LOCUS       sequence-412359-        8602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent dopamine (DA) sensor 
            GRAB_rDA3h and a membrane-localized EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Zhuo Y, Luo B, Yi X, Dong H, Miao X, Wan J, Williams JT, Campbell 
            MG, Cai R, Qian T, Li F, Weber SJ, Wang L, Li B, Wei Y, Li G, Wang 
            H, Zheng Y, Zhao Y, Wolf ME, Zhu Y, Watabe-Uchida M, Li Y
  TITLE     Improved green and red GRAB sensors for monitoring dopaminergic 
            activity in vivo.
  JOURNAL   Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02100-w.
  PUBMED    38036855
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02100-w."
COMMENT     Sequence Label: pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX
FEATURES             Location/Qualifiers
     source          1..8602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        18..397
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        398..601
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     552..572
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     646..665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        646..664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2823..3374
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2967..2984)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3193..3212
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3386..3388,3389..3391,3392..4102)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3386..3388,3389..3391,3392..4102)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3431..3452)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3692..3711)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4039..4060
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4160..4189
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4193..4339
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4361..4378)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4367..4591
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4723..5311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4803..4822)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5640..5687
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5712..5731
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5919..6713)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5919..6713)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6030..6049)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6640..6659
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6812..6831)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6910..7101
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7058..7078
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7161..7952,7953..8021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7784..7803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8022..8126)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8153..8581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8240..8259)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8450..8471
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caatttacgc gcgcgttgac attgattatt gactagttat taatagtaat caattacggg
       61 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      121 gcctggctga ccgcccaacg acccctgccc attgacgtca ataatgacgt atgttcccat
      181 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      241 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      301 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      361 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      421 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      481 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      541 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      601 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
      661 tagggagacc caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg
      721 gaattcggct tggggatatc caccatgaag acgatcatcg ccctgagcta catcttctgc
      781 ttggtgttcg ccgactacaa ggacgatgat gacgccatga ggactctgaa cacctctgcc
      841 atggacggga ctgggctggt ggtggagagg gacttctctg ttcgtatcct cactgcctgt
      901 ttcctgtcgc tgctcatcct gtccacgctc ctggggaaca cgctggtctg tgctgccgtt
      961 atcaggttcc gacacctgcg gtccaaggtg accaacttct ttgtcatctc cttggctgtg
     1021 tcagatctct tgatcgccgt cctggtcatg ccctggaagg cagtggctga gattgctggc
     1081 ttctggccct ttgggtcctt ctgtaacatc tgggtggcct ttgacatcat gtgctccact
     1141 gcatccatcc tcaacctctg tgtgatcagc gtggacaggt attgggctat ctccagccct
     1201 gagcggtatg agagaaagat gacccccaag gcagccttca tcctgatcag tgtggcatgg
     1261 accttgtctg tactcatctc cttcatccca gtgcagctca gctggcacaa ggcaaaaccc
     1321 acaagcccct ctgatggaaa tgccacttcc ctggctgaga ccatagacaa ctgtgactcc
     1381 agcctcagca ggacatatgc catctcatcc tctgtaataa gcttttacat ccctgtggcc
     1441 atcatgattg tctgctacac caggatctac aggattgctc agaaaaagct ctcctccttc
     1501 atggtttccg agcggatgta ccccgaggac ggcgccctga agagcgagat caagaagggg
     1561 ctgaggctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
     1621 aagcccgtgc agctgcccgg cgcctacatc gtggacatca agttggacat cgtgtcccac
     1681 aacgaggact acaccatcgt ggaacagtgc gaacgcgccg agggccgcca ctccaccggc
     1741 ggcatggacg agctgtacaa gggaggtaca ggcgggagtc tggtgagcaa gggcgaggag
     1801 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     1861 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga ggcctttcag
     1921 accgctaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     1981 cctcagttca tgtacggctc caaggcctac attaagcacc cagccgacat ccccgactac
     2041 ttcaagtcgt ccttccccga gggcttcagg tgggagcgcg tgatgaactt cgaggacggc
     2101 ggcattattc acgttaacca ggactcctcc ctgcaggacg gcgtattcat ctacaaggtg
     2161 aagctgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa aactatgggg
     2221 tgggaacatc ggaaggacga ccttaaaaga gaaactaaag tcctgaagac tctgtcggtg
     2281 atcatgggtg tgtttgtgtg ctgttggcta cctttcttca tcttgaactg cattttgccc
     2341 ttctgtgggt ctggggagac gcagcccttc tgcattgatt ccaacacctt tgacgtgttt
     2401 gtgtggtttg ggtgggctaa ttcatccttg aaccccatca tttatgcctt taatgctgat
     2461 tttcggaagg cattttcaac cctcttagga tgctacagac tttgccctgc gacgaataat
     2521 gccatagaga cggtgagtat caataacaat ggggccgcga tgttttccag ccatcatgag
     2581 ccacgaggct ccatctccaa ggagtgcaat ctggtttacc tgatcccaca tgctgtgggc
     2641 tcctctgagg acctgaaaaa ggaggaggca gctggcatcg ccagaccctt ggagaagctg
     2701 tccccagccc tatcggtcat attggactat gacactgacg tctctctgga gaagatccaa
     2761 cccatcacac aaaacggtca gcacccaacc tgataagcta gcgcccctct ccctcccccc
     2821 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     2881 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     2941 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttaaatgt
     3001 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     3061 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa gccacgtgta
     3121 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     3181 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     3241 gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag
     3301 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     3361 acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt
     3421 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3481 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3541 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     3601 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3661 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3721 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3781 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3841 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3901 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3961 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     4021 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4081 cggcatggac gagctgtaca aggatggtaa aaagaagaaa aagaagtcaa agacaaagtg
     4141 tgtaattatg taagtcgacg aacaaaaact catctcagaa gaggatctga atgctgtggg
     4201 ccaggacacg caggaggtca tcgtggtgcc acactccttg ccctttaagg tggtggtgat
     4261 ctcagccatc ctggccctgg tggtgctcac catcatctcc cttatcatcc tcatcatgct
     4321 ttggcagaag aagccacgtt aggcggccgc tcgagatcag cctcgactgt gccttctagt
     4381 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4441 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4501 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4561 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagtggcggt
     4621 aatacggtta tccacagaat cgggggataa cgcaggaaag aacatgtgag caaaaggcca
     4681 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4741 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4801 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4861 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4921 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4981 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5041 cccggtaaga cgcgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5101 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5161 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5221 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5281 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5341 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5401 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5461 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     5521 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     5581 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     5641 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     5701 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     5761 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     5821 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     5881 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     5941 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     6001 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     6061 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     6121 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     6181 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     6241 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     6301 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     6361 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     6421 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     6481 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     6541 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     6601 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     6661 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     6721 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
     6781 tcactacttc tggaatagct cagaggccgg gcctcggcct ctgcataaat aaaaaaaatt
     6841 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     6901 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     6961 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     7021 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     7081 agctggttct ttccgcctca ggactcttcc tttttcaata aatcaatcta aagtatatat
     7141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7261 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7681 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7981 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     8161 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8221 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8281 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8341 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8401 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8461 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8521 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8581 tttaacaaaa tattaacgct ta
//
