LOCUS       sequence-412367-       10527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting 
            murine ZBP1#2 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, 
            Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, 
            Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis 
            JE, Gupta GP
  TITLE     MRE11 liberates cGAS from nucleosome sequestration during 
            tumorigenesis.
  JOURNAL   Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6.
  PUBMED    38200309
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Jan 10. doi: 10.1038/s41586-023-06889-6."
COMMENT     Sequence Label: Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#2
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    145..266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(182..201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     236..255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(315..331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(315..331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(328..350)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    339..355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(363..369,370..387,388..393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    408..429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(546..563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(717..1305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(797..816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1476..2267,2268..2336))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2099..2118
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2337..2441)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2516..2535)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2707..3086
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3088..3286
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3241..3261
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3304..3484
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3531..3656
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4149..4382
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4567..4611
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4909..5026
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5077..5325
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5077..5097
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5248..5267
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5347..5422
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        5484..5695
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5721..5741
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5742..6767
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLQ
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             6768..6824
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6825..6827,6828..6830,6831..7541)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6825..6827,6828..6830,6831..7541)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7131..7150)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7478..7499
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7557..8072
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    8088..8676
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8141..8161)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8559..8570)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8748..8981
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9007..9024)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9013..9237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9283..9711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9370..9389)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9580..9601
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9720..9740)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9725..10054
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9905..10040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9967..9986
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10102..10149
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(10168..10527,1..15)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
       61 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
      121 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
      181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      241 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
      301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      361 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      781 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      841 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      901 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      961 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1021 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1081 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1141 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1201 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1261 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1321 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1381 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1441 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1501 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1561 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1621 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1681 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1741 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1801 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1861 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1921 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1981 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2041 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2101 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2161 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2221 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2281 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2461 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     2521 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     2581 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     2641 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     2701 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     2761 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2821 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2881 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2941 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3001 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3061 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     3121 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3181 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     3241 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
     3301 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     3361 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     3421 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     3481 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     3541 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     3601 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     3661 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     3721 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     3781 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     3841 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     3901 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     3961 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     4021 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     4081 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     4141 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     4201 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     4261 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     4321 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     4381 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     4441 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     4501 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     4561 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     4621 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     4681 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     4741 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     4801 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     4861 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     4921 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     4981 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     5041 ggacagcaga gatccagttt ggttaattaa ggtaccgagg gcctatttcc catgattcct
     5101 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     5161 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     5221 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     5281 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgacct cttccttcac
     5341 ctcgcggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     5401 aaaagtggca ccgagtcggt gcttttttga attcgctagc taggtcttga aaggagtggg
     5461 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     5521 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     5581 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     5641 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggaccg
     5701 gttctagagg atctaccatg cccaagaaga agaggaaggt gtccaattta ctgaccgtac
     5761 accaaaattt gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aagaacctga
     5821 tggacatgtt cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttcagtccg
     5881 tttgccggtc gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac
     5941 ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg cggtctggca gtaaaaacta
     6001 tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa
     6061 gtgacagcaa tgctgtttca ctggttatgc ggcggatccg aaaagaaaac gttgatgccg
     6121 gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga tttcgaccag gttcgttcac
     6181 tcatggaaaa tagcgatcgc tgccaggata tacgtaatct ggcatttctg gggattgctt
     6241 ataacaccct gttacgtata gccgaaattg ccaggatcag ggttaaagat atctcacgta
     6301 ctgacggtgg gagaatgtta atccatattg gcagaacgaa aacgctggtt agcaccgcag
     6361 gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt cgagcgatgg atttccgtat
     6421 ctggtgtagc tgatgatccg aataactacc tgttttgccg ggtcagaaaa aatggtgttg
     6481 ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa
     6541 ctcatcgatt gatttacggc gctaaggatg actctggtca gagatacctg gcctggtctg
     6601 gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg cgctggagtt tcaataccgg
     6661 agatcatgca agctggtggc tggaccaatg taaatattgt catgaactat atccgtaacc
     6721 tggatagtga aacaggggca atggtgcgcc tgctggaaga tggcgatgca acaaacttct
     6781 ctctgctgaa acaagccgga gatgtcgaag agaatcctgg accgatggtg agcaagggcg
     6841 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     6901 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     6961 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     7021 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     7081 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     7141 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     7201 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     7261 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     7321 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     7381 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     7441 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     7501 ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggatatctcc ggaggtatgg
     7561 tcttcacact cgaagatttc gttggggact ggcgacagac agccggctac aacctggacc
     7621 aagtccttga acagggaggt gtgtccagtt tgtttcagaa tctcggggtg tccgtaactc
     7681 cgatccaaag gattgtcctg agcggtgaaa atgggctgaa gatcgacatc catgtcatca
     7741 tcccgtatga aggtctgagc ggcgaccaaa tgggccagat cgaaaaaatt tttaaggtgg
     7801 tgtaccctgt ggatgatcat cactttaagg tgatcctgca ctatggcaca ctggtaatcg
     7861 acggggttac gccgaacatg atcgactatt tcggacggcc gtatgaaggc atcgccgtgt
     7921 tcgacggcaa aaagatcact gtaacaggga ccctgtggaa cggcaacaaa attatcgacg
     7981 agcgcctgat caaccccgac ggctccctgc tgttccgagt aaccatcaac ggagtgaccg
     8041 gctggcggct gtgcgaacgc attctggcgt aaacgcgtta agtcgacaat caacctctgg
     8101 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     8161 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     8221 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     8281 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8341 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8401 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8461 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     8521 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8581 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     8641 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     8701 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8761 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     8821 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8881 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8941 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     9001 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     9061 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     9121 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     9181 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     9241 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     9301 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9361 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9421 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9481 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     9541 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9601 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9661 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     9721 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9781 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     9841 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     9901 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9961 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
    10021 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
    10081 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
    10141 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
    10201 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
    10261 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
    10321 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
    10381 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
    10441 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
    10501 gccggcaact gcgtgcactt cgtggcc
//
