LOCUS       sequence-412247-       12295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibly expresses human Caspase 4 lacking the CARD domain, fused 
            to light-activatable Cry2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12295)
  AUTHORS   Shkarina K, Hasel de Carvalho E, Santos JC, Ramos S, Leptin M, Broz 
            P
  TITLE     Optogenetic activators of apoptosis, necroptosis, and pyroptosis.
  JOURNAL   J Cell Biol. 2022 Jun 6;221(6). pii: 213145. doi: 
            10.1083/jcb.202109038. Epub 2022 Apr 14.
  PUBMED    35420640
REFERENCE   2  (bases 1 to 12295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-06-6"; volume: "221(6). pii: 213145. doi"; 
            pages: " 10.1083/jcb.202109038. Epub 2022 Apr 14"
COMMENT     Sequence Label: pLVX-opto-hCaspase-4 dCARD
FEATURES             Location/Qualifiers
     source          1..12295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(382..393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             706..1339
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1467..1483)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1467..1483)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1480..1502)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1491..1507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1515..1521,1522..1539,1540..1545))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1560..1581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1698..1715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1869..2457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1949..1968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2628..3419,3420..3488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3251..3270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3489..3593)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    3641..3775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             3943..4576
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4623..4748
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5245..5478
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5663..5707
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5970..6085
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     polyA_signal    6129..6263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7363..8856)
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     CDS             complement(8872..9579)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9147..9166)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9416..9434
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(9585..9952)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    9710..9728
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9746..9764
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9782..9800
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9818..9836
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9854..9872
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9890..9908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9926..9944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        9969..10479
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10398..10416
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             10498..11244
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        11255..11584
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11435..11570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11593..12192
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11593..11612)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12089..12109
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(12206..12295,1..499)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12259..12279)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
       61 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
      121 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
      181 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
      241 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
      301 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
      361 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
      421 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
      481 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
      541 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
      601 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
      661 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
      721 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
      781 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
      841 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
      901 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
      961 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     1021 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     1081 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     1141 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     1201 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     1261 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     1321 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     1381 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc
     1441 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     1501 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     1561 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1621 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1681 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1741 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1801 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1861 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1921 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1981 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2041 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2101 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2161 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2221 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2281 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2341 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2401 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2461 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2521 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2581 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2641 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2701 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2761 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2821 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2881 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2941 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3001 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3061 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3121 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3181 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3241 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3301 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3361 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3421 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3481 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3541 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3601 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
     3661 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3721 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     3781 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     3841 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     3901 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     3961 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     4021 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     4081 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     4141 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     4201 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     4261 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     4321 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     4381 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     4441 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     4501 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4561 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     4621 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     4681 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     4741 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     4801 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     4861 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     4921 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     4981 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     5041 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     5101 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     5161 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     5221 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     5281 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     5341 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     5401 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     5461 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     5521 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     5581 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     5641 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     5701 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     5761 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     5821 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     5881 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     5941 attagtgaac ggatctcgac ggtatcgcct ttaaaagaaa aggggggatt ggggggtaca
     6001 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     6061 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
     6121 atcgacttaa cttgtttatt gcagcttata atggttacaa ataaggcaat agcatcacaa
     6181 atttcacaaa taaggcattt ttttcactgc attctagttt tggtttgtcc aaactcatca
     6241 atgtatctta tcatgtctgg atctcaaatc cctcggaagc tgcgcctgtc ttaggttgga
     6301 gtgatacatt tttatcactt ttacccgtct ttggattagg cagtagctct gacggccctc
     6361 ctgtcttagg ttagtgaaaa atgtcactct cttacccgtc attggctgtc cagcttagct
     6421 cgcaggggag gtggtctgga tccgccggca ccggtgtata cgggaatttc aattgccagg
     6481 aaagaggtag aaatatcttg tcatggacag tcgttctatg gtgggcattt gagctttggc
     6541 ccttggagtt tcaaatgatt gctgtacctt ccgaaatact tcctctaggt ggcagcacca
     6601 agaatatttc tggaagcatg tgatgagttg tgtgatgaag atagagccca ttgtgctgtc
     6661 tctccaggac acgttgtgtg gcgttgaaga gcagaaagca atgaagtcct tctccacgtg
     6721 ggtcttgtaa acagcatctt cctccaggtt ctcagatgac tgtgaagagg ccacttccaa
     6781 ggatgctgga gagtctctga cccacagttc cccacggttt gcacctctgc aggcctggac
     6841 aatgatgacc ttgggtttgt ccttcagact gaggcagttg cggttgttga atatctggaa
     6901 gatggtgtca taaagcagca catctggttt tttctcatca tgcacagttc cgcagattcc
     6961 ctccaggatg ccatgagaca tgagtaccaa gaatgtgctg tcagaggact tgtgctctgg
     7021 tctggtagca aatgccctca gcgctgactc catatccctg gctgtcagat tctcttctac
     7081 atctacacta tagtccagac cctcaagtag ctccttcatc cctgtgatgt caaagtcagc
     7141 tccattcctc ggaggcagat ggtcaaactc tgtattgcat atgatgagag ccaggcgtgt
     7201 gcggttgttt ctctccttta ttggatagat ctcttcagct ctttctttac atagtctcag
     7261 gaattcttca tgaggacaaa gcttgagggc atctgtagat tctcctgact caggtggtcc
     7321 agcctctcct cctcctccgg tggcgaccgg tggatcccgg gctgctgctc cgatcatgat
     7381 ctgtgctcca cgggttcttg aaatagcttt agctagtagc tcacgagctg tgtcgatgtc
     7441 tacaatgggt ttcgcatagt ttgttccgag ttccacacca gaagctttga gtacggttaa
     7501 aggagcgtcc catggatgat ggatccattc agttggcaat ctcgcaagct cgggaagcca
     7561 ttgccttatg tactcacctt ctgggtcata tttggcgcct tgtaacgcgg gattgtccaa
     7621 gcgatcaagc tcgtggccat cggggatact cccagagata tactgccagc caaggatgtc
     7681 acattccaaa tcagcatcca aaagtgtatc ccagaaatac ttcattcccc atttccatgg
     7741 aaggagaaga aacttcacag caaagcttga aacaatcact cttattctgt tatgcatcca
     7801 tccggtagcc caaagctctc tcattccggc atccaccaac ggataaccgg tcctgccttg
     7861 tctccaggcc ttgaacttat caacatcagc atcccaaggg aaaaaccgaa gatgactcaa
     7921 caacgattgc tcgtgagtaa acgggaagtt gaaacatata taccgagaat actctcttaa
     7981 accgattccc ctaagaaaaa gatctgcact ttcttctcct tcactgttct tatctcttgc
     8041 ccatataatt tgtttcatcc gggcacactg gaaaacgtgt ctgacgctta tttccccgaa
     8101 atggagatac ggagaaagta gtgaagtaga attcccaaca actttcttgc tgttctttgc
     8161 ataatctatc aactgcttct cgatgaactc atttagtaac ttatcagcat tgctccatcc
     8221 tggagaccaa gctctagtta acaacgcatt gctcggtttc tcggcctcat tctccagccc
     8281 tagttcttca atcgaacacg cccaaatcgc ttcagccgct gcagttattg gcatcaaccg
     8341 ccaaggagga ggaagcataa cggattcaat cgacatatct aagcatttct tccagtaaga
     8401 attgaaactc gtaaaaggtt tgcccttttc gcagtatatc tcccacggtt catacaatag
     8461 atctccattg tagctttgca cagagatccc acgttccacc agcttctcct ttacggtatg
     8521 gtcccgaact aacgaaacag gatcatagag gtggttaaag acgacttttg tagcaccggt
     8581 aacgcggata caatccaaga tcgctgaaat cgtgttgtgg gttttgatta aagtgaggtc
     8641 agatccaaga gccttcaagg attgagataa gtgagcaagt gattgtttca tccaccatct
     8701 tgaagctctt ccaggataaa actgtccttc ttcttcagga caccaaatga agacaggaaa
     8761 aacagatcct tcgtgagcag ctgctgctaa tgcaggatta tcctcaatcc ttaggtctct
     8821 tctaaaccaa actatagtct ttttgtccat cttcatcatt cctcctcctc ccttgtacag
     8881 ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt
     8941 gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag
     9001 ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc
     9061 cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg
     9121 ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa
     9181 gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg
     9241 ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc
     9301 ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga
     9361 gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt
     9421 caccttcagc ttggcggtct gggtgccctc gtatgggcgg ccctcgccct cgccctcgat
     9481 ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt
     9541 gatgatggcc atgttatcct cctcgccctt gctcaccata attctttacg agggtaggaa
     9601 gtggtacgga aagttggtat aagacaaaag tgttgtggaa ttgaagttta ctcaaaaaat
     9661 cagcactctt ttataggcgc cctggtttac ataagcaaag cttatacgtt ctctatcact
     9721 gatagggagt aaactggata tacgttctct atcactgata gggagtaaac tgtagatacg
     9781 ttctctatca ctgataggga gtaaactggt catacgttct ctatcactga tagggagtaa
     9841 actccttata cgttctctat cactgatagg gagtaaagtc tgcatacgtt ctctatcact
     9901 gatagggagt aaactcttca tacgttctct atcactgata gggagtaaac tcgaggtgat
     9961 aattccacgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
    10021 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
    10081 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
    10141 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
    10201 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
    10261 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
    10321 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
    10381 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
    10441 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatcatcg aattaccatg
    10501 tctagactgg acaagagcaa agtcataaac tctgctctgg aattactcaa tggagtcggt
    10561 atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg
    10621 tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac
    10681 aggcatcata cccactcctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac
    10741 aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc
    10801 ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt
    10861 cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca
    10921 ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct
    10981 accaccgatt ctatgccccc acttctgaaa caagcaattg agctgttcga ccggcaggga
    11041 gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta
    11101 aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca
    11161 gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt
    11221 gaccttgaca tgctccccgg gtaaacgcgc gaatgtgtgt cagttagggt gtggaaagtc
    11281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
    11341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    11401 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    11461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    11521 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    11581 caaaacgcga ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
    11641 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
    11701 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
    11761 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
    11821 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
    11881 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
    11941 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
    12001 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
    12061 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
    12121 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
    12181 cccggtgcct gaacgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
    12241 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg cttta
//
