LOCUS       sequence-412366-        6986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LRP1 protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6986)
  TITLE     TREAT-AD plasmids Jun 2023
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LRP1A-c004
FEATURES             Location/Qualifiers
     source          1..6986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     33..92
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..122
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             147..167
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             648..692
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             801..821
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             822..851
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             852..875
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1033..1167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1070..1089)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1124..1143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1196..1361
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1545..1999
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1632..1651)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1841..1862
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2025..2129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2130..2198,2199..2990)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2348..2367)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3161..3749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3650..3669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3903..3920
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4054..4278
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4345..4878)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5048..5053,5054..5070,5071..5076))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    5585..5633
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5658..5677
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5708..5731)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(5741..6703)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNHVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     promoter        complement(6738..6847)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        6871..6962
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     6887..6904
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 accatcgggc gcgcttaaga aggagatata ctatggtaag cgctattgtt ttatatgtgc
       61 ttttggcggc ggcggcgcat tctgcctttg cggccgccat gggccaccat catcatcatc
      121 attcttctgg tgtagatctg ggtaccgaga acctgtactt ccaatccatg gctatcgacg
      181 cccctaagac ttgcagcccc aagcagtttg cctgcagaga tcaaataacc tgtatctcaa
      241 agggctggcg gtgcgacggt gagagggact gcccagacgg atctgacgag gcccctgaga
      301 tttgtccaca gagtaaggcc cagcgatgcc agccaaacga gcataactgc ctgggtactg
      361 agctgtgtgt tcccatgtcc cgcctctgca atggggtcca ggactgcatg gacggctcag
      421 atgaggggcc ccactgccga gagctccaag gcaactgctc tcgcctgggc tgccagcacc
      481 attgtgtccc cacactcgat gggcccacct gctactgcaa cagcagcttt cagcttcagg
      541 cagatggcaa gacctgcaaa gattttgatg agtgctcagt gtacggcacc tgcagccagc
      601 tatgcaccaa cacagacggc tccttcataa gcagtaaagg tggatacggc ctgaatgata
      661 tctttgaagc gcagaagatt gaatggcatg aatgataagc agagacctct acttccaatc
      721 gggtctgaac gacatcttcg aagctcagaa aattgaatgg cactgaggat ccgaattcga
      781 gctccgtcga caattgggca gagaacctct acttccaatc gcaccatcat caccatcacc
      841 atcaccacca tgattacaag gatgacgacg ataagtgagg atccgaattc gagctccgtc
      901 gacaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt
      961 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     1021 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1081 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1141 atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctaggag atccgaacca
     1201 gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga
     1261 cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca
     1321 tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat ttttcttcct
     1381 gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat cttcggctac
     1441 tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa tgtttgtaat
     1501 tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     1561 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     1621 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     1681 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     1741 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     1801 ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt gttccaaact
     1861 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     1921 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     1981 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2041 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2101 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2161 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2221 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2281 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2341 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2401 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2461 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2521 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2581 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2641 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2701 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2761 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2821 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2881 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2941 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3001 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3061 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3121 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3181 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3241 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3301 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3361 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3421 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3481 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3541 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3601 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3661 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3721 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3781 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3841 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3901 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3961 atctgtgcgg tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg
     4021 ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact
     4081 gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac
     4141 tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa
     4201 actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc
     4261 atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
     4321 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
     4381 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
     4441 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga gcagattgat
     4501 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     4561 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     4621 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     4681 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     4741 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     4801 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     4861 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     4921 ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     4981 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     5041 gcattacagt ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt
     5101 ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg
     5161 gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt caggcattgg cggccttgct
     5221 gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga tcggtagacc
     5281 tcggccgtcg cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg
     5341 ttttctggaa ggcgagcatc gtttgttcgc ccaggactct agctatagtt ctagtggttg
     5401 gcctacgtac ccgtagtggc tatggcaggg cttgccgccc cgacgttggc tgcgagccct
     5461 gggccttcac ccgaacttgg gggttggggt ggggaaaagg aagaaacgcg ggcgtattgg
     5521 tcccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt
     5581 ttatgaacaa acgacccaac acccgtgcgt tttattctgt ctttttattg ccgtcatagc
     5641 gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc atctcccgtc
     5701 tagatcactt atcgtcatcg tctttgtaat ccgaattccc tttttctgca ctacgcaggg
     5761 atatttcacc gcccatccag ggttttatta ttccatcctg ctcaagtaat aaagccccct
     5821 gtttgtctat tccgcgtgaa atgccaaata tttctttatc accaatgata agtttcactg
     5881 ggcgattaat aaaattatcc agcttttccc agcgcgacag ataaggtgcc aatccttctt
     5941 gttcgaagag ttccaacgca gcacgtaatt cacgtattag catggccgcc aacgtattac
     6001 gatcgagatt gatccccgct tcctgcagcg tgatccaccc ctgattaacg acactctctt
     6061 caacacggcg cattgccatg ttgatcccgg ctccaatgac tatttgcgcc gcatcgccag
     6121 ttttgccagt cagctccacc agaatgcctg ccagcttgcg atcctgcaga tagaggtcat
     6181 taggccattt aacacgaact ttatctgcac ccagcttgcg taatacttcc gccatcacga
     6241 taccgataac cagacttaaa ccaatcgccg ccgccgggcc ttgttccaga cgccagaaca
     6301 tcgacaaata taagtttgcg ccaaaaggcg aaaaccattt ccgaccccgg cgaccacggc
     6361 cagcctgctg gtattctgca atgcaagcat cgcccgattt aagctctccg atacgatcaa
     6421 gaaggtactg attcgtggag tcaatcactg gcagcacggc tacactaccg ccatccagct
     6481 gacccaatat ctgtttagca ttaagtaact ggataggctc aggcaggctg tatcctttac
     6541 ccggaacggt aaagacatca acgccccagt cacgcagtgt ctgaatgtgt ttattaatag
     6601 ccgcccggct cattcccagc gtttcaccca actgctcgcc agagtgaaat tcaccgttcg
     6661 ctaacagggc aatcaatttc agtggcacgt ggttatcctt catctcgaga tcccgggtga
     6721 tcaagtcttc gtcgagtgat tgtaaataaa atgtaattta cagtatagta ttttaattaa
     6781 tatacaaatg atttgataat aattcttatt taactataat atattgtgtt gggttgaatt
     6841 aaaggtccgt atactccgga atattaatag atcatggaga taattaaaat gataaccatc
     6901 tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
     6961 atattccgga ttattcatac cgtccc
//
