LOCUS       sequence-412263-       13934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  R6K plasmid with a I-B CAST targeting lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13934)
  TITLE     Distinct horizontal transfer mechanisms for type I and type V 
            CRISPR-associated transposons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pR6K-crRNA-CASTIB
FEATURES             Location/Qualifiers
     source          1..13934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      316..672
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            723..831
                     /note="incP origin of transfer"
     primer_bind     1072..1089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1091..1125
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     promoter        11197..11299
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             11300..11959
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(11366..11385)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    11960..12006
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(12386..12403)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12386..12402)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12395..12417)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12611..12630)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12730..12752
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12790..12808)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12876..12980
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12981..13049,13050..13841)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13199..13218)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
       61 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      121 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      181 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      241 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      301 ctgttaccag ccggttgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga
      361 ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct
      421 taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg
      481 ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga
      541 aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt
      601 tcgttaaaca tgagagctta gtacgttaaa cttgagagct tagtacgtga aacatgagag
      661 cttagtacgt actatcaaca ggttgaactg cccatgttct ttcctgcgtt atcagagctt
      721 atcggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
      781 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
      841 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
      901 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
      961 gaaagtatac cttaaatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     1021 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     1081 gtgagcgagg ttgacagcta gctcagtcct aggtataata ctagcgtcga cgtggagata
     1141 taccaaacgg tgctttaaca ttagatgtcg ttaggcgttg agcattcacc gccgaaaggc
     1201 gcggtgccgc tggcgacctg cgtgctttaa cattagatgt cgttaggcgt tgagcatatg
     1261 gatccgaatt cgagcgaagg agatatacat ccctccatct tagtatatta gttaagtata
     1321 agaaggagat atacatatga ttattgatag taacttagtt acgcagcttc cctcagaatc
     1381 atcttcaaag ccaagagagc ttttaatgtc tcagtttcca tatatggatt taacttttag
     1441 ccttacgggt gagacattac ctttcgacca cggctatgaa ttattttctg cgatcgccca
     1501 ctttgaaccc aaactacata cattagacac gttgggaatt cagaccattg caggtattcc
     1561 caaggatggt gtaattaatc tgacccaaaa ttctaggctt cgtattcgga taccagtaaa
     1621 tcaggtacgt ttggtttatc cattggcggg taagtcactg agaattggca agcatacaat
     1681 tcgtttgggt attcctgaca tctgtttgct tcaacctgcg gagaaactgc gatcgcgcat
     1741 tgtggtaata agaggctatg aagaacctga aagtttttta gtcgtagcgc agcgtcagct
     1801 agaacaactg ggtatccaag caattgctag cattcccacc aaagccaatg gcaaaccaat
     1861 acgtaggact atcaaaatta agcgatttac agtcgtgggt tttggattag aagttataaa
     1921 tttaagcgat gaagactcac tgaggttgca aatacatggt gtaggaggaa agcaaaagat
     1981 gggttgtgga ctcttcatgc ctattaagga aaggcaatag caattactcg aaagcagatg
     2041 atgaagagag cagataaccc caaaattcag cttaatctcg gcgatcctag tatgacttta
     2101 ttgcatcgtg ctggggttgc aggactttgg atgacgctca aaaaactgga aaaaatctat
     2161 ccaactcctg ctgaacgtat tggtaattta acttggttgc taacccctca ctatatcaat
     2221 cttgattggg aaggacagga tttcacagtt ttagactggt tgctgaagca atcatttcaa
     2281 atcagcgatg agggtttaat ttccctgaca gggttagatt ctcaaaacat ggatattcaa
     2341 aatcaaataa ttatccatca aggtatcaca gccactttta cacaacataa taaatttttt
     2401 aagtctgcgg gtcaacaatc taagcaacta attattgatg gtattcaggt tagggttgat
     2461 tataagaaag ctgcttccta tgctcatcaa cattttgcta aacatttatg tgaccagtac
     2521 ggacaattgc taaaagagcc aattggtatc aggggatggc tctatccagg agcagtagtt
     2581 cgccattatg cttttaaaga acaaacagca tttgaagaaa aagcagaata tgctctagcg
     2641 ttgttgttcg ctcctatggc ttgtcaatat tttgtgctgc gatcgcacac cgaaccatca
     2701 catacaaact atgttttggt tattcctgaa gtaattgatt tagaacttta tgcacatttt
     2761 ttgtggtgtt tgggtaactt agattataaa cattttcacg tatctagctt aggagatgct
     2821 ggattaaaat ttcttaatta taaaacaaca caaatgccta cttccaattt tttaaaacga
     2881 tgtcaaataa tcttttttag tacaaaagct tggtcagaac agcaaaaaac tcgtgcagaa
     2941 atagcaatca tagaattaaa ttcttcagtt aattatcttt ataagcttag ttgtatttgt
     3001 tttccagaat gtcaaattgt tttatataaa aatcaacatt atttgcttcc aagtttaata
     3061 aaaggaataa tcgccaataa tctagcaatg ggtcggtctt ggtgggctaa ttggatttca
     3121 tttattaaaa ataaaaaatc atttaaacaa tttactgatg agtataaagg aattaataag
     3181 atgattcagg actcagaatg ggatatagaa gctcaaaagc tatttattca agcttgtcat
     3241 gaagcattaa gaaaaatata tgccaaaatt tatagcagaa caaaagaagg tgaatacgct
     3301 caaatagaac gagaaaatac acgcatttta tctcagttaa aacgctgcac caatactgac
     3361 aatttccgaa aatttattgc agagttttgg ggaagggcag ggcaaatagc aattctggaa
     3421 gaacattgga tggaattact tccattgact acagggatag gcaattggaa agtggccagg
     3481 gacttaactt ttattgcaat ggctagctac ccaaagaata aaataacaga ggtagaggta
     3541 aaaccatcac aaatatcgga gctaaatact gaataaactt catatttttt gatttgatta
     3601 agaacaaaat attaattgtt taggtaatgg tggaatgttt catctgtttg gtaatatttt
     3661 gactagttat ggaaccgccg ctaataatcg tggtgaaaat gaaggtaata ccactacgtt
     3721 acaaaaatta atttggaaag gtgaagttca ctctactgtt tcatctgagg caattcgttg
     3781 ggcattgcga tattattggc aaaatgctgg ctatcctgtg aatagacgtt gggatgaaaa
     3841 tgctcaacca gtacccgatc acatcttgca agatcctaat tttgatgata ttcgcttcat
     3901 tgatgatgat gtcttgggat ttatgcaagc ggaagcagca aaagttgaag cagcagttga
     3961 atccgaagtt gaaatccaga actcaactca agatgaggac aaaaaacttt ctcaaggtaa
     4021 ggagaaagaa actgcaaaac gtaagcgtaa ggaaaaacct aaagggaaaa taactactag
     4081 acgaggtgta ttagaagtca ctcgtgcagt ttcaacaatt ccttatgctg gtgatcttac
     4141 tttcaatgct atgagtggaa agaaaggacg aacatctctt tattctacag aagttcatgc
     4201 tactcgatat caatatggct tcgcattaac acccaatagc ctcaaagata aatctcgcat
     4261 taatgctgtc ctcgatagct taatttctat tggagaagtg gcaggaaatc acgctcgttt
     4321 tctctatgat ttttctcctg atagcatcat attacggtgg acacacgatt tctcacctcg
     4381 gttactttac tgttttgaag aagatgaact tagaaatata tcaacaccgg atttagtaag
     4441 gcgggtagaa tctggagata tagaccctaa agaattatgg ataggagggg caatttctag
     4501 cactctggaa gatttagggg caaatgtgtt tcccggagtg aagacaacag ttgaagcatt
     4561 aaaacaggtg attattgaag atttgcagtt gtcgttaagg agttcatgat atgacgacaa
     4621 ttgccttaaa agtagaagtt cccatagctt gttttcggca gtctcgtgct agggaatatg
     4681 gcgaaactta ccctgtacca ccaccatcga cagtatatgg aatgttacta tctcttgttg
     4741 gagaagtgga tagatacaaa cactgcggtg tgaagctggc gatcgcactt ctatctgaac
     4801 cagaaaagtc aactgttatt cgcacctttc accgatttaa gacgaagaat atccatgact
     4861 ctaaaaataa taaacctgat tatcaggagt tattaactaa tatagaattt atagtttggg
     4921 tggatgctgg agtagataaa gctaaaccaa atttagtgga acgtttagca gaggcattga
     4981 ctaacccggc ttctattaat aggtttggtg gattatgttt aggtgaaagt cgggatttag
     5041 tgaatgatgt taccctttta cctaaaaatt actatgcaga atcaatgcga tggttaattc
     5101 gagatgaata tgggttacta actttacctt actgggtaga ccatgtaggt tcgcggggaa
     5161 cgcgatggct acgttacgag attcagaaat gtccagtctt tcaaccacct gaattatctt
     5221 ggacttcagt tcaaagtaat taggaggttt aactttaata aggagatata ccatggttag
     5281 gggtagaagg gaatggactc aagctaaata tgagcgatat ataaaagaag gtcgtggccg
     5341 aggaagtggt aagcactata aaccttggct tacaatccaa gatgttccct ctaaaggacg
     5401 ttcttctaga actcctggtt ggaaaacaaa tcgagtacat catttcttct cagaccatga
     5461 aaaacggcta ttttatttgt ttgagtggtc agatatagtc acagatataa gagagcaatt
     5521 tcctttagac aatctcgatc tagccatgag tattgctaca gacataggta taaagtaccc
     5581 tgtagataca caaagtggca ctcctcacgt tttaacaagc gactttatgc ttacagtcaa
     5641 ccgcgatggg aagcagatgc agattgcccg gactgttaag ccgtcaacag aactggagaa
     5701 aaagcaagtt aatgagaaat tcgagataga aagacaatat tacttagcaa agggtattga
     5761 ctggggaatt atcacagaaa aagaaattcc caggctttta gctgaaaatg ttgagtgggt
     5821 tcattctgct tataggctag aagcaaattc tgaaatggac actgcacaac tgagtgaatt
     5881 gtcaacaatt ttaaaatcta agcttcagga agaagataca actataaata gagtaactac
     5941 tgctttagac aaagaaatga atataaattc cggtacatca ctttatgtat ttaaacattt
     6001 aattgctagg aaagaaatcc ttgtggatct agtagcaata aaattatctg cttgtccgtc
     6061 aactaaagtt attgaaaaaa taatatttta gacccacatg ataggtaaat aatatggtta
     6121 aagatttatt tgtcaatgat ttaatagagt ggattgtata ccaaaacact catatagtcg
     6181 aaagaattat ttggatagat gaaggttaca ttattgcatt tgttatagat attaatgcta
     6241 agaaaggtct acctgaacca aggaaaatat cagatatttt agaagctata actgaaggta
     6301 ttgcttttaa gcagcaacaa gatccttggg caagaattat tcgggatgaa aatttgacag
     6361 ataaggaaaa ggaatacaga gataaagctt gggagataat ttcatcatta attatagaag
     6421 aaccatcaat atattataga aattttagag gatcgatggt taaagaagcg gttgaaaagt
     6481 acaacgaaga aagggataaa gataaattag tagaaaaaac cgtctatggt tatttgagaa
     6541 gattttggca gagaggaaaa actataaatg ctctcatacc tgattttatc aattcatcag
     6601 gtaagggcaa gattcgagga caatcgggca agaagagagg aagaccaaga aaatatgcac
     6661 atgacccaga tattggtgaa ggtgtaaatg ttacagaaga agatagaaag atttttagag
     6721 tatctataag caaattttac aataatccaa aagaaaacta cttaacaaca gcctatgaat
     6781 taatggtgaa agaatattac aaggaaggaa ttagatatga cgagaatggt gttcaaaaat
     6841 ctatattaac cccaactgat aaaataccta caattactca attcaaatac tggtatcaac
     6901 ttgaacagga tgatgttacc aaaactttaa catctcgaaa aggagccaag cgagtagctt
     6961 tagagaatag agcaattttg ggaaactcta agatggaaac tattggccct ggttctagat
     7021 atcaaattga tgccacagtt gctgatgtat atttagtttc aaaatataac cgtgactgga
     7081 taattggtag accagttatc tatgtaatta ttgatgtttt tagtagaatg attacaggtg
     7141 tttatgtcgg tttagaaggc ccatcttgga taggagcaat gatggcacta gccaatgtag
     7201 ctacagataa aaccctgttt tgccaggaat ataacattaa tattactgaa aatgaatggc
     7261 cttgcaatca aatgccagat gcaattttag gagatagggg agaattagca ggtatgacag
     7321 tagaaacatt aatccctaat ctttatgttc gtattgaaaa tgcagcttcc tatagagcag
     7381 attggaaagg gttagtagaa agacactttc gtataattca tgaatatgta aaaccttttt
     7441 tacctggtta tgtggatacg gattttaggc aaagaggtgc aagggattat aggcttgatg
     7501 gcaaacttga tatagatcaa tttactgaga ttattattcg tttaattgtt caccataaca
     7561 ataaacctct tactgaatat gaaagagatg aagcaatgat tgctgatgat acccctccta
     7621 tacctagaga gttgtggaag tggggtatcg ccaatcgttc tggcagactc agaacttttt
     7681 ctgaagatat tgtcaaattg aacttgatgc ctactgagaa agcaactatt actgctaaag
     7741 gcatcaaaat gagaggtaag gagatgtact atacctgtga tagtgctaaa aatgagcaat
     7801 ggtttgaaaa agctagaagt aatctccttt ctaagtcaga aaaatcattg attgtatctt
     7861 atgacatcag aaagccaaac ttcatttatc ttccatctag tgatggtaga gattttgaaa
     7921 aatgttttct tattgactta gaagggcggt atgctaataa aaactttcat gatattgaat
     7981 atctgcttgc ttatgaagaa ttgcaaagac agaaaagcca aggtaaacag ctacaagaaa
     8041 aggtagattt aatagctgat attgaaagca tagtagatag agcagaaaaa atgactgatg
     8101 cttctcaata tcataattta agtaagagca agaaagtttc tggaataaga gataataggg
     8161 cttttgaaaa ggctaaacga cgagagaatg aaggatttga attagcaatt acagataatc
     8221 agagcagttt tcaggttgca gagagtacag cagaaacctc cgaacagcca gaatcactac
     8281 agccaaacca tctgaatctt cttaagaaaa agcgacagga gcgaaaacgt ggacagaatt
     8341 gagagtgaac tggttaaaat tcctaatgga gaactagcag ttattgccag atatatagat
     8401 caaaaactgc cagaatacaa cactaatcca ttaattcagg cattacctcc tatcctatca
     8461 gcagcagaat ttatagataa agtagccaga gaaccaaatt ttgatgaaca agagagagag
     8521 ctagaaccac attatagatt tcattgtatt gagaggttat ctagatattt tgaccctcaa
     8581 aataaaactc ttgaattaca aaaagttatt tgtacgctta tcatgacagg ctatctgtca
     8641 aggaaccttt taaaacctga atatgcaaat cgctctaggc aaatttataa tgcaataggt
     8701 aatggaggag gtaaaaattt agaaaactat gttaatcttc ctacatctgc gtccggctta
     8761 actctaattg gcccatcagg tattggaaag actacaaatt tcaaaaatat tcttgagctt
     8821 tatccccaaa taatttttca tcctgattat agtgtttacc aaatagtttg gctgcgggta
     8881 gattgcccta aaactttaaa ggggttatgt atagatactt ttgcaggtat cgataaattg
     8941 ttaggaacta atttattaaa gaaatttaac ccagcacgca catctgaaga ttatctattg
     9001 gctcaacttg cacaaacagc acacactcat catctaggag ttttggtgat tgatgaaata
     9061 caaaatttag caactgcaaa aagaaataga gacgaaatat taaatttttt agtaaagctc
     9121 gataatacta ttggaattcc agttattcgt gtaggtacga atgaagccat tagttttctt
     9181 caaggtaatc ttagaaatgc tagaagaaat actggagaag gaagtagaat gtgggataga
     9241 atggtaaata atgatgaatg gtgttacttt ttagaaggaa tatgggaata ccaatggaca
     9301 aaaacacctg ttcatttctc ggatgaaata aaagaagttt tttatgatga aagccaggga
     9361 attattgata ttgctataaa actttacaaa atggttcaat ggagagcgat ttcaataggt
     9421 gaccaagaaa ttattacggt tgatctgatt cgtcaagtag ctaaagaagg attgtattta
     9481 gtaaagccaa tgctagatgc aattaggtta ggggacagag agtggatgat taaatataag
     9541 gatattgctc ctctcgatat tacggaatat cacaatagat gtgtatctga gattgatgct
     9601 aaagatttga cagagattaa gagattagct aaaaagcagc agaatcagca gaaagtatct
     9661 ccgaagctca attatgtaat tagtgagctg ttgaaattgg aagtagaatc tggtaaagca
     9721 aaggaatgtg cagaacaaat tgtttatagt catgaagatc cagacattcc aattccatct
     9781 ttagttaata aggcatatgc tcttgctttg caaggatgtc aagtaaatga gagtcttgct
     9841 aaaacaagta ccaggaaagc taaattaaag acaaagtacc aagagaatga tattcggcga
     9901 attgtcgaga atgccaagaa agacaaaaaa tctgcatacg aggatttgaa attagcaggt
     9961 ataatcaaaa atccagttga agattttatc aatatttagc ttctaaatgc ttagtttttt
    10021 cccaactctg tatcccgatg aacttttata tagcacttta gctagatacc atattcggag
    10081 tggaaataaa agtttcaagc aaactgactt agagctattc ggttacagtt cccaacaaat
    10141 atgcaaaatt accttaacta ataatttaaa ttatttagtg aaaaacttac ctttacgctc
    10201 ccagcaaaaa gttgagaatt tactacaaaa tcatacttta tattcctttt atgcaacttt
    10261 attaatgccg caggaggctt ggctgctgaa aaactcaatg agtaataaat tcagtgggtc
    10321 tatactagat tttgctaaag tggctaataa ttctagtgat gattcaaaaa aatttttaaa
    10381 attttgccct gaatgtttag agaaagatac ccaaaagtat ggtgaacctt actggcatag
    10441 aatacatcaa gtccctggca ttttagtttg tccaattcat cgtgttgttt tacacaatag
    10501 ctgtatagaa gttgaatcga aaggcataca ttatcatgct gccagcatag ataattgttt
    10561 attagcaaat aatgtgaaaa aattgacaaa taaaactgta gaaacacttt caatactggc
    10621 acatgatatt aattggctaa ttcatagcag ttttgccttt caaggtttgg cttggcttcg
    10681 cgctcagtac cagcgttact tattggataa aggactgcta aaaacctttg ctggtggcaa
    10741 gtttaagttt aatgattgtg gttttgccca gcttatattt gagttttatg gtcaagaatc
    10801 cttagaagcc ctcaatccaa agctgaccca aaatcaaggt aaatattttt ctcactgctt
    10861 gtttggatgc gacttaaacc cagcaattga ccgcataact catatcttaa tgataaaatt
    10921 tttagcaaat tctatagaag agttctttca gtcataactt aagtcgaaca gaaagtaatc
    10981 gtattgtaca ccatcgtggc cggatcttgc ggcccctcgg cttgaacgaa ttgttagaca
    11041 aacgtgtgga ggaataattc ggttcaacaa tatataattc ggttcaacaa ttaataaaac
    11101 gattgaacaa taacctctaa aggagtacga ttgtaccaag taaaagtgcg atcgcttcca
    11161 gaggttattt ttatggttag gggtagaagg gaatggtgat cggcacgtaa gaggttccaa
    11221 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttgt cgagattttc
    11281 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
    11341 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
    11401 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
    11461 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattacg
    11521 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
    11581 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
    11641 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
    11701 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
    11761 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
    11821 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
    11881 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
    11941 gtggcagggc ggggcgtaaa agttcctatt ccgaagttcc tattctctag aaagtatagg
    12001 aacttcgaag cagctccagc ctacacaatc gctcaagacg tgtaatgctg caatctgcat
    12061 gcaagcttgg cactggccac gcaccgggat atttacgttt tgtaatcacg aatagattct
    12121 tgattgcaag atgaattgcg atcgcatctt tgtcgcacag cagaactttc tcaaataatt
    12181 ttgaacttat ggaattactc aaaaccttta tcagtaatgg attcaaacaa ttgtttaacc
    12241 gttttatttg agatttttac taaaaatcaa gtttaccata gaggttaagc tgaaattgtt
    12301 caatcgtttt attttatcga caacggcaga ttaaatattc gaactgtaat aattgtttaa
    12361 ccgaattctt tgtctacata tgatcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    12421 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    12481 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    12541 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
    12601 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
    12661 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
    12721 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
    12781 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
    12841 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    12901 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    12961 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    13021 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    13081 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    13141 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    13201 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    13261 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    13321 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    13381 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    13441 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    13501 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    13561 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    13621 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    13681 gagccggtga gcgtggctct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    13741 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    13801 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    13861 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    13921 tcctttttga taat
//
