LOCUS       sequence-412242-        8542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the red 5-HT sensor GRAB_r5-HTmut in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Deng F, Wan J, Li G, Dong H, Xia X, Wang Y, Li X, Zhuang C, Zheng Y,
            Liu L, Yan Y, Feng J, Zhao Y, Xie H, Li Y
  TITLE     Improved green and red GRAB sensors for monitoring spatiotemporal 
            serotonin release in vivo.
  JOURNAL   Nat Methods. 2024 Apr;21(4):692-702. doi: 
            10.1038/s41592-024-02188-8. Epub 2024 Mar 5.
  PUBMED    38443508
REFERENCE   2  (bases 1 to 8542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-024-02188-8"; journalName: "Nat Methods"; date: 
            "2024-04"; volume: "21"; issue: "4"; pages: "692-702"
COMMENT     Sequence Label: pDisplay-GRAB_r5-HTmut
FEATURES             Location/Qualifiers
     source          1..8542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        380..583
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     534..554
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     628..647
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        628..646
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     727..786
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    790..814
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2193..2212
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2745..3296
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2889..2906)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3115..3134
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3308..3310,3311..3313,3314..4024)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3308..3310,3311..3313,3314..4024)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3353..3374)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3614..3633)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3961..3982
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4082..4111
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4115..4261
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4283..4300)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4289..4513
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4645..5233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4725..4744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5562..5609
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5634..5653
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5841..6635)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5841..6635)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5952..5971)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6562..6581
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6734..6753)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6832..7023
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6980..7000
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7083..7874,7875..7943))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7706..7725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7944..8048)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8075..8530
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8162..8181)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8372..8393
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        join(8542,1..379)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
       61 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      121 cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
      601 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg
      661 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttggggata
      721 tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc
      781 actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtgacaaact tgatgctaat
      841 gtgagttcca acgagggttt caggtccgtg gagaaggtcg tgctgctcac gttccttgca
      901 gtggttatcc tgatggccat cttgggcaac ctgctggtga tggtggctgt gtgcagggac
      961 aggcagctca ggaaaataaa aaccaactat ttcattgtgt ctctcgcctt tgctgacctg
     1021 ctggtttcgg tgctggtgat gccctttggt gccattgagc tggtccaaga catctgggct
     1081 tatggggaga tgttctgcct ggtccggacc tctctgcagg tctacctttg tacagcatcg
     1141 atctttcacc tgtgctgtat ttccctggac cactattacg ccatctgctg ccagcctttg
     1201 gtttatagga acaagatgac ccctctacgc atcgcattaa tgttgggagg ctgctgggtc
     1261 cttcccatgt ttatatcttt tctccccata atgcaaggct ggaacaacat cggcatagtt
     1321 gatgtgatag agaaaaggaa attcagccac aactctaact ccacgtggtg tgtcttcatg
     1381 gtcaacaagc cctatgctat cacctgctct gtggtggcct tctacatccc gtttctcctc
     1441 atggtgctgg cctattaccg aatctatgtc actgctaagg agcatgccca gctctggccc
     1501 gtgtgttccg aggttatgta ccccgaggac ggcgccctga agagcgagat cgtgaagggg
     1561 ctgaggctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
     1621 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cgtgtcccac
     1681 aacgaggact acaccatcgt ggaacagtgc gaacgcgccg aggcccgcca ctccaccggc
     1741 ggcatggacg agctgtacaa gggaggtaca ggcgggagtc tggtgagcaa gggcgaggag
     1801 cacaaccacg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccttc
     1861 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga ggcctttcag
     1921 accgctaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     1981 cctcagttca tgtacggctc caaggcctac attaagcacc cagccgacat ccccgactac
     2041 ttcaagctgt ccttccccga gggcttcagg tgggagcgcg tgatgaactt cgaggacggc
     2101 ggccgcattc acgttctgca ggactcctcc ctgcaggacg gctacttcat ctacaaggtg
     2161 aagctgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     2221 tgggagctga cggtgcacga ccagcacagc acaagtcgca tgaggacaga gaccaaggca
     2281 gccaagactt tatgtatcat catgggctgc ttctgtttct gctgggcccc cttctttgtc
     2341 accaatattg tggacccttt catagactac actgtccccg agcaggtgtg gatcgctttc
     2401 ctctggcttg gctatatcaa ttcggggttg aacccttttc tctatgcctt cttgaataag
     2461 tctttcagac gtgccttcct catcatcctc cgttgcaatt ataaggtaga gaaaaagcct
     2521 cctgtcaggc agattccaag agttgccgcc actgctttgt ctgggaggga gcttaatgtt
     2581 aacatttatc ggcataccaa tgaaccggtg atcgagaaag ccagtgacaa tgagcccggt
     2641 atagagatgc aagttgagaa tttagagtta ccagtaaatc cctccagtgt ggttagcgaa
     2701 aggattagca gtgtgtaagc tagcgcccct ctccctcccc cctaacgtta ctggccgaag
     2761 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     2821 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     2881 tctttcccct ctcgccaaag gaatgcaagg tctgttaaat gtcgtgaagg aagcagttcc
     2941 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     3001 cccacctggc gacaggtgcc tctgcggcca aagccacgtg tataagatac acctgcaaag
     3061 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     3121 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     3181 tctgatctgg ggcctcggta cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     3241 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     3301 cacaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3361 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3421 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3481 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3541 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3601 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3661 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3721 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3781 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3841 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3901 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3961 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4021 caaggatggt aaaaagaaga aaaagaagtc aaagacaaag tgtgtaatta tgtaagtcga
     4081 cgaacaaaaa ctcatctcag aagaggatct gaatgctgtg ggccaggaca cgcaggaggt
     4141 catcgtggtg ccacactcct tgccctttaa ggtggtggtg atctcagcca tcctggccct
     4201 ggtggtgctc accatcatct cccttatcat cctcatcatg ctttggcaga agaagccacg
     4261 ttaggcggcc gctcgagatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4321 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4381 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4441 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4501 gtgggctcta tggcttctga ggcggaaaga accagtggcg gtaatacggt tatccacaga
     4561 atcgggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4621 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4681 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4741 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4801 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4861 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4921 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacgcgactt
     4981 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5041 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5101 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5161 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5281 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5341 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat
     5401 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     5461 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     5521 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     5581 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     5641 cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc
     5701 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     5761 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     5821 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     5881 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     5941 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     6001 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     6061 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     6121 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     6181 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     6241 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     6301 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     6361 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     6421 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     6481 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     6541 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     6601 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     6661 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     6721 ctcagaggcc gggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     6781 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     6841 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     6901 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     6961 gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct
     7021 caggactctt cctttttcaa taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7081 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7141 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7201 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7261 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7321 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7381 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7441 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7501 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7561 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7621 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7681 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7741 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7801 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7861 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7921 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7981 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8041 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     8101 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     8161 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8221 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8281 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8341 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8401 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     8461 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     8521 cttacaattt acgcgcgcgt tg
//
