgg gtgacttccc
     3541 ctgtggcaag gaggccccgg cccggctctg tgagggagac acggagtgcc gggagtactg
     3601 ggccggaccc aactttggta tcaccaactt tgacaacatc ctgtttgcca tcttgaccgt
     3661 gttccagtgc atcaccatgg agggctggac tgacatcctc tacaatacaa acgatgcagc
     3721 cggcaacacg tggaactggc tctacttcat ccctctcatc atcatcggct ccttcttcat
     3781 gctcaacctg gtgctgggtg tgctctcagg agagtttgct aaggagcgcg agagagtgga
     3841 gaaccgccgc gccttcctga agctccggcg gcagcagcag attgagcggg agctcaacgg
     3901 gtacctggag gcgatcttca aggctgagga agtcatgctg gctgaggagg acaggaacgc
     3961 agaggagaag tcccctctgg atgcagtgtt gaagagagca gctgccaaga agagcagaag
     4021 cgacctgatc caggccgagg agggggaggg ccggctcaca ggcctctgtg ctcccggttc
     4081 cccgttcgcc cgtgccagcc tgaaaagcgg gaagacggag agctcgtcgt acttccggag
     4141 gaaggagaag atgttccgct tcttcatccg gcgcatggtg aaggcacaga gcttctactg
     4201 gacggtgctg tgtgtggtgg cactgaacac actgtgcgtg gccatggtgc actacaacca
     4261 gccgcagcgg ctcaccacgg cattgtactt tgcagagttt gttttcctgg gtctcttcct
     4321 cacagagatg tccctgaaga tgtatggcct ggggcccagg agctacttcc ggtcctcctt
     4381 caactgtttt gactttgggg tcatcgtggg cagtatcttt gaagtggtct gggctgctgt
     4441 caagccaggg acctccttcg ggatcagtgt tctgcgggct ctgcgcttac tgagaatctt
     4501 caaggtcacc aaatactgga actccctacg gaacctggtg gtgtctctgc tcaactccat
     4561 gaagtccatc atcagcctcc tcttcctgct cttcctgttc attgttgtct ttgctctgct
     4621 ggggatgcag ctgtttggag gccagttcaa ctttaaagac gagaccccaa cgaccaactt
     4681 tgacaccttc cctgctgcta tcctcaccgt cttccagatc ctgaccggag aggactggaa
     4741 tgcggtcatg taccacggga tcgagtcgca aggtggcgtc agcagaggaa tgttctcctc
     4801 cttctacttc attgtcctca ctctgtttgg gaactacacc ctgctgaatg tcttcctggc
     4861 cattgctgtg gacaaccttg ccaatgctca ggagctgacc aaggatgaag aggagatgga
     4921 agaagcagcc aatcagaaac tcgccttgca gaaggccaag gaagtggctg aagtcagccc
     4981 catgtctgct gcgaacatct ccattgccgc caggcagcag aactcggcca aagcgcgctc
     5041 ggtgtgggag cagcgagcca gccagctgcg gctgcagaac ctgagggcca gctgcgaggc
     5101 gctgtacagc gagatggacc ccgaggagag gctgcgctac gccaccgcgc gccacctgcg
     5161 gcccgacgtg aagacgcacc tggaccggcc gctggtggtt gagcccggcc gcgacgctcc
     5221 gcggggcccc ccggggggga agtcccggcc ggatggctcg gaggcccccg agggtgcgga
     5281 tcccccgcgc aggcatcacc ggcaccgaga caaggacaag gctcccgcca cggtcccctc
     5341 ggcgggggaa caggacagag ccgaagctct gagggcagag ggcggggagc tggggccccg
     5401 cgaggagcgg gggcggccac gccgcagccg cagcaaggag gccccgggcg ccccggaggt
     5461 gcgcagcgat cgcggccgtg gcccgtgtcc tgaaggtggt cggcgccacc accggcgcgg
     5521 gtcaccggag gaggcagctg agagggagcc ccgccgccac cgcgcgcacc ggcacgggcc
     5581 cgacccgggc aaggagggcc cagcgtccgg gaccaggggc gagcggcgcg cacgacaccg
     5641 cacgggcccc cgggcgtgcc cccgggaggc tgagagcagc gaggagcctg cgcggcggca
     5701 ccgggctcgg cacaaggcgc cgcccaccca ggagaccgcg gagaaggaca aggaggctgc
     5761 cgagaagggg ggcgaggcca cggaggccga gaaggacaag gaggctcgga accaccagcc
     5821 caaggagctg ccctgtgacc tggaggccat cggcatgctg ggagtgggcg ctgtgcatac
     5881 gctgcccagc acctgtctgc agaaggtgga ggagcagcca gaggatgcgg acaaccagcg
     5941 gaacgtcacc cgcatgggta gtcagccccc ggacaccagc accactgtgc acattcctgt
     6001 gacgctgacc ggcccccccg gggagaccac ggtagtgccc agtggaaacg tggacctgga
     6061 aagtcaagca gaggggaaga aggaggtgga aaccagcgat gtgatgagga gcggccctcg
     6121 gcccattgtc ccgtacagtt ccatgttctg tttaagccca accaacctgc tgcgccgctg
     6181 ctgccactac attgtgacca tgcgatactt cgagatggtc atcctcgtgg tcatcgccct
     6241 gagcagcatc gccctggctg ccgaggaccc cgtgagaaca gactctccca ggaataatgc
     6301 tctgaagtac atggactaca tcttcacagg cgtcttcacc ttcgagatgg tgataaagat
     6361 gatcgatttg ggactgctgc tacaccctgg agcctatttc cgggatctgt ggaacattct
     6421 ggacttcatt gtggtcagtg gggccctggt ggcatttgct ttctcaggat ccaaagggaa
     6481 agacatcagc accatcaaat ccctgagagt cctgcgtgtg ctgcggccac tcaagaccat
     6541 caagcggctg cccaagctca aggccgtgtt tgactgtgtg gtgaactcac tgaaaaacgt
     6601 cctcaacatc ctgatcgtct acatgctctt catgttcatc tttgccgtca ttgccgtgca
     6661 gctcttcaaa gggaagttct tctactgcac ggacgaatcc aaggagctgg agcgtgactg
     6721 caggggtcag tatttggatt atgagaagga ggaagtggaa gctcaaccga ggcagtggaa
     6781 gaaatatgac ttccactatg acaacgtgct ctgggctctg ctaacgctct tcacggtgtc
     6841 cacaggggaa gggtggccca tggtgctgaa acactctgtg gatgccacct acgaggagca
     6901 gggcccgagc cccgggtacc gcatggagct ctccatcttc tacgtggtct actttgtggt
     6961 ctttcccttc ttctttgtca acatcttcgt ggccttgatc atcatcacct tccaggagca
     7021 gggggacaag gtgatgtccg agtgcagcct ggagaagaac gagagggctt gcatcgactt
     7081 tgccatcagc gccagacccc tgacgcggta catgccccag aacaagcagt cgttccagta
     7141 taagacgtgg acgttcgtgg tgtccccacc ttttgagtac ttcatcatgg ctatgatcgc
     7201 cctcaacact gtggtgctga tgatgaagtt ctacgatgcg ccgtacgagt atgagctaat
     7261 gctgaaatgc ctgaacatcg tgttcacgtc catgttctcc atggagtgtg tgctgaagat
     7321 catcgccttc ggggtgctga actatttcag agatgcctgg aatgtctttg actttgtcac
     7381 ggtgttggga agtattactg atattttggt aacagagatt gcgaacaact tcatcaacct
     7441 cagcttcctg cgcctgttcc gggccgcgcg gctcatcaag ctgctgcgcc agggctacac
     7501 catccgcatc ctgctttgga ccttcgtgca gtccttcaag gcgctgccct acgtgtgtct
     7561 cctcatcgcc atgctcttct tcatctacgc catcattggc atgcaggttt ttgggaacat
     7621 cgccctggac gatgacacca gcatcaacag gcacaataac ttccggacgt ttctgcaggc
     7681 cttgatgttg ctgttcagga gcgccacggg ggaggcctgg cacgagataa tgctgtcgtg
     7741 cctcagcagc cgggcctgcg acgagcactc caatgccagc gagtgcggca gcgactttgc
     7801 ctacttttac ttcgtctcct tcatcttcct ctgctccttt ctgatgttga acctctttgt
     7861 ggctgtgatc atggacaatt ttgagtacct tacacgggac tcttccatct tagggcctca
     7921 ccacctggat gagttcatcc gggtctgggc tgaatacgac ccggccgcgt gtgggcgcat
     7981 cagttacagt gacatgtttg agatgctgaa gcacatgtcc ccacccctgg ggctggggaa
     8041 gaagtgccct gctcgagtcg cgtacaagcg cctggtccgc atgaacatgc ccatctccag
     8101 tgaggacatg acggtgcact tcacatccac gctgatggct ctcattcgga cagcgctgga
     8161 catcaagctg gcaccagctg ggaccaagca gcaccagtgc gacgcagagc tgaggaagga
     8221 gatttcctgt gtgtgggcca atctgcccca gaagaccctg gacttactgg taccacccca
     8281 taagcctgac gagatgacgg tgggcaaagt ctatgcagct ctgatgatct tcgacttcta
     8341 caaacagaac aaaaccagca gagaccagac tcagcaagcc cccggaggcc tgtcccagct
     8401 gggccccgtg tccctgttcc acccgctgaa ggccacgctg gagcagactc agccggcgct
     8461 gcgtggagcc cgggctttcc tgcggcagaa gagttctgcc tccctgagca acggcggggc
     8521 cgtacaaacc caggagagcg gcatcaagga atcggtttct tggggcactc agaggaccca
     8581 ggatgtgctt tgcgaggcca gggcacccct ggagcggggc cactcagctg agatcccagt
     8641 cgggcagcct gggacactgg ctgtagatgt ccagatgcag aacatgactc tgagtggccc
     8701 tgacgccgag ccgcagccag ggctggagag ccagggccga gccgcctcca tgccccgcct
     8761 ggccgcagaa acacagcccg cgcctgacgc cagccccatg aagcgctcca tctccacgct
     8821 ggccccgcgc ccccacacgg cccggctcgg cagcaccgcg ctggaccgcc cagcccccag
     8881 ccaggccccg caccatcacc accaccgctg ccaccgccgc agggacagga agcagcggtc
     8941 cctggagaag ggtcccagcc tgtctgctga cacagatggg gcccccgaca gcacggtggg
     9001 gccaggactg cccacggggg agggcccccc cggctgcagg cgggagcggg agcgcaggca
     9061 ggagcggggc cggtcccagg agcggaggca gccctcgtcc tcctcctcgg agaagcatcg
     9121 cttctactcc tgtgaccgct tcgggggccg cgaaccccca cagcccaagc cctccctcag
     9181 tagtcacccc acgtcgccga cggccgggca ggagccggga ccccacccgc aggggagtgg
     9241 ttcggttcac gggagccccc tgctgtccac atctggtgct agcaccccgg gccgggggcg
     9301 gaggcagctc ccccagaccc ccttgacccc ccgccccagc gtcacctaca agacggccaa
     9361 ctcctcgcct gtccacttcg ccggggcacc gagcggcctc cccgccttct cccccggccg
     9421 gctcagccgg ggcctctcag aacacaacgc cctgctgcag agggacccgc tcagccggcc
     9481 cctggcgccc ggctcgcgca tcggctccga cccctacctg gggcagcggc tggacagcga
     9541 ggcgcccgcc cgtgccctgc ccgaggacgc gcccgccttt gaggagaccg cggccagcaa
     9601 ctcgggccgc tcctcccgga cctcgtacgt gtcctcactg acctcgcagc ccccgccgct
     9661 gcgccgcgtg cccaacggct accactgcac tctggggctg ggcgggggcg gccgggcgcg
     9721 gcgcggctgc caccaccccg accgcgaccg ccggtgctag actagttcta gcaagcttcg
     9781 acgaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccct
     9841 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
     9901 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     9961 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
    10021 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
    10081 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
    10141 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    10201 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    10261 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    10321 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    10381 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    10441 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    10501 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    10561 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
    10621 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    10681 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    10741 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
    10801 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    10861 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    10921 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
    10981 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
    11041 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
    11101 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
    11161 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
    11221 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
    11281 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
    11341 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
    11401 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
    11461 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
    11521 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
    11581 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
    11641 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
    11701 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
    11761 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
    11821 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
    11881 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    11941 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
    12001 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    12061 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
    12121 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    12181 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    12241 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    12301 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    12361 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    12421 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    12481 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtt
//
