LOCUS       sequence-412356-        8386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent dopamine (DA) sensor 
            GRAB_rDA2h and a membrane-localized EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Zhuo Y, Luo B, Yi X, Dong H, Miao X, Wan J, Williams JT, Campbell 
            MG, Cai R, Qian T, Li F, Weber SJ, Wang L, Li B, Wei Y, Li G, Wang 
            H, Zheng Y, Zhao Y, Wolf ME, Zhu Y, Watabe-Uchida M, Li Y
  TITLE     Improved green and red GRAB sensors for monitoring dopaminergic 
            activity in vivo.
  JOURNAL   Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02100-w.
  PUBMED    38036855
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02100-w."
COMMENT     Sequence Label: pDisplay-GRAB-rDA2h-IRES-EGFP-CAAX
FEATURES             Location/Qualifiers
     source          1..8386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     540..560
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     634..653
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        634..652
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     733..792
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    796..820
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2277..2296
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2595..3146
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2739..2756)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2965..2984
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3158..3160,3161..3163,3164..3874)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3158..3160,3161..3163,3164..3874)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3203..3224)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3464..3483)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3811..3832
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3932..3961
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3965..4111
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4133..4150)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4139..4363
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4495..5083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4575..4594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5412..5459
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5484..5503
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5691..6485)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5691..6485)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5802..5821)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6412..6431
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6584..6603)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6682..6873
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6830..6850
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(6933..7724,7725..7793))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7556..7575
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7794..7898)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7925..8380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8012..8031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8222..8243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccctgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      421 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      481 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      541 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
      601 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
      661 agcttggtac cgagctcgga tccactagta acggccgcca gtgtgctgga attcggcttg
      721 gggatatcca ccatggagac agacacactc ctgctatggg tactgctgct ctgggttcca
      781 ggttccactg gtgacacaag tttgtacaaa aaagttggca ccaccggtat caaccgactg
      841 aactacagcg tcctcgtgaa cctgacggcc tactacgacg gcggcgcggg tctgaatctc
      901 agcagcgtca attgcacgtc gtcgatagtc gccgggggtg ccgttagacc gggcgagtgc
      961 gctggaaccg ccggcgtcga cgagaagtct aacgcgaaca gctggtgggc cctgatactt
     1021 gtgatcgtgc cgtgtttgac gctcttcggt aatgtgctcg tcatcctagc ggtggtgaaa
     1081 gaacgaacgc tgcagactgt cactaactac ttcatcgtca gtttggcggt cgccgatctt
     1141 ctcgtggcgg tgctcgttat gccgttcgcg gtctacgtgc tggtgaacgg atcctggagt
     1201 ttacccgggt ttgtttgtga tttttatatc gcaatggatg tgacttgcag caccagctct
     1261 atattcaatc tcgtggctat ttcaatagac agatacatag cggtgaccca gccgataaag
     1321 tacgcgaagc acaagaacaa tagaagagta tggttgacga tactgttggt ctgggcgata
     1381 tcggccgcga tcggcagccc gattgtccta ggcttgaata acactccgga ccggatcccg
     1441 gacgcatgtc tgttctacaa tacggatttc atcatctact cgagcctgtc cagcttctac
     1501 ataccctgca tcatcatggt attcctttac tacaatatat tcaaggctct acgaaacaga
     1561 gcgaaacttt ttcgtgctag ctgcgtgtgt tccgaggtta tgtaccccga ggacggcgcc
     1621 ctgaagagcg agatctggaa ggggctgagg ctgaaggacg gcggccacta cgccgccgag
     1681 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta catcgtcgac
     1741 atcaagttgg acatcgtgtc ccacaacgag gactacacca tcgtggaaca gtgcgaacgc
     1801 gccgaggccc gccactccac cggcggcatg gacgagctgt acaagggagg tacaggcggg
     1861 agtctggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     1921 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     1981 ggccgcccct acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg
     2041 cccttcactt gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacattaag
     2101 cacccagccg acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag
     2161 cgcgtgatga acttcgagga cggcggcatt attcacgtta accaggactc ctccctgcag
     2221 gacggctact tcatctacaa ggtgaagctg cgcggcacca acttcccccc cgacggcccc
     2281 gtaatgcaga agaagaccat gggctgggag ctgacggtcc tcgagcgcaa ggccagtaag
     2341 cccctggcga tcgtgttagg tgtctttcta atatgctggg tgcccttctt cacttgtaac
     2401 atcatggatg caatctgcac gaagctgacg aaggcttgtc agcctggcgt tacagccttc
     2461 atcgtcactt cctggttggg ctacatgaac agcttcgtaa atcccgtaat atacactgtg
     2521 ttcaaccccg aattccgtaa ggccttccgc aagctgatca gcatataagc tagcgcccct
     2581 ctccctcccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     2641 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     2701 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     2761 tctgttaaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     2821 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     2881 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     2941 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     3001 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt
     3061 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     3121 ttcctttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagct
     3181 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3241 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     3301 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     3361 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3421 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3481 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3541 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3601 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     3661 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     3721 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     3781 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3841 cgggatcact ctcggcatgg acgagctgta caaggatggt aaaaagaaga aaaagaagtc
     3901 aaagacaaag tgtgtaatta tgtaagtcga cgaacaaaaa ctcatctcag aagaggatct
     3961 gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg ccacactcct tgccctttaa
     4021 ggtggtggtg atctcagcca tcctggccct ggtggtgctc accatcatct cccttatcat
     4081 cctcatcatg ctttggcaga agaagccacg ttaggcggcc gctcgagatc agcctcgact
     4141 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4201 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4261 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4321 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4381 accagtggcg gtaatacggt tatccacaga atcgggggat aacgcaggaa agaacatgtg
     4441 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4501 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4561 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4621 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4681 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4741 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4801 tcttgagtcc aacccggtaa gacgcgactt atcgccactg gcagcagcca ctggtaacag
     4861 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4921 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4981 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5221 ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc
     5281 gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
     5341 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
     5401 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt
     5461 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg
     5521 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     5581 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     5641 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
     5701 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     5761 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     5821 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     5881 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     5941 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     6001 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     6061 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     6121 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     6181 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     6241 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     6301 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     6361 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     6421 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     6481 atcatgcgaa acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc
     6541 aaaaaagcct cctcactact tctggaatag ctcagaggcc gggcctcggc ctctgcataa
     6601 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     6661 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     6721 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     6781 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     6841 cacacattcc acagctggtt ctttccgcct caggactctt cctttttcaa taaatcaatc
     6901 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6961 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7021 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7081 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7141 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7201 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7261 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7321 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7381 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7441 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7501 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7561 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7621 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7681 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7741 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7801 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7861 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7921 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7981 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8041 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8101 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8161 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8221 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8281 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8341 tttaacgcga attttaacaa aatattaacg cttacaattt acgcgc
//
