LOCUS       sequence-412253-       13728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  R6K plasmid encoding a type I-F CAST but no CRISPR array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13728)
  TITLE     Distinct horizontal transfer mechanisms for type I and type V 
            CRISPR-associated transposons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pR6K-GFP-CASTIF
FEATURES             Location/Qualifiers
     source          1..13728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      108..194
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      286..313
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    complement(391..438)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(455..1114)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1029..1048
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1115..1217)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1481..1528)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1524..1542
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(2977..2988)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9950..10663)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             10670..10681
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(10761..10795)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     complement(10797..10814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     oriT            11055..11163
                     /note="incP origin of transfer"
     rep_origin      11214..11570
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(11979..12770,12771..12839))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12602..12621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12840..12944)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13190..13209
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13418..13434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13604..13620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 accataagtt gatatttttg tgaatcgagt atttcagcaa aactactgca gtaaggactc
       61 tagaagggca ccaataactg ccttaaaaaa agcagctatg ccggtcccaa ataaaatgaa
      121 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
      181 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
      241 ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga
      301 cggatggcct ttttgcgtgg ccagtgccaa gcttgcatgc agattgcagc attacacgtc
      361 ttgagcgatt gtgtaggctg gagctgcttc gaagttccta tactttctag agaataggaa
      421 cttcggaata ggaacttcat ttaaatggcg cgccttacgc cccgccctgc cactcatcgc
      481 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
      541 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
      601 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
      661 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
      721 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
      781 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
      841 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgtaatt
      901 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
      961 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1021 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     1081 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     1141 tcgacaactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1201 ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat
     1261 caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca ccactggccg
     1321 tcgacaggat ccccggaaat gacctcgagg aaaatgtcgt aaacttactg aagtttagac
     1381 catgaagagg caatatcaat ttatgggtgt gataattatc aatttatggg tgtaattatc
     1441 attttatggt tgtatcaaca acaaatgcct gaggtttcag caaaaaaccc ctcaagaccc
     1501 gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca gcgcagcagc
     1561 ctaggttatt cgaaaaagtt tttatttcta cctggtttac tcactctcgg ggttgaatgt
     1621 ctcaaatagt cctcccaacc aatatctttt ggtggcggtc gtaacatatc ttctgttacc
     1681 tcaacaccct cctcaaaagg atttttccat gagttgggtt tatcgcccga tgtcaacttc
     1741 gatgcaaaaa cgaaatcttc ttcggttatc gtctcatgct gattatcaat tgcttcgata
     1801 gatgcttgat aaataaggtt tctcaacgaa cgcatgtttc cctgagagaa tgcatacaat
     1861 tttttctgca aactttcgtt ggctaagcca gcctgttttt caaaaggtag ggctttatca
     1921 aggtattcca aaaaagtttt aaatacacct ctacctccct ggtagctaaa ggggcgcagt
     1981 tcaacctgaa tggaaaatcg cccgtgaagt tgcgagtttg cttgcagtac aacttttgag
     2041 tatggcatac caaatataac aattggacat ttcgttttgt taagtatcat ttttagccaa
     2101 ttacctactt gggtaagaac ccgatttgac ctttcttcca ccaaatgttg gaactcatcg
     2161 ataataatca gcttcacgcc gaccgcaggg attaattcag tcagtctttt cgtcaatcta
     2221 gctaagtcag tttcataaag tgctagcggg tcacccattt caaccagcaa ttcccttgct
     2281 gcatctactg gcttcgcatt gtctggcaac tcaatatgca aaaccggtat tatatcgccc
     2341 ccggcttcgg actctcttct gttttgattt aagtatttct tgatgacggt cgttttacct
     2401 acacccgaag caccatagac catcatgcat gtaggctcag actctaggtc tgatagatct
     2461 ctacatctat ccatgtaact caagattttc ctaacgctcg gtgtggatac aaatgccctt
     2521 ttagctcttg atatacgagc ctcacgcgtt tcactcattt gacgatttct cagacataat
     2581 caatatccca atcgtcatta tctgcactaa cgatttcatc ttcatgtttt tgtatcgagg
     2641 ccgggttcgc attactgatt gacttagccc ttgcgctatt ctcttgatgc ctagcgccac
     2701 gcctccgagc tctgattttg ttagtcttaa caattgaacg atctgcgatt tcttcaattt
     2761 ttatttctgc atcaatttcc ttgtcctcat catattctgc tgaatttagt tctgcttgat
     2821 atttcatgtt atatttatgt tgccaaagtg atactctact tgcatattcg tagtcaatcg
     2881 cattaactgt aaagtactca ttcatatcct catccaacac ccaaataact gccatacact
     2941 cagggttata cttgaactga actttatggt ttcctttctt ccctctatat tcagctaaac
     3001 ggtcattgct ataactcagt tctttgtaga cagttatccc tactttagta agttgtttgt
     3061 aatcaacaat agcaaattta aagtctaatt cgtctttaga accagagaac tcctctggtt
     3121 cccattcttg acaacccttt ttccacgcaa cattaggaca attagtgcct ctctgattgg
     3181 gttttttatg ataaatatcc accaaccaaa ttaagtaaat ttctctaatc tcattgagtg
     3241 taagggtagc ttctcccaca gagtcgtacc cttctctttg aaggtactgg ctgaaggatt
     3301 tcccaggtaa atcgtctaac agagaagtat taatagtccc gtagttacgt tcaacatgag
     3361 gtttgttgtc gggcgtctca actttatttt gatgcacatt gatcaatagt gattcacatg
     3421 cttgatcaaa tgctttcgac aaaaactctt taccattatc agttacgagt aggtctggga
     3481 tgccataaca tagccattca ttctcgatcg attcatacga ggagattaag tcatctttgc
     3541 gttgtattgc attcttaagt gcaagggaaa ccgacacata gctaggaggc tcgaaaccta
     3601 aataaaaacc gattacagct ttactgtaac aatcaaccaa ttgagtaagc caaggtcggc
     3661 ccaatgggat tcgatactct tcgtgtactg caaaaaggtc aacgacagtg tgatctattt
     3721 caaccctctc tagcacgcta gacgtgagga tcttttttcc cattcggcga aactctctct
     3781 tagctactct ctcccctttc cctgcggcta ataactcaaa tggggttttc ttttttactc
     3841 gctttcttac agattcgtac ttaggatacg tgtatttcgt tccatgagtt agattgtatt
     3901 gacgaacttt tcgtcgaaca cgtttatatg cagagctaac attgactttt cgtccagata
     3961 taactctttc aactgcctgt tccataatag aatcaacaac tgttgacact tttgtctctc
     4021 tattacctct atccttgata ttaggtgcta agctaatggg gttgtagtct gattttcgaa
     4081 aagcaagcca ccatcgatat atcgttattg cactgggtat ttttctacta tagttttgct
     4141 gtagctctac acgatgtggt tcaattgatt tggccacaat ggtttcgcac tttcgcgcta
     4201 aaaagcgaag aatgcttaat cggaaattaa tctctaccgc aattgtttcg ggaaatgctg
     4261 atatatcttg atacgtaaca ctttctagac aattagcgct agagacaagc tctatcgatt
     4321 caagcgtatc ttgggaagag actgcttttc tatggaaact tgagaaccct ttcttagcca
     4381 taactggcca actcaaatcg agattcaaga gatagaggct tatccaaacg tgtatcgagt
     4441 aaacaccttg acagcaaatc gcacaaaatt ggtaaaacag ttcggccttt taaacccaac
     4501 tgttctccca agcatcttgc agattgggcg ccattgtact ttattatttt taagagagag
     4561 ttatgtacgt tattcagctc atctttcgaa gcataacgat gcatgcgctt aagattattt
     4621 aatacaaccg tatccctaat atcactctct tcaacaagct ccaattctag ccctagttca
     4681 gttgctgctt ttactttcgc ctcccattca acatcaaaat caggtttgtt cttagcataa
     4741 gctgacttta cttcatatag aacaaactcg ttggtatcaa attgaactag aaagtcagga
     4801 acataggagt gagactgacc attgagataa tataaaaatc gaatcggttg agcgcaaaag
     4861 cgaacaatac ttggctcaaa atcaaagtga tagcaggcat cacactctag agtagattct
     4921 accgtaatcc tcttactcat ctttacactg acataacgat gaatgttttt tccgcgagat
     4981 ttcgttagat tgcgagcggg agtatggaat gcatactcta acgccgaagt cgtaattgct
     5041 gagggcgtag gtaaacttgt cgccatggta tatctcctta ttaaaggtca gattaagggt
     5101 acaggctgaa acgagttttc tgaatttgat aagccatagc tactaaaaat cgagttcccc
     5161 tcacggggtt gctccgagag catcctgata ttgagtcgaa agttgcggtt cgtctgtttg
     5221 ctggactccc caagtgagtg gtagtgcgct attgcagtat gctctttttg agcaaaagat
     5281 gatgggtcaa attgctcgcc tctagataga gcacgtttct ccaggcgtct gattttcctt
     5341 gccagccctg ctgctgtgag cttatctata gattgacagc gtctaaagga aacgtatgta
     5401 caatcttctg ggacgagaac agtgttggat atatgaaagt attgaagttg ttccatttgg
     5461 acgaaatagt gttgtttaag tagtaagtcg agttctatct tgttcttgct gacaaatgaa
     5521 atcttttttc caaccgttgc atcacaccaa agcggaaatg aaacgccgat atttcgtgtc
     5581 tcatactgat agttaaatcc atgcagaacg cggagacact ttgcagccag tgactcgttg
     5641 ttgcacaact ctggcagaaa ggtgattgtc ttataatacc atttcacagt cagtctcctt
     5701 tatgctggaa catcccaccc ttaatcaggt tagccattaa aaagtgcatg tcgttgatag
     5761 tttcttgagc gggagttttg ttggcgctca acacttcaat atagtgctct gcttgttgta
     5821 atatcgagaa aaaatctttt ccggtagacg gatgacggta gcaagtgaca tcttcgcgat
     5881 gaaccccaaa ccgtccgacc cttagtggct cagtggcttc aggataccag tcgtcaatgg
     5941 ttgcaatagc tgctcccgtt ttaaaggccc ctagtattgg cgatcgttca ccatcaatag
     6001 ttgtactctg aaaaactcga ctgttttgag ttttagattt acttttactg ccactttctt
     6061 tttctgtgaa aacttggctt ggccgcacca tagcattcgt tggcaagtgc aaggtggccc
     6121 tgacttcaaa tatcgccagc ccatcagtac tagaaaatgc ggttttgatc atttcaacta
     6181 tagcagacca atttttatta tttttaaagt cttgccgtga ggtgtagtta gtacgaatat
     6241 cttcaaatcc aaccttttcc ccgttccaag gccaaggtgt aagtacaatg ttccagcaat
     6301 aggctttacg ggtatttttc cacagccatt taccgttgca aatgttcatc aaatatcggg
     6361 ttgctagctc agtccagccg attttcgttt cgtagagctc gactaattgc actagcgttt
     6421 gtttaacttt gcttgagtta cacttatatg gttgacgtag ttcagaagaa aacgagacgg
     6481 agaaactgca ttcaatatga ctcgcaccgt atggaacgtg gcagaaatca acttgatgag
     6541 ggttcccttg agctaacgcc tcagcggtgg cactttttat tggttgacct gagacatcat
     6601 aggctaatga tgccgcttcc atttgcccga gcagagtacg agaattgtag gttaaaggtg
     6661 tttttctatc atcgggccag acgacaaaaa aacagacatc tgatgggtcg atagagcgct
     6721 cataggctag atttgtcggt agtttcatcg ttgatacttc ttggctgtac cactaggtag
     6781 ttttgatagt tctttagcga ccaaaagatt gtgttgggat cagtctctga gctaaaggtg
     6841 attgagttaa tgagtccaat gatgcactcg gcgatagcgt gtttgtaacc tctgagggag
     6901 tttggtttat ctttgggctc ttctaacaag tggtaaccga cgcagcttgc catgagctga
     6961 tggtcctctg tcatggctgc taatagatca cttagattat tgtttgactg tgcgtaaagt
     7021 gataaccacc taccagcttc tattggcaga gattcgatcg ctgtttctaa tgtcgagaat
     7081 gagttgatat gtttaaagtc atcaatcgca atttttgctg aaccccgagc caatcgcttt
     7141 ggtagtgatg taactaacgt atcttggtca atatgttgag caaagttggt gttcaaaata
     7201 aggctaaata cgacatcaca ctgccagtca tcccgggtcg cgggagcgga tatagtcccg
     7261 tcgccttttt ccacaaactc tgctgtcaaa cctcgcttct cgacatgtag ttgatggacg
     7321 catatcgcaa aggattcaac acgaaacgat gaggtggtgc gatttatatt ccgttcaaat
     7381 gcgtgaacga agccaaagaa agctgtaagt gatggaatcc ccaccgttac cggggagctt
     7441 aaagctgttg cgccacaaac cctgatattc ggtaggacta aaaacgatcc attagtgtgt
     7501 tgctcaccat tattgattga atttgataac tctcgtttca aaagctcagt aaagagcttg
     7561 gtcatcgctg gatcgtatgc aaaacgtttt gtggctccta agtaagatag gtcatgattc
     7621 aaatgttgga taagtatgtt cgaatcaagg gtatggtctt gcattgcaaa ccaagatctg
     7681 accatatttt gaagctcgat tttgtatttt ctacggagtg caatccgaga gttttcaggt
     7741 atgaggcttt tgttgaggcg ctggtactgt tttaatgact gaacatgctc tgacgtcaac
     7801 caacttcgtt tactgtttaa tcggtgatga ggggggctgg aaaacttagc gccagacttt
     7861 aacattctaa atgcgccgcc acatgtcatc gcagtgaccc ccatgttggt agttcggcta
     7921 aaccgcgtta tcgcgctatg ccgattttca tcttggagcc tctgatgaaa gtggctttgc
     7981 atcgatagcg atgcaacagg agagagtgaa atgtaggatg tatcactatc ggggagagat
     8041 atttgagtca ggtaatttgg tgcgagtgtg acattgatag tcgtgagagt aatatcagct
     8101 agttgttttg ccattgcttt gcaagttttt tgagaacaac ctaacttttt caaagtattc
     8161 caaagtgggt gatctgcatc tttaagaagc tcacccaaac atgagatctc accatcccaa
     8221 caaaactcat tggtgaggaa tgcgcttttg ttgacgtatt ttgaatcatg gctatatgac
     8281 caatggtatg gtgggatttt tgaggacgag agtaggaaac tcggtaattc acccagggct
     8341 tgggttcgaa tcacgccttc agaacgtata tccgggaatt ttgggttatg gctttgtctg
     8401 taattaacgc aatttatgca gctagcccac catttttcat ctcgaagttt ttgcttgcat
     8461 ttggctcgat cgagcaggtc tttctgatca tcagtcttat cggttaaatt gactaatatc
     8521 gtcagtgcgt caagttcatt accatcaatt gcaatatgcg gtgtgagtgg acgaaatgct
     8581 ctcttaagtt cagttgttaa gtcgtcagga ttggatgcga ttagttcttt cagagtttgc
     8641 atttaccacc tcgacacata aaacgaacgg ttaaactcat cgctttggaa ctcagctaac
     8701 caaagacaga aaatatcgcc gaaatgaaat agataatcgg taacatcaag agggctgttg
     8761 ggcttgcttt ttcgatttgg cttcaagatg cgttgttcaa ccattgatgc aatctgatcg
     8821 aggctacaat ttaacattac agtcgcctct aacgcgttca ttttgaggtt ggcgattaat
     8881 cccttgtctt gccataaacg attttctaaa tagcagagaa gctcaccaag gatgatattg
     8941 tgttgaagat tccgctcagg taaccgaatt gaaccgaaaa acaatcgacc aagaagatct
     9001 tttagtcgta attcagacgt gctgacaaca gcatgctcaa ggttgaactc tacttcatct
     9061 tcgattatcg agtggaatga ccttggccag tttgagaaaa attgaacgaa cgaaaagtga
     9121 tcgaactcgc tatctttaat acccatccac caatgaacta aaccccatcg gtagctttta
     9181 ggaagatttg gcagaggttt agattcatga ccagcaagcc agtttgaaac agtacacgct
     9241 gcctcatgac cgttctccct gctggttaag gtgataggct ccttgcattt gcagcaaatg
     9301 ccctttaacc cagatactcg gtagtcaaac tccttaccac agctacaagt ggtaattaaa
     9361 ggtacgttat ggctgtggca gtattcgtac ccctgaaagt gccaaaggta ggaggcgtag
     9421 ccattttctc gcagacacaa aggacagcag gggatggagt gcgtccgtag taaactgcga
     9481 ggaaagactt ctgcacctct aacaaccgcg ctagttgacg gcgagtattt catgtttgtt
     9541 ctgttaattg ccaacccaag tagctctggc ggttcattaa atgtcaattg tgcaagtttc
     9601 aggaatgacg cagttcgtgc gctggaactg tttttagctg agtatgggtt tatccgagtt
     9661 atatcagtcg gaaaggtttg atagccatta tggtcaatgt cttgaaggaa tcgtttagtg
     9721 gcttctagga agcgatggac atcaccgtag ccatttttgt tagccactcg gataaagaaa
     9781 ctttctaaac tttcatcgct gtaaggttta ggtctttgca aaaacatatg tatatctcct
     9841 tcgctcgaat tcggatccag ggatgtatat ctccttcgct cgaattcgga tccagggatg
     9901 tatatctcct tcgctcgaat tcggatccat aggagaccat gcctggtcat ttgtacagtt
     9961 catccatacc atgcgtgatg cccgctgcgg ttacgaactc cagcagaacc atatgatcgc
    10021 gtttctcgtt cggatcttta gacagaacgc tttgcgtgct cagatagtga ttgtctggca
    10081 gcagaacagg accatcaccg attggagtgt tttgctggta gtgatcagcc agctgcacgc
    10141 tgccatcctc cacgttgtgg cgaattttaa aattcgcttt aatgccattt ttttgtttat
    10201 cggcggtgat gtaaacattg tggctgttaa aattgtattc cagcttatgg cccaggatat
    10261 tgccgtcctc tttaaagtca atgcctttca gctcaatgcg gtttaccagg gtatcgcctt
    10321 caaatttcac ttccgcacgc gttttgtacg tgccgtcatc cttaaaggaa atcgtgcgtt
    10381 cctgcacata gccttccggc atggcggact tgaagaagtc atgctgcttc atatggtccg
    10441 gataacgagc aaagcactga acaccataag tcagcgtcgt taccagagtc ggccaaggaa
    10501 ccggcagttt accagtagta cagatgaact tcagcgtcag tttaccatta gttgcgtcac
    10561 cttcaccctc gccacgcacg gaaaacttat gaccgttgac atcaccatcc agttccacca
    10621 gaatagggac gacaccagtg aacagctctt cgcctttacg catctagtat ttctcctctt
    10681 tctctagtag ctaagatccg aacctgccgt ttcttgagtt gccgcgatgg gtctctcgtt
    10741 tggtatatct ccacgtcgac gctagtatta tacctaggac tgagctagct gtcaacctcg
    10801 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    10861 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat ttaaggtata
    10921 ctttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
    10981 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
    11041 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
    11101 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
    11161 ccgataagct ctgataacgc aggaaagaac atgggcagtt caacctgttg atagtacgta
    11221 ctaagctctc atgtttcacg tactaagctc tcaagtttaa cgtactaagc tctcatgttt
    11281 aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc
    11341 atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta
    11401 aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc
    11461 ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta
    11521 gagcctctca aagcaatttt gagtgacaca ggaacactta acggctgaca accggctggt
    11581 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    11641 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    11701 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    11761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    11821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    11881 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    11941 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    12001 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    12061 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    12121 gagccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    12181 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    12241 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    12301 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12361 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12421 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    12481 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    12541 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    12601 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    12661 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    12721 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    12781 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    12841 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    12901 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    12961 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    13021 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    13081 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    13141 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    13201 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    13261 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    13321 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    13381 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgatcat
    13441 acacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    13501 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    13561 aaaacctctg acacatgcag ctcccggtcc cagtcacgac gttgtaaaac gacggccagt
    13621 gaattcgagc tcggtacctg ttgatgcaac cataaagtga tatttaataa ttatttataa
    13681 tcagcaactt aaccacaaaa caaccatata ttgatatctc acaaaaca
//
