LOCUS       sequence-412237-       15379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent norepinephrine (NE) 
            sensor GRAB_NE2m pan-neuronally in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15379)
  AUTHORS   Feng J, Dong H, Lischinsky JE, Zhou J, Deng F, Zhuang C, Miao X, 
            Wang H, Li G, Cai R, Xie H, Cui G, Lin D, Li Y
  TITLE     Monitoring norepinephrine release in vivo using next-generation 
            GRAB(NE) sensors.
  JOURNAL   Neuron. 2024 Jun 19;112(12):1930-1942.e6. doi: 
            10.1016/j.neuron.2024.03.001. Epub 2024 Mar 27.
  PUBMED    38547869
REFERENCE   2  (bases 1 to 15379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2024.03.001"; journalName: "Neuron"; date: 
            "2024-06-19- 19"; volume: "112"; issue: "12"; pages: "1930-1942.e6"
COMMENT     Sequence Label: pminiTol2-HuC-NE2m
FEATURES             Location/Qualifiers
     source          1..15379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(12..116)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      143..598
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        676..780
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(781..849,850..1641)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(999..1018)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1812..2400
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2301..2320
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2554..2571
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2688..2709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2724..2729,2730..2747,2748..2754)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2762..2778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2767..2789
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2786..2802
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2786..2802
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2820..2838
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2820..2837
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(2889..2900,2901..3063)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     sig_peptide     11890..11949
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    11953..11977
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     13080..13101
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(13207..13228)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(13468..13487)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    14009..14130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14046..14065)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14100..14119
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(14137..14592)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     14718..14740
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14732..14749
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14733..14749
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14756..14775
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14756..14774
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(14787..14798,14799..14986)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     promoter        complement(15051..15348)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        join(15198..15379,1..10)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     15346..15366
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tccgcctcag gactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
       61 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      121 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      181 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      241 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      301 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
      361 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
      421 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
      481 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
      541 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
      601 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc aggtggcact
      661 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      721 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      781 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      841 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      901 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      961 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1021 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1081 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1141 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1201 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1261 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1321 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1381 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1441 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1501 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1561 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1621 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1681 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1741 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1801 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1861 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1921 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1981 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2041 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2101 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2161 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2221 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2281 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2341 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2401 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2461 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2521 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2581 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     2641 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     2701 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     2761 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcta
     2821 tttaggtgac actatagaat actcaagcta tgcatccaac gcgttgggag ctctcccata
     2881 tggtcgagca gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact
     2941 tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa
     3001 agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa
     3061 gaaagaaaac tagagattct tgtttaagct tggatccgta ccgggccccc cctcgaaatt
     3121 cactaatttg aatttaaatg cattatcttt ctattcctaa agaccttggg tgaccaaaat
     3181 cttattttaa taaataaaac tgtttattaa aacttttttg tttcaaagaa ccatatgtat
     3241 agtgaaattt ataaaaatat caatttttaa aaagctggtg tactcattta tgttatgaac
     3301 tctaaaacca tatactgact gcaagtgatg atgtatagag tgatgtttac gagtaaacat
     3361 atttagttgt atacatccta ctgagcacat tttgatgtat gaaataacat tacaagcttt
     3421 atccaaatta agccatttta aaacactgcc aattgaaaat acaaatcctg gaaaaaatcg
     3481 tctttagcgc agtcatttga gccatcctaa tccgttacct cagaccataa taagaaggga
     3541 taacactagc tgtagcaatg gaacacatct gtttcacaca atcatatctc ctgcgccggt
     3601 gctaagcaga ttcagcgtga tcataacatg ctttccactc ataaatgtaa atttacaatt
     3661 tgcacatgta aaacagacac ttttgagata ttggataaaa aaacaagagt atattgctta
     3721 gtttcatcca ccagtcatcc ccacagcgtt tggaaggcca taaaaagtgt ctaaaatcaa
     3781 tgatcattga aagagcacaa gagagactct tacgctgtaa tgccactggg gacaaaagtg
     3841 acagtctctt aatgggctct tctggagggg ctcctgaaca ttaaaaatta tcagcgaaat
     3901 taccgaaaga gcttcaagca actggcatgc ttgatcctct gcgtcggggc ggtgaatagg
     3961 tgcttcagat gccctcttac ccacgggctg gattcagctg ccccgctacc agcggagacc
     4021 ccctaatgag cctctgcaat taagtttatt catgttaagt gtgaacgggg tgcgtgcgga
     4081 actgtgggca gctaacagac ctgggttctt tgtgccacaa gtgctgcctt tattcggctc
     4141 acaaagcaga aaacaacacc cgcacctata atggcgccct cggctgggtc taagaaacgt
     4201 ggcgagttga cagagcagag tgggcggggt taagacagac tgacagcggg acccatctcc
     4261 atcctcttat taacgcttaa cgagtgcctt cctcatgcaa tattcatcgc cactaatatc
     4321 atccaagctc tgagctgagc tggccactta tgtaaggcaa ttatgtaaaa tatcagacag
     4381 ggcccacact cagaatctga ctggggtaga gacgcgggac gagaaccgag agcaagaact
     4441 gaaagtgaaa gtgaccacta aagggaggag aggacagagg ggcaggatgt gtcaagatta
     4501 ccagagaaca cttggccaga aatgcgcaac cattggagct ctccggatta cccaaaggtt
     4561 aacgagtttg aacgcctctg cccactcgcc catctctgat ggtttcccaa gaactcctca
     4621 agcaaaatat atataattgt gtgtattatg cacagacacg agaaaatgct gtttttctga
     4681 tctgcattac agcacatttg cccgccaacg acaataccac ccactcggta cctcgctgac
     4741 tcctgatgcc tgatacctgc gcggtgactg tctacaatct gcataatcaa gagaagttgt
     4801 gttgaagacg agcgccacac aaccgtttcc acaaggtcac ccaaggccgg tgcagatgta
     4861 ggtgaggtct ccataaacag actgaaataa acacatcctc cgctgggaac aacaaccccc
     4921 tcacgcctca tgcatttcca taagcctaca tgcatctctt ccaacttatg gagactcgca
     4981 cctaccaaca tccgcacaac aaagatatac agagcgcgct ccctcaggtc aaggccctgt
     5041 gggggtctgt gcagaaatag gtcatttgtc acacatcaag tcctggggca ggagatgcat
     5101 tatagatgag accaaacagc ctgtctcggt gagctctacc cactccctga gactagaaat
     5161 gggggaaggg agcttgagat aacaaccgct gcaatcactg tgtcgatgtt taatatcagc
     5221 accaaccggg aacaataagg agatgggtgc attcatgttc acatcttacc agtcaagtat
     5281 catcgaaccg gcttgataac cacacctcgt gtaatagctg agcagatagt tgtcatttta
     5341 aagcgttggc ctttgtcgat tatgtaatgc gcacattcaa cacatggtaa tatagaaacg
     5401 gttatgtcga ggttgttttg tccagagatg accttcacac agttacagcc gctctgcatc
     5461 cacacaaatg gaggacttaa tcgtggactg cattcttaga aatgatctac aaagacaaat
     5521 aatgtgaaat caagaaagga caaaatttaa gtaaggggat gagggagaga gagaacgagg
     5581 ggcaaggaga aagcatggct cctgtctttt tctgcaccca tctgttcgga gtgcaggtgg
     5641 agctctattc actcagctct gcatgtgtgt ttgggggggg caggaagaaa gggagggcaa
     5701 aaggaagagt ggagagatgg tgggggctgg agggatgggg ggttctcggt gatctctcct
     5761 gaaggggata atgggagagc agcgctttgc aatggctgcc atgtagtacc ctccctgcac
     5821 aattagccaa tcagcagcaa gctctgccag ccagaaggac acataaaaga agaacattgc
     5881 agcagaggca cagaaggagc ctgcgaggag ctgggaaata cacacacaac agcagaacca
     5941 caacaccctc ccctggacac accctactgg ggatcactgc ttttcttttt ttctgaacca
     6001 tcgcccacgc cacacggaga gaaatctctc tctcatcatc atcctgaaga aaaccccctt
     6061 atcctcattt tcacactgct gaggaaaacc tacaatcgca cgggctgaga tttcctggcg
     6121 aagactgtcc tttttccttt ttcttttttt ttcttttcct ttggaaactg acatttgcat
     6181 ttctccattc caagccacgg cgtaataata tctgcaatcc agcctgaaga cctgcaaatc
     6241 gaaggaccta gatcactatc atctttgtac gtcaaggatc ctggttactg tacgtataac
     6301 ctttctttct tttgctctga ccaatatgaa acactaaaat cgattcgagc agcctctcag
     6361 catcaattac agtgcgtgaa aaacattcaa atagaggcag caaatattac atgtgaaaat
     6421 acaggctggc taaatccaag ctaatttaga aatgtggtca aaacgcatac tggcacgtct
     6481 aatcgcggat tcagtaaaca agattaacga ttagcccagt gtataagtca tatgatacag
     6541 gcatgcgcga gagcatcgct acacccgagc tggcttcatt ttcggaggaa aatcaaaaca
     6601 ttgctttctc ctgccgtgcg aaccattcgt cataaaccgt aatacgcaac atacatttat
     6661 tactacatcc gttaattagc gataattagc cgttattaac aaagagcgct gaggaattcc
     6721 tctcaaatag cggaggtgcg gcggaggcag aggggcgtaa aagggcacat gcgtgcctgg
     6781 ctcaaaaaag gatgctccag actgaaactc agagacaaaa cacgatgcct cggaggctga
     6841 gagcccatcg agaggaaagg caaagaaaag ggtcgagtca ctcagagggg gaggggagat
     6901 attgtgcatc cattggtttg aattgaagca ggcagaataa aaatccttgc gattatgctt
     6961 cgtgagagat cgcgagagag gaaaaggcta aatgccacgt atgcaaatgg ataaatgtca
     7021 tctatttctt cgcgagtaga cattttgtgg acaacgagag tgtaaataag cgatatgagg
     7081 ggaatacaaa ggccttgagg gaaagcgttt tgggcgtgtc ttgtcgaaaa gaagacagcg
     7141 atggcacgcg ctcagaacca ccatcttcac gtagataatc cgcgtgaaaa caataacaaa
     7201 actgctcatt ttaacacgag caggtgtttg aaaccaccca aaaaagattg tctaaaatat
     7261 taagattaat taatgacgtt taaatgtcca ggcctgatat aaatgaagcg tgtagtaaga
     7321 gtaatcgcct ttgtttcttt tcagagatac gttttcagag attcagggaa ttatatcttc
     7381 aataaaataa tgtttgattt cattatttta aagcttttta agaagtaaaa atacacatta
     7441 ttcatttaca ttttatttta tttaaaggcc tatggtcttg aaaataataa gtggaatata
     7501 taggctatat attcgtctaa ggacagccgg tgttatttta tagtgacctc agacaaaagc
     7561 cgagacaaaa cgaggcgctt gcctttcatt ttaatcctta atagacggtg tgatgaatga
     7621 atgaatgaat gaataaatga atgaatgacg ggcccttcgg catgttgttc cgcagtcctc
     7681 cgtgagcacg agtctctacc gcagtgccaa gcacataaaa ccgttgagtc agataacctt
     7741 ctccaggcgc gtatacacaa ttcaaggaca agcacatcac aactatacac aacacaacac
     7801 aaccgcctaa acgcacgagc tcatcaatcg caatttaaag gcatgtcagt caaagaaaaa
     7861 ggcggcgtta tttaaaacca atgttcaaca atatttgtat gctgactagc ccgggcgcgc
     7921 acgctataag atcacaaggc cttgtttttt ccctttattc aaaatatcat taattattac
     7981 aggtcataaa acaaaacaat gcgaattaca atgatttaaa gactattgat ttaacaataa
     8041 tcagttaatt atagaataat attataatat tttacataca aatgaaagat gacattattt
     8101 gatgacattg taattaaaat atatgcaata tatttttacg tttttattaa ttaggctatt
     8161 actattatta taattatcat tatttttatt attattatta ttattaaata gtcttgcgaa
     8221 ataagagtga aggcagttgc aatgttcatt gttcccggag agtcccgcag cctctggcct
     8281 caacacaaag cgctactgtt tacattaata ttcatagtgc tgccatggtc tctgtagagg
     8341 aggaacgggt ggctcttttg ttcgctagtg tcaatacgtt tattcagcaa aaagcagctc
     8401 ggatttgtga atgcccaaca agcacacgcg cactccaatc aaaggacgag aggataacga
     8461 ttttaaacga ttcgcaaacg tgtttatttg taaagacgac atcaagagct taatgtccac
     8521 ttgaaaagaa ataaatatgc ccgcggcgcc aaggatattt tgaatgtagt cgttcatggt
     8581 gagccactcc cgaattcagc actctggaga gcgaccaaag aggaatagcg gaatagcaac
     8641 tacttgagcc atttctctcc gtttgatggc gttcatgatt aaaaatataa tcacgtgatt
     8701 ttttatacat atataaatat atacataaag taacgctttc cttttgagaa tacataaata
     8761 tttattcgcg tgataaatca atgatcttat atttatttgc acgaccgaaa ttagccaaat
     8821 tcaccaatta aaaaaaaaat aagccaacaa aaaagaaggg tttatccgtt caattttgac
     8881 ttgtgtcctg ttgttttaca gctgccactg tgatccctta agctgcagtg agagtgtggc
     8941 taatgccttt tgtttaagat aatcctgtaa tctgttaccg aaacggccta ttgacaagcc
     9001 ggcattcaca tttcagtcag gaggcccgtc cagacatgta catttatgaa ttacgaatga
     9061 taaaattaag atctgcatta ggcgtcagaa atgtcacgaa cacccattca tttcccacag
     9121 ccaataatgg atcatgctgg gaagcgctat cctcgcagtc tcaaaattaa tgttgacatg
     9181 ttgcgtagag ctacagatta gatcagccat atgctttatg tgtttacctc agcggatctt
     9241 cagcaagatg tgtttatttt aagaaaatgg cttcctcgct gcctgcacag gggcattaag
     9301 gaagtgacgt gagcgtcctc acagtcaaat tactttatct catgctgctt tcaggcctct
     9361 gcttatttta ttattattat tttaaattag ggggaatcac ggtggcgttg tgggtagggt
     9421 agcgcgatca ccttacagca agaaggtcgc tggttcgagc tctggctggg tcagttggca
     9481 tttctgtgtg gagtttaaat tgaattgaaa ttgaataaac taaattggcc ctagtgtatg
     9541 tgtgtgaatg taagtgtgca tgggtgtttc ccagtgttgg gtttgactgg aagagcatcc
     9601 gcttcgtaaa acatatgctg gataagttgg cggttcattt cactgtggtg accgctgatt
     9661 aataaaggga ctaagctgaa aatgaaatga attaatacat tttaaatgag cttttgctga
     9721 catgtttatg attacaaata gtgaaattgt atgttaattt acgataagtg cattgttcta
     9781 aattcatttt tatgtagcag ctggtttttc ttccatttgt gtcattcaac agaacatttt
     9841 tctaaaaaag tgaacttaaa ttttctcctc cctctgacaa ggcttgatgg taatttcagc
     9901 cgactctaat tacagactca ctaaaaggaa tcggtgcagt catttatctt tatcagcgcg
     9961 ctggtgggaa ggtaataggt tttgcttatg agttgtttgc gtgcgagtct gggtccttgt
    10021 gtaggcgatg ctgatgcact gcattaaata ttgagggaga agtcgctcaa aaatagcaaa
    10081 gtacaggctg gggcaggaac gtcaaagccc gctcagtggc cacgtttctt gtttcgtaag
    10141 tcctattttt aatagcgata attcaggctc agtagaagga actccagcac tataagcagt
    10201 ctctcgcctg tctcggcgag ttatgtattt tgggatgaag tgctcttcca cctctacctc
    10261 tgctgtgctg gcgccagtat ctgggctgta agatagtgta cgtgtcagtc tgtgtggccg
    10321 aagagcctgt gtgttgtgta actcactaca ttcgtcgctg tggatggctg acttgccatg
    10381 gggaggttta cttcagttca cgaatcacat tcgccacgat cttgtcactt tgtgtcaaga
    10441 ctcgctctct gtgtgtcaac agctctgctg tttgaggttg aaattctgaa cttgagatat
    10501 atcccaggat atattagggc tactgccaac ggaaaaagca aatggtgcca tggccatttg
    10561 attgatcaaa caaacaccct tttgcttggg tgacagtgaa atgtcagatc catgtttgta
    10621 ctgctttaat aacttgcctc tctgagcttt ctccttgtca gtgtcaattt gcctgagata
    10681 taaattccgg ttgccccgaa agcttatttt attaaaaaaa agctaacatg cactcataca
    10741 acgtgcgcac atacatgtga ggcttgctaa aagtcaagaa gcaagccaat agcaagagtc
    10801 gtcagactgc tgttacctgg gcaacctggt tagaggtaag gctcaaggca agaataaatc
    10861 attccactga agagacacag cgttacagcg agatgagagc aagaggcgat agtgaaggga
    10921 gaacaaaaga acagcctacc cacactagtg cagatctacc tgtttgagct actgattgtt
    10981 cagtttgatt agatatcaga caattctgct atctccaatt gcattgctgc atgaattcct
    11041 agaaatcatt ttaatcacca aaaactagta gggacacttg tggcgtatat gattctgcct
    11101 gttgattgtg gggatccaac agaaagatca tgaatgcatt gttaattaag tcggtgagac
    11161 accctgttcc accctagggg ccgttctggg gaaagagtgc tttcagtcag ctaaagatga
    11221 ctaatatgtg aacatatatt actacacatg ccaatttgta cattctggat agttaccagc
    11281 cctgggaaaa catcgaaaaa aataaatcaa catagataaa attggcaaat cccttctggt
    11341 acgatgcaga gtgcttgctc tgagaacttg tgcattgtga cagaaagcga ccgttaaaaa
    11401 ctgaaagtag catctacaac ctgcatacat ctgttctttg aaaatcccct gtaggccaaa
    11461 ttaagcatca cgctgtgctc cacagcaaca cggatgaacc gaaaacccag acacaaacgc
    11521 acacactttt cacataaaca aggtttagtt atgctagcta tgctttttcg tcctcccttt
    11581 gtcatcagcg gcgagtgacg taacacagtt tgaccctgga cagcgtaaag ggggggttgg
    11641 gaatgagtga aaggactgtg acatctgcag ggccaggggc tgatggagag agctgaagtg
    11701 agggaggtca gaggaggagg aagggtggga tattgtcctc ctcaggccct atccctctca
    11761 gacgcattga tttttaacct ctcagcgaga atgccaagca ttacacccct caaattatgg
    11821 ttcctcaccc aacctgttat attttccacc tgcagataat tagcaccatt gaaactcgag
    11881 gccgcaaaca tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt
    11941 tccactggtg acacaagttt gtacaaaaaa gttggcacca ccggtggctc cctgcagccg
    12001 gacgcgggca acgcgagctg gaacgggacc gaggcgccgg ggggcggcgc ccgggccacc
    12061 ccttactccc tgcaggtgac gctgacgctg gtgtgcctgg ccggcctgct catgctgctc
    12121 accgtgttcg gcaacgtgct cgtcatcatc gccgtgttca cgagccgcgc gctcaaggcg
    12181 ccccaaaacc tcttcctggt gtctctggcc tcggccgaca tcctggtggc cacgctcgtc
    12241 atccctttct cgctggccaa cgaggtcatg ggctactggt acttcggcaa ggcttggtgc
    12301 gagatctacc tggcgctcga cgtgctcttc tgcacgtcgt ccatcgtgca cctgtgcgcc
    12361 atcagcctgg accgctactg gtccatcaca caggccaaag agtacaacct gaagcgcacg
    12421 ccgcgccgca tcaaggccat catcatcacc gtgtgggtca tctcggccgt catctccttc
    12481 ccgccgctca tctccatcga gaagaagggc ggcggcggcg gcccgcagcc ggccgagccg
    12541 cgctgcgaga tcaacgacca gaagtggtac gtcatctcgt cgtgcatcgg ctccttcttc
    12601 gctccctgcc tcatcatgat cctggtctac gtgcgcatct accagatcgc caagcgtcgc
    12661 acccgcgtgc cacccagccg ccggggtccg gacgccgtcg ccgcgccgcc ggggggcacc
    12721 gagcgcaggc ccaacggtct gggccccgag cgcagcgcgg gcccgggggg cgcagaggcc
    12781 gaaccgctgc ccacccagct caacggcgcc cctggcgagc ccgcgccggc cgggccgcgc
    12841 gacaccgacg cgctggacct ggaggaggga ggaaacgtct atatcaaggc cgacaagcag
    12901 aagaacggca tcaaggcgaa cttccacatc cgccacaaca tcgaggacgg cggcgtgcag
    12961 ctcgcctacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
    13021 aaccactacc tgagcgtgca gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac
    13081 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
    13141 aagggcggta ccggagggag catggtgaga aagggcgagg agctgttcac cggggtggtg
    13201 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
    13261 ggtgagggcg atgccaccga gggcaagctg accctgaagt tcatctgcac caccggcaag
    13321 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
    13381 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
    13441 atccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
    13501 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
    13561 gacggcaaca tcctggggca caagctggag tacaacaccg gagcagcagc acgctggcgc
    13621 gggcggcaga accgcgagaa gcgcttcttc ttcgtgctgg ccgtggtcat cggagtgttc
    13681 gtggtgtgct ggttcccctt cttcttcacc tacacgctca cggccgtcgg gtgctccgtg
    13741 ccacgcacgc tcttcaaatt cttcttctgg ttcggctact gcaacagctc gttgaacccg
    13801 gtcatctaca ccatcttcaa ccacgatttc cgccgcgcct tcaagaagat cctctgtcgg
    13861 ggggacagga agcggatcgt gttgtgagcg gccgcgactc tagatcataa tcagccatac
    13921 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    13981 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    14041 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    14101 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
    14161 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
    14221 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
    14281 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
    14341 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
    14401 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
    14461 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
    14521 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
    14581 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    14641 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    14701 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
    14761 cgactcacta tagggcgaat tgggcccaga ggtgtaaagt acttgagtaa ttttacttga
    14821 ttactgtact taagtattat ttttggggat ttttacttta cttgagtaca attaaaaatc
    14881 aatactttta cttttactta attacatttt tttagaaaaa aaagtacttt ttactcctta
    14941 caattttatt tacagtcaaa aagtacttat tttttggaga tcacttcatt ctattttccc
    15001 ttgctattac caaaccaatt gaattgcgct gatgcccagt ttaatttcct ccaaaaaagc
    15061 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
    15121 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
    15181 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
    15241 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
    15301 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
    15361 cattccacag ctggttctt
//
