LOCUS       sequence-412259-       14632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  R6K plasmid with a I-B CAST but no CRISPR array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14632)
  TITLE     Distinct horizontal transfer mechanisms for type I and type V 
            CRISPR-associated transposons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pR6K-GFP-CASTIB
FEATURES             Location/Qualifiers
     source          1..14632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7897..7999
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             8000..8659
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8066..8085)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    8660..8706
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(9086..9103)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9086..9102)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9095..9117)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9311..9330)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9430..9452
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9490..9508)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9576..9680
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9681..9749,9750..10541)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9899..9918)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10950..11306
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            11357..11465
                     /note="incP origin of transfer"
     primer_bind     11706..11723
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        11725..11759
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             11839..11850
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             11857..12570
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 caaatagaac gagaaaatac acgcatttta tctcagttaa aacgctgcac caatactgac
       61 aatttccgaa aatttattgc agagttttgg ggaagggcag ggcaaatagc aattctggaa
      121 gaacattgga tggaattact tccattgact acagggatag gcaattggaa agtggccagg
      181 gacttaactt ttattgcaat ggctagctac ccaaagaata aaataacaga ggtagaggta
      241 aaaccatcac aaatatcgga gctaaatact gaataaactt catatttttt gatttgatta
      301 agaacaaaat attaattgtt taggtaatgg tggaatgttt catctgtttg gtaatatttt
      361 gactagttat ggaaccgccg ctaataatcg tggtgaaaat gaaggtaata ccactacgtt
      421 acaaaaatta atttggaaag gtgaagttca ctctactgtt tcatctgagg caattcgttg
      481 ggcattgcga tattattggc aaaatgctgg ctatcctgtg aatagacgtt gggatgaaaa
      541 tgctcaacca gtacccgatc acatcttgca agatcctaat tttgatgata ttcgcttcat
      601 tgatgatgat gtcttgggat ttatgcaagc ggaagcagca aaagttgaag cagcagttga
      661 atccgaagtt gaaatccaga actcaactca agatgaggac aaaaaacttt ctcaaggtaa
      721 ggagaaagaa actgcaaaac gtaagcgtaa ggaaaaacct aaagggaaaa taactactag
      781 acgaggtgta ttagaagtca ctcgtgcagt ttcaacaatt ccttatgctg gtgatcttac
      841 tttcaatgct atgagtggaa agaaaggacg aacatctctt tattctacag aagttcatgc
      901 tactcgatat caatatggct tcgcattaac acccaatagc ctcaaagata aatctcgcat
      961 taatgctgtc ctcgatagct taatttctat tggagaagtg gcaggaaatc acgctcgttt
     1021 tctctatgat ttttctcctg atagcatcat attacggtgg acacacgatt tctcacctcg
     1081 gttactttac tgttttgaag aagatgaact tagaaatata tcaacaccgg atttagtaag
     1141 gcgggtagaa tctggagata tagaccctaa agaattatgg ataggagggg caatttctag
     1201 cactctggaa gatttagggg caaatgtgtt tcccggagtg aagacaacag ttgaagcatt
     1261 aaaacaggtg attattgaag atttgcagtt gtcgttaagg agttcatgat atgacgacaa
     1321 ttgccttaaa agtagaagtt cccatagctt gttttcggca gtctcgtgct agggaatatg
     1381 gcgaaactta ccctgtacca ccaccatcga cagtatatgg aatgttacta tctcttgttg
     1441 gagaagtgga tagatacaaa cactgcggtg tgaagctggc gatcgcactt ctatctgaac
     1501 cagaaaagtc aactgttatt cgcacctttc accgatttaa gacgaagaat atccatgact
     1561 ctaaaaataa taaacctgat tatcaggagt tattaactaa tatagaattt atagtttggg
     1621 tggatgctgg agtagataaa gctaaaccaa atttagtgga acgtttagca gaggcattga
     1681 ctaacccggc ttctattaat aggtttggtg gattatgttt aggtgaaagt cgggatttag
     1741 tgaatgatgt taccctttta cctaaaaatt actatgcaga atcaatgcga tggttaattc
     1801 gagatgaata tgggttacta actttacctt actgggtaga ccatgtaggt tcgcggggaa
     1861 cgcgatggct acgttacgag attcagaaat gtccagtctt tcaaccacct gaattatctt
     1921 ggacttcagt tcaaagtaat taggaggttt aactttaata aggagatata ccatggttag
     1981 gggtagaagg gaatggactc aagctaaata tgagcgatat ataaaagaag gtcgtggccg
     2041 aggaagtggt aagcactata aaccttggct tacaatccaa gatgttccct ctaaaggacg
     2101 ttcttctaga actcctggtt ggaaaacaaa tcgagtacat catttcttct cagaccatga
     2161 aaaacggcta ttttatttgt ttgagtggtc agatatagtc acagatataa gagagcaatt
     2221 tcctttagac aatctcgatc tagccatgag tattgctaca gacataggta taaagtaccc
     2281 tgtagataca caaagtggca ctcctcacgt tttaacaagc gactttatgc ttacagtcaa
     2341 ccgcgatggg aagcagatgc agattgcccg gactgttaag ccgtcaacag aactggagaa
     2401 aaagcaagtt aatgagaaat tcgagataga aagacaatat tacttagcaa agggtattga
     2461 ctggggaatt atcacagaaa aagaaattcc caggctttta gctgaaaatg ttgagtgggt
     2521 tcattctgct tataggctag aagcaaattc tgaaatggac actgcacaac tgagtgaatt
     2581 gtcaacaatt ttaaaatcta agcttcagga agaagataca actataaata gagtaactac
     2641 tgctttagac aaagaaatga atataaattc cggtacatca ctttatgtat ttaaacattt
     2701 aattgctagg aaagaaatcc ttgtggatct agtagcaata aaattatctg cttgtccgtc
     2761 aactaaagtt attgaaaaaa taatatttta gacccacatg ataggtaaat aatatggtta
     2821 aagatttatt tgtcaatgat ttaatagagt ggattgtata ccaaaacact catatagtcg
     2881 aaagaattat ttggatagat gaaggttaca ttattgcatt tgttatagat attaatgcta
     2941 agaaaggtct acctgaacca aggaaaatat cagatatttt agaagctata actgaaggta
     3001 ttgcttttaa gcagcaacaa gatccttggg caagaattat tcgggatgaa aatttgacag
     3061 ataaggaaaa ggaatacaga gataaagctt gggagataat ttcatcatta attatagaag
     3121 aaccatcaat atattataga aattttagag gatcgatggt taaagaagcg gttgaaaagt
     3181 acaacgaaga aagggataaa gataaattag tagaaaaaac cgtctatggt tatttgagaa
     3241 gattttggca gagaggaaaa actataaatg ctctcatacc tgattttatc aattcatcag
     3301 gtaagggcaa gattcgagga caatcgggca agaagagagg aagaccaaga aaatatgcac
     3361 atgacccaga tattggtgaa ggtgtaaatg ttacagaaga agatagaaag atttttagag
     3421 tatctataag caaattttac aataatccaa aagaaaacta cttaacaaca gcctatgaat
     3481 taatggtgaa agaatattac aaggaaggaa ttagatatga cgagaatggt gttcaaaaat
     3541 ctatattaac cccaactgat aaaataccta caattactca attcaaatac tggtatcaac
     3601 ttgaacagga tgatgttacc aaaactttaa catctcgaaa aggagccaag cgagtagctt
     3661 tagagaatag agcaattttg ggaaactcta agatggaaac tattggccct ggttctagat
     3721 atcaaattga tgccacagtt gctgatgtat atttagtttc aaaatataac cgtgactgga
     3781 taattggtag accagttatc tatgtaatta ttgatgtttt tagtagaatg attacaggtg
     3841 tttatgtcgg tttagaaggc ccatcttgga taggagcaat gatggcacta gccaatgtag
     3901 ctacagataa aaccctgttt tgccaggaat ataacattaa tattactgaa aatgaatggc
     3961 cttgcaatca aatgccagat gcaattttag gagatagggg agaattagca ggtatgacag
     4021 tagaaacatt aatccctaat ctttatgttc gtattgaaaa tgcagcttcc tatagagcag
     4081 attggaaagg gttagtagaa agacactttc gtataattca tgaatatgta aaaccttttt
     4141 tacctggtta tgtggatacg gattttaggc aaagaggtgc aagggattat aggcttgatg
     4201 gcaaacttga tatagatcaa tttactgaga ttattattcg tttaattgtt caccataaca
     4261 ataaacctct tactgaatat gaaagagatg aagcaatgat tgctgatgat acccctccta
     4321 tacctagaga gttgtggaag tggggtatcg ccaatcgttc tggcagactc agaacttttt
     4381 ctgaagatat tgtcaaattg aacttgatgc ctactgagaa agcaactatt actgctaaag
     4441 gcatcaaaat gagaggtaag gagatgtact atacctgtga tagtgctaaa aatgagcaat
     4501 ggtttgaaaa agctagaagt aatctccttt ctaagtcaga aaaatcattg attgtatctt
     4561 atgacatcag aaagccaaac ttcatttatc ttccatctag tgatggtaga gattttgaaa
     4621 aatgttttct tattgactta gaagggcggt atgctaataa aaactttcat gatattgaat
     4681 atctgcttgc ttatgaagaa ttgcaaagac agaaaagcca aggtaaacag ctacaagaaa
     4741 aggtagattt aatagctgat attgaaagca tagtagatag agcagaaaaa atgactgatg
     4801 cttctcaata tcataattta agtaagagca agaaagtttc tggaataaga gataataggg
     4861 cttttgaaaa ggctaaacga cgagagaatg aaggatttga attagcaatt acagataatc
     4921 agagcagttt tcaggttgca gagagtacag cagaaacctc cgaacagcca gaatcactac
     4981 agccaaacca tctgaatctt cttaagaaaa agcgacagga gcgaaaacgt ggacagaatt
     5041 gagagtgaac tggttaaaat tcctaatgga gaactagcag ttattgccag atatatagat
     5101 caaaaactgc cagaatacaa cactaatcca ttaattcagg cattacctcc tatcctatca
     5161 gcagcagaat ttatagataa agtagccaga gaaccaaatt ttgatgaaca agagagagag
     5221 ctagaaccac attatagatt tcattgtatt gagaggttat ctagatattt tgaccctcaa
     5281 aataaaactc ttgaattaca aaaagttatt tgtacgctta tcatgacagg ctatctgtca
     5341 aggaaccttt taaaacctga atatgcaaat cgctctaggc aaatttataa tgcaataggt
     5401 aatggaggag gtaaaaattt agaaaactat gttaatcttc ctacatctgc gtccggctta
     5461 actctaattg gcccatcagg tattggaaag actacaaatt tcaaaaatat tcttgagctt
     5521 tatccccaaa taatttttca tcctgattat agtgtttacc aaatagtttg gctgcgggta
     5581 gattgcccta aaactttaaa ggggttatgt atagatactt ttgcaggtat cgataaattg
     5641 ttaggaacta atttattaaa gaaatttaac ccagcacgca catctgaaga ttatctattg
     5701 gctcaacttg cacaaacagc acacactcat catctaggag ttttggtgat tgatgaaata
     5761 caaaatttag caactgcaaa aagaaataga gacgaaatat taaatttttt agtaaagctc
     5821 gataatacta ttggaattcc agttattcgt gtaggtacga atgaagccat tagttttctt
     5881 caaggtaatc ttagaaatgc tagaagaaat actggagaag gaagtagaat gtgggataga
     5941 atggtaaata atgatgaatg gtgttacttt ttagaaggaa tatgggaata ccaatggaca
     6001 aaaacacctg ttcatttctc ggatgaaata aaagaagttt tttatgatga aagccaggga
     6061 attattgata ttgctataaa actttacaaa atggttcaat ggagagcgat ttcaataggt
     6121 gaccaagaaa ttattacggt tgatctgatt cgtcaagtag ctaaagaagg attgtattta
     6181 gtaaagccaa tgctagatgc aattaggtta ggggacagag agtggatgat taaatataag
     6241 gatattgctc ctctcgatat tacggaatat cacaatagat gtgtatctga gattgatgct
     6301 aaagatttga cagagattaa gagattagct aaaaagcagc agaatcagca gaaagtatct
     6361 ccgaagctca attatgtaat tagtgagctg ttgaaattgg aagtagaatc tggtaaagca
     6421 aaggaatgtg cagaacaaat tgtttatagt catgaagatc cagacattcc aattccatct
     6481 ttagttaata aggcatatgc tcttgctttg caaggatgtc aagtaaatga gagtcttgct
     6541 aaaacaagta ccaggaaagc taaattaaag acaaagtacc aagagaatga tattcggcga
     6601 attgtcgaga atgccaagaa agacaaaaaa tctgcatacg aggatttgaa attagcaggt
     6661 ataatcaaaa atccagttga agattttatc aatatttagc ttctaaatgc ttagtttttt
     6721 cccaactctg tatcccgatg aacttttata tagcacttta gctagatacc atattcggag
     6781 tggaaataaa agtttcaagc aaactgactt agagctattc ggttacagtt cccaacaaat
     6841 atgcaaaatt accttaacta ataatttaaa ttatttagtg aaaaacttac ctttacgctc
     6901 ccagcaaaaa gttgagaatt tactacaaaa tcatacttta tattcctttt atgcaacttt
     6961 attaatgccg caggaggctt ggctgctgaa aaactcaatg agtaataaat tcagtgggtc
     7021 tatactagat tttgctaaag tggctaataa ttctagtgat gattcaaaaa aatttttaaa
     7081 attttgccct gaatgtttag agaaagatac ccaaaagtat ggtgaacctt actggcatag
     7141 aatacatcaa gtccctggca ttttagtttg tccaattcat cgtgttgttt tacacaatag
     7201 ctgtatagaa gttgaatcga aaggcataca ttatcatgct gccagcatag ataattgttt
     7261 attagcaaat aatgtgaaaa aattgacaaa taaaactgta gaaacacttt caatactggc
     7321 acatgatatt aattggctaa ttcatagcag ttttgccttt caaggtttgg cttggcttcg
     7381 cgctcagtac cagcgttact tattggataa aggactgcta aaaacctttg ctggtggcaa
     7441 gtttaagttt aatgattgtg gttttgccca gcttatattt gagttttatg gtcaagaatc
     7501 cttagaagcc ctcaatccaa agctgaccca aaatcaaggt aaatattttt ctcactgctt
     7561 gtttggatgc gacttaaacc cagcaattga ccgcataact catatcttaa tgataaaatt
     7621 tttagcaaat tctatagaag agttctttca gtcataactt aagtcgaaca gaaagtaatc
     7681 gtattgtaca ccatcgtggc cggatcttgc ggcccctcgg cttgaacgaa ttgttagaca
     7741 aacgtgtgga ggaataattc ggttcaacaa tatataattc ggttcaacaa ttaataaaac
     7801 gattgaacaa taacctctaa aggagtacga ttgtaccaag taaaagtgcg atcgcttcca
     7861 gaggttattt ttatggttag gggtagaagg gaatggtgat cggcacgtaa gaggttccaa
     7921 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttgt cgagattttc
     7981 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     8041 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     8101 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     8161 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattacg
     8221 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     8281 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     8341 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     8401 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     8461 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     8521 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     8581 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     8641 gtggcagggc ggggcgtaaa agttcctatt ccgaagttcc tattctctag aaagtatagg
     8701 aacttcgaag cagctccagc ctacacaatc gctcaagacg tgtaatgctg caatctgcat
     8761 gcaagcttgg cactggccac gcaccgggat atttacgttt tgtaatcacg aatagattct
     8821 tgattgcaag atgaattgcg atcgcatctt tgtcgcacag cagaactttc tcaaataatt
     8881 ttgaacttat ggaattactc aaaaccttta tcagtaatgg attcaaacaa ttgtttaacc
     8941 gttttatttg agatttttac taaaaatcaa gtttaccata gaggttaagc tgaaattgtt
     9001 caatcgtttt attttatcga caacggcaga ttaaatattc gaactgtaat aattgtttaa
     9061 ccgaattctt tgtctacata tgatcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     9121 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     9181 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     9241 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     9301 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     9361 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     9421 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     9481 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     9541 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9601 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9661 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9721 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9781 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     9841 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     9901 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     9961 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10021 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10081 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10141 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10201 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    10261 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    10321 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    10381 gagccggtga gcgtggctct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10441 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    10501 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10561 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    10621 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10681 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10741 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10801 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    10861 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    10921 tcgctctgct aatcctgtta ccagccggtt gtcagccgtt aagtgttcct gtgtcactca
    10981 aaattgcttt gagaggctct aagggcttct cagtgcgtta catccctggc ttgttgtcca
    11041 caaccgttaa accttaaaag ctttaaaagc cttatatatt cttttttttc ttataaaact
    11101 taaaacctta gaggctattt aagttgctga tttatattaa ttttattgtt caaacatgag
    11161 agcttagtac gtgaaacatg agagcttagt acgttagcca tgagagctta gtacgttagc
    11221 catgagggtt tagttcgtta aacatgagag cttagtacgt taaacttgag agcttagtac
    11281 gtgaaacatg agagcttagt acgtactatc aacaggttga actgcccatg ttctttcctg
    11341 cgttatcaga gcttatcggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
    11401 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
    11461 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
    11521 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
    11581 agggttatgc agcggaaagt ataccttaaa tgttctttcc tgcgttatcc cctgattctg
    11641 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    11701 agcgcagcga gtcagtgagc gaggttgaca gctagctcag tcctaggtat aatactagcg
    11761 tcgacgtgga gatataccaa acgagagacc catcgcggca actcaagaaa cggcaggttc
    11821 ggatcttagc tactagagaa agaggagaaa tactagatgc gtaaaggcga agagctgttc
    11881 actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc
    11941 gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt
    12001 actactggta aactgccggt tccttggccg actctggtaa cgacgctgac ttatggtgtt
    12061 cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg
    12121 ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg
    12181 cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt
    12241 gactttaaag aggacggcaa tatcctgggc cataagctgg aatacaattt taacagccac
    12301 aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc
    12361 cacaacgtgg aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc
    12421 ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct
    12481 aaagatccga acgagaaacg cgatcatatg gttctgctgg agttcgtaac cgcagcgggc
    12541 atcacgcatg gtatggatga actgtacaaa tgaccaggca tggtctccta tggatccgaa
    12601 ttcgagcgaa ggagatatac atccctccat cttagtatat tagttaagta taagaaggag
    12661 atatacatat gattattgat agtaacttag ttacgcagct tccctcagaa tcatcttcaa
    12721 agccaagaga gcttttaatg tctcagtttc catatatgga tttaactttt agccttacgg
    12781 gtgagacatt acctttcgac cacggctatg aattattttc tgcgatcgcc cactttgaac
    12841 ccaaactaca tacattagac acgttgggaa ttcagaccat tgcaggtatt cccaaggatg
    12901 gtgtaattaa tctgacccaa aattctaggc ttcgtattcg gataccagta aatcaggtac
    12961 gtttggttta tccattggcg ggtaagtcac tgagaattgg caagcataca attcgtttgg
    13021 gtattcctga catctgtttg cttcaacctg cggagaaact gcgatcgcgc attgtggtaa
    13081 taagaggcta tgaagaacct gaaagttttt tagtcgtagc gcagcgtcag ctagaacaac
    13141 tgggtatcca agcaattgct agcattccca ccaaagccaa tggcaaacca atacgtagga
    13201 ctatcaaaat taagcgattt acagtcgtgg gttttggatt agaagttata aatttaagcg
    13261 atgaagactc actgaggttg caaatacatg gtgtaggagg aaagcaaaag atgggttgtg
    13321 gactcttcat gcctattaag gaaaggcaat agcaattact cgaaagcaga tgatgaagag
    13381 agcagataac cccaaaattc agcttaatct cggcgatcct agtatgactt tattgcatcg
    13441 tgctggggtt gcaggacttt ggatgacgct caaaaaactg gaaaaaatct atccaactcc
    13501 tgctgaacgt attggtaatt taacttggtt gctaacccct cactatatca atcttgattg
    13561 ggaaggacag gatttcacag ttttagactg gttgctgaag caatcatttc aaatcagcga
    13621 tgagggttta atttccctga cagggttaga ttctcaaaac atggatattc aaaatcaaat
    13681 aattatccat caaggtatca cagccacttt tacacaacat aataaatttt ttaagtctgc
    13741 gggtcaacaa tctaagcaac taattattga tggtattcag gttagggttg attataagaa
    13801 agctgcttcc tatgctcatc aacattttgc taaacattta tgtgaccagt acggacaatt
    13861 gctaaaagag ccaattggta tcaggggatg gctctatcca ggagcagtag ttcgccatta
    13921 tgcttttaaa gaacaaacag catttgaaga aaaagcagaa tatgctctag cgttgttgtt
    13981 cgctcctatg gcttgtcaat attttgtgct gcgatcgcac accgaaccat cacatacaaa
    14041 ctatgttttg gttattcctg aagtaattga tttagaactt tatgcacatt ttttgtggtg
    14101 tttgggtaac ttagattata aacattttca cgtatctagc ttaggagatg ctggattaaa
    14161 atttcttaat tataaaacaa cacaaatgcc tacttccaat tttttaaaac gatgtcaaat
    14221 aatctttttt agtacaaaag cttggtcaga acagcaaaaa actcgtgcag aaatagcaat
    14281 catagaatta aattcttcag ttaattatct ttataagctt agttgtattt gttttccaga
    14341 atgtcaaatt gttttatata aaaatcaaca ttatttgctt ccaagtttaa taaaaggaat
    14401 aatcgccaat aatctagcaa tgggtcggtc ttggtgggct aattggattt catttattaa
    14461 aaataaaaaa tcatttaaac aatttactga tgagtataaa ggaattaata agatgattca
    14521 ggactcagaa tgggatatag aagctcaaaa gctatttatt caagcttgtc atgaagcatt
    14581 aagaaaaata tatgccaaaa tttatagcag aacaaaagaa ggtgaatacg ct
//
