LOCUS       sequence-412281-        9731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human ETS1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Henriques-Oliveira L, Altman AR, Kurochkin I, Ascic E, Halitzki E, 
            Matei A, Pertiga-Cabral D, Ulmert I, Holst S, Nair MS, Cunha PP, 
            Park S, Vergani S, Kharas MG, Yuan J, Lahl K, Rosa FF, Pires CF, 
            Pereira C-F
  TITLE     Anchored screening identifies transcription factor blueprints 
            underlying dendritic cell diversity and subset-specific anti-tumor 
            immunity
  JOURNAL   Immunity. 58, 1-20. October 2025.
REFERENCE   2  (bases 1 to 9731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            58, 1-20. October 2025."
COMMENT     Sequence Label: pFUW-TetO-ETS1
FEATURES             Location/Qualifiers
     source          1..9731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4212..4800
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4265..4285)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4683..4694)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4803..4819)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4804..4820)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5325..5505
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5531..5548)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5537..5761
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5807..6235
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5894..5913)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6104..6125
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6244..6264)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6249..6578
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6429..6564
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6491..6510
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6626..6673
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6692..7066
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7196..7317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7233..7252)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7287..7306
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7366..7382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7366..7382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7379..7401)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7390..7406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7414..7420,7421..7438,7439..7444))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7459..7480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7597..7614)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7768..8356)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7848..7867)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8527..9318,9319..9387))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9150..9169
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9388..9492)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9567..9586)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagagctag catgaaggcg gccgtcgatc
     2881 tcaagccgac tctcaccatc atcaagacgg aaaaagtcga tctggagctt ttcccctccc
     2941 cggatatgga atgtgcagat gtcccactat taactccaag cagcaaagaa atgatgtctc
     3001 aagcattaaa agctactttc agtggtttca ctaaagaaca gcaacgactg gggatcccaa
     3061 aagacccccg gcagtggaca gaaacccatg ttcgggactg ggtgatgtgg gctgtgaatg
     3121 aattcagcct gaaaggtgta gacttccaga agttctgtat gaatggagca gccctctgcg
     3181 ccctgggtaa agactgcttt ctcgagctgg ccccagactt tgttggggac atcttatggg
     3241 aacatctaga gatcctgcag aaagaggatg tgaaaccata tcaagttaat ggagtcaacc
     3301 cagcctatcc agaatcccgc tatacctcgg attacttcat tagctatggt attgagcatg
     3361 cccagtgtgt tccaccatcg gagttctcag agcccagctt catcacagag tcctatcaga
     3421 cgctccatcc catcagctcg gaagagctcc tctccctcaa gtatgagaat gactacccct
     3481 cggtcattct ccgagaccct ctccagacag acaccttgca gaatgactac tttgctatca
     3541 aacaagaagt cgtcacccca gacaacatgt gcatggggag gaccagtcgt ggtaaactcg
     3601 ggggccagga ctcttttgaa agcatagaga gctacgatag ttgtgatcgc ctcacccagt
     3661 cctggagcag ccagtcatct ttcaacagcc tgcagcgtgt tccctcctat gacagcttcg
     3721 actcagagga ctatccggct gccctgccca accacaagcc caagggcacc ttcaaggact
     3781 atgtgcggga ccgtgctgac ctcaataagg acaagcctgt cattcctgct gctgccctag
     3841 ctggctacac aggcagtgga ccaatccagc tatggcagtt tcttctggaa ttactcactg
     3901 ataaatcctg tcagtctttt atcagctgga caggagatgg ctgggaattc aaactttctg
     3961 acccagatga ggtggccagg agatggggaa agaggaaaaa caaacctaag atgaattatg
     4021 agaaactgag ccgtggccta cgctactatt acgacaaaaa catcatccac aagacagcgg
     4081 ggaaacgcta cgtgtaccgc tttgtgtgtg acctgcagag cctgctgggg tacacccctg
     4141 aggagctgca cgccatgctg gacgtcaagc cagatgccga cgagtgattc gaagatatca
     4201 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4261 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4321 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4381 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4441 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4501 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4561 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     4621 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4681 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4741 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4801 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     4861 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     4921 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4981 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     5041 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     5101 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     5161 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     5221 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     5281 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     5341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5461 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     5521 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5581 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5641 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5701 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5761 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     5821 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5881 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5941 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6001 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6061 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6121 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6181 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     6241 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6301 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6421 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6481 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6541 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     6601 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     6661 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     6721 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     6781 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     6841 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     6901 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     6961 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     7021 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     7081 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     7141 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     7201 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7261 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7321 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7381 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7441 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7501 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7561 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7621 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7681 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7741 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7801 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7861 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7921 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7981 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8041 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8101 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8161 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8221 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8281 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8341 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8401 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8461 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8521 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8581 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8641 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8701 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8761 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8821 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8881 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8941 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9001 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9061 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9121 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9181 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9241 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9301 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9361 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9421 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9481 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     9541 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9601 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     9661 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     9721 gcgctgcttc g
//
