LOCUS       sequence-412255-       12586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  R6K plasmid with a I-F CAST targeting lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12586)
  TITLE     Distinct horizontal transfer mechanisms for type I and type V 
            CRISPR-associated transposons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pR6K-crRNA-CASTIF
FEATURES             Location/Qualifiers
     source          1..12586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(147..938,939..1007))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     770..789
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1008..1112)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1180..1198
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1236..1258)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1260..1282
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1275..1291
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1511..1597
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1689..1716
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    complement(1794..1841)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1858..2517)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2432..2451
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2518..2620)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(2888..2935)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     2931..2949
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(4384..4395)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(11506..11540)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     complement(11551..11568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     oriT            11809..11917
                     /note="incP origin of transfer"
     rep_origin      11968..12324
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
       61 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
      181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      241 atacgagagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
      421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
      481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
      541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
      601 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
      661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
      721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
      781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
      841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
      901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
      961 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1141 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1201 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggtc
     1261 ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtacct gttgatgcaa
     1321 ccataaagtg atatttaata attatttata atcagcaact taaccacaaa acaaccatat
     1381 attgatatct cacaaaacaa ccataagttg atatttttgt gaatcgagta tttcagcaaa
     1441 actactgcag taaggactct agaagggcac caataactgc cttaaaaaaa gcagctatgc
     1501 cggtctctcc caaataaaat gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1561 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     1621 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
     1681 aattaagcag aaggccatcc tgacggatgg cctttttgcg tggccagtgc caagcttgca
     1741 tgcagattgc agcattacac gtcttgagcg attgtgtagg ctggagctgc ttcgaagttc
     1801 ctatactttc tagagaatag gaacttcgga ataggaactt catttaaatg gcgcgcctta
     1861 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     1921 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     1981 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     2041 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     2101 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     2161 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     2221 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     2281 gtctttcatt gccatacgta attccggatg agcattcatc aggcgggcaa gaatgtgaat
     2341 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     2401 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     2461 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     2521 agcttcctta gctcctgaaa atctcgacaa ctcaaaaaat acgcccggta gtgatcttat
     2581 ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa
     2641 gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg
     2701 atcttccgtc acagagacca ctggccgtcg acaggatccc cggaaatgac ctcgaggaaa
     2761 atgtcgtaaa cttactgaag tttagaccat gaagaggcaa tatcaattta tgggtgtgat
     2821 aattatcaat ttatgggtgt aattatcatt ttatggttgt atcaacaaca aatgcctgag
     2881 gtttcagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttatt
     2941 gctcagcggt ggcagcagcg cagcagccta ggttattcga aaaagttttt atttctacct
     3001 ggtttactca ctctcggggt tgaatgtctc aaatagtcct cccaaccaat atcttttggt
     3061 ggcggtcgta acatatcttc tgttacctca acaccctcct caaaaggatt tttccatgag
     3121 ttgggtttat cgcccgatgt caacttcgat gcaaaaacga aatcttcttc ggttatcgtc
     3181 tcatgctgat tatcaattgc ttcgatagat gcttgataaa taaggtttct caacgaacgc
     3241 atgtttccct gagagaatgc atacaatttt ttctgcaaac tttcgttggc taagccagcc
     3301 tgtttttcaa aaggtagggc tttatcaagg tattccaaaa aagttttaaa tacacctcta
     3361 cctccctggt agctaaaggg gcgcagttca acctgaatgg aaaatcgccc gtgaagttgc
     3421 gagtttgctt gcagtacaac ttttgagtat ggcataccaa atataacaat tggacatttc
     3481 gttttgttaa gtatcatttt tagccaatta cctacttggg taagaacccg atttgacctt
     3541 tcttccacca aatgttggaa ctcatcgata ataatcagct tcacgccgac cgcagggatt
     3601 aattcagtca gtcttttcgt caatctagct aagtcagttt cataaagtgc tagcgggtca
     3661 cccatttcaa ccagcaattc ccttgctgca tctactggct tcgcattgtc tggcaactca
     3721 atatgcaaaa ccggtattat atcgcccccg gcttcggact ctcttctgtt ttgatttaag
     3781 tatttcttga tgacggtcgt tttacctaca cccgaagcac catagaccat catgcatgta
     3841 ggctcagact ctaggtctga tagatctcta catctatcca tgtaactcaa gattttccta
     3901 acgctcggtg tggatacaaa tgccctttta gctcttgata tacgagcctc acgcgtttca
     3961 ctcatttgac gatttctcag acataatcaa tatcccaatc gtcattatct gcactaacga
     4021 tttcatcttc atgtttttgt atcgaggccg ggttcgcatt actgattgac ttagcccttg
     4081 cgctattctc ttgatgccta gcgccacgcc tccgagctct gattttgtta gtcttaacaa
     4141 ttgaacgatc tgcgatttct tcaattttta tttctgcatc aatttccttg tcctcatcat
     4201 attctgctga atttagttct gcttgatatt tcatgttata tttatgttgc caaagtgata
     4261 ctctacttgc atattcgtag tcaatcgcat taactgtaaa gtactcattc atatcctcat
     4321 ccaacaccca aataactgcc atacactcag ggttatactt gaactgaact ttatggtttc
     4381 ctttcttccc tctatattca gctaaacggt cattgctata actcagttct ttgtagacag
     4441 ttatccctac tttagtaagt tgtttgtaat caacaatagc aaatttaaag tctaattcgt
     4501 ctttagaacc agagaactcc tctggttccc attcttgaca accctttttc cacgcaacat
     4561 taggacaatt agtgcctctc tgattgggtt ttttatgata aatatccacc aaccaaatta
     4621 agtaaatttc tctaatctca ttgagtgtaa gggtagcttc tcccacagag tcgtaccctt
     4681 ctctttgaag gtactggctg aaggatttcc caggtaaatc gtctaacaga gaagtattaa
     4741 tagtcccgta gttacgttca acatgaggtt tgttgtcggg cgtctcaact ttattttgat
     4801 gcacattgat caatagtgat tcacatgctt gatcaaatgc tttcgacaaa aactctttac
     4861 cattatcagt tacgagtagg tctgggatgc cataacatag ccattcattc tcgatcgatt
     4921 catacgagga gattaagtca tctttgcgtt gtattgcatt cttaagtgca agggaaaccg
     4981 acacatagct aggaggctcg aaacctaaat aaaaaccgat tacagcttta ctgtaacaat
     5041 caaccaattg agtaagccaa ggtcggccca atgggattcg atactcttcg tgtactgcaa
     5101 aaaggtcaac gacagtgtga tctatttcaa ccctctctag cacgctagac gtgaggatct
     5161 tttttcccat tcggcgaaac tctctcttag ctactctctc ccctttccct gcggctaata
     5221 actcaaatgg ggttttcttt tttactcgct ttcttacaga ttcgtactta ggatacgtgt
     5281 atttcgttcc atgagttaga ttgtattgac gaacttttcg tcgaacacgt ttatatgcag
     5341 agctaacatt gacttttcgt ccagatataa ctctttcaac tgcctgttcc ataatagaat
     5401 caacaactgt tgacactttt gtctctctat tacctctatc cttgatatta ggtgctaagc
     5461 taatggggtt gtagtctgat tttcgaaaag caagccacca tcgatatatc gttattgcac
     5521 tgggtatttt tctactatag ttttgctgta gctctacacg atgtggttca attgatttgg
     5581 ccacaatggt ttcgcacttt cgcgctaaaa agcgaagaat gcttaatcgg aaattaatct
     5641 ctaccgcaat tgtttcggga aatgctgata tatcttgata cgtaacactt tctagacaat
     5701 tagcgctaga gacaagctct atcgattcaa gcgtatcttg ggaagagact gcttttctat
     5761 ggaaacttga gaaccctttc ttagccataa ctggccaact caaatcgaga ttcaagagat
     5821 agaggcttat ccaaacgtgt atcgagtaaa caccttgaca gcaaatcgca caaaattggt
     5881 aaaacagttc ggccttttaa acccaactgt tctcccaagc atcttgcaga ttgggcgcca
     5941 ttgtacttta ttatttttaa gagagagtta tgtacgttat tcagctcatc tttcgaagca
     6001 taacgatgca tgcgcttaag attatttaat acaaccgtat ccctaatatc actctcttca
     6061 acaagctcca attctagccc tagttcagtt gctgctttta ctttcgcctc ccattcaaca
     6121 tcaaaatcag gtttgttctt agcataagct gactttactt catatagaac aaactcgttg
     6181 gtatcaaatt gaactagaaa gtcaggaaca taggagtgag actgaccatt gagataatat
     6241 aaaaatcgaa tcggttgagc gcaaaagcga acaatacttg gctcaaaatc aaagtgatag
     6301 caggcatcac actctagagt agattctacc gtaatcctct tactcatctt tacactgaca
     6361 taacgatgaa tgttttttcc gcgagatttc gttagattgc gagcgggagt atggaatgca
     6421 tactctaacg ccgaagtcgt aattgctgag ggcgtaggta aacttgtcgc catggtatat
     6481 ctccttatta aaggtcagat taagggtaca ggctgaaacg agttttctga atttgataag
     6541 ccatagctac taaaaatcga gttcccctca cggggttgct ccgagagcat cctgatattg
     6601 agtcgaaagt tgcggttcgt ctgtttgctg gactccccaa gtgagtggta gtgcgctatt
     6661 gcagtatgct ctttttgagc aaaagatgat gggtcaaatt gctcgcctct agatagagca
     6721 cgtttctcca ggcgtctgat tttccttgcc agccctgctg ctgtgagctt atctatagat
     6781 tgacagcgtc taaaggaaac gtatgtacaa tcttctggga cgagaacagt gttggatata
     6841 tgaaagtatt gaagttgttc catttggacg aaatagtgtt gtttaagtag taagtcgagt
     6901 tctatcttgt tcttgctgac aaatgaaatc ttttttccaa ccgttgcatc acaccaaagc
     6961 ggaaatgaaa cgccgatatt tcgtgtctca tactgatagt taaatccatg cagaacgcgg
     7021 agacactttg cagccagtga ctcgttgttg cacaactctg gcagaaaggt gattgtctta
     7081 taataccatt tcacagtcag tctcctttat gctggaacat cccaccctta atcaggttag
     7141 ccattaaaaa gtgcatgtcg ttgatagttt cttgagcggg agttttgttg gcgctcaaca
     7201 cttcaatata gtgctctgct tgttgtaata tcgagaaaaa atcttttccg gtagacggat
     7261 gacggtagca agtgacatct tcgcgatgaa ccccaaaccg tccgaccctt agtggctcag
     7321 tggcttcagg ataccagtcg tcaatggttg caatagctgc tcccgtttta aaggccccta
     7381 gtattggcga tcgttcacca tcaatagttg tactctgaaa aactcgactg ttttgagttt
     7441 tagatttact tttactgcca ctttcttttt ctgtgaaaac ttggcttggc cgcaccatag
     7501 cattcgttgg caagtgcaag gtggccctga cttcaaatat cgccagccca tcagtactag
     7561 aaaatgcggt tttgatcatt tcaactatag cagaccaatt tttattattt ttaaagtctt
     7621 gccgtgaggt gtagttagta cgaatatctt caaatccaac cttttccccg ttccaaggcc
     7681 aaggtgtaag tacaatgttc cagcaatagg ctttacgggt atttttccac agccatttac
     7741 cgttgcaaat gttcatcaaa tatcgggttg ctagctcagt ccagccgatt ttcgtttcgt
     7801 agagctcgac taattgcact agcgtttgtt taactttgct tgagttacac ttatatggtt
     7861 gacgtagttc agaagaaaac gagacggaga aactgcattc aatatgactc gcaccgtatg
     7921 gaacgtggca gaaatcaact tgatgagggt tcccttgagc taacgcctca gcggtggcac
     7981 tttttattgg ttgacctgag acatcatagg ctaatgatgc cgcttccatt tgcccgagca
     8041 gagtacgaga attgtaggtt aaaggtgttt ttctatcatc gggccagacg acaaaaaaac
     8101 agacatctga tgggtcgata gagcgctcat aggctagatt tgtcggtagt ttcatcgttg
     8161 atacttcttg gctgtaccac taggtagttt tgatagttct ttagcgacca aaagattgtg
     8221 ttgggatcag tctctgagct aaaggtgatt gagttaatga gtccaatgat gcactcggcg
     8281 atagcgtgtt tgtaacctct gagggagttt ggtttatctt tgggctcttc taacaagtgg
     8341 taaccgacgc agcttgccat gagctgatgg tcctctgtca tggctgctaa tagatcactt
     8401 agattattgt ttgactgtgc gtaaagtgat aaccacctac cagcttctat tggcagagat
     8461 tcgatcgctg tttctaatgt cgagaatgag ttgatatgtt taaagtcatc aatcgcaatt
     8521 tttgctgaac cccgagccaa tcgctttggt agtgatgtaa ctaacgtatc ttggtcaata
     8581 tgttgagcaa agttggtgtt caaaataagg ctaaatacga catcacactg ccagtcatcc
     8641 cgggtcgcgg gagcggatat agtcccgtcg cctttttcca caaactctgc tgtcaaacct
     8701 cgcttctcga catgtagttg atggacgcat atcgcaaagg attcaacacg aaacgatgag
     8761 gtggtgcgat ttatattccg ttcaaatgcg tgaacgaagc caaagaaagc tgtaagtgat
     8821 ggaatcccca ccgttaccgg ggagcttaaa gctgttgcgc cacaaaccct gatattcggt
     8881 aggactaaaa acgatccatt agtgtgttgc tcaccattat tgattgaatt tgataactct
     8941 cgtttcaaaa gctcagtaaa gagcttggtc atcgctggat cgtatgcaaa acgttttgtg
     9001 gctcctaagt aagataggtc atgattcaaa tgttggataa gtatgttcga atcaagggta
     9061 tggtcttgca ttgcaaacca agatctgacc atattttgaa gctcgatttt gtattttcta
     9121 cggagtgcaa tccgagagtt ttcaggtatg aggcttttgt tgaggcgctg gtactgtttt
     9181 aatgactgaa catgctctga cgtcaaccaa cttcgtttac tgtttaatcg gtgatgaggg
     9241 gggctggaaa acttagcgcc agactttaac attctaaatg cgccgccaca tgtcatcgca
     9301 gtgaccccca tgttggtagt tcggctaaac cgcgttatcg cgctatgccg attttcatct
     9361 tggagcctct gatgaaagtg gctttgcatc gatagcgatg caacaggaga gagtgaaatg
     9421 taggatgtat cactatcggg gagagatatt tgagtcaggt aatttggtgc gagtgtgaca
     9481 ttgatagtcg tgagagtaat atcagctagt tgttttgcca ttgctttgca agttttttga
     9541 gaacaaccta actttttcaa agtattccaa agtgggtgat ctgcatcttt aagaagctca
     9601 cccaaacatg agatctcacc atcccaacaa aactcattgg tgaggaatgc gcttttgttg
     9661 acgtattttg aatcatggct atatgaccaa tggtatggtg ggatttttga ggacgagagt
     9721 aggaaactcg gtaattcacc cagggcttgg gttcgaatca cgccttcaga acgtatatcc
     9781 gggaattttg ggttatggct ttgtctgtaa ttaacgcaat ttatgcagct agcccaccat
     9841 ttttcatctc gaagtttttg cttgcatttg gctcgatcga gcaggtcttt ctgatcatca
     9901 gtcttatcgg ttaaattgac taatatcgtc agtgcgtcaa gttcattacc atcaattgca
     9961 atatgcggtg tgagtggacg aaatgctctc ttaagttcag ttgttaagtc gtcaggattg
    10021 gatgcgatta gttctttcag agtttgcatt taccacctcg acacataaaa cgaacggtta
    10081 aactcatcgc tttggaactc agctaaccaa agacagaaaa tatcgccgaa atgaaataga
    10141 taatcggtaa catcaagagg gctgttgggc ttgctttttc gatttggctt caagatgcgt
    10201 tgttcaacca ttgatgcaat ctgatcgagg ctacaattta acattacagt cgcctctaac
    10261 gcgttcattt tgaggttggc gattaatccc ttgtcttgcc ataaacgatt ttctaaatag
    10321 cagagaagct caccaaggat gatattgtgt tgaagattcc gctcaggtaa ccgaattgaa
    10381 ccgaaaaaca atcgaccaag aagatctttt agtcgtaatt cagacgtgct gacaacagca
    10441 tgctcaaggt tgaactctac ttcatcttcg attatcgagt ggaatgacct tggccagttt
    10501 gagaaaaatt gaacgaacga aaagtgatcg aactcgctat ctttaatacc catccaccaa
    10561 tgaactaaac cccatcggta gcttttagga agatttggca gaggtttaga ttcatgacca
    10621 gcaagccagt ttgaaacagt acacgctgcc tcatgaccgt tctccctgct ggttaaggtg
    10681 ataggctcct tgcatttgca gcaaatgccc tttaacccag atactcggta gtcaaactcc
    10741 ttaccacagc tacaagtggt aattaaaggt acgttatggc tgtggcagta ttcgtacccc
    10801 tgaaagtgcc aaaggtagga ggcgtagcca ttttctcgca gacacaaagg acagcagggg
    10861 atggagtgcg tccgtagtaa actgcgagga aagacttctg cacctctaac aaccgcgcta
    10921 gttgacggcg agtatttcat gtttgttctg ttaattgcca acccaagtag ctctggcggt
    10981 tcattaaatg tcaattgtgc aagtttcagg aatgacgcag ttcgtgcgct ggaactgttt
    11041 ttagctgagt atgggtttat ccgagttata tcagtcggaa aggtttgata gccattatgg
    11101 tcaatgtctt gaaggaatcg tttagtggct tctaggaagc gatggacatc accgtagcca
    11161 tttttgttag ccactcggat aaagaaactt tctaaacttt catcgctgta aggtttaggt
    11221 ctttgcaaaa acatatgtat atctccttcg ctcgaattcg gatcccatag gagactaagc
    11281 attatgcggc cgcaagcttg tcgacctgca ggcgcgccga gctcgaattc ggatccgtta
    11341 tcagctacct actcggcagt tcaccgcttt aatgatgatt tcagccgcgc tgtactgtta
    11401 tcagctacct actcggcagt tcacccatgg tatatctcct tattaaagtt aaacaaaatt
    11461 atttctacag gggtctctcg ttccatggta tatctccacg tcgacgctag tattatacct
    11521 aggactgagc tagctgtcaa agcttgtgtc ctcgctcact gactcgctgc gctcggtcgt
    11581 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    11641 aggggataac gcaggaaaga acatttaagg tatactttcc gctgcataac cctgcttcgg
    11701 ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg
    11761 ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg
    11821 tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc
    11881 ggtgctcaac gggaatcctg ctctgcgagg ctggccgata agctctgata acgcaggaaa
    11941 gaacatgggc agttcaacct gttgatagta cgtactaagc tctcatgttt cacgtactaa
    12001 gctctcaagt ttaacgtact aagctctcat gtttaacgaa ctaaaccctc atggctaacg
    12061 tactaagctc tcatggctaa cgtactaagc tctcatgttt cacgtactaa gctctcatgt
    12121 ttgaacaata aaattaatat aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta
    12181 taagaaaaaa aagaatatat aaggctttta aagcttttaa ggtttaacgg ttgtggacaa
    12241 caagccaggg atgtaacgca ctgagaagcc cttagagcct ctcaaagcaa ttttgagtga
    12301 cacaggaaca cttaacggct gacaaccggt tggtaacagg attagcagag cgaggtatgt
    12361 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    12421 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    12481 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    12541 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacg
//
