LOCUS       sequence-412254-       12412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibly expresses human Caspase 8 fused to light-activatable Cry2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12412)
  AUTHORS   Shkarina K, Hasel de Carvalho E, Santos JC, Ramos S, Leptin M, Broz 
            P
  TITLE     Optogenetic activators of apoptosis, necroptosis, and pyroptosis.
  JOURNAL   J Cell Biol. 2022 Jun 6;221(6). pii: 213145. doi: 
            10.1083/jcb.202109038. Epub 2022 Apr 14.
  PUBMED    35420640
REFERENCE   2  (bases 1 to 12412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-06-6"; volume: "221(6). pii: 213145. doi"; 
            pages: " 10.1083/jcb.202109038. Epub 2022 Apr 14"
COMMENT     Sequence Label: pLVX-opto-hCaspase-9
FEATURES             Location/Qualifiers
     source          1..12412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1773..1907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             2075..2708
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2755..2880
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3377..3610
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3795..3839
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4102..4217
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     polyA_signal    4261..4395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             5111..5122
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(5612..7105)
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     CDS             complement(7121..7828)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLRDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7396..7415)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7665..7683
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(7834..8201)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    7959..7977
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7995..8013
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8031..8049
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8067..8085
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8103..8121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8139..8157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8175..8193
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        8218..8728
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8647..8665
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8747..9493
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        9504..9833
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9684..9819
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9842..10441
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9842..9861)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10338..10358
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10455..11043
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10508..10528)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10926..10937)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11250..11883
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12011..12027)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12011..12027)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12024..12046)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12035..12051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12059..12065,12066..12083,12084..12089))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12104..12125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12242..12259)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt
     1801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     1861 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     1921 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     1981 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     2041 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
     2101 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     2161 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     2221 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     2281 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     2341 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     2401 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     2461 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     2521 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2581 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2641 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2701 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     2761 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     2821 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     2881 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     2941 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3001 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3061 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3121 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3181 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     3241 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     3301 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     3361 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     3421 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     3481 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     3541 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     3601 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     3661 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     3721 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     3781 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     3841 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     3901 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     3961 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     4021 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     4081 acggatctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta cagtgcaggg
     4141 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     4201 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttatcgactt
     4261 aacttgttta ttgcagctta taatggttac aaataaggca atagcatcac aaatttcaca
     4321 aataaggcat ttttttcact gcattctagt tttggtttgt ccaaactcat caatgtatct
     4381 tatcatgtct ggatctcaaa tccctcggaa gctgcgcctg tcttaggttg gagtgataca
     4441 tttttatcac ttttacccgt ctttggatta ggcagtagct ctgacggccc tcctgtctta
     4501 ggttagtgaa aaatgtcact ctcttacccg tcattggctg tccagcttag ctcgcagggg
     4561 aggtggtctg gatccgccgg caccggtgta tacgggaatt ttatgatgtt ttaaagaaaa
     4621 gttttttccg gaggaaatta aagcaaccag gcatctgttt ataaatccct ttcaccgaaa
     4681 cagcattagc gaccctaagc aggagggact gcaggtcttc agagtgagcc cactgctcaa
     4741 agatgtcgtc cagggtctca acgtaccagg agccactctt ggggtccctc caggaaacaa
     4801 aacctgggaa agtagagtag gacacaaaga tgtcactggg tgtgggcaaa ctagatatgg
     4861 cgtccagctg gtcgaaggtc ctcaaacctt cctggaacgg ggtggcatct ggctcggggt
     4921 tactgccagg ggactcgtct tcaggggaag tggaggccac ctcaaaccca tggtctttct
     4981 gctccccacc acaggcctgg atgaaaaaga gcttgggctt ccctcccagg ctggggcagc
     5041 tggtcccatt gaagatgttc acaatcttct cgaccgacac agggcatcca tctgtgccgt
     5101 agacagcccc tgggaactgc aggtggctgg cctgacagcc gtgagagaga atgaccacca
     5161 cgcagcagtc cagagcaccg tggtcctgcc gcgccagctc cagcaaagcc agcaccattt
     5221 tcttggcagt caggtcgccc ttcacctcca ccatgaaatg cagcgaggag aagcgacgcc
     5281 gcaacttctc acagtcgatg ttggagccag tgcgggtgcg gagcccggac tcacggcaga
     5341 agttcacatt gttgataatg aggcagtggc cacagggctc catgctcagg atgtaagcca
     5401 aatctgcatt tcccctcaaa ctctcaagag caccgacatc accgaatcct ccagaaccaa
     5461 tgtccactgg tctgggtgtt tccggtctga gaacctctgg tttgcgaatc tctggtctga
     5521 gcaccactgg ggtaaggttt tctagggttg gcttcgacaa ctttgctgct tgcctgtttc
     5581 ctcctccggt ggcgaccggt ggatcccggg ctgctgctcc gatcatgatc tgtgctccac
     5641 gggttcttga aatagcttta gctagtagct cacgagctgt gtcgatgtct acaatgggtt
     5701 tcgcatagtt tgttccgagt tccacaccag aagctttgag tacggttaaa ggagcgtccc
     5761 atggatgatg gatccattca gttggcaatc tcgcaagctc gggaagccat tgccttatgt
     5821 actcaccttc tgggtcatat ttggcgcctt gtaacgcggg attgtccaag cgatcaagct
     5881 cgtggccatc ggggatactc ccagagatat actgccagcc aaggatgtca cattccaaat
     5941 cagcatccaa aagtgtatcc cagaaatact tcattcccca tttccatgga aggagaagaa
     6001 acttcacagc aaagcttgaa acaatcactc ttattctgtt atgcatccat ccggtagccc
     6061 aaagctctct cattccggca tccaccaacg gataaccggt cctgccttgt ctccaggcct
     6121 tgaacttatc aacatcagca tcccaaggga aaaaccgaag atgactcaac aacgattgct
     6181 cgtgagtaaa cgggaagttg aaacatatat accgagaata ctctcttaaa ccgattcccc
     6241 taagaaaaag atctgcactt tcttctcctt cactgttctt atctcttgcc catataattt
     6301 gtttcatccg ggcacactgg aaaacgtgtc tgacgcttat ttccccgaaa tggagatacg
     6361 gagaaagtag tgaagtagaa ttcccaacaa ctttcttgct gttctttgca taatctatca
     6421 actgcttctc gatgaactca tttagtaact tatcagcatt gctccatcct ggagaccaag
     6481 ctctagttaa caacgcattg ctcggtttct cggcctcatt ctccagccct agttcttcaa
     6541 tcgaacacgc ccaaatcgct tcagccgctg cagttattgg catcaaccgc caaggaggag
     6601 gaagcataac ggattcaatc gacatatcta agcatttctt ccagtaagaa ttgaaactcg
     6661 taaaaggttt gcccttttcg cagtatatct cccacggttc atacaataga tctccattgt
     6721 agctttgcac agagatccca cgttccacca gcttctcctt tacggtatgg tcccgaacta
     6781 acgaaacagg atcatagagg tggttaaaga cgacttttgt agcaccggta acgcggatac
     6841 aatccaagat cgctgaaatc gtgttgtggg ttttgattaa agtgaggtca gatccaagag
     6901 ccttcaagga ttgagataag tgagcaagtg attgtttcat ccaccatctt gaagctcttc
     6961 caggataaaa ctgtccttct tcttcaggac accaaatgaa gacaggaaaa acagatcctt
     7021 cgtgagcagc tgctgctaat gcaggattat cctcaatcct taggtctctt ctaaaccaaa
     7081 ctatagtctt tttgtccatc ttcatcattc ctcctcctcc cttgtacagc tcgtccatgc
     7141 cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt
     7201 tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct
     7261 tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ctcagcttca
     7321 gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg
     7381 cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc
     7441 gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca
     7501 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
     7561 gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga
     7621 actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct
     7681 tggcggtctg ggtgccctcg tatgggcggc cctcgccctc gccctcgatc tcgaactcgt
     7741 ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca
     7801 tgttatcctc ctcgcccttg ctcaccataa ttctttacga gggtaggaag tggtacggaa
     7861 agttggtata agacaaaagt gttgtggaat tgaagtttac tcaaaaaatc agcactcttt
     7921 tataggcgcc ctggtttaca taagcaaagc ttatacgttc tctatcactg atagggagta
     7981 aactggatat acgttctcta tcactgatag ggagtaaact gtagatacgt tctctatcac
     8041 tgatagggag taaactggtc atacgttctc tatcactgat agggagtaaa ctccttatac
     8101 gttctctatc actgataggg agtaaagtct gcatacgttc tctatcactg atagggagta
     8161 aactcttcat acgttctcta tcactgatag ggagtaaact cgaggtgata attccacggg
     8221 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     8281 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     8341 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     8401 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     8461 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     8521 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     8581 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     8641 cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt
     8701 tgaccgaatc accgacctct ctccccaggg ggatcatcga attaccatgt ctagactgga
     8761 caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta tcgaaggcct
     8821 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
     8881 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
     8941 ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
     9001 ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
     9061 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
     9121 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
     9181 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
     9241 tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag ccgaacctgc
     9301 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
     9361 cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
     9421 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
     9481 gctccccggg taaacgcgcg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     9541 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     9601 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     9661 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     9721 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     9781 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaacgcgac
     9841 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     9901 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     9961 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
    10021 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
    10081 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
    10141 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
    10201 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
    10261 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
    10321 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
    10381 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
    10441 aacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
    10501 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
    10561 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
    10621 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
    10681 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
    10741 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
    10801 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
    10861 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
    10921 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
    10981 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
    11041 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
    11101 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
    11161 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
    11221 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
    11281 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
    11341 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
    11401 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
    11461 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
    11521 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
    11581 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
    11641 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
    11701 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    11761 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    11821 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    11881 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
    11941 cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg
    12001 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    12061 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    12121 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    12181 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    12241 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    12301 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    12361 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tt
//
