LOCUS       sequence-412374-       11021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To generate stable cell line expressing human cGAS(wt) using 
            PiggyBac transposon system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, 
            Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, 
            Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis 
            JE, Gupta GP
  TITLE     MRE11 liberates cGAS from nucleosome sequestration during 
            tumorigenesis.
  JOURNAL   Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6.
  PUBMED    38200309
REFERENCE   2  (bases 1 to 11021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Jan 10. doi: 10.1038/s41586-023-06889-6."
COMMENT     Sequence Label: PB-TA-ERP2-hcGAS(wt)
FEATURES             Location/Qualifiers
     source          1..11021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    103..124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(139..144,145..162,163..169)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    177..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     182..204
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     201..217
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     201..217
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        238..256
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   405..439
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    813..1385
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     CDS             complement(1471..2070)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1554..1574)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2051..2070
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    join(2071..2079,2080..2082,2083..2657)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2244..2263)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2473..2490
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(2684..2692,2693..2728,2729..2731,2732..2767,
                     2768..2770,2771..2806,2807..2812,2813..3430))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(3447..4752)
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    3479..4410
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     enhancer        4867..5246
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5671..5690
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5689..5744
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5790..5809
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     protein_bind    5924..5948
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7521..7545)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7624..7648)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7670..7688
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7707..7725
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7744..7762
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7781..7799
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7818..7836
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7855..7873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7892..7910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7929..7947
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     repeat_region   8319..8381
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8447..8466)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8448..8466)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8473..8490)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8473..8489)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8482..8504)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8631..9086
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8718..8737)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8928..8949
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9112..9216
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9217..9285,9286..10077)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9435..9454)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10248..10836
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10737..10756
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10990..11007
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
       61 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      121 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      181 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat
      241 taaccctcac taaagggaac aaaagctggt acctcgcgcg acttggtttg ccattcttta
      301 gcgcgcgtcg cgtcacacag cttggccaca atgtggtttt tgtcaaacga agattctatg
      361 acgtgtttaa agtttaggtc gagtaaagcg caaatctttt ttaaccctag aaagatagtc
      421 tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc tctttctaaa tagcgcgaat
      481 ccgtcgctgt gcatttagga catctcagtc gccgcttgga gctcccgtga ggcgtgcttg
      541 tcaatgcggt aagtgtcact gattttgaac tataacgacc gcgtgagtca aaatgacgca
      601 tgattatctt ttacgtgact tttaagattt aactcatacg ataattatat tgttatttca
      661 tgttctactt acgtgataac ttattatata tatattttct tgttatagat atcgtgacta
      721 atatataata aaatgggtag ttctttagac gatgagcata tcctctctgc tcttctgcaa
      781 agcgatgacg agcttgttgg ctagcgtttg gcagctttct atgcaaccca aggactcagt
      841 ttttggcctg ttttagtgac aggcaatcag caacatgctg catttctctc cagtgttgta
      901 atcaaagcaa ccctcccata gctttaaatg atattccttc cccttccaat tatgtggggg
      961 gaaaacaacc ctattctcca cccagaagtg ttaactcaag aattacattt tcaagaagtt
     1021 tccagattcg taaaaccaga attagatgtc tttcacctaa atgtctcggt gttgaccaaa
     1081 ggaacacaca ggtttctcat ttaacttttt taatgggtct caaaattctg tgacaaattt
     1141 ttggtcaagt tgtttccatt aaaaagtact gattttaaaa actaataact taaaactgcc
     1201 acacgcaaaa aagaaaacca aagtggtcca caaaacattc tcctttcctt ctgaaggttt
     1261 tacgatgcat tgttatcatt aaccagtctt ttactactaa acttaaatgg ccaattgaaa
     1321 caaacagttc tgagaccgtt cttccaccac tgattaagag tggggtggca ggtattaggg
     1381 ataatcctag cgaattcgct tggtcggtca tttcgatgca tggggtcgtg cgctcctttc
     1441 ggtcgggcgc tgcgggtcgt ggggcgggcg tcaggcaccg ggcttgcggg tcatgcacca
     1501 ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta
     1561 gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc
     1621 cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga
     1681 gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc
     1741 ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat
     1801 ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc
     1861 gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc
     1921 ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg
     1981 gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc
     2041 gaggcgcacc gtgggcttgt actcggtcat ggttgtggcc atattatcat cgtgtttttc
     2101 aaaggaaaac cacgtccccg tggttcgggg ggcctagacg tttttttaac ctcgactaaa
     2161 cacatgtaaa gcatgtgcac cgaggcccca gatcagatcc catacaatgg ggtaccttct
     2221 gggcatcctt cagccccttg ttgaatacgc ttgaggagag ccatttgact ctttccacaa
     2281 ctatccaact cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta tcttatacac
     2341 gtggcttttg gccgcagagg cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg
     2401 tcgctacaga cgttgtttgt cttcaagaag cttccagagg aactgcttcc ttcacgacat
     2461 tcaacagacc ttgcattcct ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga
     2521 agacagggcc aggtttccgg gccctcacat tgccaaaaga cggcaatatg gtggaaaata
     2581 acatatagac aaacgcacac cggccttatt ccaagcggct tcggccagta acgttagggg
     2641 ggggggaggg agaggggggg ggggcggaat gatccttact tagttacccg gggagcatgt
     2701 caaggtcaaa atcgtcaaga gcgtcagcag gcagcatatc aaggtcaaag tcgtcaaggg
     2761 catcggctgg gagcatgtct aagtcaaaat cgtcaagggc gtcggccggc ccgccgcttt
     2821 cgcactttag ctgtttctcc aggccacata tgattagttc caggccgaaa aggaaggcag
     2881 gttcggctcc ctgccggtcg aacagctcaa ttgcttgtct cagaagtggg ggcatagaat
     2941 cggtggtagg tgtctctctt tcctcttttg ctacttgatg ctcctgttcc tccaatacgc
     3001 agcccagtgt aaagtggccc acggcggaca gagcgtacag tgcgttctcc agggagaagc
     3061 cttgctgaca caggaacgcg agctgatttt ccagggtttc gtactgtttc tctgttgggc
     3121 gggtgccgag atgcacttta gccccgtcgc gatgtgagag gagagcacag cggaatgact
     3181 tggcgttgtt ccgcagaaag tcttgccatg actcgccttc cagggggcag aagtgggtat
     3241 gatgcctgtc cagcatctcg attggcaggg catcgagcag ggcccgcttg ttcttcacgt
     3301 gccagtacag ggtaggctgc tcaactccca gcttttgagc gagtttcctt gtcgtcaggc
     3361 cttcgatacc gactccattg agtaattcca gagcgccgtt tatgactttg ctcttgtcca
     3421 gtctagacat ggtgaattac tccggattgc tttgaattag cggtggcttt cacaacacct
     3481 ggaaacgggt ttagaaaata cctttgaacc gctaagaggc ttgagaatta gcctcgctcc
     3541 aaactcaaag gggcaaattc caagaatgac ttccagcgcc aggctggcct gactagtctc
     3601 cacccaccaa atgtgaacaa actccaacgc cattagatcc cctccccccg ccgcgacgag
     3661 cggtgctcaa gagaagcccg aggtgactat gcggccgata gaccacgggg tcacaggaag
     3721 cagcggccgg cgaggggcaa ggccctcttc ctttgtgtgg gtgactcacc cgcccgctcc
     3781 accgggctgc cgcgtcctcc attttgagct ccttgcaaca gggccccggg agcggccatc
     3841 tttccgcgca agcaactggt gccgggcggg acggcctcac ccttgttggg aggcaaggga
     3901 acgcggcacc gccaggccag accgtgccca gtgctagggc cacatggccc aggtaggcta
     3961 aactcacgcc cgattgcttc ggaaagccac aggcctcgcc ccatccaggt ggcgtcggac
     4021 atgtgctccg aaggcgggcg ggccccagtc gcccctcctg cccttccatt cctatccaac
     4081 gtaagcttca tgggctcctt aagagtgagc cccacaccct ccctttgtta gcccatgctg
     4141 ctggaaggca atcgagggca gcaaggacaa aaaacgcata gtgcggccgg agtggcacgc
     4201 ggcggcggac accacgctag gcctcaagcc ggacacgagg cgaggctacg gggttgccgc
     4261 taggcctcgc actctcaccc ccgcaccgcc cgccactcga agtgggaatg ctcacagcta
     4321 aaccctgcgg acgacactcg catatcagcc cggcccgctc agagcagggc tccagctccg
     4381 gggcccgcgg aagccacacc cgccactcac ctgcgttctg acggcaaacc cgttgcgaaa
     4441 aagaacgtcc aaggcgacta ccgcacttat ataccgttct cccccaccct cgggaagagg
     4501 gcggagccag cacacgacac cactttccca gtttgccccg cgccagctcc ctggcccccg
     4561 ctcagcgcgc ccctcccccc accgccgggg actgtgagct tgtgtgctct gatctacccg
     4621 cgctcagccc tgggcgcatg ctcctcgcgc tgtcgtgggg cggctacagg gatttgggtc
     4681 tgtgagaatt tagggacggt ccctgggctc tccccttgcg aaggcgatcc ctccttttgt
     4741 atgaattact ctcggctcca ctagtcaaaa caaactccca ttgacgtcaa tggggtggag
     4801 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     4861 catcatcatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     4921 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     4981 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     5041 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     5101 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     5161 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     5221 ttaataacta gtcaataatc aatgtcgagg ccgccaccgc tagtgcggcc gctgcagaag
     5281 cttccatggt taacgaattc atcgattcga atgtacagat ctggatccta ggcttaagta
     5341 atcgaattcc tgcaggtcga gggatctcca taagagaaga gggacagcta tgactgggag
     5401 tagtcaggag aggaggaaaa atctggctag taaaacatgt aaggaaaatt ttagggatgt
     5461 taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc taacctcaag tcaaggcttt
     5521 tctatggaat aaggaatgga cagcaggggg ctgtttcata tactgatgac ctctttatag
     5581 ccaacctttg ttcatggcag ccagcatatg ggcatatgtt gccaaactct aaaccaaata
     5641 ctcattctga tgttttaaat gatttgccct cccatatgtc cttccgagtg agagacacaa
     5701 aaaattccaa cacactattg caatgaaaat aaatttcctt tattagccag aagtcagatg
     5761 ctcaaggggc ttcatgatgt ccccataatt tttggcagag ggaaaaagat ctcagtggta
     5821 tttgtgagcc agggcattgg ccacaccagc caccaccttc tgataggcag cctgcacctg
     5881 aggagtgaat tcgatggggg atcccttcgc tagagcggcc atcaccactt tgtacaagaa
     5941 agctgggttt caaaattcat caaaaactgg aaactcattg tttctttcat attcaatttg
     6001 ctttgtcaga aattctttac ttcttttgtc aattaagttg ctagagaata gattgaattc
     6061 aggaataaaa taattctcaa gtttttctgt cctgaggcac tgaagaaagt atgtcacgca
     6121 gttatcaaag cagaggccca ggtctttgcg gtcccactga ctgtcttgag ggttctgggt
     6181 acatacgtga aagaaggcag ttttcacatg ataagaagag aatttatcca gatgtttttt
     6241 gtctttaaac ctttctttca gctgttctaa aaggtatttc attagtttta aacaatcttt
     6301 cctgcaacat ttctcttctt tgttttcaca gcacgtttta gattttccat gattgttcaa
     6361 aatttccttt tcgatgtgag agaaggatag ccgccatgtt tcttcttgga aaccatttcc
     6421 ttcctttgca tgcttgggta caaggtaaaa tggctttagt cgtagttgct tcctaacttt
     6481 tgctgaaagc cagttttgaa tgcgcaggcc ttcttgggtg ctagcaggcc agctactttt
     6541 tgattccaaa gccagggtta tatccacaga tattttttca ctaataagaa gtgttacagc
     6601 agggctccct cctcttttcc tcttcatgat gacatctgta tctttaatgt cgttaatttc
     6661 ttccttaatg attttcctaa actttgacag catcttagaa gctgataata tttcaccttc
     6721 taaaaactga ctcagatgat tttctttcgg atttctttta aatttcacaa agtaatatgc
     6781 acgagtgttg gaatattctt ctagttgaat tctggggact tccagtttaa acatgacatc
     6841 aaattcatta ggtgcagaaa tcttcacgtg ctcatagtag ctcccggtgt tcagcagccc
     6901 gacgcctctg aacgcggagt cgcacttcag tctgagcagc aggtggtcca caaccccttt
     6961 caccatcccc gccgccgtgg agatatcatc gcggctgagc ttcaacttct ccaaaaccgc
     7021 ccggagcttc gaggccccag gcgccgcatc cctccgtacg agaatggggg ccgagaccgg
     7081 caggccgggg ctgggcacgt cccagggccc gggcgggggt cttggcttcg tggagcagcg
     7141 cgcgcccctc tggcggcaag aaccagccct ggaaagagcc ggctcccgag ccgcaggagg
     7201 ctccagcccc tctgccccag gggcgctggt ggcgtcagac ggctgcgtgt cctgggcgcg
     7261 ctgaggggcc tttttggcgc gggccccagt tgcgcggacg ggcggcctct cctgggtgtc
     7321 cggggcgctc tttttctgcc gggatcccga cttcctggcg gggccgaact ttcccgcctt
     7381 aggcagggcg gcctcggggg cagccggaga ctcgttggga tccatcgggg cgcccctggc
     7441 attccgtgcg gaagccttgg gggcagtggc tccggcctcg gaagctctct gcatggcctt
     7501 tccgtgccaa ggctgcatga agcctgcttt tttgtacaaa cttgtgatgc cgccaccgcg
     7561 gaggctggat cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca
     7621 ggcgatctga cggttcacta aacgagctct gcttatatag ggggtacctt ctctatcact
     7681 gatagggagc tcgagacgtg tcgacttctc tatcactgat agggagacgt tctagacgta
     7741 ccttctctat cactgatagg gagctcgaga cgtgtcgact tctctatcac tgatagggag
     7801 acgttctaga cgtaccttct ctatcactga tagggagctc gagacgtgtc gacttctcta
     7861 tcactgatag ggagacgttc tagacgtacc ttctctatca ctgataggga gctcgagacg
     7921 tgtcgacttc tctatcactg atagggagac gtgcggccag cttggatccc tcgagttaat
     7981 taacgagagc ataatattga tatgtgccaa agttgtttct gactgactaa taagtataat
     8041 ttgtttctat tatgtatagg ttaagctaat tacttatttt ataatacaac atgactgttt
     8101 ttaaagtaca aaataagttt atttttgtaa aagagagaat gtttaaaagt tttgttactt
     8161 tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg
     8221 tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa
     8281 attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacgcatga
     8341 ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaaataat agtttctaat
     8401 ttttttatta ttcagcctgc tgtcgtgaat accgagctcc aattcgccct atagtgagtc
     8461 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8521 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8581 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     8641 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8701 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8761 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8821 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8881 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8941 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9001 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     9061 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     9121 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9181 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9241 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9301 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9361 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9421 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9481 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9541 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9601 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9661 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9721 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9781 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9841 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9901 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9961 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10021 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10081 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10141 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10201 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10261 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10321 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10381 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10441 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10501 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10561 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10621 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10681 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10741 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10801 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10861 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10921 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10981 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc g
//
