LOCUS       sequence-412349-       10527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting 
            murine cGAS gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, 
            Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, 
            Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis 
            JE, Gupta GP
  TITLE     MRE11 liberates cGAS from nucleosome sequestration during 
            tumorigenesis.
  JOURNAL   Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6.
  PUBMED    38200309
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Jan 10. doi: 10.1038/s41586-023-06889-6."
COMMENT     Sequence Label: Lenti-sgRNA-Cre-GpNLuc-sgmcGAS
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(93..140)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(188..517)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      202..337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(256..275)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     502..522
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(531..959)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(641..662)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     853..872
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1005..1229
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1218..1235
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1261..1494
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1566..2154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1672..1683
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2081..2101
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2170..2685)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(join(2701..3411,3412..3414,3415..3417))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2701..3411,3412..3414,3415..3417))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2743..2764)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3092..3111
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(3418..3474)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3475..4500)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLQ
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             complement(4501..4521)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(4547..4758)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(4820..4895)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(4917..5165)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(4975..4994)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(5145..5165)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    5216..5333
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5631..5675)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5860..6093
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6586..6711
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6758..6938
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6956..7154)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6981..7001)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7156..7535
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7707..7726
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7801..7905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7906..7974,7975..8766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8124..8143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8937..9525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9426..9445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9679..9696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9813..9834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9849..9854,9855..9872,9873..9879)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9887..9903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9892..9914
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9911..9927
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9911..9927
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9976..10097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9987..10006)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10041..10060
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(10227..10527,1..74))
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg
       61 gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat
      121 gccgatgatt aattgtcaac acgtgctgat cagatccgaa aatggatata caagctcccg
      181 ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
      241 cagaggcaga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
      301 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
      361 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
      421 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
      481 gggagcctgg ggactttcca caccctaact gacacacatt ccacagaatt aattcgcgtt
      541 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
      601 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
      661 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
      721 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
      781 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
      841 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
      901 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtg
      961 gggatacccc ctagagcccc agctggttct ttccgcctca gaagccatag agcccaccgc
     1021 atccccagca tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc
     1081 accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta
     1141 ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac
     1201 aacagatggc tggcaactag aaggcacagt cgaggctgat cagcgggttt aaacgggccc
     1261 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     1321 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     1381 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     1441 cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc
     1501 ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag
     1561 tcgacgcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg
     1621 cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc
     1681 cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag
     1741 ccaaggaaag gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg
     1801 agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag
     1861 ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg
     1921 ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc
     1981 ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga
     2041 agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac
     2101 atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt gattgtcgac
     2161 ttaacgcgtt tacgccagaa tgcgttcgca cagccgccag ccggtcactc cgttgatggt
     2221 tactcggaac agcagggagc cgtcggggtt gatcaggcgc tcgtcgataa ttttgttgcc
     2281 gttccacagg gtccctgtta cagtgatctt tttgccgtcg aacacggcga tgccttcata
     2341 cggccgtccg aaatagtcga tcatgttcgg cgtaaccccg tcgattacca gtgtgccata
     2401 gtgcaggatc accttaaagt gatgatcatc cacagggtac accaccttaa aaattttttc
     2461 gatctggccc atttggtcgc cgctcagacc ttcatacggg atgatgacat ggatgtcgat
     2521 cttcagccca ttttcaccgc tcaggacaat cctttggatc ggagttacgg acaccccgag
     2581 attctgaaac aaactggaca cacctccctg ttcaaggact tggtccaggt tgtagccggc
     2641 tgtctgtcgc cagtccccaa cgaaatcttc gagtgtgaag accatacctc cggagatatc
     2701 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     2761 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
     2821 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     2881 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
     2941 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     3001 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     3061 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     3121 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     3181 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
     3241 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
     3301 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     3361 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatcgg
     3421 tccaggattc tcttcgacat ctccggcttg tttcagcaga gagaagtttg ttgcatcgcc
     3481 atcttccagc aggcgcacca ttgcccctgt ttcactatcc aggttacgga tatagttcat
     3541 gacaatattt acattggtcc agccaccagc ttgcatgatc tccggtattg aaactccagc
     3601 gcgggccata tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg ccaggtatct
     3661 ctgaccagag tcatccttag cgccgtaaat caatcgatga gttgcttcaa aaatcccttc
     3721 cagggcgcga gttgatagct ggctggtggc agatggcgcg gcaacaccat tttttctgac
     3781 ccggcaaaac aggtagttat tcggatcatc agctacacca gatacggaaa tccatcgctc
     3841 gaccagttta gttaccccca ggctaagtgc cttctctaca cctgcggtgc taaccagcgt
     3901 tttcgttctg ccaatatgga ttaacattct cccaccgtca gtacgtgaga tatctttaac
     3961 cctgatcctg gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc ccagaaatgc
     4021 cagattacgt atatcctggc agcgatcgct attttccatg agtgaacgaa cctggtcgaa
     4081 atcagtgcgt tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa cgttttcttt
     4141 tcggatccgc cgcataacca gtgaaacagc attgctgtca cttggtcgtg gcagcccgga
     4201 ccgacgatga agcatgttta gctggcccaa atgttgctgg atagttttta ctgccagacc
     4261 gcgcgcctga agatatagaa gataatcgcg aacatcttca ggttctgcgg gaaaccattt
     4321 ccggttattc aacttgcacc atgccgccca cgaccggcaa acggactgaa gcattttcca
     4381 ggtatgctca gaaaacgcct ggcgatccct gaacatgtcc atcaggttct tgcgaacctc
     4441 atcactcgtt gcatcgaccg gtaatgcagg caaattttgg tgtacggtca gtaaattgga
     4501 caccttcctc ttcttcttgg gcatggtaga tcctctagaa ccggtcctgt gttctggcgg
     4561 caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc
     4621 caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc
     4681 accttctcta ggcaccggat caattgccga cccctccccc caacttctcg gggactgtgg
     4741 gcgatgtgcg ctctgcccac tgacgggcac cggagccaat tcccactcct ttcaagacct
     4801 agctagcgaa ttcaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta
     4861 gccttatttt aacttgctat ttctagctct aaaacatcta agacgagagc cgtttcggtg
     4921 tttcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca agttacggta
     4981 agcatatgat agtccatttt aaaacataat tttaaaactg caaactaccc aagaaattat
     5041 tactttctac gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa attaattcta
     5101 attatctctc taacagcctt gtatcgtata tgcaaatatg aaggaatcat gggaaatagg
     5161 ccctcggtac cttaattaac caaactggat ctctgctgtc cctgtaataa acccgaaaat
     5221 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
     5281 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga
     5341 tgaatactgc catttgtctg cagaattggc gcacgcagtg ccgatccgtt cactaatcga
     5401 atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt
     5461 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
     5521 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
     5581 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
     5641 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
     5701 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
     5761 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
     5821 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
     5881 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
     5941 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
     6001 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
     6061 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
     6121 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
     6181 tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc
     6241 agcggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa
     6301 agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt
     6361 attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag
     6421 tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag
     6481 ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa
     6541 ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc
     6601 atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc
     6661 ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg
     6721 tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac
     6781 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     6841 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     6901 agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa acgcgctgct
     6961 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     7021 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     7081 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     7141 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     7201 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     7261 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     7321 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     7381 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     7441 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     7501 tgattactat taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc
     7561 gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc
     7621 ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc
     7681 agggagcaga tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7741 taggggatcg ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg
     7801 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7861 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7921 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7981 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8041 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8101 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8161 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8221 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8281 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8341 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8401 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8461 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8521 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8581 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8641 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8701 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8761 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8821 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8881 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8941 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9001 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9061 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9121 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9181 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9241 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9301 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9361 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9421 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9481 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9541 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9601 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     9661 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     9721 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     9781 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     9841 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9901 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     9961 ggtatacaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
    10021 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
    10081 gctgcaataa acaagttggg gtgggcgaag aactccagca tgagatcccc gcgctggagg
    10141 atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg
    10201 gtggaatcga aatctcgtag cacgtgtcag tcctgctcct cggccacgaa gtgcacgcag
    10261 ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc
    10321 atggccggcc cggaggcgtc ccggaagttc gtggacacga cctccgacca ctcggcgtac
    10381 agctcgtcca ggccgcgcac ccacacccag gccagggtgt tgtccggcac cacctggtcc
    10441 tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc
    10501 acgaagtccc gggagaaccc gagccgg
//
