LOCUS       sequence-412257-       12988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducibly expresses human RIP3, lacking RHIM domain, fused to 
            light-activatable Cry2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12988)
  AUTHORS   Shkarina K, Hasel de Carvalho E, Santos JC, Ramos S, Leptin M, Broz 
            P
  TITLE     Optogenetic activators of apoptosis, necroptosis, and pyroptosis.
  JOURNAL   J Cell Biol. 2022 Jun 6;221(6). pii: 213145. doi: 
            10.1083/jcb.202109038. Epub 2022 Apr 14.
  PUBMED    35420640
REFERENCE   2  (bases 1 to 12988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-06-6"; volume: "221(6). pii: 213145. doi"; 
            pages: " 10.1083/jcb.202109038. Epub 2022 Apr 14"
COMMENT     Sequence Label: pLVX-opto-hRIP3 dRHIM
FEATURES             Location/Qualifiers
     source          1..12988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             1119..1752
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1959..2547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2065..2076
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2474..2494
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2561..3160)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2644..2664)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3141..3160
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3169..3498)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3183..3318
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(3509..4255)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(4274..4784)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4337..4355)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4801..5168
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    4809..4827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4845..4863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4881..4899
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4917..4935
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4953..4971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4989..5007
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5025..5043
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6752..8242
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="KMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQF
                     YPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLYD
                     PVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIE
                     SVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLNE
                     FIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEE
                     SADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLV
                     DAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQYI
                     SGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKAS
                     GVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     regulatory      8261..8270
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8267..8977
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8412..8430)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8680..8699
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    9183..9317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    9361..9476
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(9739..9783)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9968..10201
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10698..10823
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10870..11503
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    11671..11805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        11853..11957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11958..12026,12027..12818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12176..12195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtaaa cgctagcaat gtcaaggcct ctcactctct gatattcatt tctttgcaag
     1081 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     1141 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1201 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1261 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     1321 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     1381 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     1441 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     1501 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     1561 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     1621 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     1681 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     1741 ttagcccttc cagtcccctc ttttctttta aaaagtggct aagatctaca gctgccttgt
     1801 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     1861 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     1921 catgtttttc taggtctcga ctgcagaatt aattccaggc ggggaggcgg cccaaaggga
     1981 gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag
     2041 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
     2101 gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca gcttccccga
     2161 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
     2221 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
     2281 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
     2341 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
     2401 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
     2461 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
     2521 acaaattttg taatccagag gttgattgtt ccagacgcgt tcaggcaccg ggcttgcggg
     2581 tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga
     2641 gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg
     2701 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
     2761 ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
     2821 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
     2881 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
     2941 ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
     3001 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccgggtcg acggtgtggc
     3061 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggcccgg gggacgtcgt
     3121 cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggtcgcgttt tgcaaaagcc
     3181 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     3241 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     3301 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     3361 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     3421 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     3481 acaccctaac tgacacacat tcgcgcgttt acccggggag catgtcaagg tcaaaatcgt
     3541 caagagcgtc agcaggcagc atatcaaggt caaagtcgtc aagggcatcg gctgggagca
     3601 tgtctaagtc aaaatcgtca agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt
     3661 tctccaggcc acatatgatt agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc
     3721 ggtcgaacag ctcaattgct tgtttcagaa gtgggggcat agaatcggtg gtaggtgtct
     3781 ctctttcctc ttttgctact tgatgctcct gttcctccaa tacgcagccc agtgtaaagt
     3841 ggcccacggc ggacagagcg tacagtgcgt tctccaggga gaagccttgc tgacacagga
     3901 acgcgagctg attttccagg gtttcgtact gtttctctgt tgggcgggtg ccgagatgca
     3961 ctttagcccc gtcgcgatgt gagaggagag cacagcggta tgacttggcg ttgttccgca
     4021 gaaagtcttg ccatgactcg ccttccaggg ggcaggagtg ggtatgatgc ctgtccagca
     4081 tctcgattgg cagggcatcg agcagggccc gcttgttctt cacgtgccag tacagggtag
     4141 gctgctcaac tcccagcttt tgagcgagtt tccttgtcgt caggccttcg ataccgactc
     4201 cattgagtaa ttccagagca gagtttatga ctttgctctt gtccagtcta gacatggtaa
     4261 ttcgatgatc cccctgggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg
     4321 acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac agggcccaca
     4381 ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc
     4441 ctgctgagca gccgctattg gccacagccc atcgcggtcg gcgcgctgcc attgctccct
     4501 ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt cacgtccggc
     4561 acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg
     4621 ggcccacaag ggtagcggcg aagatccggg tgacgctgcg aacggacgtg aagaatgtgc
     4681 gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct
     4741 gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa ccccgtggaa ttatcacctc
     4801 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     4861 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     4921 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     4981 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     5041 agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc tgattttttg
     5101 agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc
     5161 ctcgtaaaga attatgtcgt gcgtcaagtt atggcccagc ggtgcccccg cccccttggt
     5221 gtccatcgag gaactggaga accaggagct cgtcggcaaa ggcgggttcg gcacagtgtt
     5281 ccgggcgcaa cataggaagt ggggctacga tgtggcggtc aagatcgtaa actcgaaggc
     5341 gatatccagg gaggtcaagg ccatggcaag tctggataac gaattcgtgc tgcgcctaga
     5401 aggggttatc gagaaggtga actgggacca agatcccaag ccggctctgg tgactaaatt
     5461 catggagaac ggctccttgt cggggctgct gcagtcccag tgccctcggc cctggccgct
     5521 cctttgccgc ctgctgaaag aagtggtgct tgggatgttt tacctgcacg accagaaccc
     5581 ggtgctcctg caccgggacc tcaagccatc caacgtcctg ctggacccag agctgcacgt
     5641 caagctggca gattttggcc tgtccacatt tcagggaggc tcacagtcag ggacagggtc
     5701 cggggagcca gggggcaccc tgggctactt ggccccagaa ctgtttgtta acgtaaaccg
     5761 gaaggcctcc acagccagtg acgtctacag cttcgggatc ctaatgtggg cagtgcttgc
     5821 tggaagagaa gttgagttgc caaccgaacc atcactcgtg tacgaagcag tgtgcaacag
     5881 gcagaaccgg ccttcattgg ctgagctgcc ccaagccggg cctgagactc ccggcttaga
     5941 aggactgaag gagctaatgc agctctgctg gagcagtgag cccaaggaca gaccctcctt
     6001 ccaggaatgc ctaccaaaaa ctgatgaagt cttccagatg gtggagaaca atatgaatgc
     6061 tgctgtctcc acggtaaagg atttcctgtc tcagctcagg agcagcaata ggagattttc
     6121 tatcccagag tcaggccaag gagggacaga aatggatggc tttaggagaa ccatagaaaa
     6181 ccagcactct cgtaatgatg tcatggtttc tgagtggcta aacaaactga atctagagga
     6241 gcctcccagc tctgttccta aaaaatgccc gagccttacc aagaggagca gggcacaaga
     6301 ggagcaggtt ccacaagcct ggacagcagg cacatcttca gattcgatgg cccaacctcc
     6361 ccagactcca gagacctcaa ctttcagaaa ccagatgccc agccctacct caactggaac
     6421 accaagtcct ggaccccgag ggaatcaggg ggctgagaga caaggcatga actggtcctg
     6481 caggaccccg gagccaaatc cagtaacagg gcgaccgctc gttaacatat acaactgctc
     6541 tggggtgcaa gttggagaca acaactactt gactatgcaa cagacaactg ccttgcccac
     6601 atggggcttg gcaccttcgg gcaaggggag gggcttgcag caccccccac cagtaggttc
     6661 gcaagaaggc cctaaagatc ctgaagcctg gagcaggcca cagggttggt ataatcatag
     6721 cgggaaagga ggcggcggaa gcggcggtgg taagatggac aaaaagacta tagtttggtt
     6781 tagaagagac ctaaggattg aggataatcc tgcattagca gcagctgctc acgaaggatc
     6841 tgtttttcct gtcttcattt ggtgtcctga agaagaagga cagttttatc ctggaagagc
     6901 ttcaagatgg tggatgaaac aatcacttgc tcacttatct caatccttga aggctcttgg
     6961 atctgacctc actttaatca aaacccacaa cacgatttca gcgatcttgg attgtatccg
     7021 cgttaccggt gctacaaaag tcgtctttaa ccacctctat gatcctgttt cgttagttcg
     7081 ggaccatacc gtaaaggaga agctggtgga acgtgggatc tctgtgcaaa gctacaatgg
     7141 agatctattg tatgaaccgt gggagatata ctgcgaaaag ggcaaacctt ttacgagttt
     7201 caattcttac tggaagaaat gcttagatat gtcgattgaa tccgttatgc ttcctcctcc
     7261 ttggcggttg atgccaataa ctgcagcggc tgaagcgatt tgggcgtgtt cgattgaaga
     7321 actagggctg gagaatgagg ccgagaaacc gagcaatgcg ttgttaacta gagcttggtc
     7381 tccaggatgg agcaatgctg ataagttact aaatgagttc atcgagaagc agttgataga
     7441 ttatgcaaag aacagcaaga aagttgttgg gaattctact tcactacttt ctccgtatct
     7501 ccatttcggg gaaataagcg tcagacacgt tttccagtgt gcccggatga aacaaattat
     7561 atgggcaaga gataagaaca gtgaaggaga agaaagtgca gatctttttc ttaggggaat
     7621 cggtttaaga gagtattctc ggtatatatg tttcaacttc ccgtttactc acgagcaatc
     7681 gttgttgagt catcttcggt ttttcccttg ggatgctgat gttgataagt tcaaggcctg
     7741 gagacaaggc aggaccggtt atccgttggt ggatgccgga atgagagagc tttgggctac
     7801 cggatggatg cataacagaa taagagtgat tgtttcaagc tttgctgtga agtttcttct
     7861 ccttccatgg aaatggggaa tgaagtattt ctgggataca cttttggatg ctgatttgga
     7921 atgtgacatc cttggctggc agtatatctc tgggagtatc cccgatggcc acgagcttga
     7981 tcgcttggac aatcccgcgt tacaaggcgc caaatatgac ccagaaggtg agtacataag
     8041 gcaatggctt cccgagcttg cgagattgcc aactgaatgg atccatcatc catgggacgc
     8101 tcctttaacc gtactcaaag cttctggtgt ggaactcgga acaaactatg cgaaacccat
     8161 tgtagacatc gacacagctc gtgagctact agctaaagct atttcaagaa cccgtggagc
     8221 acagatcatg atcggagcag cagcccggga tccaccggtc gccaccatgg tgagcaaggg
     8281 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     8341 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     8401 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     8461 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     8521 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     8581 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     8641 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     8701 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     8761 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     8821 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     8881 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     8941 ccactccacc ggcggcatgg acgagctgta caagtaaaat tcccgtatac accggtgccg
     9001 gcggatccag accacctccc ctgcgagcta agctggacag ccaatgacgg gtaagagagt
     9061 gacatttttc actaacctaa gacaggaggg ccgtcagagc tactgcctaa tccaaagacg
     9121 ggtaaaagtg ataaaaatgt atcactccaa cctaagacag gcgcagcttc cgagggattt
     9181 gagatccaga catgataaga tacattgatg agtttggaca aaccaaaact agaatgcagt
     9241 gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc cttatttgta accattataa
     9301 gctgcaataa acaagttaag tcgataaact ggatctctgc tgtccctgta ataaacccga
     9361 aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt
     9421 atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaggcg
     9481 ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc
     9541 ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga
     9601 taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct
     9661 taaacctacc aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt
     9721 tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt
     9781 cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt
     9841 aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt
     9901 actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca
     9961 aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag
    10021 ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag
    10081 caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt
    10141 cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc
    10201 tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac
    10261 tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc aattgtccct
    10321 catatctcct cctccaggtc tgaagatcag cggccggccg cttgctgtgc ggtggtctta
    10381 cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt tatctctatc
    10441 ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat
    10501 cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct
    10561 aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt
    10621 aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt
    10681 ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc
    10741 aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca
    10801 agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc
    10861 gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
    10921 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
    10981 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
    11041 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
    11101 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
    11161 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
    11221 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
    11281 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
    11341 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
    11401 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
    11461 tcaaggatat cttgtcttct ttgggagtga attagccctt ccaactacta agtttgtagt
    11521 acatatttaa caaatacaat ttctttaaaa tgaaaataat tcagaggaat cacaggttta
    11581 gagtaaatga aaccacaggt aattggcagt ggtaataggg tatggggtgg gaagtttggg
    11641 atgattttgg ttagcttgag ttatccagtt gatccagaca tgataagata cattgatgag
    11701 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
    11761 gctattgctt tatttgtaac cattataagc tgcaataaac aagttgatct cccgatccgt
    11821 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    11881 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    11941 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    12001 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    12061 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    12121 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    12181 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    12241 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    12301 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    12361 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    12421 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    12481 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    12541 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    12601 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    12661 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    12721 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    12781 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    12841 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    12901 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    12961 accccgtaga aaagatcaaa ggatcttc
//
